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The map label for this gene is rutD [H]

Identifier: 162447369

GI number: 162447369

Start: 531240

End: 532058

Strand: Direct

Name: rutD [H]

Synonym: ACL_0507

Alternate gene names: 162447369

Gene position: 531240-532058 (Clockwise)

Preceding gene: 162447368

Following gene: 162447370

Centisome position: 35.49

GC content: 31.62

Gene sequence:

>819_bases
ATGAGCTACTTTAATTATTTAGGTAAAAAAATATATTACGATGTTGCTGGAAGTGGTCAACCGGTTGTTATTTTAAATGG
CATCATGATGTCTACTAAAAGTTGGGAACCTTTTAAAGAAAGTTTAAGTGAACACTTTCAATTTATTAGGGTAGACTTTT
TAGATCAAGGACAAAGTGATTCTTATGAAACAAACTATACTCAAAGTGTACAAGTAGACCTAGTTAAAGCATTATTAGAT
CACTTAGATATAAATAAGGTAGATCTAGTAGGTATTAGCTATGGTGGTGAAGTTGCATTACAGTTTGCAATTAAATATGG
TCACTATCTCGATAGATTGATTGTGTATAACTCAGTAGCATATACTACATATACACTTGCTGAAACTGGTCATCTTTGGA
ATCAATTTGCTAAGGAAAAGGATGCGCATAAATACTATGAAGCAACTATTCCAGTGATTTATTCTAAGGAGTTTAACAAA
ACTAAAAAAGAATGGATGGCCGCTAGAAAAAAATTCTTAACAGAAAATACTTTTAGTGACGAGAAATTCTTAAACCGAAT
GATTAGACTAACCAATTCTGCAGAAAGCTACGATGTAAGACATGAACTTGATAAGATCAAGGTACCTGTCATGATTGTAG
GTGCAGAAGAAGATTATTTAACACCACTAGTTCATCAAAGAATTTTAAATAATAATTTACCAAACTCTAGACTTGTGATA
CTACCAGGTGTTGGTCATGCTTCCATGTATGAAGTACCTGAAGTGTTTACAACAATCTTAATCGGATTTTTAAAGACTAA
ATTAGAATTTACGATATAA

Upstream 100 bases:

>100_bases
ATTCAAGAAAGAACCAAACTACTTATCTCCATCGCGCATCCTAAATTTAGAGAACAATTACTAAAAGAAGCTAAAGAATT
AATGTACATTGGTCAAGATT

Downstream 100 bases:

>100_bases
CACTAGGAGGGGATATTAGTGAAATTAAAACAAGTTGAACTTAAAAATGGTGAAACCATAAGCTATTATGAACAAGGAAA
TTCTAAGGAGACATTACTGC

Product: alpha/beta hydrolase superfamily protein

Products: 3-oxoadipate

Alternate protein names: Aminohydrolase [H]

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MSYFNYLGKKIYYDVAGSGQPVVILNGIMMSTKSWEPFKESLSEHFQFIRVDFLDQGQSDSYETNYTQSVQVDLVKALLD
HLDINKVDLVGISYGGEVALQFAIKYGHYLDRLIVYNSVAYTTYTLAETGHLWNQFAKEKDAHKYYEATIPVIYSKEFNK
TKKEWMAARKKFLTENTFSDEKFLNRMIRLTNSAESYDVRHELDKIKVPVMIVGAEEDYLTPLVHQRILNNNLPNSRLVI
LPGVGHASMYEVPEVFTTILIGFLKTKLEFTI

Sequences:

>Translated_272_residues
MSYFNYLGKKIYYDVAGSGQPVVILNGIMMSTKSWEPFKESLSEHFQFIRVDFLDQGQSDSYETNYTQSVQVDLVKALLD
HLDINKVDLVGISYGGEVALQFAIKYGHYLDRLIVYNSVAYTTYTLAETGHLWNQFAKEKDAHKYYEATIPVIYSKEFNK
TKKEWMAARKKFLTENTFSDEKFLNRMIRLTNSAESYDVRHELDKIKVPVMIVGAEEDYLTPLVHQRILNNNLPNSRLVI
LPGVGHASMYEVPEVFTTILIGFLKTKLEFTI
>Mature_271_residues
SYFNYLGKKIYYDVAGSGQPVVILNGIMMSTKSWEPFKESLSEHFQFIRVDFLDQGQSDSYETNYTQSVQVDLVKALLDH
LDINKVDLVGISYGGEVALQFAIKYGHYLDRLIVYNSVAYTTYTLAETGHLWNQFAKEKDAHKYYEATIPVIYSKEFNKT
KKEWMAARKKFLTENTFSDEKFLNRMIRLTNSAESYDVRHELDKIKVPVMIVGAEEDYLTPLVHQRILNNNLPNSRLVIL
PGVGHASMYEVPEVFTTILIGFLKTKLEFTI

Specific function: May increase the rate of spontaneous hydrolysis of aminoacrylate to malonic semialdehyde. Required to remove a toxic intermediate produce in the pyrimidine nitrogen degradation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Hydrolase RutD family [H]

Homologues:

Organism=Escherichia coli, GI1787244, Length=242, Percent_Identity=26.0330578512397, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR019913 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.1.1.24

Molecular weight: Translated: 31389; Mature: 31258

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYFNYLGKKIYYDVAGSGQPVVILNGIMMSTKSWEPFKESLSEHFQFIRVDFLDQGQSD
CCHHHHHCCEEEEEECCCCCCEEEEECEEECCCCCCHHHHHHHHHHHEEEEEECCCCCCC
SYETNYTQSVQVDLVKALLDHLDINKVDLVGISYGGEVALQFAIKYGHYLDRLIVYNSVA
CCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEHHHHHHHHHHHHHHHHHCCHH
YTTYTLAETGHLWNQFAKEKDAHKYYEATIPVIYSKEFNKTKKEWMAARKKFLTENTFSD
HEEEEHHHHHHHHHHHHHHHHHHHHHHEECCEEECCCCCHHHHHHHHHHHHHHHCCCCCH
EKFLNRMIRLTNSAESYDVRHELDKIKVPVMIVGAEEDYLTPLVHQRILNNNLPNSRLVI
HHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCEEEE
LPGVGHASMYEVPEVFTTILIGFLKTKLEFTI
EECCCCCHHHHHHHHHHHHHHHHHHHHHEECC
>Mature Secondary Structure 
SYFNYLGKKIYYDVAGSGQPVVILNGIMMSTKSWEPFKESLSEHFQFIRVDFLDQGQSD
CHHHHHCCEEEEEECCCCCCEEEEECEEECCCCCCHHHHHHHHHHHEEEEEECCCCCCC
SYETNYTQSVQVDLVKALLDHLDINKVDLVGISYGGEVALQFAIKYGHYLDRLIVYNSVA
CCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEHHHHHHHHHHHHHHHHHCCHH
YTTYTLAETGHLWNQFAKEKDAHKYYEATIPVIYSKEFNKTKKEWMAARKKFLTENTFSD
HEEEEHHHHHHHHHHHHHHHHHHHHHHEECCEEECCCCCHHHHHHHHHHHHHHHCCCCCH
EKFLNRMIRLTNSAESYDVRHELDKIKVPVMIVGAEEDYLTPLVHQRILNNNLPNSRLVI
HHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCEEEE
LPGVGHASMYEVPEVFTTILIGFLKTKLEFTI
EECCCCCHHHHHHHHHHHHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-oxoadipate enol-lactone; H2O

Specific reaction: 3-oxoadipate enol-lactone + H2O = 3-oxoadipate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA