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The map label for this gene is flp [H]

Identifier: 162447964

GI number: 162447964

Start: 1147052

End: 1147747

Strand: Direct

Name: flp [H]

Synonym: ACL_1110

Alternate gene names: 162447964

Gene position: 1147052-1147747 (Clockwise)

Preceding gene: 162447956

Following gene: 162447975

Centisome position: 76.62

GC content: 35.49

Gene sequence:

>696_bases
ATGGAAGAGAAAAAATTACATCCACACAACTGCATTGATAGACTTGAACTATTCAACACACTTACAAGTGAACAACGTCA
ACTAGTGTTTAAATTGATTGAACACCGACATTATAGTGCCGGACAAATGATTTATAGTCCAGGTGAAAGTGCTGGTTCTA
TCCATATTATTAGTAGAGGAAAGGTTCGAATCTATCGACTCTCTGAAAGCGGTCGTGAACAACTTATAAGACTTTTACTC
CCAGGTGAATTTACTGGAGAGCTTGCACTATTTAAAGAAGGCATCTATGAAGCTTATGCTGAAAGTCTAGAAGATACAAA
GATATGTATGATCCACCATGACGACTTTAAAAACTTACTTAAATCCTACCCTGCCATATCAGTTAAGATGTTATCTGTTT
TAGCAAAGCGCTTAAGTATCTCTGAACAACAATCAACCTGGATGTCTACAGAAACATCCAAAGAAAGACTAATTAACTTT
CTTATCCGAAGTGCAACACTCAATAAGCAAGGTGAGATGGTAGTCTATCTACCTATGCCTAAAAAAGATCTTGCATCTTA
TTTAGGCACAACTCCCGAAACCTTATCGCGCCAATTTACAATATTAAAAAAAGACGGTGTCATTACACAGTCATCTAAAA
ATTATGTAAAGATTCATGGTTTAAGCCATAATGATATTACATGTGAGATTTGTTAA

Upstream 100 bases:

>100_bases
CATAGTTTATGACTATCACCATTATAAAGAAATCAAAGTTATAAATAATTGATAGTTGTCAATTTATTGATATAATGTAA
TAAAGAAAGTTGGTAAATAT

Downstream 100 bases:

>100_bases
GCACATATAAAAAACGCACTTTATGAGTGCGTTTTATTTATAAGTTACCTATTCATCATATATTTTTCTAATCTGTTTTT
TGAATCTTCACTTAATGCTT

Product: Crp/Fnr family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MEEKKLHPHNCIDRLELFNTLTSEQRQLVFKLIEHRHYSAGQMIYSPGESAGSIHIISRGKVRIYRLSESGREQLIRLLL
PGEFTGELALFKEGIYEAYAESLEDTKICMIHHDDFKNLLKSYPAISVKMLSVLAKRLSISEQQSTWMSTETSKERLINF
LIRSATLNKQGEMVVYLPMPKKDLASYLGTTPETLSRQFTILKKDGVITQSSKNYVKIHGLSHNDITCEIC

Sequences:

>Translated_231_residues
MEEKKLHPHNCIDRLELFNTLTSEQRQLVFKLIEHRHYSAGQMIYSPGESAGSIHIISRGKVRIYRLSESGREQLIRLLL
PGEFTGELALFKEGIYEAYAESLEDTKICMIHHDDFKNLLKSYPAISVKMLSVLAKRLSISEQQSTWMSTETSKERLINF
LIRSATLNKQGEMVVYLPMPKKDLASYLGTTPETLSRQFTILKKDGVITQSSKNYVKIHGLSHNDITCEIC
>Mature_231_residues
MEEKKLHPHNCIDRLELFNTLTSEQRQLVFKLIEHRHYSAGQMIYSPGESAGSIHIISRGKVRIYRLSESGREQLIRLLL
PGEFTGELALFKEGIYEAYAESLEDTKICMIHHDDFKNLLKSYPAISVKMLSVLAKRLSISEQQSTWMSTETSKERLINF
LIRSATLNKQGEMVVYLPMPKKDLASYLGTTPETLSRQFTILKKDGVITQSSKNYVKIHGLSHNDITCEIC

Specific function: Global Transcription Factor That Controls The Expression Of Over 100 Target Genes In Response To Anoxia. It Facilitates The Adaptation To Anaerobic Growth Conditions By Regulating The Expression Of Gene Products That Are Involved In Anaerobic Energy Metab

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787595, Length=230, Percent_Identity=24.7826086956522, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI1789756, Length=188, Percent_Identity=23.4042553191489, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 26437; Mature: 26437

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEKKLHPHNCIDRLELFNTLTSEQRQLVFKLIEHRHYSAGQMIYSPGESAGSIHIISRG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCEEEEEECC
KVRIYRLSESGREQLIRLLLPGEFTGELALFKEGIYEAYAESLEDTKICMIHHDDFKNLL
CEEEEEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHH
KSYPAISVKMLSVLAKRLSISEQQSTWMSTETSKERLINFLIRSATLNKQGEMVVYLPMP
HHCCCHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCC
KKDLASYLGTTPETLSRQFTILKKDGVITQSSKNYVKIHGLSHNDITCEIC
HHHHHHHHCCCHHHHHHEEEEEECCCEEEECCCCEEEEEECCCCCEEEEEC
>Mature Secondary Structure
MEEKKLHPHNCIDRLELFNTLTSEQRQLVFKLIEHRHYSAGQMIYSPGESAGSIHIISRG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCEEEEEECC
KVRIYRLSESGREQLIRLLLPGEFTGELALFKEGIYEAYAESLEDTKICMIHHDDFKNLL
CEEEEEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHH
KSYPAISVKMLSVLAKRLSISEQQSTWMSTETSKERLINFLIRSATLNKQGEMVVYLPMP
HHCCCHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCC
KKDLASYLGTTPETLSRQFTILKKDGVITQSSKNYVKIHGLSHNDITCEIC
HHHHHHHHCCCHHHHHHEEEEEECCCEEEECCCCEEEEEECCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2113923; 8441692 [H]