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The map label for this gene is pheS

Identifier: 162447119

GI number: 162447119

Start: 248035

End: 249048

Strand: Direct

Name: pheS

Synonym: ACL_0249

Alternate gene names: 162447119

Gene position: 248035-249048 (Clockwise)

Preceding gene: 162447118

Following gene: 162447120

Centisome position: 16.57

GC content: 33.33

Gene sequence:

>1014_bases
ATGAAGGAAAAAATAGATTCGATTGTTAAATCATTAAATGATAAAATCCATCCGGATTTAACATTAAATGATTTAGAAGC
ATTAAGAGTTGAGTATCTAGGAAAAAATGGTATTTTAACATCTCAAATGTCTCTAATTAGAACTTTACCAAATGAAGAAA
AACCTAAGTTTGGTCAATGGATCAATGATGCAAAGGTTGCAATAGAACAAGTAATTAGTGATCAAAAAAACATATTACTT
ACAATAGCAATGAATGAACAATTAAAAAAAGAAACACTTGATGTTACAATGCCAGGGTTAAACTTTAAAAAAGGTAGCTT
ACACCCATTAACATTAGTGACTCAAGATTTAGAAGATTACTTTATTGGTTTAGGCTATAAAGTTGCTGAAGGTCCAGAAC
TAGAGTTAGATAAATATAACTTTGAAATGATGAATCTAGCAAGTGATCACCCTGCTAGAGAAATGCATGATTCTTTTTAT
GTAACTCAAAATACATTACTAAGAACACATACATCTCCAATTCAAGCACGCTATATGGAAAAATCAAAGGGGCAACCAAT
CGCAATTATTGCTCCAGGTAAAACCTATAGAAGAGATCCAGATGATCGTACGCACTCACACCAATTTATGCAATGTGAAG
GTTTAGTCATTGATAAGGATGTGACATTTGCACACCTTAAAGAAACGCTACTTGGTATGGCACGTCATATCTTTGGTGAA
GAAAGAACGATTCGTTTAAGACCATCTTACTTCCCATTTACTGAACCATCTGTTGAAGTCGATGTATCTTATGTGGACAG
TGATGGTAAAACAGAGTGGATCGAAGTTTTAGGTGCTGGTATGGTACACCCAAATGTGTTAACTATGGGTGGATATGATC
CTAAAGTATACCGTGGGTTTGCTTTTGGGATTGGACTTGAACGTATTGCCATCTTAAAATATAAAATCGATGACATTCGT
CAATTTTATACTAATGATCTACGCTTCTTAAATCAATTTAAAGGAGTCTTATAA

Upstream 100 bases:

>100_bases
TTTTCTTGGAACTAAGGTGGTACCGCGATTGATCGTCCTTAGCGTATATTTATACGTCTAAGGACTTTTATTTTAAATTT
GATAAGGAGAATTATAGAAA

Downstream 100 bases:

>100_bases
TGTTTATATTAGATAATATATTAAAACAATTTATTGAAGTACCAAATGATTTATATGAAAAGACCAATCAACAAATCATC
GAAGTCGATGATTATTTAGC

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MKEKIDSIVKSLNDKIHPDLTLNDLEALRVEYLGKNGILTSQMSLIRTLPNEEKPKFGQWINDAKVAIEQVISDQKNILL
TIAMNEQLKKETLDVTMPGLNFKKGSLHPLTLVTQDLEDYFIGLGYKVAEGPELELDKYNFEMMNLASDHPAREMHDSFY
VTQNTLLRTHTSPIQARYMEKSKGQPIAIIAPGKTYRRDPDDRTHSHQFMQCEGLVIDKDVTFAHLKETLLGMARHIFGE
ERTIRLRPSYFPFTEPSVEVDVSYVDSDGKTEWIEVLGAGMVHPNVLTMGGYDPKVYRGFAFGIGLERIAILKYKIDDIR
QFYTNDLRFLNQFKGVL

Sequences:

>Translated_337_residues
MKEKIDSIVKSLNDKIHPDLTLNDLEALRVEYLGKNGILTSQMSLIRTLPNEEKPKFGQWINDAKVAIEQVISDQKNILL
TIAMNEQLKKETLDVTMPGLNFKKGSLHPLTLVTQDLEDYFIGLGYKVAEGPELELDKYNFEMMNLASDHPAREMHDSFY
VTQNTLLRTHTSPIQARYMEKSKGQPIAIIAPGKTYRRDPDDRTHSHQFMQCEGLVIDKDVTFAHLKETLLGMARHIFGE
ERTIRLRPSYFPFTEPSVEVDVSYVDSDGKTEWIEVLGAGMVHPNVLTMGGYDPKVYRGFAFGIGLERIAILKYKIDDIR
QFYTNDLRFLNQFKGVL
>Mature_337_residues
MKEKIDSIVKSLNDKIHPDLTLNDLEALRVEYLGKNGILTSQMSLIRTLPNEEKPKFGQWINDAKVAIEQVISDQKNILL
TIAMNEQLKKETLDVTMPGLNFKKGSLHPLTLVTQDLEDYFIGLGYKVAEGPELELDKYNFEMMNLASDHPAREMHDSFY
VTQNTLLRTHTSPIQARYMEKSKGQPIAIIAPGKTYRRDPDDRTHSHQFMQCEGLVIDKDVTFAHLKETLLGMARHIFGE
ERTIRLRPSYFPFTEPSVEVDVSYVDSDGKTEWIEVLGAGMVHPNVLTMGGYDPKVYRGFAFGIGLERIAILKYKIDDIR
QFYTNDLRFLNQFKGVL

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:
GO:0000049: Phenylalanyl-tRNA synthetase alpha chain
GO:0000166: Phenylalanyl-tRNA synthetase alpha chain
GO:0004812: Phenylalanyl-tRNA synthetase alpha chain
GO:0004826: Phenylalanyl-tRNA synthetase alpha chain
GO:0005524: Phenylalanyl-tRNA synthetase alpha chain
GO:0005737: Phenylalanyl-tRNA synthetase alpha chain
GO:0006412: Phenylalanyl-tRNA synthetase alpha chain
GO:0006418: Phenylalanyl-tRNA synthetase alpha chain
GO:0006432: Phenylalanyl-tRNA synthetase alpha chain
GO:0016874: Phenylalanyl-tRNA synthetase alpha chain
GO:0043039: Phenylalanyl-tRNA synthetase alpha chain
GO:0046872: Phenylalanyl-tRNA synthetase alpha chain

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5729820, Length=283, Percent_Identity=30.0353356890459, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI4758340, Length=266, Percent_Identity=28.9473684210526, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI1788007, Length=327, Percent_Identity=49.2354740061162, Blast_Score=317, Evalue=6e-88,
Organism=Caenorhabditis elegans, GI32566635, Length=237, Percent_Identity=33.7552742616034, Blast_Score=117, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI17508957, Length=282, Percent_Identity=27.6595744680851, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI32563657, Length=282, Percent_Identity=27.6595744680851, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6321087, Length=282, Percent_Identity=25.886524822695, Blast_Score=111, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6325304, Length=137, Percent_Identity=33.5766423357664, Blast_Score=86, Evalue=7e-18,
Organism=Drosophila melanogaster, GI17137424, Length=293, Percent_Identity=28.6689419795222, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI18858079, Length=289, Percent_Identity=28.0276816608997, Blast_Score=104, Evalue=9e-23,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_ACHLI (A9NEU5)

Other databases:

- EMBL:   CP000896
- RefSeq:   YP_001620251.1
- ProteinModelPortal:   A9NEU5
- SMR:   A9NEU5
- GeneID:   5804105
- GenomeReviews:   CP000896_GR
- KEGG:   acl:ACL_0249
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- BioCyc:   ALAI441768:ACL_0249-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 38574; Mature: 38574

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEKIDSIVKSLNDKIHPDLTLNDLEALRVEYLGKNGILTSQMSLIRTLPNEEKPKFGQW
CHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCHHHH
INDAKVAIEQVISDQKNILLTIAMNEQLKKETLDVTMPGLNFKKGSLHPLTLVTQDLEDY
HHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHEEEECCCCCCCCCCCCHHHHHHHHHHH
FIGLGYKVAEGPELELDKYNFEMMNLASDHPAREMHDSFYVTQNTLLRTHTSPIQARYME
HHHCCEEECCCCCCEEECCCEEEEECCCCCCHHHHHCCEEEECCCEEEECCCHHHHHHHH
KSKGQPIAIIAPGKTYRRDPDDRTHSHQFMQCEGLVIDKDVTFAHLKETLLGMARHIFGE
HCCCCEEEEECCCCCCCCCCCCCCCCCCEEEECCEEEECCCHHHHHHHHHHHHHHHHCCC
ERTIRLRPSYFPFTEPSVEVDVSYVDSDGKTEWIEVLGAGMVHPNVLTMGGYDPKVYRGF
CCEEEECCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHCCCCCCCEEEECCCCCHHHHHH
AFGIGLERIAILKYKIDDIRQFYTNDLRFLNQFKGVL
HECCCHHHEEEEEECHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKEKIDSIVKSLNDKIHPDLTLNDLEALRVEYLGKNGILTSQMSLIRTLPNEEKPKFGQW
CHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCHHHH
INDAKVAIEQVISDQKNILLTIAMNEQLKKETLDVTMPGLNFKKGSLHPLTLVTQDLEDY
HHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHEEEECCCCCCCCCCCCHHHHHHHHHHH
FIGLGYKVAEGPELELDKYNFEMMNLASDHPAREMHDSFYVTQNTLLRTHTSPIQARYME
HHHCCEEECCCCCCEEECCCEEEEECCCCCCHHHHHCCEEEECCCEEEECCCHHHHHHHH
KSKGQPIAIIAPGKTYRRDPDDRTHSHQFMQCEGLVIDKDVTFAHLKETLLGMARHIFGE
HCCCCEEEEECCCCCCCCCCCCCCCCCCEEEECCEEEECCCHHHHHHHHHHHHHHHHCCC
ERTIRLRPSYFPFTEPSVEVDVSYVDSDGKTEWIEVLGAGMVHPNVLTMGGYDPKVYRGF
CCEEEECCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHCCCCCCCEEEECCCCCHHHHHH
AFGIGLERIAILKYKIDDIRQFYTNDLRFLNQFKGVL
HECCCHHHEEEEEECHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA