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The map label for this gene is birA [H]

Identifier: 162447329

GI number: 162447329

Start: 490884

End: 491939

Strand: Direct

Name: birA [H]

Synonym: ACL_0466

Alternate gene names: 162447329

Gene position: 490884-491939 (Clockwise)

Preceding gene: 162447328

Following gene: 162447330

Centisome position: 32.79

GC content: 30.3

Gene sequence:

>1056_bases
ATGGTTAAAGTAAAATTAATAGAGTTTAAAAAAATACCCTCAACCAGTGACTATCTAAAACAAAATTATGAAAAACTGGA
CTCTTTTACCTTTGTAAGAACAGATTATCAAACTAAAGGTAGAGGTCAATTCGAACGTCAATGGATGAGTGCGAATGGAC
GTAATTTATTACTTTCGTTTATGATTAAAGATGTACCGATAAATCAGCTGATTACAATCAAAGAATGGGTGAAAAGTAGT
ATCTTTTCAACACTAGGTTCATTAGGTTTAGATGTATATTTCAAAGAGCCTAATGACGTCTATTGTCATCAAAAAAAGTT
ATGCGGAATATTGATGGAAACAAAAGGATCGGGCGATAAATTTGATTATGTCATCGTAGGTATTGGTTTAAATGTGAATC
AATTTATATTTCACAAGTTTAAGGCAACGTCCATATTTTTAGAAACAAAGAAAACTCAAAATGTAAGAAAGATTATGTCT
AAACTAATGACTAATCTTTTAGAAAGTTCATTTTTAAGGTGCAATATGACGATTAAAAGAATTATTATTATATCTATGTT
TGCAGCTTTAATTGCTGTCTCTACATTTATGAATGTACCAGTACCACCTGTAAGTTTTACCCTACAAACGTTAATGATTG
TGCTTACGGGATTATTATTAACACCTCTAGATGCATTTTTAGCAGTATTAGTCTATTTAACTGCTGGTGCATTTGGTATG
CCTATTTTTACAACTGGCGGTGGATTTCAAAGTTTTGTAGCACCGACAGGGGGATTTTTATTATCATTTTTAGTTGTAGC
TCCTGGTATATCCTTATTTAAATCTAAAAGTAAAAATATCTTACAAGATGGAATCGTCTTAATGATATTTGGCTTCTTGA
TTGTTTATTTATTTGGTATAGCAATCTTTATGTATGCGACAAGTTTGGATTTTATATATACCATAGGTGTTTTTATACCA
TACTATATTTGGGATATAGCAAAACTTATATTTGCTTATGTAGTCTATTACTACATGCCGCAAGCAATCATAGATAAACA
TTTAAAAGGGATATAA

Upstream 100 bases:

>100_bases
TAACCCGTATTCGTGGTGCTTTTGGTGTGGGTAGCGCTAAAGCATGGGTAGAAGGCGAATTAGTATGTGAAGCTGATATT
AGTTTTGCTATTGGCTGATC

Downstream 100 bases:

>100_bases
AATCCCTTTTTATTATATAAACCTTAAGCTAAATTACCACAATTTACCAAATGAAGCATTGCTTCTATAGAAATAATAAA
AGTTTGCTATAATCTATGAA

Product: biotin-[acetyl-CoA-carboxylase] ligase

Products: AMP; diphosphate; [acetyl-CoA:carbon-dioxideligase (ADP-forming)] [C]

Alternate protein names: NA

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MVKVKLIEFKKIPSTSDYLKQNYEKLDSFTFVRTDYQTKGRGQFERQWMSANGRNLLLSFMIKDVPINQLITIKEWVKSS
IFSTLGSLGLDVYFKEPNDVYCHQKKLCGILMETKGSGDKFDYVIVGIGLNVNQFIFHKFKATSIFLETKKTQNVRKIMS
KLMTNLLESSFLRCNMTIKRIIIISMFAALIAVSTFMNVPVPPVSFTLQTLMIVLTGLLLTPLDAFLAVLVYLTAGAFGM
PIFTTGGGFQSFVAPTGGFLLSFLVVAPGISLFKSKSKNILQDGIVLMIFGFLIVYLFGIAIFMYATSLDFIYTIGVFIP
YYIWDIAKLIFAYVVYYYMPQAIIDKHLKGI

Sequences:

>Translated_351_residues
MVKVKLIEFKKIPSTSDYLKQNYEKLDSFTFVRTDYQTKGRGQFERQWMSANGRNLLLSFMIKDVPINQLITIKEWVKSS
IFSTLGSLGLDVYFKEPNDVYCHQKKLCGILMETKGSGDKFDYVIVGIGLNVNQFIFHKFKATSIFLETKKTQNVRKIMS
KLMTNLLESSFLRCNMTIKRIIIISMFAALIAVSTFMNVPVPPVSFTLQTLMIVLTGLLLTPLDAFLAVLVYLTAGAFGM
PIFTTGGGFQSFVAPTGGFLLSFLVVAPGISLFKSKSKNILQDGIVLMIFGFLIVYLFGIAIFMYATSLDFIYTIGVFIP
YYIWDIAKLIFAYVVYYYMPQAIIDKHLKGI
>Mature_351_residues
MVKVKLIEFKKIPSTSDYLKQNYEKLDSFTFVRTDYQTKGRGQFERQWMSANGRNLLLSFMIKDVPINQLITIKEWVKSS
IFSTLGSLGLDVYFKEPNDVYCHQKKLCGILMETKGSGDKFDYVIVGIGLNVNQFIFHKFKATSIFLETKKTQNVRKIMS
KLMTNLLESSFLRCNMTIKRIIIISMFAALIAVSTFMNVPVPPVSFTLQTLMIVLTGLLLTPLDAFLAVLVYLTAGAFGM
PIFTTGGGFQSFVAPTGGFLLSFLVVAPGISLFKSKSKNILQDGIVLMIFGFLIVYLFGIAIFMYATSLDFIYTIGVFIP
YYIWDIAKLIFAYVVYYYMPQAIIDKHLKGI

Specific function: Probable biotin transporter [H]

COG id: COG1268

COG function: function code R; Uncharacterized conserved protein

Gene ontology:
GO:0003824: Biotin-[acetyl-CoA-carboxylase] ligase
GO:0004077: Biotin-[acetyl-CoA-carboxylase] ligase
GO:0006464: Biotin-[acetyl-CoA-carboxylase] ligase
GO:0016874: Biotin-[acetyl-CoA-carboxylase] ligase

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BioY family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003784 [H]

Pfam domain/function: PF02632 BioY [H]

EC number: 6.3.4.15 [C]

Molecular weight: Translated: 39717; Mature: 39717

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKVKLIEFKKIPSTSDYLKQNYEKLDSFTFVRTDYQTKGRGQFERQWMSANGRNLLLSF
CEEEEEEEECCCCCCHHHHHHHHHHHHCEEEEEECCCCCCCCHHHHHHHCCCCCHHHHHH
MIKDVPINQLITIKEWVKSSIFSTLGSLGLDVYFKEPNDVYCHQKKLCGILMETKGSGDK
HHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCC
FDYVIVGIGLNVNQFIFHKFKATSIFLETKKTQNVRKIMSKLMTNLLESSFLRCNMTIKR
EEEEEEEECCCHHHHHHHHHHHHEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IIIISMFAALIAVSTFMNVPVPPVSFTLQTLMIVLTGLLLTPLDAFLAVLVYLTAGAFGM
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PIFTTGGGFQSFVAPTGGFLLSFLVVAPGISLFKSKSKNILQDGIVLMIFGFLIVYLFGI
CEEECCCCHHHHHCCCHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
AIFMYATSLDFIYTIGVFIPYYIWDIAKLIFAYVVYYYMPQAIIDKHLKGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCC
>Mature Secondary Structure
MVKVKLIEFKKIPSTSDYLKQNYEKLDSFTFVRTDYQTKGRGQFERQWMSANGRNLLLSF
CEEEEEEEECCCCCCHHHHHHHHHHHHCEEEEEECCCCCCCCHHHHHHHCCCCCHHHHHH
MIKDVPINQLITIKEWVKSSIFSTLGSLGLDVYFKEPNDVYCHQKKLCGILMETKGSGDK
HHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCC
FDYVIVGIGLNVNQFIFHKFKATSIFLETKKTQNVRKIMSKLMTNLLESSFLRCNMTIKR
EEEEEEEECCCHHHHHHHHHHHHEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IIIISMFAALIAVSTFMNVPVPPVSFTLQTLMIVLTGLLLTPLDAFLAVLVYLTAGAFGM
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PIFTTGGGFQSFVAPTGGFLLSFLVVAPGISLFKSKSKNILQDGIVLMIFGFLIVYLFGI
CEEECCCCHHHHHCCCHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
AIFMYATSLDFIYTIGVFIPYYIWDIAKLIFAYVVYYYMPQAIIDKHLKGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; biotin; apo-[acetyl-CoA:carbon-dioxide ligase(ADP-forming)] [C]

Specific reaction: ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase(ADP-forming)] = AMP + diphosphate + [acetyl-CoA:carbon-dioxideligase (ADP-forming)] [C]

General reaction: Amination; Carboxylic acid formation (carboxamide formation [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9665876 [H]