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The map label for this gene is cadA [H]

Identifier: 162447293

GI number: 162447293

Start: 447564

End: 449420

Strand: Direct

Name: cadA [H]

Synonym: ACL_0428

Alternate gene names: 162447293

Gene position: 447564-449420 (Clockwise)

Preceding gene: 162447292

Following gene: 162447294

Centisome position: 29.9

GC content: 33.55

Gene sequence:

>1857_bases
ATGGTCTCATTTATACATAAACAAAGGATGAAAATCATGATACTTTCATTCTTATTGATTGTATCAGGGTTTATAGCACA
TTATGTTTTCGATCAAAAACTGATATTTGATGTAACATTTATCATTGCATCTGTTATAGGTGTATTACCGATTGCAATAT
CTGCCTTTCAAGCATTAAAAGTGAAAGTTATTAGTATTGATTTACTTGTTACAATTGCAGTTGTTGGTGCATTTATTATT
AAGGCATATGAAGAGTCTGCAATTGTTACATTCCTATTTTTATTTGGTGCTTATTTAGAACAAAGAACACTTAATAAGAC
AAGATCAGCGATTAAGGCACTAACTGAAATGGCACCTGAAACTGCACTTAAAAAGATGGATGATGGTGAGTTTTTAGAAG
TTGGTATTGATGATGTAGAAGTAACTGATATTTTATTAGTTAAAACGGGTGCTAAGGTGCCTGTGGATGGTATTGTTTTA
AGTGGTAGTGCCTATATTAATGAAGCAAGTATTACAGGTGAAGCAGTATCTGTTAAAAAAGTTGTCGACTCCAAAGTATT
TGCAGGTACGATTTTAGATAATGGAACAATAGAAATCATTGCACAAAAAGTAGGAGAAGATACTACGTTTGGTAAAATCA
TAGAACTTGTTGAAGAAGCACAAGATTCGAAGTCTGAGGCTGAAAAGTTTATTGATAGGTTTGCTACATGGTATACGCCG
TTTGTTTTAGTACTTGCGCTTATTGTTTGGGTGATTACACAAGATGTAGCATTAGCTATTACAATACTCGTACTAGGTTG
CCCGGGTGCGCTTGTGATTGGTGTACCAGTATCTAATGTTGCAGGTATTGGAAATGGTGCAAGACATGGTGTGTTACTAA
AGGGTAGCGAAGTGATTGGTAACTTTAGTAAGGTGGATACCATCGTTTTTGATAAAACAGGAACACTTACAATTGGACAC
CCTGTACTAAGATATGAGGCTTATTATGAATTGGATAAAAAAGATATCATACCTTATATTTACAGTGTAGAAAAACAATC
AGATCATCCGCTTGCAAAGGCTATTACCAATCATTTGAAAGATGCCAAAACACTACCTATAGACCACACAGATGTTATCA
AAGGTGGTGGGATTGTTGCAAGTATTCATGAAAGAAAAGTATTTATTGGAAATACAGTACTTATGAAAGATAATGGTATA
GAAATAAAGTCAGATATCATGGATGAAATTAGTAGATTAGAACATGAAGGACACTCCATAGTGCTTACGGCTATAGATGA
AAAGTTAGTAAGTTTAATGGGTATTCGTGATGAAGTAAGACCGAATCTTAAAAAGGATTTAAAACATATCAAAAAAATGG
GTGTTAAAAACTTAATCGTATTATCTGGTGATAATCAAGGTAGTGTTGATATCGTAGCTAAAGAGTTAGGACTTACAAAA
GCAATTGGCAACATGTTACCAGAAGATAAAGCAAACTACATTAAGGAACTAATGAATGAAGGTCATATCATCGCCTTTGT
AGGGGATGGTGTGAATGATAGTCCATCGATTGCACTTGCACATATTGGTATAGCAATGGGTGGTGGAACCGATGTTGCGA
TTGAAACATCAGATGTTGTATTAATGAATGATAACATCAATAAATTACCACATGCTTTAGGGTTAGCAAAAGCAACTAAA
CATAATATGATCCAAAATATATTAATTGCAATAGGCGTTGTAGTCGTATTATTAGTAAGTTTATTTTGGGGTGAATGGGT
GAATATGAGTATTGGTATGTTAGTTCATGAAGCAAGTATATTAGTAGTCATTTTAAATGCCATGAGACTTTTAAGATTTA
AAACAAAGATTAATTGA

Upstream 100 bases:

>100_bases
ATCATTATAAAGTACCACATGATGTATGTGAAAGTTATGAAGCCGTTTACCAAATGCTAGAAGAACTTAATAAGGCATAT
GATGAAAGTGCGGTGGTCTG

Downstream 100 bases:

>100_bases
TTTAAATCAAGGTTTATTAAAATACTTGAGTATATAATAGAAGTATAAAATAAAAATAAAGGAAGTAATAATAATGAAAA
AAACTACAATTCAATTAGAA

Product: P-type cation transporting ATPase

Products: NA

Alternate protein names: Cadmium efflux ATPase [H]

Number of amino acids: Translated: 618; Mature: 618

Protein sequence:

>618_residues
MVSFIHKQRMKIMILSFLLIVSGFIAHYVFDQKLIFDVTFIIASVIGVLPIAISAFQALKVKVISIDLLVTIAVVGAFII
KAYEESAIVTFLFLFGAYLEQRTLNKTRSAIKALTEMAPETALKKMDDGEFLEVGIDDVEVTDILLVKTGAKVPVDGIVL
SGSAYINEASITGEAVSVKKVVDSKVFAGTILDNGTIEIIAQKVGEDTTFGKIIELVEEAQDSKSEAEKFIDRFATWYTP
FVLVLALIVWVITQDVALAITILVLGCPGALVIGVPVSNVAGIGNGARHGVLLKGSEVIGNFSKVDTIVFDKTGTLTIGH
PVLRYEAYYELDKKDIIPYIYSVEKQSDHPLAKAITNHLKDAKTLPIDHTDVIKGGGIVASIHERKVFIGNTVLMKDNGI
EIKSDIMDEISRLEHEGHSIVLTAIDEKLVSLMGIRDEVRPNLKKDLKHIKKMGVKNLIVLSGDNQGSVDIVAKELGLTK
AIGNMLPEDKANYIKELMNEGHIIAFVGDGVNDSPSIALAHIGIAMGGGTDVAIETSDVVLMNDNINKLPHALGLAKATK
HNMIQNILIAIGVVVVLLVSLFWGEWVNMSIGMLVHEASILVVILNAMRLLRFKTKIN

Sequences:

>Translated_618_residues
MVSFIHKQRMKIMILSFLLIVSGFIAHYVFDQKLIFDVTFIIASVIGVLPIAISAFQALKVKVISIDLLVTIAVVGAFII
KAYEESAIVTFLFLFGAYLEQRTLNKTRSAIKALTEMAPETALKKMDDGEFLEVGIDDVEVTDILLVKTGAKVPVDGIVL
SGSAYINEASITGEAVSVKKVVDSKVFAGTILDNGTIEIIAQKVGEDTTFGKIIELVEEAQDSKSEAEKFIDRFATWYTP
FVLVLALIVWVITQDVALAITILVLGCPGALVIGVPVSNVAGIGNGARHGVLLKGSEVIGNFSKVDTIVFDKTGTLTIGH
PVLRYEAYYELDKKDIIPYIYSVEKQSDHPLAKAITNHLKDAKTLPIDHTDVIKGGGIVASIHERKVFIGNTVLMKDNGI
EIKSDIMDEISRLEHEGHSIVLTAIDEKLVSLMGIRDEVRPNLKKDLKHIKKMGVKNLIVLSGDNQGSVDIVAKELGLTK
AIGNMLPEDKANYIKELMNEGHIIAFVGDGVNDSPSIALAHIGIAMGGGTDVAIETSDVVLMNDNINKLPHALGLAKATK
HNMIQNILIAIGVVVVLLVSLFWGEWVNMSIGMLVHEASILVVILNAMRLLRFKTKIN
>Mature_618_residues
MVSFIHKQRMKIMILSFLLIVSGFIAHYVFDQKLIFDVTFIIASVIGVLPIAISAFQALKVKVISIDLLVTIAVVGAFII
KAYEESAIVTFLFLFGAYLEQRTLNKTRSAIKALTEMAPETALKKMDDGEFLEVGIDDVEVTDILLVKTGAKVPVDGIVL
SGSAYINEASITGEAVSVKKVVDSKVFAGTILDNGTIEIIAQKVGEDTTFGKIIELVEEAQDSKSEAEKFIDRFATWYTP
FVLVLALIVWVITQDVALAITILVLGCPGALVIGVPVSNVAGIGNGARHGVLLKGSEVIGNFSKVDTIVFDKTGTLTIGH
PVLRYEAYYELDKKDIIPYIYSVEKQSDHPLAKAITNHLKDAKTLPIDHTDVIKGGGIVASIHERKVFIGNTVLMKDNGI
EIKSDIMDEISRLEHEGHSIVLTAIDEKLVSLMGIRDEVRPNLKKDLKHIKKMGVKNLIVLSGDNQGSVDIVAKELGLTK
AIGNMLPEDKANYIKELMNEGHIIAFVGDGVNDSPSIALAHIGIAMGGGTDVAIETSDVVLMNDNINKLPHALGLAKATK
HNMIQNILIAIGVVVVLLVSLFWGEWVNMSIGMLVHEASILVVILNAMRLLRFKTKIN

Specific function: This electroneutral antiporter ejects one cadmium ion while accumulating two protons by an energy-dependent efflux mechanism [H]

COG id: COG2217

COG function: function code P; Cation transport ATPase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 HMA domains [H]

Homologues:

Organism=Homo sapiens, GI115529486, Length=658, Percent_Identity=31.1550151975684, Blast_Score=286, Evalue=6e-77,
Organism=Homo sapiens, GI55743071, Length=660, Percent_Identity=30.1515151515152, Blast_Score=266, Evalue=6e-71,
Organism=Homo sapiens, GI55743073, Length=508, Percent_Identity=32.6771653543307, Blast_Score=228, Evalue=2e-59,
Organism=Escherichia coli, GI1786691, Length=551, Percent_Identity=35.5716878402904, Blast_Score=319, Evalue=3e-88,
Organism=Escherichia coli, GI1789879, Length=632, Percent_Identity=32.4367088607595, Blast_Score=300, Evalue=2e-82,
Organism=Escherichia coli, GI1786914, Length=507, Percent_Identity=25.0493096646943, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI2367363, Length=569, Percent_Identity=23.0228471001757, Blast_Score=111, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17556548, Length=624, Percent_Identity=29.1666666666667, Blast_Score=206, Evalue=4e-53,
Organism=Caenorhabditis elegans, GI71997262, Length=596, Percent_Identity=22.6510067114094, Blast_Score=90, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI71997275, Length=596, Percent_Identity=22.6510067114094, Blast_Score=89, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI71997269, Length=581, Percent_Identity=22.8915662650602, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17554158, Length=188, Percent_Identity=28.1914893617021, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71988506, Length=188, Percent_Identity=28.1914893617021, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI193210130, Length=188, Percent_Identity=28.1914893617021, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320475, Length=538, Percent_Identity=28.4386617100372, Blast_Score=223, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6319772, Length=568, Percent_Identity=25.1760563380282, Blast_Score=172, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6325221, Length=531, Percent_Identity=21.8455743879473, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6321430, Length=531, Percent_Identity=21.6572504708098, Blast_Score=103, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6321271, Length=189, Percent_Identity=30.6878306878307, Blast_Score=81, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6321432, Length=189, Percent_Identity=26.4550264550265, Blast_Score=68, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6320244, Length=178, Percent_Identity=28.0898876404494, Blast_Score=67, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6320243, Length=178, Percent_Identity=28.0898876404494, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI221329854, Length=365, Percent_Identity=31.5068493150685, Blast_Score=143, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24762455, Length=212, Percent_Identity=25.4716981132075, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24762453, Length=212, Percent_Identity=25.4716981132075, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24762447, Length=212, Percent_Identity=25.4716981132075, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24762457, Length=212, Percent_Identity=25.4716981132075, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24762449, Length=212, Percent_Identity=25.4716981132075, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24762445, Length=212, Percent_Identity=25.4716981132075, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24762451, Length=212, Percent_Identity=25.4716981132075, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI17136664, Length=212, Percent_Identity=25.4716981132075, Blast_Score=73, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008250
- InterPro:   IPR001366
- InterPro:   IPR006404
- InterPro:   IPR006416
- InterPro:   IPR001757
- InterPro:   IPR018303
- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR017969
- InterPro:   IPR006121 [H]

Pfam domain/function: PF00122 E1-E2_ATPase; PF00403 HMA; PF00702 Hydrolase [H]

EC number: =3.6.3.3 [H]

Molecular weight: Translated: 67130; Mature: 67130

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00154 ATPASE_E1_E2 ; PS01229 COF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSFIHKQRMKIMILSFLLIVSGFIAHYVFDQKLIFDVTFIIASVIGVLPIAISAFQALK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
VKVISIDLLVTIAVVGAFIIKAYEESAIVTFLFLFGAYLEQRTLNKTRSAIKALTEMAPE
EEEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHH
TALKKMDDGEFLEVGIDDVEVTDILLVKTGAKVPVDGIVLSGSAYINEASITGEAVSVKK
HHHHHCCCCCEEEECCCCEEEEEEEEEECCCCCCCCCEEEECCCEEECCCCCCCHHHHHH
VVDSKVFAGTILDNGTIEIIAQKVGEDTTFGKIIELVEEAQDSKSEAEKFIDRFATWYTP
HHHCHHHEEEEECCCHHEEHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
FVLVLALIVWVITQDVALAITILVLGCPGALVIGVPVSNVAGIGNGARHGVLLKGSEVIG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHCCCCCCCCCCEEEECHHHHC
NFSKVDTIVFDKTGTLTIGHPVLRYEAYYELDKKDIIPYIYSVEKQSDHPLAKAITNHLK
CHHHHCEEEEECCCCEEECCHHHHHHHHHHCCCCCCCCHHEEECCCCCCCHHHHHHHHHC
DAKTLPIDHTDVIKGGGIVASIHERKVFIGNTVLMKDNGIEIKSDIMDEISRLEHEGHSI
CCCCCCCCCHHHCCCCCEEEEEECCEEEECCEEEEECCCCEEHHHHHHHHHHHHCCCCEE
VLTAIDEKLVSLMGIRDEVRPNLKKDLKHIKKMGVKNLIVLSGDNQGSVDIVAKELGLTK
EEEEHHHHHHHHHCCHHHHCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHH
AIGNMLPEDKANYIKELMNEGHIIAFVGDGVNDSPSIALAHIGIAMGGGTDVAIETSDVV
HHHHCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEEEEEECCCCEEEEEECCEE
LMNDNINKLPHALGLAKATKHNMIQNILIAIGVVVVLLVSLFWGEWVNMSIGMLVHEASI
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
LVVILNAMRLLRFKTKIN
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVSFIHKQRMKIMILSFLLIVSGFIAHYVFDQKLIFDVTFIIASVIGVLPIAISAFQALK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
VKVISIDLLVTIAVVGAFIIKAYEESAIVTFLFLFGAYLEQRTLNKTRSAIKALTEMAPE
EEEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHH
TALKKMDDGEFLEVGIDDVEVTDILLVKTGAKVPVDGIVLSGSAYINEASITGEAVSVKK
HHHHHCCCCCEEEECCCCEEEEEEEEEECCCCCCCCCEEEECCCEEECCCCCCCHHHHHH
VVDSKVFAGTILDNGTIEIIAQKVGEDTTFGKIIELVEEAQDSKSEAEKFIDRFATWYTP
HHHCHHHEEEEECCCHHEEHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
FVLVLALIVWVITQDVALAITILVLGCPGALVIGVPVSNVAGIGNGARHGVLLKGSEVIG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHCCCCCCCCCCEEEECHHHHC
NFSKVDTIVFDKTGTLTIGHPVLRYEAYYELDKKDIIPYIYSVEKQSDHPLAKAITNHLK
CHHHHCEEEEECCCCEEECCHHHHHHHHHHCCCCCCCCHHEEECCCCCCCHHHHHHHHHC
DAKTLPIDHTDVIKGGGIVASIHERKVFIGNTVLMKDNGIEIKSDIMDEISRLEHEGHSI
CCCCCCCCCHHHCCCCCEEEEEECCEEEECCEEEEECCCCEEHHHHHHHHHHHHCCCCEE
VLTAIDEKLVSLMGIRDEVRPNLKKDLKHIKKMGVKNLIVLSGDNQGSVDIVAKELGLTK
EEEEHHHHHHHHHCCHHHHCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHH
AIGNMLPEDKANYIKELMNEGHIIAFVGDGVNDSPSIALAHIGIAMGGGTDVAIETSDVV
HHHHCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEEEEEECCCCEEEEEECCEE
LMNDNINKLPHALGLAKATKHNMIQNILIAIGVVVVLLVSLFWGEWVNMSIGMLVHEASI
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
LVVILNAMRLLRFKTKIN
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11302791 [H]