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The map label for this gene is fcl [H]

Identifier: 162420075

GI number: 162420075

Start: 1307440

End: 1308405

Strand: Direct

Name: fcl [H]

Synonym: YpAngola_A1253

Alternate gene names: 162420075

Gene position: 1307440-1308405 (Clockwise)

Preceding gene: 162418806

Following gene: 162420943

Centisome position: 29.03

GC content: 41.72

Gene sequence:

>966_bases
ATGGATAAGAAACGCGTATTTATTGCCGGGCATCGTGGCATGGTGGGTTCTGCTATTGTACGTCAACTTGAAAACCGTAA
TGATATTGAGTTGATCATTAGGGATCGTACTGAACTTGACCTCATGTCTCAATCCGCTGTGCAAAAATTTTTTGCTACTG
AAAAAATTGATGAAATCTATTTGGCTGCGGCAAAAGTGGGGGGGATTCAGGCCAATAATAATTATCCGGCAGAGTTCATC
TACCAAAACTTAATGATCGAGTGCAATATTATTCACGCGGCTCATTTAGCTGGCATTCAAAAATTATTATTTTTGGGGGC
TTCTTGTATTTATCCAAAATTGGCTGCACAACCAATGACAGAGGAGGCTCTGTTAACTGGCGTCTTGGAACCAACGAATG
AACCTTATGCCATCGCCAAGATAGCCGGTATCAAACTGTGCGAATCTTATAATCGTCAATATGGTCGCGATTATCGCAGT
GTTATGCCAACCAACCTTTATGGTGAAAATGACAATTTTCACCCCGAAAATTCCCATGTCATTCCTGCCTTATTACGTCG
CTTCCATGAGGCTAAAATTCGTAATGATAAGGAAATGGTTGTGTGGGGAACGGGTAAACCAATGCGTGAGTTCCTGCATG
TAGATGATATGGCTGCTGCCAGTGTGCATGTCATGGAGCTGTCTGATCAAATTTATCAAACCAATACTCAACCAATGCTT
TCGCATATTAATGTCGGAACGGGTGTGGATTGCACTATTCGTGAATTGGCAGAAACTATGGCTAAAGTTGTTGGTTTCAC
CGGAAATTTAGTTTTTGATTCAACTAAGCCGGACGGAACACCACGAAAATTGATGGACGTAAGCCGCTTGGCTAAACTCG
GGTGGTGTTATCAGATTTCGCTTGAAGTAGGTTTAACGATGACTTATCAATGGTTCTTGGCTCATCAGAATAACTTCAGA
AAATAG

Upstream 100 bases:

>100_bases
GTGCTGAAATGGTCGCTAGTGACTTAGAGCAGGCTAAACAGCATGCATTGTTAAAAGCTAATGGTTTTGACGTATCCATT
TCGTTGGAGAATTAATACAA

Downstream 100 bases:

>100_bases
TTTCATTTGCTATTTGCTATTTGCTATTTGCTATTTGGATATGGCGACTCTTAAGTTGGTTAATTAAGTTTTAACTTAAT
AAGTAAAAAATATTATCGGG

Product: GDP-L-fucose synthetase

Products: NA

Alternate protein names: GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MDKKRVFIAGHRGMVGSAIVRQLENRNDIELIIRDRTELDLMSQSAVQKFFATEKIDEIYLAAAKVGGIQANNNYPAEFI
YQNLMIECNIIHAAHLAGIQKLLFLGASCIYPKLAAQPMTEEALLTGVLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
VMPTNLYGENDNFHPENSHVIPALLRRFHEAKIRNDKEMVVWGTGKPMREFLHVDDMAAASVHVMELSDQIYQTNTQPML
SHINVGTGVDCTIRELAETMAKVVGFTGNLVFDSTKPDGTPRKLMDVSRLAKLGWCYQISLEVGLTMTYQWFLAHQNNFR
K

Sequences:

>Translated_321_residues
MDKKRVFIAGHRGMVGSAIVRQLENRNDIELIIRDRTELDLMSQSAVQKFFATEKIDEIYLAAAKVGGIQANNNYPAEFI
YQNLMIECNIIHAAHLAGIQKLLFLGASCIYPKLAAQPMTEEALLTGVLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
VMPTNLYGENDNFHPENSHVIPALLRRFHEAKIRNDKEMVVWGTGKPMREFLHVDDMAAASVHVMELSDQIYQTNTQPML
SHINVGTGVDCTIRELAETMAKVVGFTGNLVFDSTKPDGTPRKLMDVSRLAKLGWCYQISLEVGLTMTYQWFLAHQNNFR
K
>Mature_321_residues
MDKKRVFIAGHRGMVGSAIVRQLENRNDIELIIRDRTELDLMSQSAVQKFFATEKIDEIYLAAAKVGGIQANNNYPAEFI
YQNLMIECNIIHAAHLAGIQKLLFLGASCIYPKLAAQPMTEEALLTGVLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
VMPTNLYGENDNFHPENSHVIPALLRRFHEAKIRNDKEMVVWGTGKPMREFLHVDDMAAASVHVMELSDQIYQTNTQPML
SHINVGTGVDCTIRELAETMAKVVGFTGNLVFDSTKPDGTPRKLMDVSRLAKLGWCYQISLEVGLTMTYQWFLAHQNNFR
K

Specific function: Two step NADP-dependent conversion of GDP-4-dehydro-6- deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fucose synthase family [H]

Homologues:

Organism=Homo sapiens, GI4507709, Length=289, Percent_Identity=28.719723183391, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI7657641, Length=348, Percent_Identity=22.7011494252874, Blast_Score=76, Evalue=4e-14,
Organism=Escherichia coli, GI1788365, Length=320, Percent_Identity=75, Blast_Score=524, Evalue=1e-150,
Organism=Escherichia coli, GI48994969, Length=353, Percent_Identity=22.9461756373938, Blast_Score=73, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17554428, Length=322, Percent_Identity=27.9503105590062, Blast_Score=147, Evalue=8e-36,
Organism=Drosophila melanogaster, GI19922778, Length=327, Percent_Identity=30.5810397553517, Blast_Score=166, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.271 [H]

Molecular weight: Translated: 36273; Mature: 36273

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKKRVFIAGHRGMVGSAIVRQLENRNDIELIIRDRTELDLMSQSAVQKFFATEKIDEIY
CCCCEEEEECCCCCHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
LAAAKVGGIQANNNYPAEFIYQNLMIECNIIHAAHLAGIQKLLFLGASCIYPKLAAQPMT
HHHHHHCCEECCCCCCHHHHHHHHEEEEEEEHHHHHHHHHHHHHHCCHHHCHHHHCCCCC
EEALLTGVLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRSVMPTNLYGENDNFHPENSHV
HHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCC
IPALLRRFHEAKIRNDKEMVVWGTGKPMREFLHVDDMAAASVHVMELSDQIYQTNTQPML
HHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHCCHHHHHEEEEHHHHHHHHCCCCHHH
SHINVGTGVDCTIRELAETMAKVVGFTGNLVFDSTKPDGTPRKLMDVSRLAKLGWCYQIS
HHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCEEEEE
LEVGLTMTYQWFLAHQNNFRK
EEECEEEEEEEEEECCCCCCC
>Mature Secondary Structure
MDKKRVFIAGHRGMVGSAIVRQLENRNDIELIIRDRTELDLMSQSAVQKFFATEKIDEIY
CCCCEEEEECCCCCHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
LAAAKVGGIQANNNYPAEFIYQNLMIECNIIHAAHLAGIQKLLFLGASCIYPKLAAQPMT
HHHHHHCCEECCCCCCHHHHHHHHEEEEEEEHHHHHHHHHHHHHHCCHHHCHHHHCCCCC
EEALLTGVLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRSVMPTNLYGENDNFHPENSHV
HHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCC
IPALLRRFHEAKIRNDKEMVVWGTGKPMREFLHVDDMAAASVHVMELSDQIYQTNTQPML
HHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHCCHHHHHEEEEHHHHHHHHCCCCHHH
SHINVGTGVDCTIRELAETMAKVVGFTGNLVFDSTKPDGTPRKLMDVSRLAKLGWCYQIS
HHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCEEEEE
LEVGLTMTYQWFLAHQNNFRK
EEECEEEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7815923; 8759852; 9097040; 9278503; 9473059; 9862812 [H]