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The map label for this gene is entD [H]

Identifier: 163844211

GI number: 163844211

Start: 9669

End: 10409

Strand: Reverse

Name: entD [H]

Synonym: BSUIS_B0014

Alternate gene names: 163844211

Gene position: 10409-9669 (Counterclockwise)

Preceding gene: 163844212

Following gene: 163844209

Centisome position: 0.74

GC content: 62.75

Gene sequence:

>741_bases
ATGGCGGAGCAACCCTTGGCGCATGAGACCGCGTGGCTTGCGCCAGGCGCCCACGGCGTTTCGGTCATAACGCCGTGGGC
CGATCTGGCCGCAGACTGCTGTTTCGTCATGACCCGCTATTCGGCCAGCAAACCCTTGATAGACCCTTCTATCCCCGCTC
CTGCGCGCGCCGCCCCGCGCCGAAAGGCGGAATTTGTCGCCGGGCAAGCCTGCGCGCGCGAAGCCCTGCGCCTTGCAGGC
GGGCCTGCCGTAATGCCGGGGCGTGGGGATGACCATGCGCCCATCTGGCCACCGGGCATGGTCGGTTCCATCTCCCATAG
CAAGGGATGCGCAATTGCCGTCGCCGCCCGCCAGAGCCGCTATCTGGGCCTCGGCATTGATATCGAACGCCTGCAAACGG
AAAGTTCGGCCCGAAAAATCGCCCCGCAGATACTGGGTGAAAGCGAGCTGCGCCAATTGCCCGAAGCCGGCCGGGCCTTT
CTGGTGAGCCTTGCTTTTTCTGCAAAAGAAAGCCTGTTCAAGGCGCTTTATCCGGCAGTGCAGCAATTCCAGAGTTTCCA
TGCCGCAGCCTTTTCCTTTGACGGCGACAGGCAGCAAGCCTGCCTTGAGCTTGCGGTGGACTGGTCGGCGCAATGGCGCC
GCGGGCAGCGGTTCCCGATCCGCTTTTTCTGCCGCGACGGTTTCATTCTCACCTGCACGGCCATTGCGGCCATCAGTTGC
CGTTCAGAAATGTCAGGATAA

Upstream 100 bases:

>100_bases
CCGCTCGGCAAGATCGCGATGCCGGACGATATCGCCAATGTCATCCTGTTTCTGGCGTCCGATCAGGCCGGGCATGTGAC
GATGCAGGATATTGTCGTGG

Downstream 100 bases:

>100_bases
GTTGCTGGACATTGCCGCCATTGCCCATTTCGACATGATCGAAATCCCGGCCCTGTGCGCGCTGAACCTGAACCATATCC
GTAATCTCCTTCGCCAGAAC

Product: hypothetical protein

Products: pyrophosphate; AMP; enterobactin; aryl carrier protein/isochorismatase; 3',5'-ADP; serine activating enzyme + [C]

Alternate protein names: Enterobactin synthase component D; Enterochelin synthase D [H]

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MAEQPLAHETAWLAPGAHGVSVITPWADLAADCCFVMTRYSASKPLIDPSIPAPARAAPRRKAEFVAGQACAREALRLAG
GPAVMPGRGDDHAPIWPPGMVGSISHSKGCAIAVAARQSRYLGLGIDIERLQTESSARKIAPQILGESELRQLPEAGRAF
LVSLAFSAKESLFKALYPAVQQFQSFHAAAFSFDGDRQQACLELAVDWSAQWRRGQRFPIRFFCRDGFILTCTAIAAISC
RSEMSG

Sequences:

>Translated_246_residues
MAEQPLAHETAWLAPGAHGVSVITPWADLAADCCFVMTRYSASKPLIDPSIPAPARAAPRRKAEFVAGQACAREALRLAG
GPAVMPGRGDDHAPIWPPGMVGSISHSKGCAIAVAARQSRYLGLGIDIERLQTESSARKIAPQILGESELRQLPEAGRAF
LVSLAFSAKESLFKALYPAVQQFQSFHAAAFSFDGDRQQACLELAVDWSAQWRRGQRFPIRFFCRDGFILTCTAIAAISC
RSEMSG
>Mature_245_residues
AEQPLAHETAWLAPGAHGVSVITPWADLAADCCFVMTRYSASKPLIDPSIPAPARAAPRRKAEFVAGQACAREALRLAGG
PAVMPGRGDDHAPIWPPGMVGSISHSKGCAIAVAARQSRYLGLGIDIERLQTESSARKIAPQILGESELRQLPEAGRAFL
VSLAFSAKESLFKALYPAVQQFQSFHAAAFSFDGDRQQACLELAVDWSAQWRRGQRFPIRFFCRDGFILTCTAIAAISCR
SEMSG

Specific function: Catalyzes the transfer of the 4'-phosphopantetheine moiety from coenzyme A to apo-domains of both entB (an ArCP domain) and entF (a PCP domain). Plays an essential role in the assembly of the enterobactin [H]

COG id: COG2977

COG function: function code Q; Phosphopantetheinyl transferase component of siderophore synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P-Pant transferase superfamily. EntD family [H]

Homologues:

Organism=Escherichia coli, GI1786797, Length=129, Percent_Identity=40.3100775193798, Blast_Score=79, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008278
- InterPro:   IPR003542 [H]

Pfam domain/function: PF01648 ACPS [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 26515; Mature: 26383

Theoretical pI: Translated: 8.41; Mature: 8.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEQPLAHETAWLAPGAHGVSVITPWADLAADCCFVMTRYSASKPLIDPSIPAPARAAPR
CCCCCCCCCHHCCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
RKAEFVAGQACAREALRLAGGPAVMPGRGDDHAPIWPPGMVGSISHSKGCAIAVAARQSR
HHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCC
YLGLGIDIERLQTESSARKIAPQILGESELRQLPEAGRAFLVSLAFSAKESLFKALYPAV
EEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QQFQSFHAAAFSFDGDRQQACLELAVDWSAQWRRGQRFPIRFFCRDGFILTCTAIAAISC
HHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHCCCCCCCEEEEECCCEEEHHHHHHHHHH
RSEMSG
HHHCCC
>Mature Secondary Structure 
AEQPLAHETAWLAPGAHGVSVITPWADLAADCCFVMTRYSASKPLIDPSIPAPARAAPR
CCCCCCCCHHCCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
RKAEFVAGQACAREALRLAGGPAVMPGRGDDHAPIWPPGMVGSISHSKGCAIAVAARQSR
HHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCC
YLGLGIDIERLQTESSARKIAPQILGESELRQLPEAGRAFLVSLAFSAKESLFKALYPAV
EEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QQFQSFHAAAFSFDGDRQQACLELAVDWSAQWRRGQRFPIRFFCRDGFILTCTAIAAISC
HHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHCCCCCCCEEEEECCCEEEHHHHHHHHHH
RSEMSG
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 6 ATP; L-serine; 2,3-dihydroxybenzoate; Coenzyme A; apo-EntB; apo-serine activating enzyme [C]

Specific reaction: 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin Coenzyme A + apo-EntB = aryl carrier protein/isochorismatase + 3',5'-ADP Coenzyme A + apo-serine activating enzyme = 3',5'-ADP + serine activating enzyme [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]