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The map label for this gene is grpE

Identifier: 162447411

GI number: 162447411

Start: 585094

End: 585666

Strand: Direct

Name: grpE

Synonym: ACL_0549

Alternate gene names: 162447411

Gene position: 585094-585666 (Clockwise)

Preceding gene: 162447410

Following gene: 162447412

Centisome position: 39.08

GC content: 29.84

Gene sequence:

>573_bases
ATGGCAGATAAAGAAAAAGATGCAGTTATCGTTGATGAAACGGAACATGTAGATGTAGATTCAAAAGAATCGAAAAAAGA
AAAGAAAACAAAACAACAACAAAAAATTGAATTCTTAGAGGCTGAAGTAAAAGAACTAAATGATAAATACTTAAGAACCT
TGGCAGAAGCTGAAAACTTTAAAAAACGTATTCAAGCAGAAAAGATAATGGATCGTAAGTATGCAGCAAGCAGTTTTGCT
ACAGAATTATTGGTACCTTATGAACAATTTTCTAAAATCGTAGATTTCCCTTCAGATAATGAGTTATTAAATAACTTCTT
AATCGGATTTAAAATGATTAGAGATCAATTTAAATCAGTCCTTGAAAATGAAGGTGTTGTTGAAATTAAAGCTTTAGGTG
AAGTCTTTGATGCAAAAGTGCATCATGCAATCGAAAAAGAAAGCAATAAAGACAAACCAAATGGAACTGTATTAGAAGTA
TTACAAAATGGATATTTATTTAAAGATAGAATATTAAGACCAGCCATGGTAAAAATAAATGAATGGAGTGAAGACAATGG
CGAAGACAAATAA

Upstream 100 bases:

>100_bases
ACCTTCAAGAATGTCTTATCAAAAGGTAATCCCCTTGTTAGAGTACATTGCAGCAAATCTAGCAAAACTATATAAAGACA
ATAATAAGGAGACTTAGAAG

Downstream 100 bases:

>100_bases
AATTATTGGAATTGACTTAGGAACTACAAACTCAGTTGTATCAGTGATGGAAGGTAACGAAGCAAAAGTTATCCCAAATG
CTGAAGGTGGACGTACAACA

Product: molecular chaperone GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 190; Mature: 189

Protein sequence:

>190_residues
MADKEKDAVIVDETEHVDVDSKESKKEKKTKQQQKIEFLEAEVKELNDKYLRTLAEAENFKKRIQAEKIMDRKYAASSFA
TELLVPYEQFSKIVDFPSDNELLNNFLIGFKMIRDQFKSVLENEGVVEIKALGEVFDAKVHHAIEKESNKDKPNGTVLEV
LQNGYLFKDRILRPAMVKINEWSEDNGEDK

Sequences:

>Translated_190_residues
MADKEKDAVIVDETEHVDVDSKESKKEKKTKQQQKIEFLEAEVKELNDKYLRTLAEAENFKKRIQAEKIMDRKYAASSFA
TELLVPYEQFSKIVDFPSDNELLNNFLIGFKMIRDQFKSVLENEGVVEIKALGEVFDAKVHHAIEKESNKDKPNGTVLEV
LQNGYLFKDRILRPAMVKINEWSEDNGEDK
>Mature_189_residues
ADKEKDAVIVDETEHVDVDSKESKKEKKTKQQQKIEFLEAEVKELNDKYLRTLAEAENFKKRIQAEKIMDRKYAASSFAT
ELLVPYEQFSKIVDFPSDNELLNNFLIGFKMIRDQFKSVLENEGVVEIKALGEVFDAKVHHAIEKESNKDKPNGTVLEVL
QNGYLFKDRILRPAMVKINEWSEDNGEDK

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Escherichia coli, GI1788967, Length=176, Percent_Identity=26.7045454545455, Blast_Score=62, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324806, Length=179, Percent_Identity=30.7262569832402, Blast_Score=76, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24653432, Length=174, Percent_Identity=30.4597701149425, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_ACHLA (Q8L399)

Other databases:

- EMBL:   AF281816
- ProteinModelPortal:   Q8L399
- SMR:   Q8L399
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 22014; Mature: 21883

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADKEKDAVIVDETEHVDVDSKESKKEKKTKQQQKIEFLEAEVKELNDKYLRTLAEAENF
CCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKRIQAEKIMDRKYAASSFATELLVPYEQFSKIVDFPSDNELLNNFLIGFKMIRDQFKSV
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
LENEGVVEIKALGEVFDAKVHHAIEKESNKDKPNGTVLEVLQNGYLFKDRILRPAMVKIN
HCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHCCHHHHHHH
EWSEDNGEDK
CCCCCCCCCC
>Mature Secondary Structure 
ADKEKDAVIVDETEHVDVDSKESKKEKKTKQQQKIEFLEAEVKELNDKYLRTLAEAENF
CCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKRIQAEKIMDRKYAASSFATELLVPYEQFSKIVDFPSDNELLNNFLIGFKMIRDQFKSV
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
LENEGVVEIKALGEVFDAKVHHAIEKESNKDKPNGTVLEVLQNGYLFKDRILRPAMVKIN
HCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHCCHHHHHHH
EWSEDNGEDK
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA