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The map label for this gene is por [H]

Identifier: 162149046

GI number: 162149046

Start: 3365766

End: 3367061

Strand: Direct

Name: por [H]

Synonym: GDI_3276

Alternate gene names: 162149046

Gene position: 3365766-3367061 (Clockwise)

Preceding gene: 162149039

Following gene: 162149047

Centisome position: 85.34

GC content: 70.29

Gene sequence:

>1296_bases
GTGATGGACGACGCGCGCGAACGTGCCAAGGCCGAGTCCCTGCGGGCGCAGGACGGCCTTTACACCCTGACCGAATGCCC
GGCGGACGCGCCGGATACCGTCCGCGTCATCGGCTCGATCGTCGAATACCTGCACGCCCCCGACGACCGGGCGGCCGCCA
TCCGCCGCCTGGCCGACCCGGCCATCCGCATCGTCAGCCTGACCGTGACCGAAGGCGGATATTACGTGGACGAATCCGGG
CAGTTCCAGATCGACCACCCCACGATCGCCGAGGACCTGGCCCGCGACGTGCCGCACACCGTCTTCGGCCTGGCGACCGA
GGCGCTGCGCCTGCGCCGCGATTCCGGCGCCGGCCCGTTCACCATCCTGTCCTGCGACAACCTGCCACATAACGGCCATG
TGGCGCGCACCGCCTTCCTGTCCTGGGCCCGCGCCCGCGACGCGGACCTGGCCGCCTGGGTCGAGGCCCACGTCACCTTT
CCCTGCACGATGGTGGACCGTATCACCCCCGCGGTGCGCGACCCCGACATCGCGCGCCTGGACGCCGCCAGCGGCATCGC
GGACCGCGCCCCGGTCTTCTGCGAGGATTTCATCCAGTGGGTGGTCGAGGACACGTTCTGCGCCGGCCGCCCGCCGCTGG
AACAGGTGGGCGTGCTGTTCACCGGCGACGTCGCCCCCTACGAGCGGGTCAAGCTGCGCATGCTCAATGCCTCGCATTCC
ATGCTGGGGCTGCCGGGCGTGCTGATGGGATACCGCATCGTCAGCGACATCATGCACAACCCCGATGTCATCGAACTGCT
GGAACGCTATCTGGGCCTCGACGCCGAACCGCTGCTGCAGGCCCCGCCGGGGATGGAACTGCACGAGTACGGCGCGCTGC
TGCTGCGCCGGTTCCGCAACCAGGCGATCAGCGACCAGTTGCTGCGCATCGCGTCGGACAGTGCGTCGAAACTGCCGGTC
TTCGTCCGCGACACCGCCGAGGGCACGCTGGCCCGGGGCGGTGACGCCGCGCGCATCGCCTTCCTGCTGGCCTGCTTTGC
CGAATACCTGCGCGGCCGCGACGACAACGGCGCCGCCTATTCCGTCACCGAACCGCATCTGGGCCCCGACGACCTGCCCC
TGGCCGGCGACGCCGATTCGGCACGGGCGCTGGACATGTCGGTCTTTGCCGGATGGGGGTTGCGCGACCACCCCGATTTC
GTCGCCCTGTTCGTCCGCACGCGCGCCGCGATCCGCGCCGGGGGCACCCGCGCGACCCTACACACACTTGTGACGGAGGC
GGACAAGGCCGCCTGA

Upstream 100 bases:

>100_bases
TCTCAGCGTCGGCAATTTCCACCGCGCGCACCAGGCCGTCTATATCGACCGCGTCCTGGGGCAGCAGGACAATGCGGGAT
GGGGCATCCTGGGCGTCGGC

Downstream 100 bases:

>100_bases
CCGGGGATCGAGCGGGGGCCGATCCCAATATCACCAAAATGTGAATACGAGTTCAGGTCGAGAAAATCGTTCTGCCCGCC
AAAGGGTCCGTTCCGCGTTC

Product: D-mannonate oxidoreductase

Products: D-Fructuronate; H+; Nicotinamide adenine dinucleotide - reduced [C]

