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The map label for this gene is clcA [H]

Identifier: 162447308

GI number: 162447308

Start: 465089

End: 466468

Strand: Direct

Name: clcA [H]

Synonym: ACL_0444

Alternate gene names: 162447308

Gene position: 465089-466468 (Clockwise)

Preceding gene: 162447307

Following gene: 162447309

Centisome position: 31.07

GC content: 32.25

Gene sequence:

>1380_bases
GTGTCTCTACTTATTAAATGGGAACCTCATGCAGGTGGTGGAGCGATTTCAAGAGCGGCAGGGTTTGTTCGCGGAATTCT
TTTATTTAATTGGTTAAAAACATTGATTTCAACATTCATTTCAGCTCAGTTAGTATTCTTTGCTGGATTACCATTTGGTA
TTGAAGGTCCTTCTGTAATCATGGGAACGGCAATTTCAGGTGGCGTAGATGATCTTGGAAAAGCACATCCAGCACATAGA
AAATATTTACTTACTGCTGGTGCATCAGCTGGATTTTCAATCGCTACAGGCTCGATTTTTGCAGGAACGATATTTACATT
AGAAGAGATGCATAAGAAGTTCTCCTTTATGCTTTTATCTGTTGCTATGAGTGGAGCTGTTTTTGCATCTGTAACTATGA
GAATACTCGATTATGTATTCGGTAACCATACTGCATTTTTCTCACTGACAAATGAATTTAAAAATATACCATTTGAATTT
ATTTGGTTAGCACCTTTATTGGGCATTGGAGCAGGTTTATTAGCTGCAGGGTTTAATTTCCTAATTGACTTTTTTGGGAA
TATGAGTGCTTATAAATTCAAAAAAGTTCCACTTTTTGTGAAATTAACAATAAATTTCTTGCTTGTAGGTTTTGTTGGTT
TATTTGTTATTGAAACCATTGGAAATGGCCATCATAGTATTATAGAACCGTTATTTGATAGACAGTTTAGTTTTGGTATG
ATTGTATTACTCATCGTTTTAAAAATTATTACAATTGTTGTATCATCAAGTGGTACAACAGGTGGGTTATTTATTCCTGT
ATTAGTGATTGGAGCTTTATATGCAGGTATATTTAATGAAGTGTCTATTTTAATTGGATTAAATAGTGAATATACAGCTG
CAATTGTTTTAATTGGCATGAGTGTATTTTTTAGTACCTCCATGCAAGCACCTATTACAACCATAGCATTTATTATTGAA
GCATCCCTTCATCCAGAAACAATCATATTTATGATGGTTGCTATTTTAGCAGGTATATTAATTGCAGAATTATTACATAT
GAAACCTTTAAATGAAATTGCTTTAAAAAGATTTATGAAGATTCAAGATCAAAATAGAGTCTGGAATTTATACAATGCTA
AAGCAACCATTCAAGAAAATGCGTTTGTTATAGGGAAAACAGTAAGAGATATATTATGGCCAGCAAATACCCTCATTAAA
GAGTTGATCCAAGAAAATCAACAAAATGCTACCAATATTGCTGTACATGGTGGAGACCGTGTATTAACATATAGAGATCA
AATTCTATTTCAAATTCAATCATTTGATATTGAAAGAACATTAAAAGAATTACAGGATTTAATCGGTATTCAAGAAGTAG
AAATAGAATTGATTAGATAA

Upstream 100 bases:

>100_bases
GTTTATTATTAGAACTTACCTATTCGATATACTTATCAGTTCAGTCAAATCTATGGTATTTACCATTTTTTGAATAGGTT
TAGTTGTTCTTGCTTATTTA

Downstream 100 bases:

>100_bases
GTTGTGAAATACTAGTAAGTTGATACATATATTCTAACAATAAGTAAAAATACATTATTATCTCCATTATATAGAGTCAT
TCACGTTTTCGGAGATAAGG

Product: voltage-gated chloride ion channel protein

Products: Cl Ion [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MSLLIKWEPHAGGGAISRAAGFVRGILLFNWLKTLISTFISAQLVFFAGLPFGIEGPSVIMGTAISGGVDDLGKAHPAHR
KYLLTAGASAGFSIATGSIFAGTIFTLEEMHKKFSFMLLSVAMSGAVFASVTMRILDYVFGNHTAFFSLTNEFKNIPFEF
IWLAPLLGIGAGLLAAGFNFLIDFFGNMSAYKFKKVPLFVKLTINFLLVGFVGLFVIETIGNGHHSIIEPLFDRQFSFGM
IVLLIVLKIITIVVSSSGTTGGLFIPVLVIGALYAGIFNEVSILIGLNSEYTAAIVLIGMSVFFSTSMQAPITTIAFIIE
ASLHPETIIFMMVAILAGILIAELLHMKPLNEIALKRFMKIQDQNRVWNLYNAKATIQENAFVIGKTVRDILWPANTLIK
ELIQENQQNATNIAVHGGDRVLTYRDQILFQIQSFDIERTLKELQDLIGIQEVEIELIR

Sequences:

>Translated_459_residues
MSLLIKWEPHAGGGAISRAAGFVRGILLFNWLKTLISTFISAQLVFFAGLPFGIEGPSVIMGTAISGGVDDLGKAHPAHR
KYLLTAGASAGFSIATGSIFAGTIFTLEEMHKKFSFMLLSVAMSGAVFASVTMRILDYVFGNHTAFFSLTNEFKNIPFEF
IWLAPLLGIGAGLLAAGFNFLIDFFGNMSAYKFKKVPLFVKLTINFLLVGFVGLFVIETIGNGHHSIIEPLFDRQFSFGM
IVLLIVLKIITIVVSSSGTTGGLFIPVLVIGALYAGIFNEVSILIGLNSEYTAAIVLIGMSVFFSTSMQAPITTIAFIIE
ASLHPETIIFMMVAILAGILIAELLHMKPLNEIALKRFMKIQDQNRVWNLYNAKATIQENAFVIGKTVRDILWPANTLIK
ELIQENQQNATNIAVHGGDRVLTYRDQILFQIQSFDIERTLKELQDLIGIQEVEIELIR
>Mature_458_residues
SLLIKWEPHAGGGAISRAAGFVRGILLFNWLKTLISTFISAQLVFFAGLPFGIEGPSVIMGTAISGGVDDLGKAHPAHRK
YLLTAGASAGFSIATGSIFAGTIFTLEEMHKKFSFMLLSVAMSGAVFASVTMRILDYVFGNHTAFFSLTNEFKNIPFEFI
WLAPLLGIGAGLLAAGFNFLIDFFGNMSAYKFKKVPLFVKLTINFLLVGFVGLFVIETIGNGHHSIIEPLFDRQFSFGMI
VLLIVLKIITIVVSSSGTTGGLFIPVLVIGALYAGIFNEVSILIGLNSEYTAAIVLIGMSVFFSTSMQAPITTIAFIIEA
SLHPETIIFMMVAILAGILIAELLHMKPLNEIALKRFMKIQDQNRVWNLYNAKATIQENAFVIGKTVRDILWPANTLIKE
LIQENQQNATNIAVHGGDRVLTYRDQILFQIQSFDIERTLKELQDLIGIQEVEIELIR

Specific function: Proton-coupled chloride transporter. Functions as antiport system and exchanges two chloride ions for 1 proton. Probably acts as an electrical shunt for an outwardly-directed proton pump that is linked to amino acid decarboxylation, as part of the extreme

COG id: COG0038

COG function: function code P; Chloride channel protein EriC

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chloride channel (TC 2.A.49) family. ClcA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786350, Length=383, Percent_Identity=26.8929503916449, Blast_Score=96, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014743
- InterPro:   IPR001807 [H]

Pfam domain/function: PF00654 Voltage_CLC [H]

EC number: NA

Molecular weight: Translated: 50368; Mature: 50237

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: PS00107 PROTEIN_KINASE_ATP ; PS00659 GLYCOSYL_HYDROL_F5 ; PS00211 ABC_TRANSPORTER_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLLIKWEPHAGGGAISRAAGFVRGILLFNWLKTLISTFISAQLVFFAGLPFGIEGPSVI
CCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEE
MGTAISGGVDDLGKAHPAHRKYLLTAGASAGFSIATGSIFAGTIFTLEEMHKKFSFMLLS
EECCCCCCHHHHHCCCCCCCEEEEEECCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHH
VAMSGAVFASVTMRILDYVFGNHTAFFSLTNEFKNIPFEFIWLAPLLGIGAGLLAAGFNF
HHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
LIDFFGNMSAYKFKKVPLFVKLTINFLLVGFVGLFVIETIGNGHHSIIEPLFDRQFSFGM
HHHHHCCCCCCEEECCCEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHH
IVLLIVLKIITIVVSSSGTTGGLFIPVLVIGALYAGIFNEVSILIGLNSEYTAAIVLIGM
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCEEEEEECCCCHHHHHHHHHH
SVFFSTSMQAPITTIAFIIEASLHPETIIFMMVAILAGILIAELLHMKPLNEIALKRFMK
HHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
IQDQNRVWNLYNAKATIQENAFVIGKTVRDILWPANTLIKELIQENQQNATNIAVHGGDR
HCCCCCEEEEECCEEEECCCCEEEHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEECCCE
VLTYRDQILFQIQSFDIERTLKELQDLIGIQEVEIELIR
EEEEHHHHHHHHHHCCHHHHHHHHHHHHCCEEHEEEEEC
>Mature Secondary Structure 
SLLIKWEPHAGGGAISRAAGFVRGILLFNWLKTLISTFISAQLVFFAGLPFGIEGPSVI
CEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEE
MGTAISGGVDDLGKAHPAHRKYLLTAGASAGFSIATGSIFAGTIFTLEEMHKKFSFMLLS
EECCCCCCHHHHHCCCCCCCEEEEEECCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHH
VAMSGAVFASVTMRILDYVFGNHTAFFSLTNEFKNIPFEFIWLAPLLGIGAGLLAAGFNF
HHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
LIDFFGNMSAYKFKKVPLFVKLTINFLLVGFVGLFVIETIGNGHHSIIEPLFDRQFSFGM
HHHHHCCCCCCEEECCCEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHH
IVLLIVLKIITIVVSSSGTTGGLFIPVLVIGALYAGIFNEVSILIGLNSEYTAAIVLIGM
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCEEEEEECCCCHHHHHHHHHH
SVFFSTSMQAPITTIAFIIEASLHPETIIFMMVAILAGILIAELLHMKPLNEIALKRFMK
HHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
IQDQNRVWNLYNAKATIQENAFVIGKTVRDILWPANTLIKELIQENQQNATNIAVHGGDR
HCCCCCEEEEECCEEEECCCCEEEHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEECCCE
VLTYRDQILFQIQSFDIERTLKELQDLIGIQEVEIELIR
EEEEHHHHHHHHHHCCHHHHHHHHHHHHCCEEHEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Cl Ion [Periplasm] [C]

Specific reaction: Cl Ion [Periplasm] = Cl Ion [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA