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The map label for this gene is lysS [H]

Identifier: 162448223

GI number: 162448223

Start: 1449416

End: 1450903

Strand: Direct

Name: lysS [H]

Synonym: ACL_1388

Alternate gene names: 162448223

Gene position: 1449416-1450903 (Clockwise)

Preceding gene: 162448222

Following gene: 162448256

Centisome position: 96.82

GC content: 34.74

Gene sequence:

>1488_bases
ATGTTTCCTGATGATTTAAATGAACAACAAAAAATAAGACTTCAAAAAGTTGAAGACTTAAGAAAATTAGGTGTTGATCC
TTTCGGACACCGTTTTGACCGCACACATTTATCTAATGAGATTCATACTACATATGGTAGTTTAACCCGCGAGGAATTAG
AAGCTAAAGAAGTACATGTTATTATTGCAGGTCGTATTGTCTTAAGACGTACCCAAGGTAAAGCTGGTTTTATCCAACTA
CAAGATAAAGAAGGCCGTATTCAAGTATATGTACGCCTAGATGCAGTTGGTGAATTCCAATTCGAATTATTTAAACACTC
CGATTTAGGTGATATCGTTGGTATTAAAGGTGTTTTATTTAGAACTAAAACAGATGAACTTACTGTTAAAGCAAGTGAAT
TTGTTCACTTAACTAAAGCCATTACACCACTTCCTGATAAATTCCATGGTCTACAAGACAAGGAAGAAGCAAGACGTCGC
CGTTATGTAGATTTAATTGTTAACGAAGATTCTATGCGTGTTGCAAGAACACGTCCAAGAATTATTCGTGCATTTCAACA
CTATTTTGATTCTAACGGTTTTATTGAAGTTGAAACACCAGTACTACATCCAATCTTAGGTGGTGCTGCTGCTAGACCGT
TTGTTACACACCATAACACACTGGATATGGACTTCTACTTACGTATTGCTACAGAGTTACCGCTAAAAAGATTAATTGTT
GGTGGTATGGAACGTGTTTACGAAATTGGTCGTCTATTTAGAAACGAAGGCATTAGTGCTAAACATAACCCAGAGTTTAC
AACTGTTGAAGCTTACCAAGCATTTGGTGATGTTACGGATATGATGAAACTTGTTGAAGACTGTTTATCTAGTGTAGCTT
TTGAAGTAAATGGTTCATATGATGTCGTATTTAATGATCAAACCATTCATTTAAAAGACTTTAAACGTGCACATATGGTC
GATTTAATTAAAGAACAAACAGGTGAAGACTTCTTTAATCAATATAGTCTAGAAGAAGCTAAACAAATTGCTAAAAAGCA
TAACGTACAAGTGGAACCTCACTTTACAGTAGGACACATCATTGAAGCATTCTTTAATGACTATGTTGAAAAAACAATCA
TCCAACCTACAATCGTATTTGGACATCCAGTAGAAATCTCACCACTGGCAAAATCAAATGATAAAGACCCTAGATTTACA
GATCGTTTTGAATTATTTATCATGGGTTCTGAATATGCAAATGCATTTTCAGAGTTAAATGATCCAGTGGATCAAAAAAA
TAGATTCTTAGACCAATTAAAACAAAAAGAAAAAGGCGATGACGAAGCATCCGAAATGGATATTGACTTTGTTGAGGCAC
TTGAATATGGTATGCCTCCAACCGGTGGTATTGGTATTGGTATTGATAGATTCATTATGCTCCTTACAAATACACCCAAT
ATTCGTGATGTTATCTTATTTCCTCACGCTAGAATTAAAACTAAATAA

Upstream 100 bases:

>100_bases
CAAGACATGTATACACTCATTTCCGAAGAAAAACGGTCTTTATGATCGTTTTTTAATGGAAATTATAATATAATAAGTAA
GAATAAGGAGGAAATCAAAT

Downstream 100 bases:

>100_bases
TATATCAAAAAACCTCCAATATTTTGGAGGTTTTTATTTGCATGACGTATTAAATTAAGATATTTGTCCAATAGTTTCCG
CGAGCATATAAATAAACATG

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MFPDDLNEQQKIRLQKVEDLRKLGVDPFGHRFDRTHLSNEIHTTYGSLTREELEAKEVHVIIAGRIVLRRTQGKAGFIQL
QDKEGRIQVYVRLDAVGEFQFELFKHSDLGDIVGIKGVLFRTKTDELTVKASEFVHLTKAITPLPDKFHGLQDKEEARRR
RYVDLIVNEDSMRVARTRPRIIRAFQHYFDSNGFIEVETPVLHPILGGAAARPFVTHHNTLDMDFYLRIATELPLKRLIV
GGMERVYEIGRLFRNEGISAKHNPEFTTVEAYQAFGDVTDMMKLVEDCLSSVAFEVNGSYDVVFNDQTIHLKDFKRAHMV
DLIKEQTGEDFFNQYSLEEAKQIAKKHNVQVEPHFTVGHIIEAFFNDYVEKTIIQPTIVFGHPVEISPLAKSNDKDPRFT
DRFELFIMGSEYANAFSELNDPVDQKNRFLDQLKQKEKGDDEASEMDIDFVEALEYGMPPTGGIGIGIDRFIMLLTNTPN
IRDVILFPHARIKTK

Sequences:

>Translated_495_residues
MFPDDLNEQQKIRLQKVEDLRKLGVDPFGHRFDRTHLSNEIHTTYGSLTREELEAKEVHVIIAGRIVLRRTQGKAGFIQL
QDKEGRIQVYVRLDAVGEFQFELFKHSDLGDIVGIKGVLFRTKTDELTVKASEFVHLTKAITPLPDKFHGLQDKEEARRR
RYVDLIVNEDSMRVARTRPRIIRAFQHYFDSNGFIEVETPVLHPILGGAAARPFVTHHNTLDMDFYLRIATELPLKRLIV
GGMERVYEIGRLFRNEGISAKHNPEFTTVEAYQAFGDVTDMMKLVEDCLSSVAFEVNGSYDVVFNDQTIHLKDFKRAHMV
DLIKEQTGEDFFNQYSLEEAKQIAKKHNVQVEPHFTVGHIIEAFFNDYVEKTIIQPTIVFGHPVEISPLAKSNDKDPRFT
DRFELFIMGSEYANAFSELNDPVDQKNRFLDQLKQKEKGDDEASEMDIDFVEALEYGMPPTGGIGIGIDRFIMLLTNTPN
IRDVILFPHARIKTK
>Mature_495_residues
MFPDDLNEQQKIRLQKVEDLRKLGVDPFGHRFDRTHLSNEIHTTYGSLTREELEAKEVHVIIAGRIVLRRTQGKAGFIQL
QDKEGRIQVYVRLDAVGEFQFELFKHSDLGDIVGIKGVLFRTKTDELTVKASEFVHLTKAITPLPDKFHGLQDKEEARRR
RYVDLIVNEDSMRVARTRPRIIRAFQHYFDSNGFIEVETPVLHPILGGAAARPFVTHHNTLDMDFYLRIATELPLKRLIV
GGMERVYEIGRLFRNEGISAKHNPEFTTVEAYQAFGDVTDMMKLVEDCLSSVAFEVNGSYDVVFNDQTIHLKDFKRAHMV
DLIKEQTGEDFFNQYSLEEAKQIAKKHNVQVEPHFTVGHIIEAFFNDYVEKTIIQPTIVFGHPVEISPLAKSNDKDPRFT
DRFELFIMGSEYANAFSELNDPVDQKNRFLDQLKQKEKGDDEASEMDIDFVEALEYGMPPTGGIGIGIDRFIMLLTNTPN
IRDVILFPHARIKTK

Specific function: Unknown

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=510, Percent_Identity=38.0392156862745, Blast_Score=330, Evalue=1e-90,
Organism=Homo sapiens, GI194272210, Length=510, Percent_Identity=38.0392156862745, Blast_Score=330, Evalue=2e-90,
Organism=Homo sapiens, GI40789249, Length=244, Percent_Identity=25.8196721311475, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI1789256, Length=490, Percent_Identity=49.5918367346939, Blast_Score=471, Evalue=1e-134,
Organism=Escherichia coli, GI1790571, Length=490, Percent_Identity=48.1632653061224, Blast_Score=457, Evalue=1e-130,
Organism=Escherichia coli, GI87082379, Length=320, Percent_Identity=28.75, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1788173, Length=252, Percent_Identity=28.968253968254, Blast_Score=79, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17535925, Length=507, Percent_Identity=42.0118343195266, Blast_Score=376, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17535927, Length=507, Percent_Identity=42.0118343195266, Blast_Score=376, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI71994340, Length=498, Percent_Identity=42.1686746987952, Blast_Score=372, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI32566633, Length=273, Percent_Identity=24.5421245421245, Blast_Score=73, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6320242, Length=512, Percent_Identity=41.015625, Blast_Score=350, Evalue=2e-97,
Organism=Saccharomyces cerevisiae, GI6324256, Length=382, Percent_Identity=32.9842931937173, Blast_Score=188, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6323011, Length=469, Percent_Identity=24.7334754797441, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24640851, Length=501, Percent_Identity=39.1217564870259, Blast_Score=348, Evalue=7e-96,
Organism=Drosophila melanogaster, GI24640849, Length=501, Percent_Identity=39.1217564870259, Blast_Score=347, Evalue=9e-96,
Organism=Drosophila melanogaster, GI24584738, Length=274, Percent_Identity=27.007299270073, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 56927; Mature: 56927

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFPDDLNEQQKIRLQKVEDLRKLGVDPFGHRFDRTHLSNEIHTTYGSLTREELEAKEVHV
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHCCCEEEE
IIAGRIVLRRTQGKAGFIQLQDKEGRIQVYVRLDAVGEFQFELFKHSDLGDIVGIKGVLF
EEEEEEEEEECCCCCCEEEEECCCCEEEEEEEEECCCCEEHHHHCCCCCCCHHCCCEEEE
RTKTDELTVKASEFVHLTKAITPLPDKFHGLQDKEEARRRRYVDLIVNEDSMRVARTRPR
EECCCCEEEEHHHHHHHHHHHCCCCHHHCCCCCHHHHHHCEEEEEEECCCCHHHHHHHHH
IIRAFQHYFDSNGFIEVETPVLHPILGGAAARPFVTHHNTLDMDFYLRIATELPLKRLIV
HHHHHHHHHCCCCEEEECCCHHHHHHCCCCCCCEEECCCCCCCEEEEEEHHHCCHHHHHH
GGMERVYEIGRLFRNEGISAKHNPEFTTVEAYQAFGDVTDMMKLVEDCLSSVAFEVNGSY
HHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCE
DVVFNDQTIHLKDFKRAHMVDLIKEQTGEDFFNQYSLEEAKQIAKKHNVQVEPHFTVGHI
EEEECCCEEEHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHCCCEECCCCHHHHH
IEAFFNDYVEKTIIQPTIVFGHPVEISPLAKSNDKDPRFTDRFELFIMGSEYANAFSELN
HHHHHHHHHHHHHCCCEEEECCCCEECCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHC
DPVDQKNRFLDQLKQKEKGDDEASEMDIDFVEALEYGMPPTGGIGIGIDRFIMLLTNTPN
CCHHHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHCCCCCCCCCCCHHHEEEEEECCCC
IRDVILFPHARIKTK
CEEEEEECCCCCCCC
>Mature Secondary Structure
MFPDDLNEQQKIRLQKVEDLRKLGVDPFGHRFDRTHLSNEIHTTYGSLTREELEAKEVHV
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHCCCEEEE
IIAGRIVLRRTQGKAGFIQLQDKEGRIQVYVRLDAVGEFQFELFKHSDLGDIVGIKGVLF
EEEEEEEEEECCCCCCEEEEECCCCEEEEEEEEECCCCEEHHHHCCCCCCCHHCCCEEEE
RTKTDELTVKASEFVHLTKAITPLPDKFHGLQDKEEARRRRYVDLIVNEDSMRVARTRPR
EECCCCEEEEHHHHHHHHHHHCCCCHHHCCCCCHHHHHHCEEEEEEECCCCHHHHHHHHH
IIRAFQHYFDSNGFIEVETPVLHPILGGAAARPFVTHHNTLDMDFYLRIATELPLKRLIV
HHHHHHHHHCCCCEEEECCCHHHHHHCCCCCCCEEECCCCCCCEEEEEEHHHCCHHHHHH
GGMERVYEIGRLFRNEGISAKHNPEFTTVEAYQAFGDVTDMMKLVEDCLSSVAFEVNGSY
HHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCE
DVVFNDQTIHLKDFKRAHMVDLIKEQTGEDFFNQYSLEEAKQIAKKHNVQVEPHFTVGHI
EEEECCCEEEHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHCCCEECCCCHHHHH
IEAFFNDYVEKTIIQPTIVFGHPVEISPLAKSNDKDPRFTDRFELFIMGSEYANAFSELN
HHHHHHHHHHHHHCCCEEEECCCCEECCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHC
DPVDQKNRFLDQLKQKEKGDDEASEMDIDFVEALEYGMPPTGGIGIGIDRFIMLLTNTPN
CCHHHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHCCCCCCCCCCCHHHEEEEEECCCC
IRDVILFPHARIKTK
CEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA