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The map label for this gene is clpB [H]

Identifier: 162447977

GI number: 162447977

Start: 1186761

End: 1188887

Strand: Direct

Name: clpB [H]

Synonym: ACL_1124

Alternate gene names: 162447977

Gene position: 1186761-1188887 (Clockwise)

Preceding gene: 162447975

Following gene: 162447979

Centisome position: 79.28

GC content: 33.47

Gene sequence:

>2127_bases
ATGCAATTTAACCCAATGGATGATTATTCAAAAGATCCTGACATTTTAAAAAAGTTCGGTAGAAATATTGTAGAACTTGT
TAAAGAAGGTAAAGTCGACCCAGTGATTGGTCGTGAGGAAGAGATTAGACGTATTATTAAAATTCTATCTAGAAAAACCA
AAAACAACCCTGTTTTAATTGGTGAACCGGGGGTGGGTAAAACAGCGATTGTTGAAGGCTTAGCTAGAAGAATTGTTGAT
AAAGACGTACCTTTAAGTCTGCAAAATAAAATTGTTTATGAACTAGACTTAGCTCAATTAGTTGCTGGTGCAAAATTTAG
AGGTGAGTTTGAAGAACGTTTGAAGGATGTTTTAAATAAGATTAAAGAAAGCGATGGCCAAATCATCTTATTTATTGATG
AATTACATAATATTGTAGGTGCTGGTCGTGCAGATGGTGCAATGGATGCCTCAAACATCTTAAAACCAATGCTTGCACGT
GGTGAACTTCACTGTATTGGTGCTACAACATTAAATGAATACCGCTTATATATTGAAAAAGATAGTGCACTCGAAAGAAG
ATTTCAAAAGATCACTGTGACTGAACCAACTATGGAAGATACAATTTCTATCTTACGTGGACTTAAAGATCGATTTGAAG
CACATCATGGTGTGCACATCTCTGATGGTGCCATCATCGCTGCTACAAATCTATCAGCACGTTACATTACAGATAGATTC
TTACCAGATAAAGCAATCGACCTTATTGATGAGGCTTGTGCTTCTATTCGTATGGAAATTGATTCTATGCCAGTGGAACT
TGATGATGTTCAAAGAAAAATACTACAACTAGAAATTGAAAAATCTGCTTTAAGTAAAGAAACAGATACATTAAGTAAAG
AGCGTCTATCTAAAATTAAATCTGAGATTGATGATTTAAAATTAGAGGAAGCCAGTCTAAAAAGTAGATGGGAAAAAGAA
AAATCTCAACTTAATTTATCAAAACGCAAAAAAGAAACCTTGGAACAACTTAAAAACGAACTTAATACTGCTTATAACGA
AGGTAACTATTCTAAGGCTGCAGAACTTCAATACTCAAAAATACCTAATCTAGAAAAAGAAATATCTGAATTATCAGATA
CTTCAACAGATGATAAACTTCTAACAGAAACTGTTACCCCTGAATCGATTGCTGAAATCGTAAGTAAATGGACCCATATA
CCTGTAACAAAACTAGTATCAGGTGATAGAGAAAAACTTAGAAACCTACGTTTAAGCCTAGGTAAACGTGTATTAGGTCA
AGATCATGCACTAGAACTGATTAGTAACGCCATATTACGTCATCGTGCAGGTATCAAAGATCCAAATAGACCAATTGGTT
CATTCTTATTCTTAGGTCCGACTGGTGTTGGTAAAACAGAGGCTGCTAAAGCACTTGCTGAAAATTTATTTGACTCAGAA
AATCATATTGTTCGTATAGATATGTCAGAGTATATGGAATCACATAGTGTCTCTCGTTTAATCGGCGCACCTCCAGGATA
TGTAGGTTATGATCAAGGCGGTCAACTTACAGAAAAAATAAGACGTATGCCTTACGCAATCGTCTTATTTGATGAAATTG
AAAAGGCACATCCTGAAGTCTTTAATATCCTACTTCAAGTATTAGATGATGGTCACTTAACAGATGGACAAGGTCGTACA
ATAGATTTTAAAAATACTGTCATTATTATGACATCTAACATTGGCAGTCAATATTTACTAGAAAACGCAAGTGATGCCCA
AGAAAAAATAGATAAACTGCTAAAACAATCCTTTAAACCAGAATTTTTAAATAGAATTGATGAAATTATTACATTTAACT
CACTTGGATTTAAAGTTCAAGTGGATATCACAAGAAAACTACTCAATGAACTTTCAGAAAAATTAAAAGAAGAAAACATC
TACATTACATTGGGTGAAGATGTTCAAAAATACGTTATCAAAAACGGATTTAATGAACAATTTGGTGCAAGACCACTTAA
ACGTTATATCCAAAGAGACATAGAAACATTTATCGCAAAAGCAATCATTTCAGAAGATGTACTGCCAAATCATCACTATG
AAGTTATTGTAGACAATGATACATTAAACATTAAATTAGTCTCATAA

Upstream 100 bases:

>100_bases
GATTTGCCTATATCACTCATTATTAGCACTCATCACTTGACAGTGCCAATTATTCGGCATATAATAATGTTATATTATAG
AATTTTAAGGAGGAAATAAT

Downstream 100 bases:

>100_bases
ATTAAAATATCCCTATCACTAATTTGTGATAGGGATATCTTTATTTTTGGATTTGTTCTAACAAGTAACCTTCTCTAATA
CCTGATTTACTTACAATAAT

Product: ATP-dependent Clp protease ATP-binding subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 708; Mature: 708

Protein sequence:

>708_residues
MQFNPMDDYSKDPDILKKFGRNIVELVKEGKVDPVIGREEEIRRIIKILSRKTKNNPVLIGEPGVGKTAIVEGLARRIVD
KDVPLSLQNKIVYELDLAQLVAGAKFRGEFEERLKDVLNKIKESDGQIILFIDELHNIVGAGRADGAMDASNILKPMLAR
GELHCIGATTLNEYRLYIEKDSALERRFQKITVTEPTMEDTISILRGLKDRFEAHHGVHISDGAIIAATNLSARYITDRF
LPDKAIDLIDEACASIRMEIDSMPVELDDVQRKILQLEIEKSALSKETDTLSKERLSKIKSEIDDLKLEEASLKSRWEKE
KSQLNLSKRKKETLEQLKNELNTAYNEGNYSKAAELQYSKIPNLEKEISELSDTSTDDKLLTETVTPESIAEIVSKWTHI
PVTKLVSGDREKLRNLRLSLGKRVLGQDHALELISNAILRHRAGIKDPNRPIGSFLFLGPTGVGKTEAAKALAENLFDSE
NHIVRIDMSEYMESHSVSRLIGAPPGYVGYDQGGQLTEKIRRMPYAIVLFDEIEKAHPEVFNILLQVLDDGHLTDGQGRT
IDFKNTVIIMTSNIGSQYLLENASDAQEKIDKLLKQSFKPEFLNRIDEIITFNSLGFKVQVDITRKLLNELSEKLKEENI
YITLGEDVQKYVIKNGFNEQFGARPLKRYIQRDIETFIAKAIISEDVLPNHHYEVIVDNDTLNIKLVS

Sequences:

>Translated_708_residues
MQFNPMDDYSKDPDILKKFGRNIVELVKEGKVDPVIGREEEIRRIIKILSRKTKNNPVLIGEPGVGKTAIVEGLARRIVD
KDVPLSLQNKIVYELDLAQLVAGAKFRGEFEERLKDVLNKIKESDGQIILFIDELHNIVGAGRADGAMDASNILKPMLAR
GELHCIGATTLNEYRLYIEKDSALERRFQKITVTEPTMEDTISILRGLKDRFEAHHGVHISDGAIIAATNLSARYITDRF
LPDKAIDLIDEACASIRMEIDSMPVELDDVQRKILQLEIEKSALSKETDTLSKERLSKIKSEIDDLKLEEASLKSRWEKE
KSQLNLSKRKKETLEQLKNELNTAYNEGNYSKAAELQYSKIPNLEKEISELSDTSTDDKLLTETVTPESIAEIVSKWTHI
PVTKLVSGDREKLRNLRLSLGKRVLGQDHALELISNAILRHRAGIKDPNRPIGSFLFLGPTGVGKTEAAKALAENLFDSE
NHIVRIDMSEYMESHSVSRLIGAPPGYVGYDQGGQLTEKIRRMPYAIVLFDEIEKAHPEVFNILLQVLDDGHLTDGQGRT
IDFKNTVIIMTSNIGSQYLLENASDAQEKIDKLLKQSFKPEFLNRIDEIITFNSLGFKVQVDITRKLLNELSEKLKEENI
YITLGEDVQKYVIKNGFNEQFGARPLKRYIQRDIETFIAKAIISEDVLPNHHYEVIVDNDTLNIKLVS
>Mature_708_residues
MQFNPMDDYSKDPDILKKFGRNIVELVKEGKVDPVIGREEEIRRIIKILSRKTKNNPVLIGEPGVGKTAIVEGLARRIVD
KDVPLSLQNKIVYELDLAQLVAGAKFRGEFEERLKDVLNKIKESDGQIILFIDELHNIVGAGRADGAMDASNILKPMLAR
GELHCIGATTLNEYRLYIEKDSALERRFQKITVTEPTMEDTISILRGLKDRFEAHHGVHISDGAIIAATNLSARYITDRF
LPDKAIDLIDEACASIRMEIDSMPVELDDVQRKILQLEIEKSALSKETDTLSKERLSKIKSEIDDLKLEEASLKSRWEKE
KSQLNLSKRKKETLEQLKNELNTAYNEGNYSKAAELQYSKIPNLEKEISELSDTSTDDKLLTETVTPESIAEIVSKWTHI
PVTKLVSGDREKLRNLRLSLGKRVLGQDHALELISNAILRHRAGIKDPNRPIGSFLFLGPTGVGKTEAAKALAENLFDSE
NHIVRIDMSEYMESHSVSRLIGAPPGYVGYDQGGQLTEKIRRMPYAIVLFDEIEKAHPEVFNILLQVLDDGHLTDGQGRT
IDFKNTVIIMTSNIGSQYLLENASDAQEKIDKLLKQSFKPEFLNRIDEIITFNSLGFKVQVDITRKLLNELSEKLKEENI
YITLGEDVQKYVIKNGFNEQFGARPLKRYIQRDIETFIAKAIISEDVLPNHHYEVIVDNDTLNIKLVS

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:
GO:0000166: ATP-dependent Clp protease ATP-binding subunit
GO:0005524: ATP-dependent Clp protease ATP-binding subunit
GO:0008233: ATP-dependent Clp protease ATP-binding subunit
GO:0017111: ATP-dependent Clp protease ATP-binding subunit

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=306, Percent_Identity=37.9084967320261, Blast_Score=198, Evalue=1e-50,
Organism=Escherichia coli, GI1788943, Length=693, Percent_Identity=55.5555555555556, Blast_Score=788, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=287, Percent_Identity=47.7351916376307, Blast_Score=265, Evalue=1e-71,
Organism=Saccharomyces cerevisiae, GI6320464, Length=705, Percent_Identity=50.7801418439716, Blast_Score=695, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=707, Percent_Identity=42.8571428571429, Blast_Score=576, Evalue=1e-165,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 80108; Mature: 80108

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFNPMDDYSKDPDILKKFGRNIVELVKEGKVDPVIGREEEIRRIIKILSRKTKNNPVLI
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEE
GEPGVGKTAIVEGLARRIVDKDVPLSLQNKIVYELDLAQLVAGAKFRGEFEERLKDVLNK
ECCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECHHHHHHCCHHCCHHHHHHHHHHHH
IKESDGQIILFIDELHNIVGAGRADGAMDASNILKPMLARGELHCIGATTLNEYRLYIEK
HHCCCCCEEEEHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCEEEEEEC
DSALERRFQKITVTEPTMEDTISILRGLKDRFEAHHGVHISDGAIIAATNLSARYITDRF
CHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHCCEEECCCCEEEEECCCHHHHHHHC
LPDKAIDLIDEACASIRMEIDSMPVELDDVQRKILQLEIEKSALSKETDTLSKERLSKIK
CCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SEIDDLKLEEASLKSRWEKEKSQLNLSKRKKETLEQLKNELNTAYNEGNYSKAAELQYSK
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
IPNLEKEISELSDTSTDDKLLTETVTPESIAEIVSKWTHIPVTKLVSGDREKLRNLRLSL
CCCHHHHHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHH
GKRVLGQDHALELISNAILRHRAGIKDPNRPIGSFLFLGPTGVGKTEAAKALAENLFDSE
HHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCC
NHIVRIDMSEYMESHSVSRLIGAPPGYVGYDQGGQLTEKIRRMPYAIVLFDEIEKAHPEV
CCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCEEEEEEHHHHHHCHHH
FNILLQVLDDGHLTDGQGRTIDFKNTVIIMTSNIGSQYLLENASDAQEKIDKLLKQSFKP
HHHHHHHHCCCCCCCCCCCEEEECCEEEEEECCCCCHHHHCCCCHHHHHHHHHHHHCCCH
EFLNRIDEIITFNSLGFKVQVDITRKLLNELSEKLKEENIYITLGEDVQKYVIKNGFNEQ
HHHHHHHHHHEECCCCEEEEHHHHHHHHHHHHHHHHHCCEEEEECHHHHHHHHHCCCCCC
FGARPLKRYIQRDIETFIAKAIISEDVLPNHHYEVIVDNDTLNIKLVS
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCEEEEEEEC
>Mature Secondary Structure
MQFNPMDDYSKDPDILKKFGRNIVELVKEGKVDPVIGREEEIRRIIKILSRKTKNNPVLI
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEE
GEPGVGKTAIVEGLARRIVDKDVPLSLQNKIVYELDLAQLVAGAKFRGEFEERLKDVLNK
ECCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECHHHHHHCCHHCCHHHHHHHHHHHH
IKESDGQIILFIDELHNIVGAGRADGAMDASNILKPMLARGELHCIGATTLNEYRLYIEK
HHCCCCCEEEEHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCEEEEEEC
DSALERRFQKITVTEPTMEDTISILRGLKDRFEAHHGVHISDGAIIAATNLSARYITDRF
CHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHCCEEECCCCEEEEECCCHHHHHHHC
LPDKAIDLIDEACASIRMEIDSMPVELDDVQRKILQLEIEKSALSKETDTLSKERLSKIK
CCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SEIDDLKLEEASLKSRWEKEKSQLNLSKRKKETLEQLKNELNTAYNEGNYSKAAELQYSK
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
IPNLEKEISELSDTSTDDKLLTETVTPESIAEIVSKWTHIPVTKLVSGDREKLRNLRLSL
CCCHHHHHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHH
GKRVLGQDHALELISNAILRHRAGIKDPNRPIGSFLFLGPTGVGKTEAAKALAENLFDSE
HHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCC
NHIVRIDMSEYMESHSVSRLIGAPPGYVGYDQGGQLTEKIRRMPYAIVLFDEIEKAHPEV
CCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCEEEEEEHHHHHHCHHH
FNILLQVLDDGHLTDGQGRTIDFKNTVIIMTSNIGSQYLLENASDAQEKIDKLLKQSFKP
HHHHHHHHCCCCCCCCCCCEEEECCEEEEEECCCCCHHHHCCCCHHHHHHHHHHHHCCCH
EFLNRIDEIITFNSLGFKVQVDITRKLLNELSEKLKEENIYITLGEDVQKYVIKNGFNEQ
HHHHHHHHHHEECCCCEEEEHHHHHHHHHHHHHHHHHCCEEEEECHHHHHHHHHCCCCCC
FGARPLKRYIQRDIETFIAKAIISEDVLPNHHYEVIVDNDTLNIKLVS
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]