Alternate protein names: NA

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MMDDARERAKAESLRAQDGLYTLTECPADAPDTVRVIGSIVEYLHAPDDRAAAIRRLADPAIRIVSLTVTEGGYYVDESG
QFQIDHPTIAEDLARDVPHTVFGLATEALRLRRDSGAGPFTILSCDNLPHNGHVARTAFLSWARARDADLAAWVEAHVTF
PCTMVDRITPAVRDPDIARLDAASGIADRAPVFCEDFIQWVVEDTFCAGRPPLEQVGVLFTGDVAPYERVKLRMLNASHS
MLGLPGVLMGYRIVSDIMHNPDVIELLERYLGLDAEPLLQAPPGMELHEYGALLLRRFRNQAISDQLLRIASDSASKLPV
FVRDTAEGTLARGGDAARIAFLLACFAEYLRGRDDNGAAYSVTEPHLGPDDLPLAGDADSARALDMSVFAGWGLRDHPDF
VALFVRTRAAIRAGGTRATLHTLVTEADKAA

Sequences:

>Translated_431_residues
MMDDARERAKAESLRAQDGLYTLTECPADAPDTVRVIGSIVEYLHAPDDRAAAIRRLADPAIRIVSLTVTEGGYYVDESG
QFQIDHPTIAEDLARDVPHTVFGLATEALRLRRDSGAGPFTILSCDNLPHNGHVARTAFLSWARARDADLAAWVEAHVTF
PCTMVDRITPAVRDPDIARLDAASGIADRAPVFCEDFIQWVVEDTFCAGRPPLEQVGVLFTGDVAPYERVKLRMLNASHS
MLGLPGVLMGYRIVSDIMHNPDVIELLERYLGLDAEPLLQAPPGMELHEYGALLLRRFRNQAISDQLLRIASDSASKLPV
FVRDTAEGTLARGGDAARIAFLLACFAEYLRGRDDNGAAYSVTEPHLGPDDLPLAGDADSARALDMSVFAGWGLRDHPDF
VALFVRTRAAIRAGGTRATLHTLVTEADKAA
>Mature_431_residues
MMDDARERAKAESLRAQDGLYTLTECPADAPDTVRVIGSIVEYLHAPDDRAAAIRRLADPAIRIVSLTVTEGGYYVDESG
QFQIDHPTIAEDLARDVPHTVFGLATEALRLRRDSGAGPFTILSCDNLPHNGHVARTAFLSWARARDADLAAWVEAHVTF
PCTMVDRITPAVRDPDIARLDAASGIADRAPVFCEDFIQWVVEDTFCAGRPPLEQVGVLFTGDVAPYERVKLRMLNASHS
MLGLPGVLMGYRIVSDIMHNPDVIELLERYLGLDAEPLLQAPPGMELHEYGALLLRRFRNQAISDQLLRIASDSASKLPV
FVRDTAEGTLARGGDAARIAFLLACFAEYLRGRDDNGAAYSVTEPHLGPDDLPLAGDADSARALDMSVFAGWGLRDHPDF
VALFVRTRAAIRAGGTRATLHTLVTEADKAA

Specific function: Hexuronate conversion to KDG. [C]

COG id: COG0246

COG function: function code G; Mannitol-1-phosphate/altronate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannitol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI1790779, Length=366, Percent_Identity=41.2568306010929, Blast_Score=272, Evalue=3e-74,
Organism=Escherichia coli, GI1788497, Length=419, Percent_Identity=39.3794749403341, Blast_Score=259, Evalue=2e-70,
Organism=Escherichia coli, GI1787823, Length=418, Percent_Identity=38.0382775119617, Blast_Score=258, Evalue=6e-70,
Organism=Escherichia coli, GI48994885, Length=365, Percent_Identity=26.5753424657534, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1790028, Length=232, Percent_Identity=25.8620689655172, Blast_Score=63, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6324401, Length=423, Percent_Identity=37.3522458628842, Blast_Score=273, Evalue=4e-74,
Organism=Saccharomyces cerevisiae, GI6320765, Length=423, Percent_Identity=37.3522458628842, Blast_Score=273, Evalue=4e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR013328
- InterPro:   IPR000669
- InterPro:   IPR013118
- InterPro:   IPR013131
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01232 Mannitol_dh; PF08125 Mannitol_dh_C [H]

EC number: 1.1.1.57 [C]

Molecular weight: Translated: 46950; Mature: 46950

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00974 MANNITOL_DHGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMDDARERAKAESLRAQDGLYTLTECPADAPDTVRVIGSIVEYLHAPDDRAAAIRRLADP
CCCHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCC
AIRIVSLTVTEGGYYVDESGQFQIDHPTIAEDLARDVPHTVFGLATEALRLRRDSGAGPF
CEEEEEEEEECCCEEECCCCCEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCE
TILSCDNLPHNGHVARTAFLSWARARDADLAAWVEAHVTFPCTMVDRITPAVRDPDIARL
EEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHCCCCCCCCCHHHH
DAASGIADRAPVFCEDFIQWVVEDTFCAGRPPLEQVGVLFTGDVAPYERVKLRMLNASHS
HHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCEEEECCCCCHHHHHEEEECCCCC
MLGLPGVLMGYRIVSDIMHNPDVIELLERYLGLDAEPLLQAPPGMELHEYGALLLRRFRN
HHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHHHHHHH
QAISDQLLRIASDSASKLPVFVRDTAEGTLARGGDAARIAFLLACFAEYLRGRDDNGAAY
HHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEE
SVTEPHLGPDDLPLAGDADSARALDMSVFAGWGLRDHPDFVALFVRTRAAIRAGGTRATL
EECCCCCCCCCCCCCCCCCCCHHEEHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHH
HTLVTEADKAA
HHHHHHHHCCC
>Mature Secondary Structure
MMDDARERAKAESLRAQDGLYTLTECPADAPDTVRVIGSIVEYLHAPDDRAAAIRRLADP
CCCHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCC
AIRIVSLTVTEGGYYVDESGQFQIDHPTIAEDLARDVPHTVFGLATEALRLRRDSGAGPF
CEEEEEEEEECCCEEECCCCCEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCE
TILSCDNLPHNGHVARTAFLSWARARDADLAAWVEAHVTFPCTMVDRITPAVRDPDIARL
EEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHCCCCCCCCCHHHH
DAASGIADRAPVFCEDFIQWVVEDTFCAGRPPLEQVGVLFTGDVAPYERVKLRMLNASHS
HHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCEEEECCCCCHHHHHEEEECCCCC
MLGLPGVLMGYRIVSDIMHNPDVIELLERYLGLDAEPLLQAPPGMELHEYGALLLRRFRN
HHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHHHHHHH
QAISDQLLRIASDSASKLPVFVRDTAEGTLARGGDAARIAFLLACFAEYLRGRDDNGAAY
HHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEE
SVTEPHLGPDDLPLAGDADSARALDMSVFAGWGLRDHPDFVALFVRTRAAIRAGGTRATL
EECCCCCCCCCCCCCCCCCCCHHEEHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHH
HTLVTEADKAA
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+; NADH; NADPH [C]

Metal ions: No metal ion requirement [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 1 {D-fructuronic} 0.2 {NAD+}} 0.03 {NADH}} 0.7 {NADPH}} 1.8 {mannonate}} 1.7 {mannonate}} 0.5 {fructuronate}} [C]

Substrates: D-Mannonate; Nicotinamide adenine dinucleotide [C]

Specific reaction: D-Mannonate + Nicotinamide adenine dinucleotide <==> D-Fructuronate + H+ + Nicotinamide adenine dinucleotide - reduced [C]

General reaction: Redox reaction [C]

Inhibitor: Altronate; ATP; Fructuronate; Mannonate; NAD+; NADH; p-Chloromercuribenzoate; Tagaturonate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA