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The map label for this gene is smpB

Identifier: 162447271

GI number: 162447271

Start: 427129

End: 427578

Strand: Direct

Name: smpB

Synonym: ACL_0406

Alternate gene names: 162447271

Gene position: 427129-427578 (Clockwise)

Preceding gene: 162447270

Following gene: 162447272

Centisome position: 28.53

GC content: 30.44

Gene sequence:

>450_bases
ATGAAAATTATTACTCAAAATAAAAAAGCACATTTTGAATATTTTATTGAACAAAAATTCGTGGCTGGTATTAAACTTCA
AGGTAGTGAAGTTAAAAGTATTAGAGCGGGCAAATGTTCAATTAATGAAGCCTATATCACATTTAGAAATGGAGAAGTTT
TTATCTTAAACATGCACATTGCAAAATTTGATGCATCCTCTATTTTTAATCATGAAGAAACGAGAACTAGAAAACTATTA
TTAAACAGAAGCGAAATTGATAAACTTATGGGCGCACAGACTAGAGATGGTATGACCATTATTCCTTTAACTGTAGAACT
TCATGAAGGTTTAATTAAAGTAGTTATTGCACTTGCTAAGGGTAAAAAGTTGTTTGATAAACGTGAAACGATTAAAGAAC
GTGATATCAAACGTGAACAGCAACAATCACTTAAAGGGAAAATGAGATGA

Upstream 100 bases:

>100_bases
GGATATGGGTAAATCTAGAAAGACTAAATCGAAAGCGCCAAAAAGTGGTAAAAAAGGATTTGGACCTAAGAAAAACAGAT
AGTACATAAAGGAATCTTAC

Downstream 100 bases:

>100_bases
ACCAAAAAATAAAAGTGGGATTAATGCTTGGTGGCGGTGGTGCAAAGGGTAGTTACCAACTAGGTGTCATCAAGGCATTA
GAAGAATTAAATCTCTTAAA

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 149; Mature: 149

Protein sequence:

>149_residues
MKIITQNKKAHFEYFIEQKFVAGIKLQGSEVKSIRAGKCSINEAYITFRNGEVFILNMHIAKFDASSIFNHEETRTRKLL
LNRSEIDKLMGAQTRDGMTIIPLTVELHEGLIKVVIALAKGKKLFDKRETIKERDIKREQQQSLKGKMR

Sequences:

>Translated_149_residues
MKIITQNKKAHFEYFIEQKFVAGIKLQGSEVKSIRAGKCSINEAYITFRNGEVFILNMHIAKFDASSIFNHEETRTRKLL
LNRSEIDKLMGAQTRDGMTIIPLTVELHEGLIKVVIALAKGKKLFDKRETIKERDIKREQQQSLKGKMR
>Mature_149_residues
MKIITQNKKAHFEYFIEQKFVAGIKLQGSEVKSIRAGKCSINEAYITFRNGEVFILNMHIAKFDASSIFNHEETRTRKLL
LNRSEIDKLMGAQTRDGMTIIPLTVELHEGLIKVVIALAKGKKLFDKRETIKERDIKREQQQSLKGKMR

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family

Homologues:

Organism=Escherichia coli, GI1788973, Length=147, Percent_Identity=44.8979591836735, Blast_Score=126, Evalue=6e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SSRP_ACHLI (A9NF97)

Other databases:

- EMBL:   CP000896
- RefSeq:   YP_001620403.1
- ProteinModelPortal:   A9NF97
- SMR:   A9NF97
- GeneID:   5804500
- GenomeReviews:   CP000896_GR
- KEGG:   acl:ACL_0406
- HOGENOM:   HBG293422
- OMA:   EKYEAGI
- BioCyc:   ALAI441768:ACL_0406-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00023
- InterPro:   IPR000037
- InterPro:   IPR020081
- Gene3D:   G3DSA:2.40.280.10
- ProDom:   PD004488
- TIGRFAMs:   TIGR00086

Pfam domain/function: PF01668 SmpB; SSF74982 SmpB

EC number: NA

Molecular weight: Translated: 17208; Mature: 17208

Theoretical pI: Translated: 10.60; Mature: 10.60

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIITQNKKAHFEYFIEQKFVAGIKLQGSEVKSIRAGKCSINEAYITFRNGEVFILNMHI
CCEECCCCHHHHHHHHHHHHHCCEEECCCCCCCEECCCCCCCEEEEEEECCEEEEEEEEE
AKFDASSIFNHEETRTRKLLLNRSEIDKLMGAQTRDGMTIIPLTVELHEGLIKVVIALAK
EECCHHHHCCCHHHHHHHHHHCHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHC
GKKLFDKRETIKERDIKREQQQSLKGKMR
CCHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKIITQNKKAHFEYFIEQKFVAGIKLQGSEVKSIRAGKCSINEAYITFRNGEVFILNMHI
CCEECCCCHHHHHHHHHHHHHCCEEECCCCCCCEECCCCCCCEEEEEEECCEEEEEEEEE
AKFDASSIFNHEETRTRKLLLNRSEIDKLMGAQTRDGMTIIPLTVELHEGLIKVVIALAK
EECCHHHHCCCHHHHHHHHHHCHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHC
GKKLFDKRETIKERDIKREQQQSLKGKMR
CCHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA