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The map label for this gene is entE [H]

Identifier: 163844213

GI number: 163844213

Start: 12084

End: 13703

Strand: Reverse

Name: entE [H]

Synonym: BSUIS_B0017

Alternate gene names: 163844213

Gene position: 13703-12084 (Counterclockwise)

Preceding gene: 163844214

Following gene: 163844212

Centisome position: 0.98

GC content: 60.86

Gene sequence:

>1620_bases
ATGACCATCGAATTTACCCATTGGCCACCGGCACGCGAACGCCTCTATCGCGAAAAGGGATACTGGATCGACAAACCCCT
CACCCGCGCACTGGAAGAACAGGCCGCCATGCGGCCCGATGCGCCCGCTATCCTCTGCGGCGAGCGGCGTTTCACCTATG
CCGAACTCGACCGTCTGTCTTCCAACCTGGCCTCCCGGCTTGCGGCAGCCGGCATAGGCAAAGGCGACACGGCACTGGTG
CAACTGCCCAATATCGCCGAATTCTACATCGTGTTCTTCGCACTCATGAAAGCGGGCATCGCGCCCGTCAATGCGCTTTT
CAGCCATCGCAAGCTGGAGCTTGGCGCCTATGCCAGCCAGATCGAACCACGGCTGCTGATCGGCTCACGCCAGCATGAAC
TCTTCATGGATGACGCCTTTGCCCGCGACCTTGGCAAAAATCTTTCCGCACCGCTGCTAACACTTTTTGCAGGCGAGGCC
GACCCTGCGTCGAGCCTCGACCACTGGATCGCCACGCCTGCGGACAAAGCCGTTCCCTTCTCGCCAACCGGCGCTGGCGA
AGTCGCCTTTTTCCAGCTTTCCGGCGGCAGCACCGGCACGCCGAAACTCATCCCGCGCACGCATAATGATTATGATTACA
GCGTGCGCGCCAGCGCGGAAATCTGCGCGCTGACACCGCAGACCCGTTTTCTCTGCGCCCTGCCCACCGCGCATAATTAT
CCAATGAGTTCGCCCGGCGCGCTCGGCGTTTTCCATGCGGGCGGCTGTGTCGTCATGGCCTCCAACCCGGAACCGCTCAA
CTGCTTCTCCATCATCGAACGTCATGGCGTAAACATGGTGGCACTGGTGCCTCCGGCAGTGGCGCTCTGGCTACAGGCAA
CCCCCGATCATCTGGCAGCACTCAGCTCGCTGAAACTCGTGCAGGTGGGTGGCGCAAGCTTTGCGGAGGCGCTTGCCCGG
CAGGTGCCACAGGTTCTGGGCTGCAAGCTGCAACAGGTCTTCGGCATGGCCGAGGGGCTGGTCAACTATACCCGCCTTGA
CGACCCGGACGAGATCGTCTTCACCACCCAGGGCCGTCCGATCAGCCCGGACGATGAAATCAGGATCGTCGATGAGGATG
GCGAACCCGTGGCCGAAGGCCAGCCAGGAATGCTGGCCACGCGCGGGCCTTACACGTTCTGCGGCTATTATCGCAACCCC
GAACATAATGCGCAGGTCTTCGACAGTGAAGGCTTTTATTATTCGGGCGACGTGGTCCAGCGCACGCCGGAAGGCTATCT
GCGCGTCGTGGGCCGCGTGAAGGACCAGATTAATCGCGGCGGCGAGAAAGTTGCCGCCGAGGAGATTGAAAACCTGATCC
TCCTCCATCCCGATGTGACGCACGCCGCACTTGTCGCCATGCAGGATGAATTGCTGGGCGAGAAAAGCTGCGCTTTCATC
GTCTCGCGCAATCCCGATCTCAAGCCGCCTGTTCTGCGGCGTCACCTGCTGGCGCTGGGCGTTGCCGAATACAAGCTTCC
AGACCGCATCAGGCTCATTGAAACCATGCCGCTCACCGCAGTTGGCAAGATCGACAAAAAGCATCTGCGCAAGCTTTCAA
GCCCATCCTCCCCCGGTTAG

Upstream 100 bases:

>100_bases
GCCTCTCCATGTCTTTGTTTCTACGCATTATCCGACGCAGAGCCGCTTCGCATTGCGGGCTCGAAAACTGCACTCGTTTT
TATTTCAAGCAGAACAAGTC

Downstream 100 bases:

>100_bases
CTCGCGTTTTACTCCAGTCGGATTACGCTTCAAACCCACAAAGGCAAGACCATGGCCATTCCCAAAATCGCCGATTATCC
AATGCCGCAAAAGGCAGGCT

Product: enterobactin synthase subunit E

Products: NA

Alternate protein names: Enterochelin synthase E; 2,3-dihydroxybenzoate-AMP ligase; Dihydroxybenzoic acid-activating enzyme; S-dihydroxybenzoyltransferase [H]

Number of amino acids: Translated: 539; Mature: 538

Protein sequence:

>539_residues
MTIEFTHWPPARERLYREKGYWIDKPLTRALEEQAAMRPDAPAILCGERRFTYAELDRLSSNLASRLAAAGIGKGDTALV
QLPNIAEFYIVFFALMKAGIAPVNALFSHRKLELGAYASQIEPRLLIGSRQHELFMDDAFARDLGKNLSAPLLTLFAGEA
DPASSLDHWIATPADKAVPFSPTGAGEVAFFQLSGGSTGTPKLIPRTHNDYDYSVRASAEICALTPQTRFLCALPTAHNY
PMSSPGALGVFHAGGCVVMASNPEPLNCFSIIERHGVNMVALVPPAVALWLQATPDHLAALSSLKLVQVGGASFAEALAR
QVPQVLGCKLQQVFGMAEGLVNYTRLDDPDEIVFTTQGRPISPDDEIRIVDEDGEPVAEGQPGMLATRGPYTFCGYYRNP
EHNAQVFDSEGFYYSGDVVQRTPEGYLRVVGRVKDQINRGGEKVAAEEIENLILLHPDVTHAALVAMQDELLGEKSCAFI
VSRNPDLKPPVLRRHLLALGVAEYKLPDRIRLIETMPLTAVGKIDKKHLRKLSSPSSPG

Sequences:

>Translated_539_residues
MTIEFTHWPPARERLYREKGYWIDKPLTRALEEQAAMRPDAPAILCGERRFTYAELDRLSSNLASRLAAAGIGKGDTALV
QLPNIAEFYIVFFALMKAGIAPVNALFSHRKLELGAYASQIEPRLLIGSRQHELFMDDAFARDLGKNLSAPLLTLFAGEA
DPASSLDHWIATPADKAVPFSPTGAGEVAFFQLSGGSTGTPKLIPRTHNDYDYSVRASAEICALTPQTRFLCALPTAHNY
PMSSPGALGVFHAGGCVVMASNPEPLNCFSIIERHGVNMVALVPPAVALWLQATPDHLAALSSLKLVQVGGASFAEALAR
QVPQVLGCKLQQVFGMAEGLVNYTRLDDPDEIVFTTQGRPISPDDEIRIVDEDGEPVAEGQPGMLATRGPYTFCGYYRNP
EHNAQVFDSEGFYYSGDVVQRTPEGYLRVVGRVKDQINRGGEKVAAEEIENLILLHPDVTHAALVAMQDELLGEKSCAFI
VSRNPDLKPPVLRRHLLALGVAEYKLPDRIRLIETMPLTAVGKIDKKHLRKLSSPSSPG
>Mature_538_residues
TIEFTHWPPARERLYREKGYWIDKPLTRALEEQAAMRPDAPAILCGERRFTYAELDRLSSNLASRLAAAGIGKGDTALVQ
LPNIAEFYIVFFALMKAGIAPVNALFSHRKLELGAYASQIEPRLLIGSRQHELFMDDAFARDLGKNLSAPLLTLFAGEAD
PASSLDHWIATPADKAVPFSPTGAGEVAFFQLSGGSTGTPKLIPRTHNDYDYSVRASAEICALTPQTRFLCALPTAHNYP
MSSPGALGVFHAGGCVVMASNPEPLNCFSIIERHGVNMVALVPPAVALWLQATPDHLAALSSLKLVQVGGASFAEALARQ
VPQVLGCKLQQVFGMAEGLVNYTRLDDPDEIVFTTQGRPISPDDEIRIVDEDGEPVAEGQPGMLATRGPYTFCGYYRNPE
HNAQVFDSEGFYYSGDVVQRTPEGYLRVVGRVKDQINRGGEKVAAEEIENLILLHPDVTHAALVAMQDELLGEKSCAFIV
SRNPDLKPPVLRRHLLALGVAEYKLPDRIRLIETMPLTAVGKIDKKHLRKLSSPSSPG

Specific function: Activates the carboxylate group of 2,3-dihydroxy- benzoate (2,3-DHB), via ATP-dependent PPi exchange reactions, to the acyladenylate. Then, catalyzes the acylation of holo-EntB with 2,3-DHB adenylate, preparing that molecule for amide bond formation with

COG id: COG1021

COG function: function code Q; Peptide arylation enzymes

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. EntE subfamily [H]

Homologues:

Organism=Homo sapiens, GI38505220, Length=525, Percent_Identity=24, Blast_Score=112, Evalue=8e-25,
Organism=Homo sapiens, GI156151445, Length=554, Percent_Identity=23.826714801444, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI42544132, Length=542, Percent_Identity=23.0627306273063, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI122937307, Length=534, Percent_Identity=24.5318352059925, Blast_Score=102, Evalue=9e-22,
Organism=Homo sapiens, GI58082049, Length=538, Percent_Identity=23.7918215613383, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI157311624, Length=533, Percent_Identity=23.4521575984991, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI157311622, Length=533, Percent_Identity=23.4521575984991, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI115511026, Length=532, Percent_Identity=24.4360902255639, Blast_Score=100, Evalue=6e-21,
Organism=Homo sapiens, GI187761345, Length=543, Percent_Identity=24.1252302025783, Blast_Score=94, Evalue=5e-19,
Organism=Homo sapiens, GI187761343, Length=543, Percent_Identity=24.1252302025783, Blast_Score=94, Evalue=5e-19,
Organism=Homo sapiens, GI28416953, Length=532, Percent_Identity=25, Blast_Score=94, Evalue=5e-19,
Organism=Homo sapiens, GI57165410, Length=175, Percent_Identity=28, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI57165412, Length=175, Percent_Identity=28, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1786810, Length=536, Percent_Identity=57.6492537313433, Blast_Score=611, Evalue=1e-176,
Organism=Escherichia coli, GI145693145, Length=546, Percent_Identity=32.0512820512821, Blast_Score=226, Evalue=4e-60,
Organism=Escherichia coli, GI1788107, Length=540, Percent_Identity=24.8148148148148, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI221142682, Length=490, Percent_Identity=24.2857142857143, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1790505, Length=532, Percent_Identity=22.3684210526316, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI1786529, Length=563, Percent_Identity=23.0905861456483, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1786801, Length=461, Percent_Identity=25.1626898047722, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1789201, Length=355, Percent_Identity=25.6338028169014, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1788595, Length=446, Percent_Identity=23.0941704035874, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17557194, Length=542, Percent_Identity=27.6752767527675, Blast_Score=128, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI17538037, Length=541, Percent_Identity=25.8780036968577, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17560308, Length=529, Percent_Identity=25.5198487712665, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17559526, Length=562, Percent_Identity=25.2669039145907, Blast_Score=108, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17531443, Length=539, Percent_Identity=23.0055658627087, Blast_Score=108, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI32563687, Length=511, Percent_Identity=22.5048923679061, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71996755, Length=514, Percent_Identity=24.9027237354086, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI71994694, Length=524, Percent_Identity=23.2824427480916, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71994703, Length=525, Percent_Identity=23.2380952380952, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71994690, Length=528, Percent_Identity=23.6742424242424, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17558820, Length=520, Percent_Identity=21.7307692307692, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71985884, Length=400, Percent_Identity=23.75, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17560140, Length=286, Percent_Identity=25.8741258741259, Blast_Score=86, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI71983001, Length=385, Percent_Identity=24.9350649350649, Blast_Score=73, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI71982997, Length=385, Percent_Identity=24.9350649350649, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17510401, Length=151, Percent_Identity=28.476821192053, Blast_Score=65, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6319699, Length=359, Percent_Identity=24.7910863509749, Blast_Score=116, Evalue=9e-27,
Organism=Saccharomyces cerevisiae, GI6323182, Length=606, Percent_Identity=23.4323432343234, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6319264, Length=540, Percent_Identity=24.0740740740741, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6323903, Length=174, Percent_Identity=24.1379310344828, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24581924, Length=534, Percent_Identity=23.9700374531835, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI18859661, Length=512, Percent_Identity=24.21875, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI19922652, Length=504, Percent_Identity=22.4206349206349, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI21358303, Length=298, Percent_Identity=25.1677852348993, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI281365686, Length=299, Percent_Identity=27.0903010033445, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24648260, Length=275, Percent_Identity=24.7272727272727, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21356947, Length=399, Percent_Identity=24.0601503759398, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21355181, Length=498, Percent_Identity=22.4899598393574, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24653035, Length=288, Percent_Identity=25.6944444444444, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24656500, Length=498, Percent_Identity=23.8955823293173, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21356441, Length=462, Percent_Identity=22.9437229437229, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24666501, Length=359, Percent_Identity=26.7409470752089, Blast_Score=76, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24666497, Length=359, Percent_Identity=26.7409470752089, Blast_Score=76, Evalue=7e-14,
Organism=Drosophila melanogaster, GI62472339, Length=547, Percent_Identity=23.5831809872029, Blast_Score=76, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24667955, Length=573, Percent_Identity=23.2111692844677, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI281366413, Length=359, Percent_Identity=26.7409470752089, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI161076582, Length=245, Percent_Identity=26.1224489795918, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24648257, Length=273, Percent_Identity=24.5421245421245, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24667959, Length=379, Percent_Identity=24.802110817942, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR011963 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =2.7.7.58 [H]

Molecular weight: Translated: 58804; Mature: 58673

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00605 ATPASE_C ; PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIEFTHWPPARERLYREKGYWIDKPLTRALEEQAAMRPDAPAILCGERRFTYAELDRLS
CEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHH
SNLASRLAAAGIGKGDTALVQLPNIAEFYIVFFALMKAGIAPVNALFSHRKLELGAYASQ
HHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHCCCEEHHHHHHH
IEPRLLIGSRQHELFMDDAFARDLGKNLSAPLLTLFAGEADPASSLDHWIATPADKAVPF
CCCEEEECCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCC
SPTGAGEVAFFQLSGGSTGTPKLIPRTHNDYDYSVRASAEICALTPQTRFLCALPTAHNY
CCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCEEEEECCCCCEEEECCCCCCC
PMSSPGALGVFHAGGCVVMASNPEPLNCFSIIERHGVNMVALVPPAVALWLQATPDHLAA
CCCCCCCEEEEECCCEEEEECCCCCCHHHHHHHHCCCCEEEECCCCEEEEEECCHHHHHH
LSSLKLVQVGGASFAEALARQVPQVLGCKLQQVFGMAEGLVNYTRLDDPDEIVFTTQGRP
HHHCEEEEECCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCC
ISPDDEIRIVDEDGEPVAEGQPGMLATRGPYTFCGYYRNPEHNAQVFDSEGFYYSGDVVQ
CCCCCCEEEECCCCCCCCCCCCCEEEECCCCEEEEECCCCCCCCEEEECCCCEEECCHHC
RTPEGYLRVVGRVKDQINRGGEKVAAEEIENLILLHPDVTHAALVAMQDELLGEKSCAFI
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCEEEECCCHHHHHHHHHHHHHCCCCCEEEE
VSRNPDLKPPVLRRHLLALGVAEYKLPDRIRLIETMPLTAVGKIDKKHLRKLSSPSSPG
EECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHCCCCHHHHHHHCCCCCCC
>Mature Secondary Structure 
TIEFTHWPPARERLYREKGYWIDKPLTRALEEQAAMRPDAPAILCGERRFTYAELDRLS
EEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHH
SNLASRLAAAGIGKGDTALVQLPNIAEFYIVFFALMKAGIAPVNALFSHRKLELGAYASQ
HHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHCCCEEHHHHHHH
IEPRLLIGSRQHELFMDDAFARDLGKNLSAPLLTLFAGEADPASSLDHWIATPADKAVPF
CCCEEEECCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCC
SPTGAGEVAFFQLSGGSTGTPKLIPRTHNDYDYSVRASAEICALTPQTRFLCALPTAHNY
CCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCEEEEECCCCCEEEECCCCCCC
PMSSPGALGVFHAGGCVVMASNPEPLNCFSIIERHGVNMVALVPPAVALWLQATPDHLAA
CCCCCCCEEEEECCCEEEEECCCCCCHHHHHHHHCCCCEEEECCCCEEEEEECCHHHHHH
LSSLKLVQVGGASFAEALARQVPQVLGCKLQQVFGMAEGLVNYTRLDDPDEIVFTTQGRP
HHHCEEEEECCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCC
ISPDDEIRIVDEDGEPVAEGQPGMLATRGPYTFCGYYRNPEHNAQVFDSEGFYYSGDVVQ
CCCCCCEEEECCCCCCCCCCCCCEEEECCCCEEEEECCCCCCCCEEEECCCCEEECCHHC
RTPEGYLRVVGRVKDQINRGGEKVAAEEIENLILLHPDVTHAALVAMQDELLGEKSCAFI
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCEEEECCCHHHHHHHHHHHHHCCCCCEEEE
VSRNPDLKPPVLRRHLLALGVAEYKLPDRIRLIETMPLTAVGKIDKKHLRKLSSPSSPG
EECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHCCCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]