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The map label for this gene is aes [H]

Identifier: 162447505

GI number: 162447505

Start: 672858

End: 673826

Strand: Direct

Name: aes [H]

Synonym: ACL_0645

Alternate gene names: 162447505

Gene position: 672858-673826 (Clockwise)

Preceding gene: 162447504

Following gene: 162447506

Centisome position: 44.95

GC content: 33.95

Gene sequence:

>969_bases
ATGAAGTCTAAAGATCAGGCAATTGATTCTGAATTGAGACTCACAGGTAAATTTATTCGTTTTTTTACGCCTTCGTTTAA
AGTAGGAACATTAAAACTTGCAAAAAAACTTATGGGTGCTTTAAAAGGTAAAGCACCAGCAAAACTCTTTTATGAAGAAG
TACATATACCGAGACTAGATGGAACACTTTTAAGACTGACTATATATGCACCAATAAAAAGAAAATATAATGCACCAGGA
CTTCTTTATATGCATGGTGGTGGTTACGGTTTAGGTATTCCTGAGATGAATGAGAAGTTATTTAAACTGCTCATTGATGA
AACAGGTTGTGTCATTATTTCACCAGATTACACTTTATCTGTTGAAAAACCATATCCAGCAGCTATAGATGATTGTTATC
TAGCGCTTTTATGGATGAAGAAAAATCATTTACGTTACATGATTAATCCGAATCAACTCATGATTGCAGGTGATTCTGCA
GGTGGTGGCTTAACGGCAGCATTGGCACTACTTGCTAGAGACAAAAATGAAGTTCAAATTGCATTTCAAATGCCACTTTA
TCCAATGATTGATGATCGTATGAACACACCATCATCAATTGATAATGATGATCCAGTGTGGAACTCTAAGCAAAACAAAC
TTGCTTGGGAACTTTATTTGGGTGAATTATTTGGTAAAGATGAAGTACCTTATTATGCGGCACCAGCAAGAGCTCAAAAT
TTAAAGAATTTACCACCAACGATGACCTATGTTGGAAGTGTAGAACCTTTTTTAGATGAAACAAGAGATTTTGTAACAAG
ACTCAAACTACAAGGCAACGACGTTAATTTCAAGATTTTTGAAGGTGGATATCACGCATTTGATATCCTAAGTGATAAAA
CTCAAATTGGCATTGCTGCTAAGAATTATTTACTAGACAATTTTAAATATGCAATAAAAAATCACTTTAAGGAAAATAAG
AACATATGA

Upstream 100 bases:

>100_bases
ATATGTTAGAAGCTTTAAAATCTTTCGAATAATCAAATTGGTCAATTACTTGGTATAATAGGCGTAGTATGTAAATACTA
AGCATTAGGGGAGAAACTAT

Downstream 100 bases:

>100_bases
GCACATTAACATTTAACATCGAAGATAAATATGTAGGTATTACAATTAGAGAATACCTTCAAACTTATAACGTAGGAAAA
CCCGTAATCTATAAGTATTC

Product: esterase/lipase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MKSKDQAIDSELRLTGKFIRFFTPSFKVGTLKLAKKLMGALKGKAPAKLFYEEVHIPRLDGTLLRLTIYAPIKRKYNAPG
LLYMHGGGYGLGIPEMNEKLFKLLIDETGCVIISPDYTLSVEKPYPAAIDDCYLALLWMKKNHLRYMINPNQLMIAGDSA
GGGLTAALALLARDKNEVQIAFQMPLYPMIDDRMNTPSSIDNDDPVWNSKQNKLAWELYLGELFGKDEVPYYAAPARAQN
LKNLPPTMTYVGSVEPFLDETRDFVTRLKLQGNDVNFKIFEGGYHAFDILSDKTQIGIAAKNYLLDNFKYAIKNHFKENK
NI

Sequences:

>Translated_322_residues
MKSKDQAIDSELRLTGKFIRFFTPSFKVGTLKLAKKLMGALKGKAPAKLFYEEVHIPRLDGTLLRLTIYAPIKRKYNAPG
LLYMHGGGYGLGIPEMNEKLFKLLIDETGCVIISPDYTLSVEKPYPAAIDDCYLALLWMKKNHLRYMINPNQLMIAGDSA
GGGLTAALALLARDKNEVQIAFQMPLYPMIDDRMNTPSSIDNDDPVWNSKQNKLAWELYLGELFGKDEVPYYAAPARAQN
LKNLPPTMTYVGSVEPFLDETRDFVTRLKLQGNDVNFKIFEGGYHAFDILSDKTQIGIAAKNYLLDNFKYAIKNHFKENK
NI
>Mature_322_residues
MKSKDQAIDSELRLTGKFIRFFTPSFKVGTLKLAKKLMGALKGKAPAKLFYEEVHIPRLDGTLLRLTIYAPIKRKYNAPG
LLYMHGGGYGLGIPEMNEKLFKLLIDETGCVIISPDYTLSVEKPYPAAIDDCYLALLWMKKNHLRYMINPNQLMIAGDSA
GGGLTAALALLARDKNEVQIAFQMPLYPMIDDRMNTPSSIDNDDPVWNSKQNKLAWELYLGELFGKDEVPYYAAPARAQN
LKNLPPTMTYVGSVEPFLDETRDFVTRLKLQGNDVNFKIFEGGYHAFDILSDKTQIGIAAKNYLLDNFKYAIKNHFKENK
NI

Specific function: Displays esterase activity towards short chain fatty esters (acyl chain length of up to 8 carbons). Able to hydrolyze triacetylglycerol (triacetin) and tributyrylglycerol (tributyrin), but not trioleylglycerol (triolein) or cholesterol oleate. Negatively

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:
GO:0008152: Esterase/lipase
GO:0016787: Esterase/lipase

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68299767, Length=323, Percent_Identity=27.8637770897833, Blast_Score=76, Evalue=4e-14,
Organism=Escherichia coli, GI1786682, Length=212, Percent_Identity=30.188679245283, Blast_Score=87, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI72001146, Length=177, Percent_Identity=29.3785310734463, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17567059, Length=120, Percent_Identity=34.1666666666667, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17540028, Length=126, Percent_Identity=32.5396825396825, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71996133, Length=127, Percent_Identity=32.2834645669291, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.-

Molecular weight: Translated: 36376; Mature: 36376

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSKDQAIDSELRLTGKFIRFFTPSFKVGTLKLAKKLMGALKGKAPAKLFYEEVHIPRLD
CCCCCHHHCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCC
GTLLRLTIYAPIKRKYNAPGLLYMHGGGYGLGIPEMNEKLFKLLIDETGCVIISPDYTLS
CEEEEEEEEECHHHCCCCCEEEEECCCCEECCCCHHHHHHHHHHHCCCCEEEECCCCEEE
VEKPYPAAIDDCYLALLWMKKNHLRYMINPNQLMIAGDSAGGGLTAALALLARDKNEVQI
ECCCCCCHHHHHHHHHHEEECCCEEEEECCCEEEEEECCCCCHHHHHHHHHHCCCCCEEE
AFQMPLYPMIDDRMNTPSSIDNDDPVWNSKQNKLAWELYLGELFGKDEVPYYAAPARAQN
EEECCCCCHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEEHHHCCCCCCCEEECCHHHHH
LKNLPPTMTYVGSVEPFLDETRDFVTRLKLQGNDVNFKIFEGGYHAFDILSDKTQIGIAA
CCCCCCCEEEECCCCHHHHHHHHHHHEEEECCCCEEEEEEECCCEEEEEECCCCEEEEEH
KNYLLDNFKYAIKNHFKENKNI
HHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKSKDQAIDSELRLTGKFIRFFTPSFKVGTLKLAKKLMGALKGKAPAKLFYEEVHIPRLD
CCCCCHHHCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCC
GTLLRLTIYAPIKRKYNAPGLLYMHGGGYGLGIPEMNEKLFKLLIDETGCVIISPDYTLS
CEEEEEEEEECHHHCCCCCEEEEECCCCEECCCCHHHHHHHHHHHCCCCEEEECCCCEEE
VEKPYPAAIDDCYLALLWMKKNHLRYMINPNQLMIAGDSAGGGLTAALALLARDKNEVQI
ECCCCCCHHHHHHHHHHEEECCCEEEEECCCEEEEEECCCCCHHHHHHHHHHCCCCCEEE
AFQMPLYPMIDDRMNTPSSIDNDDPVWNSKQNKLAWELYLGELFGKDEVPYYAAPARAQN
EEECCCCCHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEEHHHCCCCCCCEEECCHHHHH
LKNLPPTMTYVGSVEPFLDETRDFVTRLKLQGNDVNFKIFEGGYHAFDILSDKTQIGIAA
CCCCCCCEEEECCCCHHHHHHHHHHHEEEECCCCEEEEEEECCCEEEEEECCCCEEEEEH
KNYLLDNFKYAIKNHFKENKNI
HHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA