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The map label for this gene is thrS

Identifier: 162447220

GI number: 162447220

Start: 372064

End: 373992

Strand: Direct

Name: thrS

Synonym: ACL_0354

Alternate gene names: 162447220

Gene position: 372064-373992 (Clockwise)

Preceding gene: 162447219

Following gene: 162447221

Centisome position: 24.85

GC content: 33.13

Gene sequence:

>1929_bases
ATGATTAAAGTAACATTTCCTGATGGCAACATCAAATCTTACAAAAAAGGTATTACAGTATTAGAGGTTGCAGAAGATAT
TTCGATATCTTTAGCAAAAAAAATAGTTGCAGCTAAATTTAATGAGGATTTAGTTGAAATATCACGTAAATTAGAACATG
ATGGTAAATTACAATTACTTACTCCAAAAGAGGAACTATCATTTCATGTATTAAATCACTCGGCCGCACACTTAATGGCA
CAAGCTATTCGTAAACTTTATCCAGATGCATTATTTGGGGTAGGGCCAGCAATTGAAGAAGGCTATTATTATGATGTTGA
TTTTAAAGACAGTGTCTTTACAGATGCAGATTTAGAAGAGGTAGAAAAAATGATGAAAAAATTAAGTGAAGAAAATCACT
TAATCGAACGTATTGAAGTATCCTATGATGAAGCTAGAGACATATTTAAAAATGATCCATATAAATTAGAATTAATTGAA
GTTTATAAAGATGATCAGTTAAGTGTTTATAGACAAGGGGAATTCATCGATTTATGTCGTGGTGGTCACGTACCTTCCAC
AAAATACATCAAACACTTTAAACTCTTAAGTATTGCAGGTGCTTACTGGAGAGGGGATGCTAAAAATAGACAATTAGTAC
GTGTGTATGGTATTGCTTATTTTGAAAAAGCAGAACTTGATAAACATTTAGTGATGCTTGAAGAAAGAAAATTAAGAGAT
CATAGAAAAATCGGTAAAGATTTAGATATCTTTATGACAAGCCAAGAAGTAGGCTCTGGGTTACCATTTTGGTTACCAAA
AGGCGCAACTATTCGCCGCATCATCGAACGTTATATTACTGATAAAGAGTTAGAATTAGGCTATTTACACGTGTATACAC
CAATTATGGCTAACGTAGAGTTTTATAAACAAAGTGGACACTGGGATCACTATCATGAAAACATGTATCCTCCAATGGAT
TTAGGTGATGGAGAAATGTTAGTACTACGTCCAATGAACTGTCCTCACCACATGATGATTTATAAAAAAGATATTCACTC
CTATAGAGAATTGCCAATACGTTTTGCTGAACTAGGTATGATGCACCGTTATGAAAAATCAGGCGCGTTAAGTGGTTTAC
AACGTGTTAGAGAAATGACTTTAAATGATGCACATATCTTTGTAAGACCTGATCAAATTAAAGAAGAATTTATGCGTGTT
GTGCATTTAATTATTGAAGTATATAAAGACTTTAAGATCACTGATTATAGCTTTAGATTAAGCTATAGAGATCCAGAAAA
CAAAGAAAAATACTTTGATGATGATCAAATGTGGCAACGTGCGGAATCAGAACTTAAAAACGTCATGGATGAAATGAAAC
TTCCATACAAAGAAGCCATCGGTGAAGCAGCATTCTATGGACCAAAATTAGATATCCAAGTACGTACTGCAATGGGTATG
GAAGAAACTCTATCCACAGTACAACTAGACTTCTTATTACCGGAACGATTTGACCTAACTTATGTTGGTGAAGATGGTAA
GAATAACCATCGCCCGGTAGTTATTCACCGTGGTGTAGTATCTACAATGGAACGTTTTGTAGCATACTTAATTGAAGAAT
ACAAAGGTAGCTTCCCATTATGGTTAGCACCAGTTCAATTAAAACTGATTCCTGTAAATTTAGACTTACATAAAGATTAT
GTCATGAATCTACATGAACAATTAAAAAAATTAGGATTTAGAGTAGAATCTGACTTTAGAAATGAAAAGTTAGGCTATAA
GATCCGTGAAGCTCAAACATTAAAAATACCATATCAATTAGTGGTAGGTGATAATGAGATGAATACACACTCAATTACCT
ATAGACCATATGGAAGTGAAAAGCAAATTAATATCTCAATTGATGGCTTCATTAAATTGTTAAATGAACGCATGATCAAT
AAAGACTAA

Upstream 100 bases:

>100_bases
CATTAATTGTATGAATTAAGGTGGTACCGCGGATTTTTAAATTCGTCCTTACATTGTTTTATTGTAGGGACTTTTATTTT
TATAAGCAAAGGAGAACGAC

Downstream 100 bases:

>100_bases
GATATATCAACTAATCCTAGTCAACAATAAATCTGCACACACCTGTCATAAATAGGTGTGTGTTTTTATCATAAAGATGG
CCTTAAATCGATTAAATAAT

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS

Number of amino acids: Translated: 642; Mature: 642

Protein sequence:

>642_residues
MIKVTFPDGNIKSYKKGITVLEVAEDISISLAKKIVAAKFNEDLVEISRKLEHDGKLQLLTPKEELSFHVLNHSAAHLMA
QAIRKLYPDALFGVGPAIEEGYYYDVDFKDSVFTDADLEEVEKMMKKLSEENHLIERIEVSYDEARDIFKNDPYKLELIE
VYKDDQLSVYRQGEFIDLCRGGHVPSTKYIKHFKLLSIAGAYWRGDAKNRQLVRVYGIAYFEKAELDKHLVMLEERKLRD
HRKIGKDLDIFMTSQEVGSGLPFWLPKGATIRRIIERYITDKELELGYLHVYTPIMANVEFYKQSGHWDHYHENMYPPMD
LGDGEMLVLRPMNCPHHMMIYKKDIHSYRELPIRFAELGMMHRYEKSGALSGLQRVREMTLNDAHIFVRPDQIKEEFMRV
VHLIIEVYKDFKITDYSFRLSYRDPENKEKYFDDDQMWQRAESELKNVMDEMKLPYKEAIGEAAFYGPKLDIQVRTAMGM
EETLSTVQLDFLLPERFDLTYVGEDGKNNHRPVVIHRGVVSTMERFVAYLIEEYKGSFPLWLAPVQLKLIPVNLDLHKDY
VMNLHEQLKKLGFRVESDFRNEKLGYKIREAQTLKIPYQLVVGDNEMNTHSITYRPYGSEKQINISIDGFIKLLNERMIN
KD

Sequences:

>Translated_642_residues
MIKVTFPDGNIKSYKKGITVLEVAEDISISLAKKIVAAKFNEDLVEISRKLEHDGKLQLLTPKEELSFHVLNHSAAHLMA
QAIRKLYPDALFGVGPAIEEGYYYDVDFKDSVFTDADLEEVEKMMKKLSEENHLIERIEVSYDEARDIFKNDPYKLELIE
VYKDDQLSVYRQGEFIDLCRGGHVPSTKYIKHFKLLSIAGAYWRGDAKNRQLVRVYGIAYFEKAELDKHLVMLEERKLRD
HRKIGKDLDIFMTSQEVGSGLPFWLPKGATIRRIIERYITDKELELGYLHVYTPIMANVEFYKQSGHWDHYHENMYPPMD
LGDGEMLVLRPMNCPHHMMIYKKDIHSYRELPIRFAELGMMHRYEKSGALSGLQRVREMTLNDAHIFVRPDQIKEEFMRV
VHLIIEVYKDFKITDYSFRLSYRDPENKEKYFDDDQMWQRAESELKNVMDEMKLPYKEAIGEAAFYGPKLDIQVRTAMGM
EETLSTVQLDFLLPERFDLTYVGEDGKNNHRPVVIHRGVVSTMERFVAYLIEEYKGSFPLWLAPVQLKLIPVNLDLHKDY
VMNLHEQLKKLGFRVESDFRNEKLGYKIREAQTLKIPYQLVVGDNEMNTHSITYRPYGSEKQINISIDGFIKLLNERMIN
KD
>Mature_642_residues
MIKVTFPDGNIKSYKKGITVLEVAEDISISLAKKIVAAKFNEDLVEISRKLEHDGKLQLLTPKEELSFHVLNHSAAHLMA
QAIRKLYPDALFGVGPAIEEGYYYDVDFKDSVFTDADLEEVEKMMKKLSEENHLIERIEVSYDEARDIFKNDPYKLELIE
VYKDDQLSVYRQGEFIDLCRGGHVPSTKYIKHFKLLSIAGAYWRGDAKNRQLVRVYGIAYFEKAELDKHLVMLEERKLRD
HRKIGKDLDIFMTSQEVGSGLPFWLPKGATIRRIIERYITDKELELGYLHVYTPIMANVEFYKQSGHWDHYHENMYPPMD
LGDGEMLVLRPMNCPHHMMIYKKDIHSYRELPIRFAELGMMHRYEKSGALSGLQRVREMTLNDAHIFVRPDQIKEEFMRV
VHLIIEVYKDFKITDYSFRLSYRDPENKEKYFDDDQMWQRAESELKNVMDEMKLPYKEAIGEAAFYGPKLDIQVRTAMGM
EETLSTVQLDFLLPERFDLTYVGEDGKNNHRPVVIHRGVVSTMERFVAYLIEEYKGSFPLWLAPVQLKLIPVNLDLHKDY
VMNLHEQLKKLGFRVESDFRNEKLGYKIREAQTLKIPYQLVVGDNEMNTHSITYRPYGSEKQINISIDGFIKLLNERMIN
KD

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI38570111, Length=650, Percent_Identity=36.4615384615385, Blast_Score=410, Evalue=1e-114,
Organism=Homo sapiens, GI38202255, Length=619, Percent_Identity=37.3182552504039, Blast_Score=409, Evalue=1e-114,
Organism=Homo sapiens, GI20070344, Length=664, Percent_Identity=33.1325301204819, Blast_Score=359, Evalue=6e-99,
Organism=Escherichia coli, GI1788013, Length=619, Percent_Identity=40.2261712439418, Blast_Score=503, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI71984192, Length=633, Percent_Identity=34.7551342812006, Blast_Score=400, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI71984184, Length=633, Percent_Identity=34.7551342812006, Blast_Score=399, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6322112, Length=633, Percent_Identity=35.0710900473934, Blast_Score=387, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6322654, Length=400, Percent_Identity=36, Blast_Score=242, Evalue=2e-64,
Organism=Drosophila melanogaster, GI24583842, Length=648, Percent_Identity=35.9567901234568, Blast_Score=414, Evalue=1e-116,
Organism=Drosophila melanogaster, GI28574141, Length=648, Percent_Identity=35.9567901234568, Blast_Score=414, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24583839, Length=648, Percent_Identity=35.9567901234568, Blast_Score=414, Evalue=1e-115,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYT_ACHLI (A9NF46)

Other databases:

- EMBL:   CP000896
- RefSeq:   YP_001620352.1
- ProteinModelPortal:   A9NF46
- SMR:   A9NF46
- GeneID:   5804340
- GenomeReviews:   CP000896_GR
- KEGG:   acl:ACL_0354
- HOGENOM:   HBG352811
- OMA:   NDETMST
- BioCyc:   ALAI441768:ACL_0354-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00184_B
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.10.20.30
- PRINTS:   PR01047
- SMART:   SM00863
- TIGRFAMs:   TIGR00418

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD; SSF52954 Anticodon_bd; SSF81271 TGS-like; SSF55186 Thr/Ala-tRNA-synth_IIc_edit

EC number: =6.1.1.3

Molecular weight: Translated: 75293; Mature: 75293

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKVTFPDGNIKSYKKGITVLEVAEDISISLAKKIVAAKFNEDLVEISRKLEHDGKLQLL
CEEEECCCCCHHHHHCCCEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEE
TPKEELSFHVLNHSAAHLMAQAIRKLYPDALFGVGPAIEEGYYYDVDFKDSVFTDADLEE
CCHHHCEEEEECCHHHHHHHHHHHHHCCHHHHCCCCHHHCCEEEEECCCCCCCCCCCHHH
VEKMMKKLSEENHLIERIEVSYDEARDIFKNDPYKLELIEVYKDDQLSVYRQGEFIDLCR
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEEEEECCCCEEEEECCCEEEECC
GGHVPSTKYIKHFKLLSIAGAYWRGDAKNRQLVRVYGIAYFEKAELDKHLVMLEERKLRD
CCCCCCHHHHHHHHHHHHHCHHHCCCCCCCEEEEEECCHHHHHHHCCHHHHHHHHHHHHH
HRKIGKDLDIFMTSQEVGSGLPFWLPKGATIRRIIERYITDKELELGYLHVYTPIMANVE
HHHCCCCCEEEEECHHHCCCCCEECCCCHHHHHHHHHHCCCCCCEEEEEEEEHHHHHCHH
FYKQSGHWDHYHENMYPPMDLGDGEMLVLRPMNCPHHMMIYKKDIHSYRELPIRFAELGM
HHHCCCCCCCHHCCCCCCCCCCCCCEEEEECCCCCCHHEEEHHHHHHHHHCCHHHHHHHH
MHRYEKSGALSGLQRVREMTLNDAHIFVRPDQIKEEFMRVVHLIIEVYKDFKITDYSFRL
HHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCEEEEEEEEE
SYRDPENKEKYFDDDQMWQRAESELKNVMDEMKLPYKEAIGEAAFYGPKLDIQVRTAMGM
ECCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHCCCCEEEEEHHHCCH
EETLSTVQLDFLLPERFDLTYVGEDGKNNHRPVVIHRGVVSTMERFVAYLIEEYKGSFPL
HHHHHHEEEEEECCCCCCEEEECCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCE
WLAPVQLKLIPVNLDLHKDYVMNLHEQLKKLGFRVESDFRNEKLGYKIREAQTLKIPYQL
EEECEEEEEEEEEECCCHHHHHHHHHHHHHHCCEECCCCCCCCCCEEEECCEEEECCEEE
VVGDNEMNTHSITYRPYGSEKQINISIDGFIKLLNERMINKD
EECCCCCCCCEEEECCCCCCCEEEEEHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIKVTFPDGNIKSYKKGITVLEVAEDISISLAKKIVAAKFNEDLVEISRKLEHDGKLQLL
CEEEECCCCCHHHHHCCCEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEE
TPKEELSFHVLNHSAAHLMAQAIRKLYPDALFGVGPAIEEGYYYDVDFKDSVFTDADLEE
CCHHHCEEEEECCHHHHHHHHHHHHHCCHHHHCCCCHHHCCEEEEECCCCCCCCCCCHHH
VEKMMKKLSEENHLIERIEVSYDEARDIFKNDPYKLELIEVYKDDQLSVYRQGEFIDLCR
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEEEEECCCCEEEEECCCEEEECC
GGHVPSTKYIKHFKLLSIAGAYWRGDAKNRQLVRVYGIAYFEKAELDKHLVMLEERKLRD
CCCCCCHHHHHHHHHHHHHCHHHCCCCCCCEEEEEECCHHHHHHHCCHHHHHHHHHHHHH
HRKIGKDLDIFMTSQEVGSGLPFWLPKGATIRRIIERYITDKELELGYLHVYTPIMANVE
HHHCCCCCEEEEECHHHCCCCCEECCCCHHHHHHHHHHCCCCCCEEEEEEEEHHHHHCHH
FYKQSGHWDHYHENMYPPMDLGDGEMLVLRPMNCPHHMMIYKKDIHSYRELPIRFAELGM
HHHCCCCCCCHHCCCCCCCCCCCCCEEEEECCCCCCHHEEEHHHHHHHHHCCHHHHHHHH
MHRYEKSGALSGLQRVREMTLNDAHIFVRPDQIKEEFMRVVHLIIEVYKDFKITDYSFRL
HHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCEEEEEEEEE
SYRDPENKEKYFDDDQMWQRAESELKNVMDEMKLPYKEAIGEAAFYGPKLDIQVRTAMGM
ECCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHCCCCEEEEEHHHCCH
EETLSTVQLDFLLPERFDLTYVGEDGKNNHRPVVIHRGVVSTMERFVAYLIEEYKGSFPL
HHHHHHEEEEEECCCCCCEEEECCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCE
WLAPVQLKLIPVNLDLHKDYVMNLHEQLKKLGFRVESDFRNEKLGYKIREAQTLKIPYQL
EEECEEEEEEEEEECCCHHHHHHHHHHHHHHCCEECCCCCCCCCCEEEECCEEEECCEEE
VVGDNEMNTHSITYRPYGSEKQINISIDGFIKLLNERMINKD
EECCCCCCCCEEEECCCCCCCEEEEEHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA