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The map label for this gene is trmA [C]

Identifier: 162447304

GI number: 162447304

Start: 461844

End: 463136

Strand: Direct

Name: trmA [C]

Synonym: ACL_0440

Alternate gene names: 162447304

Gene position: 461844-463136 (Clockwise)

Preceding gene: 162447303

Following gene: 162447305

Centisome position: 30.85

GC content: 29.62

Gene sequence:

>1293_bases
ATGGTGGAAGTATATTTAATAACTTCTATAGCTAAAATAATGAGATTAAAAGTAAAAACAAATGATCTTGATTACCAAGG
TCAAGGTGTAACAAGAATAGATGATAAAGTAACCTTTATAAAAGGTATGTTTGATGATGAAGTAGGCATCATAGAAATCA
CAAAATCAAAAAAGAATTTCCAACAAGCAAAACTTGTAGAACTTATTCAAAAAAATAAACAACGTGTATCTGAAAACTCT
TTTGACTATGCACCGTTTTTTGGTCTATCCTTAAAAGCAGAATGCCTATGGCAACAAAAGATTACTAAAGAAACGATTAA
AAAAATAACCAATTTAGATGTTGAAGTAGAAGATACTATCACAGATGGTAAGAAACTAAACTACAGAAATAAAATCACTT
TACATGTGAAACATATTGGTGGTTCATTAAAGATTGGTACCTATGAAGAAAACTCTAATTATCTTGAACCGATTGAAACA
CATCATCTTGCACTACCTGTCATAAATGATAAAATTACAGTTTTAAATAAATGGCTCAAAACCTTTAAATTAGAAACGAA
TGATATTAAAAACTTTACCCTTAGAACAAACGGTAAAGATGTGATGCTTATCATTGGTATAAAACTTAAGTTTGAAGGTA
CAGAAAGCATCATAGATGAACTTAAAAAGCACTTTGAATCCATCTACCTAAATATTGAAAAAAGAAGTTTTGAAAACCTA
TCAGATGAATCGATTCATGTTTATGGTCTAGAGACCTTAAAGATGACATTTAATCACTTATCATTTGAAATAGGACCTGA
AAGTTTCTTCCAGGTAAATAAAGATGTAGCAATTTTAATGTATGATGAAATTAAGAAACTTACCCAAAATCACACAGTCA
TTGATGCCTATGCTGGTATGGCAAGTATTGGTCAATATATTGATGCTAAAAAGGTATACGCAATTGAATCTAATCTAGAT
TCAATTATTCAAGCTAAGAAGATATTAAAGGTAAATCAAGTAGACCATGTAGAGTTGATTCATGGACAAGTAGAAGAAAA
ACTAGAAGCATACATTAAACATGCAGATGCTATAGTGTTTGATCCACCAAGAAGTGGATTAACGGAAAGTATTATTGAAC
TAGTAAAACTCAATCAGATTAAGACGATTGTATATGTGTCTTGTGATTTAAAATCACTAGCAAGAGATATCAATGGCTTA
AAAGATTTATATGATATTAAAAAAATCATTCCAGTTAGAATGTTTCCGTCCACAATTCATATGGAAACAATTACCTTGCT
TTCGTTAAAATAG

Upstream 100 bases:

>100_bases
CTATTTATAATTTTATTTATCATGAATACAAACTTGATATTTAACTAATATGGTCTCAATATGCTAAAATATGATGTGTG
ATAATTATTTGAAATAAGAA

Downstream 100 bases:

>100_bases
CACTTATTGGACATCTATCACTTATCTGAAAATAGCATAAAAACATCAAACTTTTAATTTAGAAGGTTGGTGTTCTTTAT
TTTAGTACCACTAATATTAT

Product: tRNA (uracil-5-)-methyltransferase, SAM dependent

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MVEVYLITSIAKIMRLKVKTNDLDYQGQGVTRIDDKVTFIKGMFDDEVGIIEITKSKKNFQQAKLVELIQKNKQRVSENS
FDYAPFFGLSLKAECLWQQKITKETIKKITNLDVEVEDTITDGKKLNYRNKITLHVKHIGGSLKIGTYEENSNYLEPIET
HHLALPVINDKITVLNKWLKTFKLETNDIKNFTLRTNGKDVMLIIGIKLKFEGTESIIDELKKHFESIYLNIEKRSFENL
SDESIHVYGLETLKMTFNHLSFEIGPESFFQVNKDVAILMYDEIKKLTQNHTVIDAYAGMASIGQYIDAKKVYAIESNLD
SIIQAKKILKVNQVDHVELIHGQVEEKLEAYIKHADAIVFDPPRSGLTESIIELVKLNQIKTIVYVSCDLKSLARDINGL
KDLYDIKKIIPVRMFPSTIHMETITLLSLK

Sequences:

>Translated_430_residues
MVEVYLITSIAKIMRLKVKTNDLDYQGQGVTRIDDKVTFIKGMFDDEVGIIEITKSKKNFQQAKLVELIQKNKQRVSENS
FDYAPFFGLSLKAECLWQQKITKETIKKITNLDVEVEDTITDGKKLNYRNKITLHVKHIGGSLKIGTYEENSNYLEPIET
HHLALPVINDKITVLNKWLKTFKLETNDIKNFTLRTNGKDVMLIIGIKLKFEGTESIIDELKKHFESIYLNIEKRSFENL
SDESIHVYGLETLKMTFNHLSFEIGPESFFQVNKDVAILMYDEIKKLTQNHTVIDAYAGMASIGQYIDAKKVYAIESNLD
SIIQAKKILKVNQVDHVELIHGQVEEKLEAYIKHADAIVFDPPRSGLTESIIELVKLNQIKTIVYVSCDLKSLARDINGL
KDLYDIKKIIPVRMFPSTIHMETITLLSLK
>Mature_430_residues
MVEVYLITSIAKIMRLKVKTNDLDYQGQGVTRIDDKVTFIKGMFDDEVGIIEITKSKKNFQQAKLVELIQKNKQRVSENS
FDYAPFFGLSLKAECLWQQKITKETIKKITNLDVEVEDTITDGKKLNYRNKITLHVKHIGGSLKIGTYEENSNYLEPIET
HHLALPVINDKITVLNKWLKTFKLETNDIKNFTLRTNGKDVMLIIGIKLKFEGTESIIDELKKHFESIYLNIEKRSFENL
SDESIHVYGLETLKMTFNHLSFEIGPESFFQVNKDVAILMYDEIKKLTQNHTVIDAYAGMASIGQYIDAKKVYAIESNLD
SIIQAKKILKVNQVDHVELIHGQVEEKLEAYIKHADAIVFDPPRSGLTESIIELVKLNQIKTIVYVSCDLKSLARDINGL
KDLYDIKKIIPVRMFPSTIHMETITLLSLK

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:
GO:0001510: tRNA (Uracil-5-)-methyltransferase, SAM dependent
GO:0003723: tRNA (Uracil-5-)-methyltransferase, SAM dependent
GO:0006396: tRNA (Uracil-5-)-methyltransferase, SAM dependent
GO:0008168: tRNA (Uracil-5-)-methyltransferase, SAM dependent
GO:0008173: tRNA (Uracil-5-)-methyltransferase, SAM dependent
GO:0009021: tRNA (Uracil-5-)-methyltransferase, SAM dependent
GO:0016740: tRNA (Uracil-5-)-methyltransferase, SAM dependent
GO:0032259: tRNA (Uracil-5-)-methyltransferase, SAM dependent

