| Definition | Acholeplasma laidlawii PG-8A chromosome, complete genome. |
|---|---|
| Accession | NC_010163 |
| Length | 1,496,992 |
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The map label for this gene is adhE [H]
Identifier: 162447047
GI number: 162447047
Start: 165156
End: 167744
Strand: Direct
Name: adhE [H]
Synonym: ACL_0177
Alternate gene names: 162447047
Gene position: 165156-167744 (Clockwise)
Preceding gene: 162447046
Following gene: 162447048
Centisome position: 11.03
GC content: 35.15
Gene sequence:
>2589_bases ATGGAAAAAGACACACTTATCTTAAACGAGGTAGATCTTTTAGTCACGTCTGCTTTAGAAGCACTTGACGAATACATGGA GTTTACCCAAGAGAAGATTGATGAGATTGTAGCCAAAGTTTCTGTTGAAGCAATTGATGCACATGGCCGTTTAGCCAAGC TTGCTGTAGAGGAAACTAAACGAGGGGTATTTGAAGATAAAGCAACCAAGAATTTATTTTCAGTAGAATATGTCATCAAT CATATGAGACACCTAAAAACTGTAGGGATTATCTCGGAAAATACAGTAACGGGTATTACAGAGGTAGCTGATCCTGTTGG GGTAATTTGCGCTATTACACCAGTAACGAATCCAACATCGACGACAATATTTAAGTCATTGATTGCACTTAAAACTAGAA ATCCAATCGTCTTTGCGTTTCATCCAAATGCGCAAAATTCATCAAAGGAAGCGGCTAGAATTATTAAAGAAGCAGCGATT AAATCAGGTGCTCCAAAAAATTGTATTTCATGGATTGAACATCCAAGTTTAGATAATACAAATAAACTTATGAACCACCC GGGGATTGCAACTATTTTAGCAACTGGTGGTAATGCAATGGTTAAGGCAGCTTACTCATGTGGTAAACCAGCTCTTGGTG TCGGTGCAGGTAACGTACCAGCATACATTGAAAAAACTGCAAACATTAAAACCGCAATCAATGATATTGTTATGTCTAAG TCATTTGATAATGGTATGGTATGTGCATCTGAACAAGCAGCTATTATTGATAAAGAAATCTATAAAGAAGCTTTGGCGGA ATTTAAAAAGTTACATACTTTTTTATGTAACAAAGAACAAAAACAAATGCTGGAAACATTTATGTTCCCAGATGGTGTCA AGCTTAATCCTTATGTTGTAGGTAAGAGTGCACATCACATTGCAGAACTTGCAGGTTTCGAAGTTCCTTTAAATACCAAC ATTTTACTAGTAGAATGTAAAAGTGTTGGTAAAGAAGAACCACTTACAAGAGAAAAACTCTCGCCAGTATTAGCAATTCT TAAATCAAATGATGAAGTACATGGTGTAGCACTTGCAAAAAGTATGGTAGAACTTGATGGTTTAGGACACTCTGCAGTGA TTCACACACAAAATGATGAACTTAAAAAAACCTTTGGTTTAGCACTTAAAGCGATACGTATTATAGTGAATTCACCAGCG ACTTTTGGTGGCATAGGTAATGTATATAACAGGTTTATACCATCACTTACCTTAGGGTGTGGTTCTTATGGTAAAAACTC TGTTTCTGATAATGTCAGTGCCATCCACTTACTTAATATCAAACGTATCGGTGAAAGGAAAAATAATATGCAATGGTTTA AGGTTCCATCTAAAATTTACTTTGAAAAAGATTCTATTGAATACTTAAGACAAATGAGAGATGTTAACAAGGTTGTCATC ATCACAGATAGAGTGATGGTTGACTTAGGTTATGTTAAAAAAGTAACTGATCAACTAAGTTTAAGACGTAATCATGTTGT ATATCAACTATTTACCGATGTTGAAGCTGATCCATCCATTGAAACAGTTCATAAAGGTTATGAACTGATGAAATCCTTCC AACCAGATACGATTATAGCTATTGGTGGTGGTTCACCAATGGATGCTGCAAAAGCTATGTGGTTATTCTATGAAAACCCT GAAGTAGATTTTAACGACTTAAAACAAAAATTCATGGATATTAGAAAACGTGCATTTAGATATCCTGATTTAGGTAGAAA ATCTAAATTAGTATGTATTCCAACAACCTCAGGTACTGGTAGTGAAGTGACTCCGTTTGCAGTTATCACTGATAATAAAG AGTCTAAAAAATATCCATTAGCAGATTATTCATTAACACCAACTGTAGCTATTATTGACCCTGCACTTACACTTACTCAA CCAAAATCTGTTACAGCAGATACTGGAATGGACGTGTTAACACATGCAGTAGAAGCTTACGTTTCAGTACTTGCTAATGA CTATACAGACTCACTTGCAATTCAAGCAGTTAAAATGGTATTTAAATACTTAAAACGTGCATTTGATAATGGATTGGACT TTGAAGCAAGAGAAAAGATGCATAATGCATCTACCATGGCAGGTATGGCATTTGGTAATGCATTCTTGGGTATATCTCAC TCTATGGCTCATAAAGTAGGTGGAAGATATCATATACCTCATGGTAGAATTAATGCAATCTTATTACCACATGTCGTTTT ATATAATGGTACAGTTCCAGCTAAGTTATCTACTTGGCCAAAATACACATATTATGTAGCAGATGAAAAATACATGGAAC TAGCTAGAGCAATCGGATTAGATCCTAAATCTAAAGAAGAAGGCGTTAAGATGTTTGCTAAGGCATGTCATGATTTAGCA GTATCATTAGGTATTAATATGAGTTTTGCTAGTCAAAAAGTAGAAGAAAAAGATTTCTTAGAACACAGTGAAACTTTAGC TTATCTAGCTTATGAGGATCAATGTTCAACAGCTAACCCTAGACTACCTTTAGTAGCGGATATGAAGGTGATCCTAGAAA ATGCATATTACGGCAAACTAGATATATAG
Upstream 100 bases:
>100_bases TTTATGAAAACGTATTCATTATAGTTTCATTTGAATTAATTTTAAAATCATATAAAATAGGGCTATAAAACCAATTCATA TAACACAAGGAGAATTTAAT
Downstream 100 bases:
>100_bases TATATATTCAAATAATTAAATCAAAGACTTTCAAATAACTTATAGGTTTTAGTAAATCTTGTAAGACAAAAGTTATTTGA AAGTCTTTTTTTACTATTTA
Product: bifunctional aldehyde/alcohol dehydrogenase
Products: NA
Alternate protein names: Alcohol dehydrogenase; ADH; Acetaldehyde dehydrogenase [acetylating]; ACDH; Pyruvate-formate-lyase deactivase; PFL deactivase [H]
Number of amino acids: Translated: 862; Mature: 862
Protein sequence:
>862_residues MEKDTLILNEVDLLVTSALEALDEYMEFTQEKIDEIVAKVSVEAIDAHGRLAKLAVEETKRGVFEDKATKNLFSVEYVIN HMRHLKTVGIISENTVTGITEVADPVGVICAITPVTNPTSTTIFKSLIALKTRNPIVFAFHPNAQNSSKEAARIIKEAAI KSGAPKNCISWIEHPSLDNTNKLMNHPGIATILATGGNAMVKAAYSCGKPALGVGAGNVPAYIEKTANIKTAINDIVMSK SFDNGMVCASEQAAIIDKEIYKEALAEFKKLHTFLCNKEQKQMLETFMFPDGVKLNPYVVGKSAHHIAELAGFEVPLNTN ILLVECKSVGKEEPLTREKLSPVLAILKSNDEVHGVALAKSMVELDGLGHSAVIHTQNDELKKTFGLALKAIRIIVNSPA TFGGIGNVYNRFIPSLTLGCGSYGKNSVSDNVSAIHLLNIKRIGERKNNMQWFKVPSKIYFEKDSIEYLRQMRDVNKVVI ITDRVMVDLGYVKKVTDQLSLRRNHVVYQLFTDVEADPSIETVHKGYELMKSFQPDTIIAIGGGSPMDAAKAMWLFYENP EVDFNDLKQKFMDIRKRAFRYPDLGRKSKLVCIPTTSGTGSEVTPFAVITDNKESKKYPLADYSLTPTVAIIDPALTLTQ PKSVTADTGMDVLTHAVEAYVSVLANDYTDSLAIQAVKMVFKYLKRAFDNGLDFEAREKMHNASTMAGMAFGNAFLGISH SMAHKVGGRYHIPHGRINAILLPHVVLYNGTVPAKLSTWPKYTYYVADEKYMELARAIGLDPKSKEEGVKMFAKACHDLA VSLGINMSFASQKVEEKDFLEHSETLAYLAYEDQCSTANPRLPLVADMKVILENAYYGKLDI
Sequences:
>Translated_862_residues MEKDTLILNEVDLLVTSALEALDEYMEFTQEKIDEIVAKVSVEAIDAHGRLAKLAVEETKRGVFEDKATKNLFSVEYVIN HMRHLKTVGIISENTVTGITEVADPVGVICAITPVTNPTSTTIFKSLIALKTRNPIVFAFHPNAQNSSKEAARIIKEAAI KSGAPKNCISWIEHPSLDNTNKLMNHPGIATILATGGNAMVKAAYSCGKPALGVGAGNVPAYIEKTANIKTAINDIVMSK SFDNGMVCASEQAAIIDKEIYKEALAEFKKLHTFLCNKEQKQMLETFMFPDGVKLNPYVVGKSAHHIAELAGFEVPLNTN ILLVECKSVGKEEPLTREKLSPVLAILKSNDEVHGVALAKSMVELDGLGHSAVIHTQNDELKKTFGLALKAIRIIVNSPA TFGGIGNVYNRFIPSLTLGCGSYGKNSVSDNVSAIHLLNIKRIGERKNNMQWFKVPSKIYFEKDSIEYLRQMRDVNKVVI ITDRVMVDLGYVKKVTDQLSLRRNHVVYQLFTDVEADPSIETVHKGYELMKSFQPDTIIAIGGGSPMDAAKAMWLFYENP EVDFNDLKQKFMDIRKRAFRYPDLGRKSKLVCIPTTSGTGSEVTPFAVITDNKESKKYPLADYSLTPTVAIIDPALTLTQ PKSVTADTGMDVLTHAVEAYVSVLANDYTDSLAIQAVKMVFKYLKRAFDNGLDFEAREKMHNASTMAGMAFGNAFLGISH SMAHKVGGRYHIPHGRINAILLPHVVLYNGTVPAKLSTWPKYTYYVADEKYMELARAIGLDPKSKEEGVKMFAKACHDLA VSLGINMSFASQKVEEKDFLEHSETLAYLAYEDQCSTANPRLPLVADMKVILENAYYGKLDI >Mature_862_residues MEKDTLILNEVDLLVTSALEALDEYMEFTQEKIDEIVAKVSVEAIDAHGRLAKLAVEETKRGVFEDKATKNLFSVEYVIN HMRHLKTVGIISENTVTGITEVADPVGVICAITPVTNPTSTTIFKSLIALKTRNPIVFAFHPNAQNSSKEAARIIKEAAI KSGAPKNCISWIEHPSLDNTNKLMNHPGIATILATGGNAMVKAAYSCGKPALGVGAGNVPAYIEKTANIKTAINDIVMSK SFDNGMVCASEQAAIIDKEIYKEALAEFKKLHTFLCNKEQKQMLETFMFPDGVKLNPYVVGKSAHHIAELAGFEVPLNTN ILLVECKSVGKEEPLTREKLSPVLAILKSNDEVHGVALAKSMVELDGLGHSAVIHTQNDELKKTFGLALKAIRIIVNSPA TFGGIGNVYNRFIPSLTLGCGSYGKNSVSDNVSAIHLLNIKRIGERKNNMQWFKVPSKIYFEKDSIEYLRQMRDVNKVVI ITDRVMVDLGYVKKVTDQLSLRRNHVVYQLFTDVEADPSIETVHKGYELMKSFQPDTIIAIGGGSPMDAAKAMWLFYENP EVDFNDLKQKFMDIRKRAFRYPDLGRKSKLVCIPTTSGTGSEVTPFAVITDNKESKKYPLADYSLTPTVAIIDPALTLTQ PKSVTADTGMDVLTHAVEAYVSVLANDYTDSLAIQAVKMVFKYLKRAFDNGLDFEAREKMHNASTMAGMAFGNAFLGISH SMAHKVGGRYHIPHGRINAILLPHVVLYNGTVPAKLSTWPKYTYYVADEKYMELARAIGLDPKSKEEGVKMFAKACHDLA VSLGINMSFASQKVEEKDFLEHSETLAYLAYEDQCSTANPRLPLVADMKVILENAYYGKLDI
Specific function: This enzyme has three activities:ADH, ACDH, and PFL- deactivase. In aerobic conditions it acts as a hydrogen peroxide scavenger. The PFL deactivase activity catalyzes the quenching of the pyruvate-formate-lyase catalyst in an iron, NAD, and CoA dependent
COG id: COG1012
COG function: function code C; NAD-dependent aldehyde dehydrogenases
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: In the C-terminal section; belongs to the iron- containing alcohol dehydrogenase family [H]
Homologues:
Organism=Homo sapiens, GI133922590, Length=329, Percent_Identity=31.306990881459, Blast_Score=129, Evalue=1e-29, Organism=Escherichia coli, GI1787493, Length=861, Percent_Identity=46.2253193960511, Blast_Score=777, Evalue=0.0, Organism=Escherichia coli, GI48994951, Length=397, Percent_Identity=35.264483627204, Blast_Score=219, Evalue=4e-58, Organism=Escherichia coli, GI87082107, Length=411, Percent_Identity=33.8199513381995, Blast_Score=205, Evalue=8e-54, Organism=Escherichia coli, GI1789163, Length=402, Percent_Identity=32.5870646766169, Blast_Score=196, Evalue=4e-51, Organism=Escherichia coli, GI1788797, Length=379, Percent_Identity=31.1345646437995, Blast_Score=163, Evalue=5e-41, Organism=Escherichia coli, GI1789386, Length=315, Percent_Identity=26.031746031746, Blast_Score=72, Evalue=1e-13, Organism=Caenorhabditis elegans, GI17537053, Length=279, Percent_Identity=28.673835125448, Blast_Score=130, Evalue=4e-30, Organism=Saccharomyces cerevisiae, GI6321181, Length=418, Percent_Identity=32.0574162679426, Blast_Score=197, Evalue=6e-51, Organism=Drosophila melanogaster, GI24657991, Length=334, Percent_Identity=32.0359281437126, Blast_Score=124, Evalue=3e-28,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR001670 - InterPro: IPR018211 - InterPro: IPR016161 - InterPro: IPR016163 - InterPro: IPR016162 - InterPro: IPR015590 - InterPro: IPR012079 [H]
Pfam domain/function: PF00171 Aldedh; PF00465 Fe-ADH [H]
EC number: =1.1.1.1; =1.2.1.10 [H]
Molecular weight: Translated: 95110; Mature: 95110
Theoretical pI: Translated: 7.26; Mature: 7.26
Prosite motif: PS00913 ADH_IRON_1
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.2 %Cys (Translated Protein) 3.1 %Met (Translated Protein) 4.3 %Cys+Met (Translated Protein) 1.2 %Cys (Mature Protein) 3.1 %Met (Mature Protein) 4.3 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MEKDTLILNEVDLLVTSALEALDEYMEFTQEKIDEIVAKVSVEAIDAHGRLAKLAVEETK CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH RGVFEDKATKNLFSVEYVINHMRHLKTVGIISENTVTGITEVADPVGVICAITPVTNPTS CCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHCCCCEEEEEEECCCCCCH TTIFKSLIALKTRNPIVFAFHPNAQNSSKEAARIIKEAAIKSGAPKNCISWIEHPSLDNT HHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCC NKLMNHPGIATILATGGNAMVKAAYSCGKPALGVGAGNVPAYIEKTANIKTAINDIVMSK HHHHCCCCEEEEEECCCCCEEHHHHHCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHC SFDNGMVCASEQAAIIDKEIYKEALAEFKKLHTFLCNKEQKQMLETFMFPDGVKLNPYVV CCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCEECCEEE GKSAHHIAELAGFEVPLNTNILLVECKSVGKEEPLTREKLSPVLAILKSNDEVHGVALAK CCCHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHH SMVELDGLGHSAVIHTQNDELKKTFGLALKAIRIIVNSPATFGGIGNVYNRFIPSLTLGC HHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCEECC GSYGKNSVSDNVSAIHLLNIKRIGERKNNMQWFKVPSKIYFEKDSIEYLRQMRDVNKVVI CCCCCCCCCCCCCEEEEEEHHHHCCCCCCCEEEECCCCEEECCHHHHHHHHHHCCCEEEE ITDRVMVDLGYVKKVTDQLSLRRNHVVYQLFTDVEADPSIETVHKGYELMKSFQPDTIIA EECHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEE IGGGSPMDAAKAMWLFYENPEVDFNDLKQKFMDIRKRAFRYPDLGRKSKLVCIPTTSGTG ECCCCCCCHHCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCC SEVTPFAVITDNKESKKYPLADYSLTPTVAIIDPALTLTQPKSVTADTGMDVLTHAVEAY CCCCEEEEEECCCCCCCCCCCCCCCCCEEEEECCCEEECCCCCCCCCCCHHHHHHHHHHH VSVLANDYTDSLAIQAVKMVFKYLKRAFDNGLDFEAREKMHNASTMAGMAFGNAFLGISH HHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHCCCH SMAHKVGGRYHIPHGRINAILLPHVVLYNGTVPAKLSTWPKYTYYVADEKYMELARAIGL HHHHHCCCEEECCCCCCCEEEECEEEEECCCCCCCCCCCCCEEEEECCHHHHHHHHHHCC DPKSKEEGVKMFAKACHDLAVSLGINMSFASQKVEEKDFLEHSETLAYLAYEDQCSTANP CCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCC RLPLVADMKVILENAYYGKLDI CCCHHHHHHHHHHCCCCCCCCC >Mature Secondary Structure MEKDTLILNEVDLLVTSALEALDEYMEFTQEKIDEIVAKVSVEAIDAHGRLAKLAVEETK CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH RGVFEDKATKNLFSVEYVINHMRHLKTVGIISENTVTGITEVADPVGVICAITPVTNPTS CCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHCCCCEEEEEEECCCCCCH TTIFKSLIALKTRNPIVFAFHPNAQNSSKEAARIIKEAAIKSGAPKNCISWIEHPSLDNT HHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCC NKLMNHPGIATILATGGNAMVKAAYSCGKPALGVGAGNVPAYIEKTANIKTAINDIVMSK HHHHCCCCEEEEEECCCCCEEHHHHHCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHC SFDNGMVCASEQAAIIDKEIYKEALAEFKKLHTFLCNKEQKQMLETFMFPDGVKLNPYVV CCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCEECCEEE GKSAHHIAELAGFEVPLNTNILLVECKSVGKEEPLTREKLSPVLAILKSNDEVHGVALAK CCCHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHH SMVELDGLGHSAVIHTQNDELKKTFGLALKAIRIIVNSPATFGGIGNVYNRFIPSLTLGC HHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCEECC GSYGKNSVSDNVSAIHLLNIKRIGERKNNMQWFKVPSKIYFEKDSIEYLRQMRDVNKVVI CCCCCCCCCCCCCEEEEEEHHHHCCCCCCCEEEECCCCEEECCHHHHHHHHHHCCCEEEE ITDRVMVDLGYVKKVTDQLSLRRNHVVYQLFTDVEADPSIETVHKGYELMKSFQPDTIIA EECHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEE IGGGSPMDAAKAMWLFYENPEVDFNDLKQKFMDIRKRAFRYPDLGRKSKLVCIPTTSGTG ECCCCCCCHHCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCC SEVTPFAVITDNKESKKYPLADYSLTPTVAIIDPALTLTQPKSVTADTGMDVLTHAVEAY CCCCEEEEEECCCCCCCCCCCCCCCCCEEEEECCCEEECCCCCCCCCCCHHHHHHHHHHH VSVLANDYTDSLAIQAVKMVFKYLKRAFDNGLDFEAREKMHNASTMAGMAFGNAFLGISH HHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHCCCH SMAHKVGGRYHIPHGRINAILLPHVVLYNGTVPAKLSTWPKYTYYVADEKYMELARAIGL HHHHHCCCEEECCCCCCCEEEECEEEEECCCCCCCCCCCCCEEEEECCHHHHHHHHHHCC DPKSKEEGVKMFAKACHDLAVSLGINMSFASQKVEEKDFLEHSETLAYLAYEDQCSTANP CCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCC RLPLVADMKVILENAYYGKLDI CCCHHHHHHHHHHCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 11206551; 11258796 [H]