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The map label for this gene is nadE [H]

Identifier: 162447420

GI number: 162447420

Start: 593369

End: 595219

Strand: Direct

Name: nadE [H]

Synonym: ACL_0558

Alternate gene names: 162447420

Gene position: 593369-595219 (Clockwise)

Preceding gene: 162447419

Following gene: 162447421

Centisome position: 39.64

GC content: 33.71

Gene sequence:

>1851_bases
ATGACACAACACGGTATATTAAAAGTTTCACTTGCAAGTCCTGAAATCAAATTAGGTAATCCACTGCTCAATGTGGAACC
TATTTTAAATATACTTAATCATGAATCCTCTGGGTTAGTTTTATTTCCTGAACTATCATTAACTGGTTACACCTCAGGAG
ATTTATTTTTCCAATTAGATTACATCAATAAAACAAATGAAGCACTAAGTAAAATTATGAATGGAACAACTTATCAAGGT
GTGTACATATTAGGTATGCCTTTAAAAGTAGGGGATGCACTTTTTAATGTTGCAGTAGTTATTCAAAATAAGAAGATACT
TGGTGTGATACCTAAGTATCACCTACCAAATTCAAAAGAGTTTAATGAAAAGCGCTGGTTCCAATCAGGTTTTAACTATG
AGCTTGATACAGTGGAAGTACTTGGACAAACCGTCCCATTTGGTGATATGGTCTTTAAAGATGAATTACATGACATTGGA
TTTGGTGTAGAAATATGCCAAGATATGTGGGCGATTGAGACACCTGGAAATCATTTAAGTCTAAGTGGTGCTCATCTGAT
TTTAAACTTATCGGCCTCACCTGAAACCATGGGTAAACAAACGGTTAGAAAACACACTGTATTAGATGCTTCAAGAAGAC
AGTTAAGTGGTTATTTATACACTGCTTCTGCATCATCAGAATCAACAATGGATGTCGTGTTTACACCACATAAGATAGCA
GCTAGTTTGGGTAGTTTGATTATTGAATCTAGTCTATTAGATCCTAAAGAAGTTTTAAGAGTAGACATGGACATAGAAGC
TATTCGCTATCAAAGAAGAATTGATTCTACCTTTAAAGATGAACAAAATGGCTATACACAATCAACAACTAAAGTTCATG
TAGACTTTTTTACTAACAATGACTATGTATTTGAAAGACCTTTAAACAAAACACCATTCCTACCTAAAGTAGGCGATAAA
GAAAGTTTACTGATTGCTAATCAAATACAAGTACAAGGTTTAGTTCAAAAAATCAAACATCTAGGAAAACCTAAGATTAT
TATTGGTATCTCAGGTGGTTTGGATAGTACACTTGCTTTATTAGTTGCTCATCAAGCAATTAAAAAACTAAATGGTGATG
TTAAAGATATCATCGGTGTTACTATGCCTAGTCAAGTAACCTCTAAAGGTTCTAAGACAGATGCCATCTTACTCATGGAA
AAATTAGGTATCACAGCACTTGAAATTCCAATTGAAGATCAAGTAAAACTTCATCTTGAAAGTTTAAATCATGGTGGTAG
TACAGATATTACCTATGAAAATGCACAGGCTAGAATCCGTACACTCATATTAATGAATCTATCAAACAAACACCAAGGAT
TTGTTTTAGGTACAGGTGACTTAAGCGAAATAGCGCTTGGTTGGATGACTTTTAATGGTGATCATATGAGTATGTATAAT
GTCAATGCTGGTGTACCTAAAACACTTGTTCAAGCACTTGTTAAGCTGCACAGTGAACATAACTACCAAGATATTAAAGA
TATATTAATAAGTATTTTAAACCGACCAATCAGCCCAGAATTAAAAGAAAATCAAAAAACTGAGGATGTCATTGGTACAT
ATGAAATTAATGATTTTATGCTGCATCATTTATTAGAAAATGGTGCGAGTGAAGAAAAATTATCCTTCCTAGTTAGACAC
ACATTTGGTCTATCTGTAGAAGCATCAGATCTTTATGCAAAAAGATTATTAAACAGGTTTTATCAGTCACAGTTTAAGCG
TCAAGTGATGCCTGAGGGACCAAAAGTGTTAAGGATAAGCTTATCACCTAGAGGTGATTTACGCTTACCTTCAGATATGA
ATAGAAAGTAG

Upstream 100 bases:

>100_bases
GTTCTGAAATTAGAAAAAACTTAAATCAAAATTTAGACAAATTAAACTTAAATGTAAAAACATATATTTTAGAAAATAAA
CTCTATGAAGAGGTGAAAGT

Downstream 100 bases:

>100_bases
TGTTATTTTGAAAAAGAAAGTATTAATCGGACTATCAGGCGGAGTCGACTCTTCAGTCGCTGCTTTGATACTGTTACAAC
AAGGCTATGATGTAGAAGCG

Product: NAD(+) synthase, glutamine-dependent

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 616; Mature: 615

Protein sequence:

>616_residues
MTQHGILKVSLASPEIKLGNPLLNVEPILNILNHESSGLVLFPELSLTGYTSGDLFFQLDYINKTNEALSKIMNGTTYQG
VYILGMPLKVGDALFNVAVVIQNKKILGVIPKYHLPNSKEFNEKRWFQSGFNYELDTVEVLGQTVPFGDMVFKDELHDIG
FGVEICQDMWAIETPGNHLSLSGAHLILNLSASPETMGKQTVRKHTVLDASRRQLSGYLYTASASSESTMDVVFTPHKIA
ASLGSLIIESSLLDPKEVLRVDMDIEAIRYQRRIDSTFKDEQNGYTQSTTKVHVDFFTNNDYVFERPLNKTPFLPKVGDK
ESLLIANQIQVQGLVQKIKHLGKPKIIIGISGGLDSTLALLVAHQAIKKLNGDVKDIIGVTMPSQVTSKGSKTDAILLME
KLGITALEIPIEDQVKLHLESLNHGGSTDITYENAQARIRTLILMNLSNKHQGFVLGTGDLSEIALGWMTFNGDHMSMYN
VNAGVPKTLVQALVKLHSEHNYQDIKDILISILNRPISPELKENQKTEDVIGTYEINDFMLHHLLENGASEEKLSFLVRH
TFGLSVEASDLYAKRLLNRFYQSQFKRQVMPEGPKVLRISLSPRGDLRLPSDMNRK

Sequences:

>Translated_616_residues
MTQHGILKVSLASPEIKLGNPLLNVEPILNILNHESSGLVLFPELSLTGYTSGDLFFQLDYINKTNEALSKIMNGTTYQG
VYILGMPLKVGDALFNVAVVIQNKKILGVIPKYHLPNSKEFNEKRWFQSGFNYELDTVEVLGQTVPFGDMVFKDELHDIG
FGVEICQDMWAIETPGNHLSLSGAHLILNLSASPETMGKQTVRKHTVLDASRRQLSGYLYTASASSESTMDVVFTPHKIA
ASLGSLIIESSLLDPKEVLRVDMDIEAIRYQRRIDSTFKDEQNGYTQSTTKVHVDFFTNNDYVFERPLNKTPFLPKVGDK
ESLLIANQIQVQGLVQKIKHLGKPKIIIGISGGLDSTLALLVAHQAIKKLNGDVKDIIGVTMPSQVTSKGSKTDAILLME
KLGITALEIPIEDQVKLHLESLNHGGSTDITYENAQARIRTLILMNLSNKHQGFVLGTGDLSEIALGWMTFNGDHMSMYN
VNAGVPKTLVQALVKLHSEHNYQDIKDILISILNRPISPELKENQKTEDVIGTYEINDFMLHHLLENGASEEKLSFLVRH
TFGLSVEASDLYAKRLLNRFYQSQFKRQVMPEGPKVLRISLSPRGDLRLPSDMNRK
>Mature_615_residues
TQHGILKVSLASPEIKLGNPLLNVEPILNILNHESSGLVLFPELSLTGYTSGDLFFQLDYINKTNEALSKIMNGTTYQGV
YILGMPLKVGDALFNVAVVIQNKKILGVIPKYHLPNSKEFNEKRWFQSGFNYELDTVEVLGQTVPFGDMVFKDELHDIGF
GVEICQDMWAIETPGNHLSLSGAHLILNLSASPETMGKQTVRKHTVLDASRRQLSGYLYTASASSESTMDVVFTPHKIAA
SLGSLIIESSLLDPKEVLRVDMDIEAIRYQRRIDSTFKDEQNGYTQSTTKVHVDFFTNNDYVFERPLNKTPFLPKVGDKE
SLLIANQIQVQGLVQKIKHLGKPKIIIGISGGLDSTLALLVAHQAIKKLNGDVKDIIGVTMPSQVTSKGSKTDAILLMEK
LGITALEIPIEDQVKLHLESLNHGGSTDITYENAQARIRTLILMNLSNKHQGFVLGTGDLSEIALGWMTFNGDHMSMYNV
NAGVPKTLVQALVKLHSEHNYQDIKDILISILNRPISPELKENQKTEDVIGTYEINDFMLHHLLENGASEEKLSFLVRHT
FGLSVEASDLYAKRLLNRFYQSQFKRQVMPEGPKVLRISLSPRGDLRLPSDMNRK

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:
GO:0000166: NAD(+) synthase, glutamine-dependent
GO:0003952: NAD(+) synthase, glutamine-dependent
GO:0005524: NAD(+) synthase, glutamine-dependent
GO:0006807: NAD(+) synthase, glutamine-dependent
GO:0008795: NAD(+) synthase, glutamine-dependent
GO:0009435: NAD(+) synthase, glutamine-dependent
GO:0016810: NAD(+) synthase, glutamine-dependent
GO:0016874: NAD(+) synthase, glutamine-dependent

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI41393551, Length=559, Percent_Identity=23.7924865831843, Blast_Score=112, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17538756, Length=191, Percent_Identity=28.2722513089005, Blast_Score=87, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6321865, Length=587, Percent_Identity=21.4650766609881, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24641841, Length=584, Percent_Identity=23.6301369863014, Blast_Score=113, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24641843, Length=584, Percent_Identity=23.6301369863014, Blast_Score=113, Evalue=4e-25,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 68690; Mature: 68559

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQHGILKVSLASPEIKLGNPLLNVEPILNILNHESSGLVLFPELSLTGYTSGDLFFQLD
CCCCCEEEEEECCCCEECCCCCCCHHHHHHHHCCCCCCEEEEECCEECCCCCCCEEEEEE
YINKTNEALSKIMNGTTYQGVYILGMPLKVGDALFNVAVVIQNKKILGVIPKYHLPNSKE
CCCCHHHHHHHHHCCCCCCCEEEEECCHHHHHHHEEEEEEEECCEEEEECCCCCCCCCCC
FNEKRWFQSGFNYELDTVEVLGQTVPFGDMVFKDELHDIGFGVEICQDMWAIETPGNHLS
CCHHHHHHCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHEECCCCCEEE
LSGAHLILNLSASPETMGKQTVRKHTVLDASRRQLSGYLYTASASSESTMDVVFTPHKIA
ECCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCEEEEECCHHHH
ASLGSLIIESSLLDPKEVLRVDMDIEAIRYQRRIDSTFKDEQNGYTQSTTKVHVDFFTNN
HHHHHHHHHHCCCCHHHHHEECCCHHHHHHHHHHCCHHHHCCCCCCCCCEEEEEEEEECC
DYVFERPLNKTPFLPKVGDKESLLIANQIQVQGLVQKIKHLGKPKIIIGISGGLDSTLAL
CEEEECCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHH
LVAHQAIKKLNGDVKDIIGVTMPSQVTSKGSKTDAILLMEKLGITALEIPIEDQVKLHLE
HHHHHHHHHHCCCHHHHHCCCCCHHHHCCCCCCCCEEEEHHCCCEEEECCCCCCHHEEEE
SLNHGGSTDITYENAQARIRTLILMNLSNKHQGFVLGTGDLSEIALGWMTFNGDHMSMYN
CCCCCCCCCEEECCHHHEEEEEEEEECCCCCCEEEEECCCHHHHEEEEEEECCCEEEEEE
VNAGVPKTLVQALVKLHSEHNYQDIKDILISILNRPISPELKENQKTEDVIGTYEINDFM
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHCCCCHHHEEEEEEHHHHH
LHHLLENGASEEKLSFLVRHTFGLSVEASDLYAKRLLNRFYQSQFKRQVMPEGPKVLRIS
HHHHHHCCCCHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
LSPRGDLRLPSDMNRK
ECCCCCCCCCCCCCCC
>Mature Secondary Structure 
TQHGILKVSLASPEIKLGNPLLNVEPILNILNHESSGLVLFPELSLTGYTSGDLFFQLD
CCCCEEEEEECCCCEECCCCCCCHHHHHHHHCCCCCCEEEEECCEECCCCCCCEEEEEE
YINKTNEALSKIMNGTTYQGVYILGMPLKVGDALFNVAVVIQNKKILGVIPKYHLPNSKE
CCCCHHHHHHHHHCCCCCCCEEEEECCHHHHHHHEEEEEEEECCEEEEECCCCCCCCCCC
FNEKRWFQSGFNYELDTVEVLGQTVPFGDMVFKDELHDIGFGVEICQDMWAIETPGNHLS
CCHHHHHHCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHEECCCCCEEE
LSGAHLILNLSASPETMGKQTVRKHTVLDASRRQLSGYLYTASASSESTMDVVFTPHKIA
ECCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCEEEEECCHHHH
ASLGSLIIESSLLDPKEVLRVDMDIEAIRYQRRIDSTFKDEQNGYTQSTTKVHVDFFTNN
HHHHHHHHHHCCCCHHHHHEECCCHHHHHHHHHHCCHHHHCCCCCCCCCEEEEEEEEECC
DYVFERPLNKTPFLPKVGDKESLLIANQIQVQGLVQKIKHLGKPKIIIGISGGLDSTLAL
CEEEECCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHH
LVAHQAIKKLNGDVKDIIGVTMPSQVTSKGSKTDAILLMEKLGITALEIPIEDQVKLHLE
HHHHHHHHHHCCCHHHHHCCCCCHHHHCCCCCCCCEEEEHHCCCEEEECCCCCCHHEEEE
SLNHGGSTDITYENAQARIRTLILMNLSNKHQGFVLGTGDLSEIALGWMTFNGDHMSMYN
CCCCCCCCCEEECCHHHEEEEEEEEECCCCCCEEEEECCCHHHHEEEEEEECCCEEEEEE
VNAGVPKTLVQALVKLHSEHNYQDIKDILISILNRPISPELKENQKTEDVIGTYEINDFM
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHCCCCHHHEEEEEEHHHHH
LHHLLENGASEEKLSFLVRHTFGLSVEASDLYAKRLLNRFYQSQFKRQVMPEGPKVLRIS
HHHHHHCCCCHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
LSPRGDLRLPSDMNRK
ECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]