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=279, Percent_Identity=25.8064516129032, Blast_Score=76, Evalue=6e-14,
Organism=Homo sapiens, GI51173878, Length=279, Percent_Identity=25.8064516129032, Blast_Score=76, Evalue=6e-14,
Organism=Escherichia coli, GI1789148, Length=431, Percent_Identity=24.3619489559165, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1787083, Length=215, Percent_Identity=25.1162790697674, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24666579, Length=269, Percent_Identity=25.6505576208178, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: NA

Molecular weight: Translated: 49347; Mature: 49347

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEVYLITSIAKIMRLKVKTNDLDYQGQGVTRIDDKVTFIKGMFDDEVGIIEITKSKKNF
CEEEEEHHHHHHHHEEEEECCCCCCCCCCCEEECCCEEEEECCCCCCCCEEEEECCCCCH
QQAKLVELIQKNKQRVSENSFDYAPFFGLSLKAECLWQQKITKETIKKITNLDVEVEDTI
HHHHHHHHHHHHHHHHCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCC
TDGKKLNYRNKITLHVKHIGGSLKIGTYEENSNYLEPIETHHLALPVINDKITVLNKWLK
CCCCEECCCCEEEEEEEECCCEEEEEEEECCCCCCCCCCCCEEEEEEECCHHHHHHHHHH
TFKLETNDIKNFTLRTNGKDVMLIIGIKLKFEGTESIIDELKKHFESIYLNIEKRSFENL
HHCCCCCCCCEEEEEECCCEEEEEEEEEEEECCHHHHHHHHHHHHHHEEEEEEHHHCCCC
SDESIHVYGLETLKMTFNHLSFEIGPESFFQVNKDVAILMYDEIKKLTQNHTVIDAYAGM
CCCCEEEEEHHHHHHHHHHCEEEECCHHHHCCCCCEEEEEHHHHHHHHCCCEEEEHHHCH
ASIGQYIDAKKVYAIESNLDSIIQAKKILKVNQVDHVELIHGQVEEKLEAYIKHADAIVF
HHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCEEEE
DPPRSGLTESIIELVKLNQIKTIVYVSCDLKSLARDINGLKDLYDIKKIIPVRMFPSTIH
CCCCCCHHHHHHHHHHHCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCEEECCCEEE
METITLLSLK
EEEEEEEECC
>Mature Secondary Structure
MVEVYLITSIAKIMRLKVKTNDLDYQGQGVTRIDDKVTFIKGMFDDEVGIIEITKSKKNF
CEEEEEHHHHHHHHEEEEECCCCCCCCCCCEEECCCEEEEECCCCCCCCEEEEECCCCCH
QQAKLVELIQKNKQRVSENSFDYAPFFGLSLKAECLWQQKITKETIKKITNLDVEVEDTI
HHHHHHHHHHHHHHHHCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCC
TDGKKLNYRNKITLHVKHIGGSLKIGTYEENSNYLEPIETHHLALPVINDKITVLNKWLK
CCCCEECCCCEEEEEEEECCCEEEEEEEECCCCCCCCCCCCEEEEEEECCHHHHHHHHHH
TFKLETNDIKNFTLRTNGKDVMLIIGIKLKFEGTESIIDELKKHFESIYLNIEKRSFENL
HHCCCCCCCCEEEEEECCCEEEEEEEEEEEECCHHHHHHHHHHHHHHEEEEEEHHHCCCC
SDESIHVYGLETLKMTFNHLSFEIGPESFFQVNKDVAILMYDEIKKLTQNHTVIDAYAGM
CCCCEEEEEHHHHHHHHHHCEEEECCHHHHCCCCCEEEEEHHHHHHHHCCCEEEEHHHCH
ASIGQYIDAKKVYAIESNLDSIIQAKKILKVNQVDHVELIHGQVEEKLEAYIKHADAIVF
HHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCEEEE
DPPRSGLTESIIELVKLNQIKTIVYVSCDLKSLARDINGLKDLYDIKKIIPVRMFPSTIH
CCCCCCHHHHHHHHHHHCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCEEECCCEEE
METITLLSLK
EEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]