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The map label for this gene is apbE [H]

Identifier: 162446940

GI number: 162446940

Start: 63791

End: 64873

Strand: Direct

Name: apbE [H]

Synonym: ACL_0054

Alternate gene names: 162446940

Gene position: 63791-64873 (Clockwise)

Preceding gene: 162446939

Following gene: 162446941

Centisome position: 4.26

GC content: 31.86

Gene sequence:

>1083_bases
ATGAAAAAAATATGGGCAGTTATCAACTTAATATTAATCAGTGTTCTTTTTTCTGCTTGTACACAACAAGCAATTGATAA
CAGTTTAGCCATATCATCTATGGATACATTTATTGGTGGCACAGTACATGCACCAAAAGCTGTTTGGGATAAAGCGGATA
AAAAACTAAAAGAAATGTATATGCTCTACCACGAGTTAACCGATAACTATAGAGGTTATGAGGGTGTACAAGGTGTTTAC
TATATTAATAATTTAGTAGAAACAACGAAAACTGACCAAACAGTTGAGATTAAAAAAGAACTTTATGATCTATTAAGTTT
AGGTGTAGAACTCTATGAATTTACAGATGGATACTTTGATATGTCCATTGGTAAGATCATCGATGTTTGGAAAAACTTAA
TTGATGAAAGTGATTATACGGGTCAAGAAATACCTAAAGAAGATATCGATTATGCGATAAACTTAGTGGATGCTATTGAT
ATCATTGAAGATCCGGTGATTTTAAGTGAAGTAGATAATAAATATTACGTGACACTTAAATATGGTGCAAAACTAGACTT
AGGTGCGCTTGCTAAAGGGTATGTCACTCAGTTAGCAGCAGATTATTTAACGGATTTAGGTTTAAGTACCTATCTCATTA
GTGGTGGTGGCTCATCCATGTTATATGGTGAAGGTAATCCAAAAACTGAAGATGGTAGTTACAGAACAGGACTTATTAAT
CCACAAGATGTTATTGATAACTTAGATATTATTGGATATCGACCTAAAAATTACGGTATATATACAGCTAAAAATTATAA
CTTCACAACATCAGGAAGTTATAATCAATTTATCTTAAGTGAAGGTGTGATGTATCATCACATTGTCTCACCTAAAACAA
AACGTCCGGTAAATCATTATCTAACACTTTCTATTGTTGGAACAGATGCAGGATTTAATGATGGTTTATCCACAGCATTA
TTTAGTATGCCACCTGAAGTGTTAGAACAGTTTTTAGAAACGAATGGTTATGAGGTCTATACATATTTATTTAATAATAC
TTATAAATCCTATAATCAATCTGAAAACTTTAGACCACTTTAA

Upstream 100 bases:

>100_bases
TAAAGGATTACCAATTCTACTCATTATACTGGGCTTAATTGCTTTAGTATTTAGTGGGTTAGCAGGATTATTTTAAAAAC
ATATAAAAAGGAAAAAGAAC

Downstream 100 bases:

>100_bases
TTAGGTGATGATATGAAGAAAAGAGATTATATCATCATTGGTGGACTATTAGTTGTTGTAGGTATACTTTATATTTGGGT
TACATATTTTGGTGCCCCAA

Product: thiamine biosynthesis lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MKKIWAVINLILISVLFSACTQQAIDNSLAISSMDTFIGGTVHAPKAVWDKADKKLKEMYMLYHELTDNYRGYEGVQGVY
YINNLVETTKTDQTVEIKKELYDLLSLGVELYEFTDGYFDMSIGKIIDVWKNLIDESDYTGQEIPKEDIDYAINLVDAID
IIEDPVILSEVDNKYYVTLKYGAKLDLGALAKGYVTQLAADYLTDLGLSTYLISGGGSSMLYGEGNPKTEDGSYRTGLIN
PQDVIDNLDIIGYRPKNYGIYTAKNYNFTTSGSYNQFILSEGVMYHHIVSPKTKRPVNHYLTLSIVGTDAGFNDGLSTAL
FSMPPEVLEQFLETNGYEVYTYLFNNTYKSYNQSENFRPL

Sequences:

>Translated_360_residues
MKKIWAVINLILISVLFSACTQQAIDNSLAISSMDTFIGGTVHAPKAVWDKADKKLKEMYMLYHELTDNYRGYEGVQGVY
YINNLVETTKTDQTVEIKKELYDLLSLGVELYEFTDGYFDMSIGKIIDVWKNLIDESDYTGQEIPKEDIDYAINLVDAID
IIEDPVILSEVDNKYYVTLKYGAKLDLGALAKGYVTQLAADYLTDLGLSTYLISGGGSSMLYGEGNPKTEDGSYRTGLIN
PQDVIDNLDIIGYRPKNYGIYTAKNYNFTTSGSYNQFILSEGVMYHHIVSPKTKRPVNHYLTLSIVGTDAGFNDGLSTAL
FSMPPEVLEQFLETNGYEVYTYLFNNTYKSYNQSENFRPL
>Mature_360_residues
MKKIWAVINLILISVLFSACTQQAIDNSLAISSMDTFIGGTVHAPKAVWDKADKKLKEMYMLYHELTDNYRGYEGVQGVY
YINNLVETTKTDQTVEIKKELYDLLSLGVELYEFTDGYFDMSIGKIIDVWKNLIDESDYTGQEIPKEDIDYAINLVDAID
IIEDPVILSEVDNKYYVTLKYGAKLDLGALAKGYVTQLAADYLTDLGLSTYLISGGGSSMLYGEGNPKTEDGSYRTGLIN
PQDVIDNLDIIGYRPKNYGIYTAKNYNFTTSGSYNQFILSEGVMYHHIVSPKTKRPVNHYLTLSIVGTDAGFNDGLSTAL
FSMPPEVLEQFLETNGYEVYTYLFNNTYKSYNQSENFRPL

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=248, Percent_Identity=21.7741935483871, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE [H]

EC number: NA

Molecular weight: Translated: 40587; Mature: 40587

Theoretical pI: Translated: 4.34; Mature: 4.34

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIWAVINLILISVLFSACTQQAIDNSLAISSMDTFIGGTVHAPKAVWDKADKKLKEMY
CHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
MLYHELTDNYRGYEGVQGVYYINNLVETTKTDQTVEIKKELYDLLSLGVELYEFTDGYFD
HHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCHHHHHHCCCEEH
MSIGKIIDVWKNLIDESDYTGQEIPKEDIDYAINLVDAIDIIEDPVILSEVDNKYYVTLK
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEEE
YGAKLDLGALAKGYVTQLAADYLTDLGLSTYLISGGGSSMLYGEGNPKTEDGSYRTGLIN
ECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEECCCCCCCCCCCEEECCCC
PQDVIDNLDIIGYRPKNYGIYTAKNYNFTTSGSYNQFILSEGVMYHHIVSPKTKRPVNHY
HHHHHCCCCEEEECCCCCCEEEECCCCEECCCCCCCHHHHCCCEEEEECCCCCCCCCCEE
LTLSIVGTDAGFNDGLSTALFSMPPEVLEQFLETNGYEVYTYLFNNTYKSYNQSENFRPL
EEEEEEECCCCCCCCHHHHHHCCCHHHHHHHHHCCCCEEEEEEECCHHHHCCCCCCCCCC
>Mature Secondary Structure
MKKIWAVINLILISVLFSACTQQAIDNSLAISSMDTFIGGTVHAPKAVWDKADKKLKEMY
CHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
MLYHELTDNYRGYEGVQGVYYINNLVETTKTDQTVEIKKELYDLLSLGVELYEFTDGYFD
HHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCHHHHHHCCCEEH
MSIGKIIDVWKNLIDESDYTGQEIPKEDIDYAINLVDAIDIIEDPVILSEVDNKYYVTLK
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEEE
YGAKLDLGALAKGYVTQLAADYLTDLGLSTYLISGGGSSMLYGEGNPKTEDGSYRTGLIN
ECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEECCCCCCCCCCCEEECCCC
PQDVIDNLDIIGYRPKNYGIYTAKNYNFTTSGSYNQFILSEGVMYHHIVSPKTKRPVNHY
HHHHHCCCCEEEECCCCCCEEEECCCCEECCCCCCCHHHHCCCEEEEECCCCCCCCCCEE
LTLSIVGTDAGFNDGLSTALFSMPPEVLEQFLETNGYEVYTYLFNNTYKSYNQSENFRPL
EEEEEEECCCCCCCCHHHHHHCCCHHHHHHHHHCCCCEEEEEEECCHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]