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The map label for this gene is licH [H]

Identifier: 162447133

GI number: 162447133

Start: 263500

End: 264798

Strand: Direct

Name: licH [H]

Synonym: ACL_0264

Alternate gene names: 162447133

Gene position: 263500-264798 (Clockwise)

Preceding gene: 162447132

Following gene: 162447134

Centisome position: 17.6

GC content: 30.95

Gene sequence:

>1299_bases
ATGAATAAAAAATTAAAATTAGTAATTATTGGTGCAGGATCAAGTTACACACCAGAATTATTTAAACACATCATATTAAA
CTATGAAGATTTAAAAGTAACTGAGATTGCACTAGTTGATTTAGAGGATAATCAAAACAGATTAAACATTATAAATGATT
TTGCACACAGAATGTTTAAGAAGCATAATATTTCAATGGCTATTTCTCAATCAATAAATAGAAGAGAAGCTTTAGTTGAT
GCAGACTTTGTACTTATTCAAATTAGAGTGGGACGTATGGAAGCAAGGTACTTCGATGAAACTGTTCCTGCACAATTTGA
AATGTTAGGACATGAAGCAATTGGTATCGGAGGGATGTTTAATGCACTAAGAACTATCCCAGTAATCTATAAAATTATTG
AAGATATTAAAGAACTTGCACCTAAGGCATGGGTTATTAATATTAGTAATCCTACTGGAATCATTAGTGAAGCAGTATTT
AGATTTGCAGAGTTTGAACGCTACATGGGGTTATCAAGTAGTCCAAATCAAGCAACAAAAAGTATCATTGAAAAATTAGG
TGCAAAACCTTCTGAAGTTGTGCCTTATTTTGCGGGATTAAGTGAATTATCATTTATCTCAAAAATATATCATAAGAGTA
AAGATAAACTGCCTAAACTATTAGAAGAAGACTTTTGTCCATCTAAAAGATTTATGGCACCTGAAAACCTAAAACAGCTT
AAATTATACCCGCATCCAAACTTAAAGGTCTATTATCAATATGATATTGCAGTACCTGAGTTTTTAGAACATATTAGAAA
TAATCAGATACGTACAAATGAAGTTATTCAAATAGATGAAGAATTATTTAAACAATATAGTGACCCTTCAGTAGGTGAAG
TACCTAAAGATATTGAGAAAAGATTAGGTCATGATTATGCAGAAACAGCGATCCAAATCATTGATTCTATGGTGAATAAT
AAAAAGAACTATCACGTCATTAATACAGTCAATCGTGGCCATATTGTAGGAATTCCAGATGAAACATCTATAGAAATCAC
TTCACGTATTACAAATAAGGGTCCGATTCCGGTCCATATTGGTGAACTACCACTCCAAATACGTGGTATCGTTCAACATC
TAAAAGCTTATGAAGAATTATTATGTGATGCGATATATGAAAAGAATTTGAATAAAGCATTACTTGCATATCAGGTACAT
CCTTTATCTAAATCATTTATTACAACTAAACATGCATTTGATGCATTATATGAAAAACATAAAAACTTACTAACATACTA
TGGAGCGTACAACAAATGA

Upstream 100 bases:

>100_bases
AATGTATCAAGAACCGTCCAATCTACCTACAGCAACTGGTGCAAAAAAAGCTGTCGTACTTGGTAATGTTTCTTACTACA
GATAAAAAAAGGGGCTAAAC

Downstream 100 bases:

>100_bases
AACTAAACTATTTTGGAACTGATAAACCACTGAATTACTTTAATACAGATAAACAAGTCGTGATACCTGAAAAATTTAAC
TTAAAACCATTTAGAGCATT

Product: glycosyl hydrolase family 4 protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MNKKLKLVIIGAGSSYTPELFKHIILNYEDLKVTEIALVDLEDNQNRLNIINDFAHRMFKKHNISMAISQSINRREALVD
ADFVLIQIRVGRMEARYFDETVPAQFEMLGHEAIGIGGMFNALRTIPVIYKIIEDIKELAPKAWVINISNPTGIISEAVF
RFAEFERYMGLSSSPNQATKSIIEKLGAKPSEVVPYFAGLSELSFISKIYHKSKDKLPKLLEEDFCPSKRFMAPENLKQL
KLYPHPNLKVYYQYDIAVPEFLEHIRNNQIRTNEVIQIDEELFKQYSDPSVGEVPKDIEKRLGHDYAETAIQIIDSMVNN
KKNYHVINTVNRGHIVGIPDETSIEITSRITNKGPIPVHIGELPLQIRGIVQHLKAYEELLCDAIYEKNLNKALLAYQVH
PLSKSFITTKHAFDALYEKHKNLLTYYGAYNK

Sequences:

>Translated_432_residues
MNKKLKLVIIGAGSSYTPELFKHIILNYEDLKVTEIALVDLEDNQNRLNIINDFAHRMFKKHNISMAISQSINRREALVD
ADFVLIQIRVGRMEARYFDETVPAQFEMLGHEAIGIGGMFNALRTIPVIYKIIEDIKELAPKAWVINISNPTGIISEAVF
RFAEFERYMGLSSSPNQATKSIIEKLGAKPSEVVPYFAGLSELSFISKIYHKSKDKLPKLLEEDFCPSKRFMAPENLKQL
KLYPHPNLKVYYQYDIAVPEFLEHIRNNQIRTNEVIQIDEELFKQYSDPSVGEVPKDIEKRLGHDYAETAIQIIDSMVNN
KKNYHVINTVNRGHIVGIPDETSIEITSRITNKGPIPVHIGELPLQIRGIVQHLKAYEELLCDAIYEKNLNKALLAYQVH
PLSKSFITTKHAFDALYEKHKNLLTYYGAYNK
>Mature_432_residues
MNKKLKLVIIGAGSSYTPELFKHIILNYEDLKVTEIALVDLEDNQNRLNIINDFAHRMFKKHNISMAISQSINRREALVD
ADFVLIQIRVGRMEARYFDETVPAQFEMLGHEAIGIGGMFNALRTIPVIYKIIEDIKELAPKAWVINISNPTGIISEAVF
RFAEFERYMGLSSSPNQATKSIIEKLGAKPSEVVPYFAGLSELSFISKIYHKSKDKLPKLLEEDFCPSKRFMAPENLKQL
KLYPHPNLKVYYQYDIAVPEFLEHIRNNQIRTNEVIQIDEELFKQYSDPSVGEVPKDIEKRLGHDYAETAIQIIDSMVNN
KKNYHVINTVNRGHIVGIPDETSIEITSRITNKGPIPVHIGELPLQIRGIVQHLKAYEELLCDAIYEKNLNKALLAYQVH
PLSKSFITTKHAFDALYEKHKNLLTYYGAYNK

Specific function: Hydrolyzes phospho-beta-glucosides (Probable) [H]

COG id: COG1486

COG function: function code G; Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 4 family [H]

Homologues:

Organism=Escherichia coli, GI1788029, Length=418, Percent_Identity=34.2105263157895, Blast_Score=254, Evalue=8e-69,
Organism=Escherichia coli, GI1790560, Length=456, Percent_Identity=22.5877192982456, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019802
- InterPro:   IPR001088
- InterPro:   IPR022616
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02056 Glyco_hydro_4; PF11975 Glyco_hydro_4C [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 49519; Mature: 49519

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKLKLVIIGAGSSYTPELFKHIILNYEDLKVTEIALVDLEDNQNRLNIINDFAHRMFK
CCCEEEEEEEECCCCCCHHHHHHHHCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHH
KHNISMAISQSINRREALVDADFVLIQIRVGRMEARYFDETVPAQFEMLGHEAIGIGGMF
HCCCHHHHHHHCHHHHHHHCCCEEEEEEEECHHHHHHHHCCCCHHHHHHCCHHCCCCHHH
NALRTIPVIYKIIEDIKELAPKAWVINISNPTGIISEAVFRFAEFERYMGLSSSPNQATK
HHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHH
SIIEKLGAKPSEVVPYFAGLSELSFISKIYHKSKDKLPKLLEEDFCPSKRFMAPENLKQL
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCHHHE
KLYPHPNLKVYYQYDIAVPEFLEHIRNNQIRTNEVIQIDEELFKQYSDPSVGEVPKDIEK
EECCCCCEEEEEEECCCHHHHHHHHCCCCCCCCHHEEHHHHHHHHCCCCCCCCCCHHHHH
RLGHDYAETAIQIIDSMVNNKKNYHVINTVNRGHIVGIPDETSIEITSRITNKGPIPVHI
HHCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEECCCCCCEEEEEHHCCCCCCEEEE
GELPLQIRGIVQHLKAYEELLCDAIYEKNLNKALLAYQVHPLSKSFITTKHAFDALYEKH
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHH
KNLLTYYGAYNK
HHHEEECCCCCC
>Mature Secondary Structure
MNKKLKLVIIGAGSSYTPELFKHIILNYEDLKVTEIALVDLEDNQNRLNIINDFAHRMFK
CCCEEEEEEEECCCCCCHHHHHHHHCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHH
KHNISMAISQSINRREALVDADFVLIQIRVGRMEARYFDETVPAQFEMLGHEAIGIGGMF
HCCCHHHHHHHCHHHHHHHCCCEEEEEEEECHHHHHHHHCCCCHHHHHHCCHHCCCCHHH
NALRTIPVIYKIIEDIKELAPKAWVINISNPTGIISEAVFRFAEFERYMGLSSSPNQATK
HHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHH
SIIEKLGAKPSEVVPYFAGLSELSFISKIYHKSKDKLPKLLEEDFCPSKRFMAPENLKQL
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCHHHE
KLYPHPNLKVYYQYDIAVPEFLEHIRNNQIRTNEVIQIDEELFKQYSDPSVGEVPKDIEK
EECCCCCEEEEEEECCCHHHHHHHHCCCCCCCCHHEEHHHHHHHHCCCCCCCCCCHHHHH
RLGHDYAETAIQIIDSMVNNKKNYHVINTVNRGHIVGIPDETSIEITSRITNKGPIPVHI
HHCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEECCCCCCEEEEEHHCCCCCCEEEE
GELPLQIRGIVQHLKAYEELLCDAIYEKNLNKALLAYQVHPLSKSFITTKHAFDALYEKH
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHH
KNLLTYYGAYNK
HHHEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8990303; 9384377; 8969509 [H]