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The map label for this gene is cls [C]

Identifier: 162446912

GI number: 162446912

Start: 29333

End: 30841

Strand: Direct

Name: cls [C]

Synonym: ACL_0025

Alternate gene names: 162446912

Gene position: 29333-30841 (Clockwise)

Preceding gene: 162446911

Following gene: 162446913

Centisome position: 1.96

GC content: 30.88

Gene sequence:

>1509_bases
ATGAGAAGGTTTATAAATTTACTAACGAATAGACTTACAATCATTGGACTAATTTTGTTACTTCAAATTGGTATTTTATG
GTGGTTTGTCTATGAGTTTGCAGCAACCTGGTATTACCTTCATCTTGTATCTATGATACTTGGTGTGATATTAAGTTTAT
ATATTATTACAACCGATGAAAATCCGATGTTTAAATTGATGTGGGTGGTATTTATGATTGTGCTACCTGTGTTTGGTGTA
CTCTTTTACTTATACGCCAAAACAGAAAGACTATCTATCTCTTCTTCATCAACCATGAGGCGGATGCAAAAGGTAAGAGA
AAATGAGATGCGAAAAATTAAATCCGTTTTTGACCCTAAGTACTTAAAACAACAAAAATACCTAACCAATTTAAATTTCC
CATCTTTTAAAAACACATCTTCCATTTTTTTAGGTAGTGGTGAAGAAAAACAAACTGAACTTTTAAGAGAACTTAAAAAA
GCAAAACACTTCATCTTCATGGAATACTTTATTATCACGATTTCTAAGATGTGGGATGAAATATTACAGGTTCTTATAGA
AAAACAAAAACAAGGTGTGGAAATCAGAATCATGTATGATGATTTTGGTTCTGCAACCAAACTTCCATTTTATTATCATA
AAAAGTTAACTAAAATGGGAATTAAAGTTGTTCGTTTTAACCCGATGAAACTACACGTGAACTTTGCTATGAATTTTAGA
GATCATAGAAAAATTGTAGTTATAGATAATAGAGTAGCCTTTACTGGTGGATTTAATATAGGAGATGAATATACAAATAA
GAAAAAAGTATTTGGTCACTGGAATGATGCTGGTATCATGATTGAAGGTGAAGCTGTTTGGAGTATGACAATGCTCTTTT
TAGAAAACTGGTCGTTTTCTAAAAAAGATGATACAACTGATTTTTCAAAATATAACATCAAATATCCACCAATTATAAAT
GATGCAATCTATATTCCGTTTGGAGATATCCCGGTAGATAATAATCTAACAGCTAAAAATATATATCTACACTTAATTAA
TGATGCACAGCAGACAATTTATATTACTGCACCGTACTTAATTTTAGATAATGAAATCGCAACCGCACTTAAACTAGCCG
CTCAAAGTGGTGTCGATGTACACATCATTATGCCAAGTATACCGGATAAGAGATTTGTATATATGGTGTCAAAATCATAT
GCGGAAGAACTTGTTCATAGCGGAGTTAAAATTTATAGATATAAACCAGGGTTTATCCACTCTAAGATGATTATTTGTGA
TAAAGAAGCAGCGATGATTGGATCAAGTAATTTAGACTTTAGAAGCTTATACATGCACTTAGAAAACAACTTATGGTTGA
ATGATATAAAAACTATTTCTAATATGGTTAATTATTATGAATACACACTCAAAGAAAGTGAACGTATTACAATCAAAGAC
TTCACTAAGAGAAATATTATCTATAGAGTAGTACAGGCAGTATTAAAGGGTTTCTCGCCATTATTATAA

Upstream 100 bases:

>100_bases
TATTTAATGAGTTATGTGATTTATGTTGTAAGTACCATCGCATCTATTATGTTAATGCCCCTGATTCAAAGTTTCTTTTA
ATTATTAAAAGGAGTTTAAA

Downstream 100 bases:

>100_bases
AATGAATATATTAAGCTATTATAAAGAAATAACCTTATACGAGGGGATTTAAGAATGGGAAAAGATTACAAATTTATTAA
AAACGGAATAAAGGTTGCAC

Product: phosphatidylserine/phosphatidylglycerophosphate synthase

Products: Biphosphatidylglycerol; Glycerol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 502; Mature: 502

Protein sequence:

>502_residues
MRRFINLLTNRLTIIGLILLLQIGILWWFVYEFAATWYYLHLVSMILGVILSLYIITTDENPMFKLMWVVFMIVLPVFGV
LFYLYAKTERLSISSSSTMRRMQKVRENEMRKIKSVFDPKYLKQQKYLTNLNFPSFKNTSSIFLGSGEEKQTELLRELKK
AKHFIFMEYFIITISKMWDEILQVLIEKQKQGVEIRIMYDDFGSATKLPFYYHKKLTKMGIKVVRFNPMKLHVNFAMNFR
DHRKIVVIDNRVAFTGGFNIGDEYTNKKKVFGHWNDAGIMIEGEAVWSMTMLFLENWSFSKKDDTTDFSKYNIKYPPIIN
DAIYIPFGDIPVDNNLTAKNIYLHLINDAQQTIYITAPYLILDNEIATALKLAAQSGVDVHIIMPSIPDKRFVYMVSKSY
AEELVHSGVKIYRYKPGFIHSKMIICDKEAAMIGSSNLDFRSLYMHLENNLWLNDIKTISNMVNYYEYTLKESERITIKD
FTKRNIIYRVVQAVLKGFSPLL

Sequences:

>Translated_502_residues
MRRFINLLTNRLTIIGLILLLQIGILWWFVYEFAATWYYLHLVSMILGVILSLYIITTDENPMFKLMWVVFMIVLPVFGV
LFYLYAKTERLSISSSSTMRRMQKVRENEMRKIKSVFDPKYLKQQKYLTNLNFPSFKNTSSIFLGSGEEKQTELLRELKK
AKHFIFMEYFIITISKMWDEILQVLIEKQKQGVEIRIMYDDFGSATKLPFYYHKKLTKMGIKVVRFNPMKLHVNFAMNFR
DHRKIVVIDNRVAFTGGFNIGDEYTNKKKVFGHWNDAGIMIEGEAVWSMTMLFLENWSFSKKDDTTDFSKYNIKYPPIIN
DAIYIPFGDIPVDNNLTAKNIYLHLINDAQQTIYITAPYLILDNEIATALKLAAQSGVDVHIIMPSIPDKRFVYMVSKSY
AEELVHSGVKIYRYKPGFIHSKMIICDKEAAMIGSSNLDFRSLYMHLENNLWLNDIKTISNMVNYYEYTLKESERITIKD
FTKRNIIYRVVQAVLKGFSPLL
>Mature_502_residues
MRRFINLLTNRLTIIGLILLLQIGILWWFVYEFAATWYYLHLVSMILGVILSLYIITTDENPMFKLMWVVFMIVLPVFGV
LFYLYAKTERLSISSSSTMRRMQKVRENEMRKIKSVFDPKYLKQQKYLTNLNFPSFKNTSSIFLGSGEEKQTELLRELKK
AKHFIFMEYFIITISKMWDEILQVLIEKQKQGVEIRIMYDDFGSATKLPFYYHKKLTKMGIKVVRFNPMKLHVNFAMNFR
DHRKIVVIDNRVAFTGGFNIGDEYTNKKKVFGHWNDAGIMIEGEAVWSMTMLFLENWSFSKKDDTTDFSKYNIKYPPIIN
DAIYIPFGDIPVDNNLTAKNIYLHLINDAQQTIYITAPYLILDNEIATALKLAAQSGVDVHIIMPSIPDKRFVYMVSKSY
AEELVHSGVKIYRYKPGFIHSKMIICDKEAAMIGSSNLDFRSLYMHLENNLWLNDIKTISNMVNYYEYTLKESERITIKD
FTKRNIIYRVVQAVLKGFSPLL

Specific function: Catalyzes The Reversible Phosphatidyl Group Transfer From One Phosphatidylglycerol Molecule To Another To Form Cardiolipin (Cl) (Diphosphatidylglycerol) And Glycerol. Affects Resistance To The Gyrase Inhibitor Novobiocin. [C]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:
GO:0003824: Phosphatidylserine/phosphatidylglycerophosphate synthase
GO:0008152: Phosphatidylserine/phosphatidylglycerophosphate synthase
GO:0016740: Phosphatidylserine/phosphatidylglycerophosphate synthase

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=460, Percent_Identity=25.4347826086956, Blast_Score=169, Evalue=5e-43,
Organism=Escherichia coli, GI1787007, Length=335, Percent_Identity=26.2686567164179, Blast_Score=95, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 58929; Mature: 58929

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRFINLLTNRLTIIGLILLLQIGILWWFVYEFAATWYYLHLVSMILGVILSLYIITTDE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCC
NPMFKLMWVVFMIVLPVFGVLFYLYAKTERLSISSSSTMRRMQKVRENEMRKIKSVFDPK
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCHH
YLKQQKYLTNLNFPSFKNTSSIFLGSGEEKQTELLRELKKAKHFIFMEYFIITISKMWDE
HHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILQVLIEKQKQGVEIRIMYDDFGSATKLPFYYHKKLTKMGIKVVRFNPMKLHVNFAMNFR
HHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHCCEEEEEECCEEEEEEEEECCC
DHRKIVVIDNRVAFTGGFNIGDEYTNKKKVFGHWNDAGIMIEGEAVWSMTMLFLENWSFS
CCCEEEEEECEEEEECCCCCCCCCCCCCCEEEEECCCCEEEECHHHHHHHHHHHHCCCCC
KKDDTTDFSKYNIKYPPIINDAIYIPFGDIPVDNNLTAKNIYLHLINDAQQTIYITAPYL
CCCCCCCCCEEEEECCCCCCCEEEEECCCCCCCCCCCCCEEEEEEECCCCEEEEEEECEE
ILDNEIATALKLAAQSGVDVHIIMPSIPDKRFVYMVSKSYAEELVHSGVKIYRYKPGFIH
EECCHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEHHHHHHHHHHHCCEEEEEECCCCEE
SKMIICDKEAAMIGSSNLDFRSLYMHLENNLWLNDIKTISNMVNYYEYTLKESERITIKD
CEEEEECCCHHEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEH
FTKRNIIYRVVQAVLKGFSPLL
HHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRRFINLLTNRLTIIGLILLLQIGILWWFVYEFAATWYYLHLVSMILGVILSLYIITTDE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCC
NPMFKLMWVVFMIVLPVFGVLFYLYAKTERLSISSSSTMRRMQKVRENEMRKIKSVFDPK
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCHH
YLKQQKYLTNLNFPSFKNTSSIFLGSGEEKQTELLRELKKAKHFIFMEYFIITISKMWDE
HHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILQVLIEKQKQGVEIRIMYDDFGSATKLPFYYHKKLTKMGIKVVRFNPMKLHVNFAMNFR
HHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHCCEEEEEECCEEEEEEEEECCC
DHRKIVVIDNRVAFTGGFNIGDEYTNKKKVFGHWNDAGIMIEGEAVWSMTMLFLENWSFS
CCCEEEEEECEEEEECCCCCCCCCCCCCCEEEEECCCCEEEECHHHHHHHHHHHHCCCCC
KKDDTTDFSKYNIKYPPIINDAIYIPFGDIPVDNNLTAKNIYLHLINDAQQTIYITAPYL
CCCCCCCCCEEEEECCCCCCCEEEEECCCCCCCCCCCCCEEEEEEECCCCEEEEEEECEE
ILDNEIATALKLAAQSGVDVHIIMPSIPDKRFVYMVSKSYAEELVHSGVKIYRYKPGFIH
EECCHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEHHHHHHHHHHHCCEEEEEECCCCEE
SKMIICDKEAAMIGSSNLDFRSLYMHLENNLWLNDIKTISNMVNYYEYTLKESERITIKD
CEEEEECCCHHEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEH
FTKRNIIYRVVQAVLKGFSPLL
HHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Phosphatidylglycerol [C]

Specific reaction: 2 Phosphatidylglycerol = Biphosphatidylglycerol + Glycerol. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12397186; 2761387 [H]