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The map label for this gene is ygfZ [C]

Identifier: 162149036

GI number: 162149036

Start: 3357446

End: 3358321

Strand: Direct

Name: ygfZ [C]

Synonym: GDI_3266

Alternate gene names: 162149036

Gene position: 3357446-3358321 (Clockwise)

Preceding gene: 162149028

Following gene: 162149037

Centisome position: 85.12

GC content: 70.21

Gene sequence:

>876_bases
GTGGCTTTCCCCTTGTCGCCGGGGGCGCATGCGACCAACATCCATACCGCCATGACACATTTCGCCTTTCTACCGGACCG
TGCCGTGCTGGCCATCTCCGGCGCCGACCGGGTGTCCTTCCTCCAGGGCCTGGTATCGAACGACGTTGCCGCCGTCGCAC
CCGGCCAGGCGGTCTGGACCGCGTTCCTGACGCCGCAGGGGAAATGGCAGGCCGATTTCTTCCTGTTCGCCGAAGCGGAC
GGCGAACGCCTGCTGCTGGATTGCGAGGCCGCGCAGGCCGACATGCTGCGCCAGCGCCTGGCGCGCTACCGCCTGCGCTC
CGACGTGTCGATCGACCCGACCGGCTTTGCCGTCCATGCCGCGTGGGGCGCGGTGCCGCCCATGCTGGATTCGGCCATCG
GCGCCCCCGATCCGCGCCTGGCCGAGGCCGGCTGGCGCCTGATCCTGCCCCGCCCCACCCCCGACGCGGCCGACCACGCG
GCCTATGACGCCCATCGGCTGGCGCTGGGCCTGCCGGACGGATCGCGCGACTGCGAGGAAGGCAAGACCCTGCTTCTGGA
AGCGAATTTCGAGGCCCTGAACGGCATCTCCTGGACCAAGGGCTGCTATATGGGCCAGGAACTGACGGCCCGCACCCGGT
ATCGCGGCCTGGTCAGGCGCAAGCTGCTGCCCGTGTCCGGCGCGGCCCTGCCGCCGCCCGGCACCCCGCTGATGCATGGC
GAGAAGGAGGCCGGAATCATGGCCTCGTCCCGCGACGGGCGGGGGCTGGCGATGCTGCGACTGGACCATCGCAGCGCCGA
GCTGACGGCCGAGGGCCATTCGGTCCAGGTCCACATTCCTTCCTGGCTGACGCTCAAGACCGAAGCCGAAGGCTGA

Upstream 100 bases:

>100_bases
AATCCGCATACCCCGCGACACTAGCCCATTGCCCGGGCGGGTGAAATCGGTCCATGGGGCCGGCGCATGCCCGATATATG
GTCCCCGGAGGGATTCTCCC

Downstream 100 bases:

>100_bases
CCCGGCGTGGCCGGCGACACGCCGAACGACCGCCCAAGGATGACCATGACCTACCTTCCCCCCGACGACGCCACACGGGC
CCGCCTGCTCGACCAGGTCC

Product: aminomethyltransferase protein (glycine cleavage)

Products: protein-dihydrolipoyllysine; 5,10-methylenetetrahydrofolate; NH3

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MAFPLSPGAHATNIHTAMTHFAFLPDRAVLAISGADRVSFLQGLVSNDVAAVAPGQAVWTAFLTPQGKWQADFFLFAEAD
GERLLLDCEAAQADMLRQRLARYRLRSDVSIDPTGFAVHAAWGAVPPMLDSAIGAPDPRLAEAGWRLILPRPTPDAADHA
AYDAHRLALGLPDGSRDCEEGKTLLLEANFEALNGISWTKGCYMGQELTARTRYRGLVRRKLLPVSGAALPPPGTPLMHG
EKEAGIMASSRDGRGLAMLRLDHRSAELTAEGHSVQVHIPSWLTLKTEAEG

Sequences:

>Translated_291_residues
MAFPLSPGAHATNIHTAMTHFAFLPDRAVLAISGADRVSFLQGLVSNDVAAVAPGQAVWTAFLTPQGKWQADFFLFAEAD
GERLLLDCEAAQADMLRQRLARYRLRSDVSIDPTGFAVHAAWGAVPPMLDSAIGAPDPRLAEAGWRLILPRPTPDAADHA
AYDAHRLALGLPDGSRDCEEGKTLLLEANFEALNGISWTKGCYMGQELTARTRYRGLVRRKLLPVSGAALPPPGTPLMHG
EKEAGIMASSRDGRGLAMLRLDHRSAELTAEGHSVQVHIPSWLTLKTEAEG
>Mature_290_residues
AFPLSPGAHATNIHTAMTHFAFLPDRAVLAISGADRVSFLQGLVSNDVAAVAPGQAVWTAFLTPQGKWQADFFLFAEADG
ERLLLDCEAAQADMLRQRLARYRLRSDVSIDPTGFAVHAAWGAVPPMLDSAIGAPDPRLAEAGWRLILPRPTPDAADHAA
YDAHRLALGLPDGSRDCEEGKTLLLEANFEALNGISWTKGCYMGQELTARTRYRGLVRRKLLPVSGAALPPPGTPLMHGE
KEAGIMASSRDGRGLAMLRLDHRSAELTAEGHSVQVHIPSWLTLKTEAEG

Specific function: Folate-binding protein involved in regulating the level of ATP-dnaA and in the modification of some tRNAs. It is probably a key factor in regulatory networks that act via tRNA modification, such as initiation of chromosomal replication [H]

COG id: COG0354

COG function: function code R; Predicted aminomethyltransferase related to GcvT

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA-modifying ygfZ family [H]

Homologues:

Organism=Homo sapiens, GI58197556, Length=300, Percent_Identity=30.3333333333333, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI71985951, Length=263, Percent_Identity=30.4182509505703, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24645123, Length=296, Percent_Identity=29.0540540540541, Blast_Score=120, Evalue=1e-27,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017703
- InterPro:   IPR006222 [H]

Pfam domain/function: PF01571 GCV_T [H]

EC number: 2.1.2.10

Molecular weight: Translated: 31343; Mature: 31212

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFPLSPGAHATNIHTAMTHFAFLPDRAVLAISGADRVSFLQGLVSNDVAAVAPGQAVWT
CCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCEEECCCCEEEE
AFLTPQGKWQADFFLFAEADGERLLLDCEAAQADMLRQRLARYRLRSDVSIDPTGFAVHA
EEECCCCCCCEEEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEE
AWGAVPPMLDSAIGAPDPRLAEAGWRLILPRPTPDAADHAAYDAHRLALGLPDGSRDCEE
HHCCCCHHHHHHCCCCCCCHHHCCCEEEECCCCCCCCHHHHCCCEEEEEECCCCCCCCCC
GKTLLLEANFEALNGISWTKGCYMGQELTARTRYRGLVRRKLLPVSGAALPPPGTPLMHG
CCEEEEEECHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
EKEAGIMASSRDGRGLAMLRLDHRSAELTAEGHSVQVHIPSWLTLKTEAEG
CCCCCEEEECCCCCEEEEEEECCCCCEEEECCCEEEEECCCEEEEEECCCC
>Mature Secondary Structure 
AFPLSPGAHATNIHTAMTHFAFLPDRAVLAISGADRVSFLQGLVSNDVAAVAPGQAVWT
CCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCEEECCCCEEEE
AFLTPQGKWQADFFLFAEADGERLLLDCEAAQADMLRQRLARYRLRSDVSIDPTGFAVHA
EEECCCCCCCEEEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEE
AWGAVPPMLDSAIGAPDPRLAEAGWRLILPRPTPDAADHAAYDAHRLALGLPDGSRDCEE
HHCCCCHHHHHHCCCCCCCHHHCCCEEEECCCCCCCCHHHHCCCEEEEEECCCCCCCCCC
GKTLLLEANFEALNGISWTKGCYMGQELTARTRYRGLVRRKLLPVSGAALPPPGTPLMHG
CCEEEEEECHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
EKEAGIMASSRDGRGLAMLRLDHRSAELTAEGHSVQVHIPSWLTLKTEAEG
CCCCCEEEECCCCCEEEEEEECCCCCEEEECCCEEEEECCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein-S-aminomethyldihydrolipoyllysine; tetrahydrofolate

Specific reaction: protein-S-aminomethyldihydrolipoyllysine + tetrahydrofolate = protein-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH3

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA