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The map label for this gene is ycdT [H]

Identifier: 162447044

GI number: 162447044

Start: 163104

End: 164231

Strand: Direct

Name: ycdT [H]

Synonym: ACL_0174

Alternate gene names: 162447044

Gene position: 163104-164231 (Clockwise)

Preceding gene: 162447043

Following gene: 162447045

Centisome position: 10.9

GC content: 27.93

Gene sequence:

>1128_bases
ATGCAAACTGTAAATATAGCTTTATTTGTTTATTCATTTATCTTATTGATTTCTATATATTTATCAAGTGGACAAGTTAG
GTATAAGTTAATGAGTGATAGGCTTTTTAGGTCACTCATTAGAGTAACCCTGCTTCTTTTAATGGTTGATATGATAGCTA
GGTTTGAGGGATTAGACTATTGGTTCTATCCTGTTTTTAGTCGTGTAGGTAATTTTGTATCCTATGGTATAAGTCACTTT
ATATCATCATTATGGTTCTTATATATTCATAATAGAATTTATACAGATACTGAACGAACAAAAAACTTATACAAATACTT
TTTCCTAATTAATTTGGTAAACTGGATTTTACTAATTATCACTCAGTTTACTGGATGGTATTATTATTTGGATAGTTCAA
ATATATATCACAGAGGACCATTATTTTTCTGGACACAATTAGTCAATGCTGTAATTTTACTTAGTTCACTTGTATTACTT
GTATTAAAACGTAAACGTATTGAACAAAACACGATGTTTGCATATATTTTGTTTAGTTTAATTCCGGCGATTGGCTTAAC
TTTACAAATTATATTACCTGGAATTGCCTATATTCCAAATTCAGTGGCTGCTGCACTCATTATTCTATATTTATTCGTTC
AAAACAATAAAGTAAGATATGATCACTTAACCGGCATATTTAATAGAAGGCACATGGATTATTATTTAGAAGATAAACTA
ATAAGTGCAAAGCGCGGTAAACCATTTTCAGCTATTTTATTTGATTTAGATGATTTTAAAGATATCAATGATACATTAGG
TCACTCTGTTGGTGATATTGCGATTAAAAAGACTGCGAAAATACTTGCCAGTGCAAGTAGTAAACATGAATTTTTAGCAC
GTTATGGTGGAGATGAGTTTTTGATCATAACAAGTAAGTATGAAAAACAAGATATTGATGAAATAGTAAAAAGGGTCTAT
GATGGTATTAATCAGTTTAATAAAATCAATGATAAATTTAAACTAAGTATTAGTCATGGTTATTATGTATATCATAAGGA
TTTAAATTTAAGTCGAGATCAATTCATTGATGTTGTAGATAGAAATATGTATGATAATAAAATAAAAAAGAAAAATTCTA
GAGTATGA

Upstream 100 bases:

>100_bases
TATTTAAAACGTTAGATTATAGTTATTGACAGTTTTAAATTTAAGGTATGTTAAAATATTTGTTGTGTGATAAATAATTA
CATAGATTAGGGAGAAATCT

Downstream 100 bases:

>100_bases
TACAAAACACGATATTAAATCAATTAAATGAAAAAGGATTTGTAGTATCTAAAATAAGAGGGGTAAGTATGTGGCCCTTC
TTTAATCAAAAAAATACGCA

Product: GGDEF domain-containing protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MQTVNIALFVYSFILLISIYLSSGQVRYKLMSDRLFRSLIRVTLLLLMVDMIARFEGLDYWFYPVFSRVGNFVSYGISHF
ISSLWFLYIHNRIYTDTERTKNLYKYFFLINLVNWILLIITQFTGWYYYLDSSNIYHRGPLFFWTQLVNAVILLSSLVLL
VLKRKRIEQNTMFAYILFSLIPAIGLTLQIILPGIAYIPNSVAAALIILYLFVQNNKVRYDHLTGIFNRRHMDYYLEDKL
ISAKRGKPFSAILFDLDDFKDINDTLGHSVGDIAIKKTAKILASASSKHEFLARYGGDEFLIITSKYEKQDIDEIVKRVY
DGINQFNKINDKFKLSISHGYYVYHKDLNLSRDQFIDVVDRNMYDNKIKKKNSRV

Sequences:

>Translated_375_residues
MQTVNIALFVYSFILLISIYLSSGQVRYKLMSDRLFRSLIRVTLLLLMVDMIARFEGLDYWFYPVFSRVGNFVSYGISHF
ISSLWFLYIHNRIYTDTERTKNLYKYFFLINLVNWILLIITQFTGWYYYLDSSNIYHRGPLFFWTQLVNAVILLSSLVLL
VLKRKRIEQNTMFAYILFSLIPAIGLTLQIILPGIAYIPNSVAAALIILYLFVQNNKVRYDHLTGIFNRRHMDYYLEDKL
ISAKRGKPFSAILFDLDDFKDINDTLGHSVGDIAIKKTAKILASASSKHEFLARYGGDEFLIITSKYEKQDIDEIVKRVY
DGINQFNKINDKFKLSISHGYYVYHKDLNLSRDQFIDVVDRNMYDNKIKKKNSRV
>Mature_375_residues
MQTVNIALFVYSFILLISIYLSSGQVRYKLMSDRLFRSLIRVTLLLLMVDMIARFEGLDYWFYPVFSRVGNFVSYGISHF
ISSLWFLYIHNRIYTDTERTKNLYKYFFLINLVNWILLIITQFTGWYYYLDSSNIYHRGPLFFWTQLVNAVILLSSLVLL
VLKRKRIEQNTMFAYILFSLIPAIGLTLQIILPGIAYIPNSVAAALIILYLFVQNNKVRYDHLTGIFNRRHMDYYLEDKL
ISAKRGKPFSAILFDLDDFKDINDTLGHSVGDIAIKKTAKILASASSKHEFLARYGGDEFLIITSKYEKQDIDEIVKRVY
DGINQFNKINDKFKLSISHGYYVYHKDLNLSRDQFIDVVDRNMYDNKIKKKNSRV

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=169, Percent_Identity=28.9940828402367, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1786584, Length=154, Percent_Identity=30.5194805194805, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI145693134, Length=170, Percent_Identity=27.6470588235294, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI87081974, Length=155, Percent_Identity=32.258064516129, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787816, Length=181, Percent_Identity=32.0441988950276, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI87081881, Length=159, Percent_Identity=27.0440251572327, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 44086; Mature: 44086

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTVNIALFVYSFILLISIYLSSGQVRYKLMSDRLFRSLIRVTLLLLMVDMIARFEGLDY
CCEEHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
WFYPVFSRVGNFVSYGISHFISSLWFLYIHNRIYTDTERTKNLYKYFFLINLVNWILLII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
TQFTGWYYYLDSSNIYHRGPLFFWTQLVNAVILLSSLVLLVLKRKRIEQNTMFAYILFSL
HHHCCEEEEEECCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IPAIGLTLQIILPGIAYIPNSVAAALIILYLFVQNNKVRYDHLTGIFNRRHMDYYLEDKL
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHCCCCCHHHHHHH
ISAKRGKPFSAILFDLDDFKDINDTLGHSVGDIAIKKTAKILASASSKHEFLARYGGDEF
HHHCCCCCCEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEE
LIITSKYEKQDIDEIVKRVYDGINQFNKINDKFKLSISHGYYVYHKDLNLSRDQFIDVVD
EEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEEEECCCCCCHHHHHHHHH
RNMYDNKIKKKNSRV
CCHHHHHHHHCCCCC
>Mature Secondary Structure
MQTVNIALFVYSFILLISIYLSSGQVRYKLMSDRLFRSLIRVTLLLLMVDMIARFEGLDY
CCEEHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
WFYPVFSRVGNFVSYGISHFISSLWFLYIHNRIYTDTERTKNLYKYFFLINLVNWILLII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
TQFTGWYYYLDSSNIYHRGPLFFWTQLVNAVILLSSLVLLVLKRKRIEQNTMFAYILFSL
HHHCCEEEEEECCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IPAIGLTLQIILPGIAYIPNSVAAALIILYLFVQNNKVRYDHLTGIFNRRHMDYYLEDKL
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHCCCCCHHHHHHH
ISAKRGKPFSAILFDLDDFKDINDTLGHSVGDIAIKKTAKILASASSKHEFLARYGGDEF
HHHCCCCCCEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEE
LIITSKYEKQDIDEIVKRVYDGINQFNKINDKFKLSISHGYYVYHKDLNLSRDQFIDVVD
EEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEEEECCCCCCHHHHHHHHH
RNMYDNKIKKKNSRV
CCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]