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The map label for this gene is hinfIM [H]

Identifier: 162447456

GI number: 162447456

Start: 629457

End: 630335

Strand: Direct

Name: hinfIM [H]

Synonym: ACL_0595

Alternate gene names: 162447456

Gene position: 629457-630335 (Clockwise)

Preceding gene: 162447455

Following gene: 162447457

Centisome position: 42.05

GC content: 37.32

Gene sequence:

>879_bases
ATGTCACTCTTTGGATTTGAGGAACTAGAAGCAAATGTACCAGATAGTGCTACCGATGATGACTTTGATCCAACAGATGA
TCTATCAGATGTTCCTTATTCTCAAAAAGGCGATATTTATATCTTGGGTAATCACCGAGTGATGTGTGGTGATTCTACAG
AAAAAACTGATGTTAAGAAACTGATCCAAGATGATCGGATTGATCTAGTTTTTACAGATCCACCATACAATGTTGATTAT
GAAGGCACAGCTGGAAAAATAATGAACGATAAGATGGAAGATAATACCTTCTATCTTTTTCTTTATAAAGCCTTTGAGAA
TATGTTTGAGCATACGAAACCAGGTGGTGCTATTTATGTATGTCATGCAGACACTGAAGGACTCAACTTTAGAAATGCCT
TCAAGAATGCTGGGTATAAACTCGCAGAGTGTCTCATTTGGGTAAAGAATGCACTAGTGTTAGGTAGACAAGATTATCAC
TGGCGACATGAACCTATTCTTTATGGTTGGAGAGAAGGTGCTGCTCATTACTTTGTGGATGACCGCACACAAGATACCAT
CTGGGAATATAACAAACCAAAACGAAATGAAGAACATCCAACAATGAAACCTTTAGAACTCGTCGGTAAAGCTATCGCTA
ATTCATCCAGAGTAAATGAGATAGTCTTAGATTTATTTGGTGGTTCTGGATCAACCATGATCGCATCTGATCAGCTTCAA
AGAAGAGCAAGAATCATGGAACTGGATGAACGATTTGTGGATGTGATTGTAAAACGCTATTTAAAGTACAAAGTATCACT
TGATGATTGTTATCTCATTCGAAATGGAAAAGAGATACCGCTTAGTCATATCAACGACTTTCAGATATTGTCACTATAG

Upstream 100 bases:

>100_bases
ACAAATCAAAGCATTCAGACTTGCGGATAATAAAACAGCAGAACTTGCGACTTGGGATTTTGCAAAACTCGAAGAAGAAC
TAGCAAACATTGAAATGGAT

Downstream 100 bases:

>100_bases
TGAGCAAATCTTGTCGAATTGACTTGCTATTTAATGCCTTTAGAGTGATATATAGTAGTAACCAAAAAAGGTTAGGAAAG
AGGCAATAGCGATGAAAGTC

Product: DNA modification methylase

Products: NA

Alternate protein names: M.HinfI; Adenine-specific methyltransferase HinfI [H]

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MSLFGFEELEANVPDSATDDDFDPTDDLSDVPYSQKGDIYILGNHRVMCGDSTEKTDVKKLIQDDRIDLVFTDPPYNVDY
EGTAGKIMNDKMEDNTFYLFLYKAFENMFEHTKPGGAIYVCHADTEGLNFRNAFKNAGYKLAECLIWVKNALVLGRQDYH
WRHEPILYGWREGAAHYFVDDRTQDTIWEYNKPKRNEEHPTMKPLELVGKAIANSSRVNEIVLDLFGGSGSTMIASDQLQ
RRARIMELDERFVDVIVKRYLKYKVSLDDCYLIRNGKEIPLSHINDFQILSL

Sequences:

>Translated_292_residues
MSLFGFEELEANVPDSATDDDFDPTDDLSDVPYSQKGDIYILGNHRVMCGDSTEKTDVKKLIQDDRIDLVFTDPPYNVDY
EGTAGKIMNDKMEDNTFYLFLYKAFENMFEHTKPGGAIYVCHADTEGLNFRNAFKNAGYKLAECLIWVKNALVLGRQDYH
WRHEPILYGWREGAAHYFVDDRTQDTIWEYNKPKRNEEHPTMKPLELVGKAIANSSRVNEIVLDLFGGSGSTMIASDQLQ
RRARIMELDERFVDVIVKRYLKYKVSLDDCYLIRNGKEIPLSHINDFQILSL
>Mature_291_residues
SLFGFEELEANVPDSATDDDFDPTDDLSDVPYSQKGDIYILGNHRVMCGDSTEKTDVKKLIQDDRIDLVFTDPPYNVDYE
GTAGKIMNDKMEDNTFYLFLYKAFENMFEHTKPGGAIYVCHADTEGLNFRNAFKNAGYKLAECLIWVKNALVLGRQDYHW
RHEPILYGWREGAAHYFVDDRTQDTIWEYNKPKRNEEHPTMKPLELVGKAIANSSRVNEIVLDLFGGSGSTMIASDQLQR
RARIMELDERFVDVIVKRYLKYKVSLDDCYLIRNGKEIPLSHINDFQILSL

Specific function: This methylase recognizes the double-stranded sequence GANTC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the HinfI endonuclease [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR002052
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 33709; Mature: 33578

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLFGFEELEANVPDSATDDDFDPTDDLSDVPYSQKGDIYILGNHRVMCGDSTEKTDVKK
CCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCEEEECCCCCHHHHHH
LIQDDRIDLVFTDPPYNVDYEGTAGKIMNDKMEDNTFYLFLYKAFENMFEHTKPGGAIYV
HHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHCCCCCEEEE
CHADTEGLNFRNAFKNAGYKLAECLIWVKNALVLGRQDYHWRHEPILYGWREGAAHYFVD
EECCCCCCCHHHHHHHCCHHHHHHHHHHHHHHEECCCCCCCCCCCEEEEECCCCEEEEEC
DRTQDTIWEYNKPKRNEEHPTMKPLELVGKAIANSSRVNEIVLDLFGGSGSTMIASDQLQ
CCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHEEEEEEECCCCCEEEEHHHHH
RRARIMELDERFVDVIVKRYLKYKVSLDDCYLIRNGKEIPLSHINDFQILSL
HHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEEECCCCCCCCCCCCEEEEEC
>Mature Secondary Structure 
SLFGFEELEANVPDSATDDDFDPTDDLSDVPYSQKGDIYILGNHRVMCGDSTEKTDVKK
CCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCEEEECCCCCHHHHHH
LIQDDRIDLVFTDPPYNVDYEGTAGKIMNDKMEDNTFYLFLYKAFENMFEHTKPGGAIYV
HHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHCCCCCEEEE
CHADTEGLNFRNAFKNAGYKLAECLIWVKNALVLGRQDYHWRHEPILYGWREGAAHYFVD
EECCCCCCCHHHHHHHCCHHHHHHHHHHHHHHEECCCCCCCCCCCEEEEECCCCEEEEEC
DRTQDTIWEYNKPKRNEEHPTMKPLELVGKAIANSSRVNEIVLDLFGGSGSTMIASDQLQ
CCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHEEEEEEECCCCCEEEEHHHHH
RRARIMELDERFVDVIVKRYLKYKVSLDDCYLIRNGKEIPLSHINDFQILSL
HHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEEECCCCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3063606 [H]