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The map label for this gene is sir2 [H]

Identifier: 162149009

GI number: 162149009

Start: 3329204

End: 3330898

Strand: Direct

Name: sir2 [H]

Synonym: GDI_3239

Alternate gene names: 162149009

Gene position: 3329204-3330898 (Clockwise)

Preceding gene: 162149008

Following gene: 162149010

Centisome position: 84.41

GC content: 67.2

Gene sequence:

>1695_bases
ATGCAAGCTGACACCACAGCCCCCACGCTGCCGGTCGGGCATTACGCCTACGACCAGGTGGACCGCGCCTTCCTCGCCGA
CCGGGTGGAGCAGTTCCGCGACCAGGTGGCCCGCCGCCTGTCCGGCGCCCTGTCCGAGGATGAATTCAAGCCGCTACGCC
TGATGAACGGCCTGTATCTGCAACTGCACGCCTACATGCTGCGGATCGCGATCCCATACGGGATGCTGGATTCCCGCCAG
ATGCGCGAACTGGCCCATATCGCGCGGACCTATGACCGCGATTACGGCCATTTCACCACGCGGCAGAACCTGCAGTTCAA
CTGGATCAGGCTGGAAGACACGCCCGATATCCTGGGCCGGCTGGCCGGGGTGGACATGCATGCCATCCAGACCAGCGGCA
ACTGCATCCGCAACGTGACCTGCGACCAGTTCGCCGGTGCCGCCGTGGACGAACTGGTCGACCCGCGCGTGCATGCCGAA
ATCCTGCGCCAGTGGTCGACGCTGCATCCCGAATTCTCGTTCCTGCCGCGCAAGTTCAAGATCGCGATCAGCGGCAGCCC
GAACGACCGCGTGGCCGCCCGCTTCCACGATATCGGGCTGGTGGCCAAGCCGGGGCCGGACGGGCGGACGGTGTTCGACG
TCTTCGTCGGCGGCGGGATGGGCCGGACGCCGATCATCGGCGTGCATCTGCGCGACGACCTGCCCGAGGAAGACCTGCTG
GCCTATCTGGAAGCGATCATCCGCGTCTATAACGAGTACGGCCGCCGCGACAACATCTACAAGGCACGCATCAAGATCCT
GGTCCAGGCGCTGGGGGTCGAGGGCTATCGCGCCCGGGTGGACGCCGAATTCGCCGCCATGGACCGCGCGCTCTATCGCC
TGCCGCCCGAAACGGTGGCCGCCATCCGCGCGCGCTTCGGCACGCCCGACCTGGATGCGCCCGCCGATGCAGCGGGGCAT
CTGGACCGCGCCCGGCGCGCCGATCCGGCCTTCGACCGCTGGGTCCGCACCAACACCCACCCCCACCGCACGGCGGGATA
TATCTCGGCCGTCATCTCGCTGAAGCCCGATGGCGGCATTCCGGGCGACGCGACCTCGGCCCAGATGGACGTCATCGCGG
ACCTGGCCGACCGCTACGCCTTCGGCGAAATCCGCATCACCCACATGCAGAACCTGGTACTGGGCCATGTCCGCCAGGAC
GCGCTTCACGCGCTGTGGCAGGCCCTGCGCGCCGCCGGGCTGGCCACCGCCAATATCGGGCTGATCGGCGACATCATCGC
CTGCCCCGGGCTGGATTACTGCTCGCTGGCCAATGCGCGGTCGATCCCGATCGCGCAGAAGCTCAGCGCCCGCTTCGCCA
ACCCGGAACTGCAGGAAAGCATCGGCGACCTGCAGATCAAGATTTCGGGCTGCATCAATGCCTGCGGCCACCACCATGCC
GGCCATATCGGCATCCTGGGCGTGGACAAGAAGGGCGAGGAGTTCTTCCAGCTCACCCTGGGCGGCTCGGCAGGCAGCGA
CGCCGCGATCGGCGAGATCCTGGGCCCGGCCCTGCCCGAGGACGCGACGGTGGACGCCATCGCCCGGCTGGTGGACGCCT
ATCTGGTCCATCGCACTGGTGAGGAACGCTTCCTCGATACCTATCGGCGGCTGGGTGCCGCGCTGTTCAAGGACGCCGTT
TATGCCACTGCTTGA

Upstream 100 bases:

>100_bases
GAGGCCGCGACCCCCGTCCCGGTCACGGTCCGCGAACGGATCCGCGCCTTCGGCCCGACCGTCCATCCCGATTTCGCCAC
CCCCGCCGGGAGTGCCGCCC

Downstream 100 bases:

>100_bases
AGACGGACGACTCGTCGCGGATCGCTGGACCGCCATCGGCCCCGACGACGCCCTGCCCGCGACCGGCCCCGTGATCGTGC
CCCTGGCGCGGATCGAGGAC

Product: sulfite reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 564; Mature: 564

Protein sequence:

>564_residues
MQADTTAPTLPVGHYAYDQVDRAFLADRVEQFRDQVARRLSGALSEDEFKPLRLMNGLYLQLHAYMLRIAIPYGMLDSRQ
MRELAHIARTYDRDYGHFTTRQNLQFNWIRLEDTPDILGRLAGVDMHAIQTSGNCIRNVTCDQFAGAAVDELVDPRVHAE
ILRQWSTLHPEFSFLPRKFKIAISGSPNDRVAARFHDIGLVAKPGPDGRTVFDVFVGGGMGRTPIIGVHLRDDLPEEDLL
AYLEAIIRVYNEYGRRDNIYKARIKILVQALGVEGYRARVDAEFAAMDRALYRLPPETVAAIRARFGTPDLDAPADAAGH
LDRARRADPAFDRWVRTNTHPHRTAGYISAVISLKPDGGIPGDATSAQMDVIADLADRYAFGEIRITHMQNLVLGHVRQD
ALHALWQALRAAGLATANIGLIGDIIACPGLDYCSLANARSIPIAQKLSARFANPELQESIGDLQIKISGCINACGHHHA
GHIGILGVDKKGEEFFQLTLGGSAGSDAAIGEILGPALPEDATVDAIARLVDAYLVHRTGEERFLDTYRRLGAALFKDAV
YATA

Sequences:

>Translated_564_residues
MQADTTAPTLPVGHYAYDQVDRAFLADRVEQFRDQVARRLSGALSEDEFKPLRLMNGLYLQLHAYMLRIAIPYGMLDSRQ
MRELAHIARTYDRDYGHFTTRQNLQFNWIRLEDTPDILGRLAGVDMHAIQTSGNCIRNVTCDQFAGAAVDELVDPRVHAE
ILRQWSTLHPEFSFLPRKFKIAISGSPNDRVAARFHDIGLVAKPGPDGRTVFDVFVGGGMGRTPIIGVHLRDDLPEEDLL
AYLEAIIRVYNEYGRRDNIYKARIKILVQALGVEGYRARVDAEFAAMDRALYRLPPETVAAIRARFGTPDLDAPADAAGH
LDRARRADPAFDRWVRTNTHPHRTAGYISAVISLKPDGGIPGDATSAQMDVIADLADRYAFGEIRITHMQNLVLGHVRQD
ALHALWQALRAAGLATANIGLIGDIIACPGLDYCSLANARSIPIAQKLSARFANPELQESIGDLQIKISGCINACGHHHA
GHIGILGVDKKGEEFFQLTLGGSAGSDAAIGEILGPALPEDATVDAIARLVDAYLVHRTGEERFLDTYRRLGAALFKDAV
YATA
>Mature_564_residues
MQADTTAPTLPVGHYAYDQVDRAFLADRVEQFRDQVARRLSGALSEDEFKPLRLMNGLYLQLHAYMLRIAIPYGMLDSRQ
MRELAHIARTYDRDYGHFTTRQNLQFNWIRLEDTPDILGRLAGVDMHAIQTSGNCIRNVTCDQFAGAAVDELVDPRVHAE
ILRQWSTLHPEFSFLPRKFKIAISGSPNDRVAARFHDIGLVAKPGPDGRTVFDVFVGGGMGRTPIIGVHLRDDLPEEDLL
AYLEAIIRVYNEYGRRDNIYKARIKILVQALGVEGYRARVDAEFAAMDRALYRLPPETVAAIRARFGTPDLDAPADAAGH
LDRARRADPAFDRWVRTNTHPHRTAGYISAVISLKPDGGIPGDATSAQMDVIADLADRYAFGEIRITHMQNLVLGHVRQD
ALHALWQALRAAGLATANIGLIGDIIACPGLDYCSLANARSIPIAQKLSARFANPELQESIGDLQIKISGCINACGHHHA
GHIGILGVDKKGEEFFQLTLGGSAGSDAAIGEILGPALPEDATVDAIARLVDAYLVHRTGEERFLDTYRRLGAALFKDAV
YATA

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6322597, Length=395, Percent_Identity=25.3164556962025, Blast_Score=94, Evalue=8e-20,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.8.7.1 [H]

Molecular weight: Translated: 62113; Mature: 62113

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQADTTAPTLPVGHYAYDQVDRAFLADRVEQFRDQVARRLSGALSEDEFKPLRLMNGLYL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
QLHAYMLRIAIPYGMLDSRQMRELAHIARTYDRDYGHFTTRQNLQFNWIRLEDTPDILGR
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCHHHHHH
LAGVDMHAIQTSGNCIRNVTCDQFAGAAVDELVDPRVHAEILRQWSTLHPEFSFLPRKFK
HHCCCCEEEECCCCEEECCCHHHHCCHHHHHHHCCHHHHHHHHHHHHCCCCHHCCCCEEE
IAISGSPNDRVAARFHDIGLVAKPGPDGRTVFDVFVGGGMGRTPIIGVHLRDDLPEEDLL
EEECCCCCCCHHHHHHHCCEEECCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCHHHHH
AYLEAIIRVYNEYGRRDNIYKARIKILVQALGVEGYRARVDAEFAAMDRALYRLPPETVA
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHCCHHHHH
AIRARFGTPDLDAPADAAGHLDRARRADPAFDRWVRTNTHPHRTAGYISAVISLKPDGGI
HHHHHCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHEEEEEECCCCCC
PGDATSAQMDVIADLADRYAFGEIRITHMQNLVLGHVRQDALHALWQALRAAGLATANIG
CCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
LIGDIIACPGLDYCSLANARSIPIAQKLSARFANPELQESIGDLQIKISGCINACGHHHA
HHHHHHHCCCCCHHHCCCCCCCCHHHHHHHHCCCCHHHHCCCCEEEEEHHHHHHCCCCCC
GHIGILGVDKKGEEFFQLTLGGSAGSDAAIGEILGPALPEDATVDAIARLVDAYLVHRTG
CEEEEEECCCCCCEEEEEEECCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCC
EERFLDTYRRLGAALFKDAVYATA
HHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQADTTAPTLPVGHYAYDQVDRAFLADRVEQFRDQVARRLSGALSEDEFKPLRLMNGLYL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
QLHAYMLRIAIPYGMLDSRQMRELAHIARTYDRDYGHFTTRQNLQFNWIRLEDTPDILGR
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCHHHHHH
LAGVDMHAIQTSGNCIRNVTCDQFAGAAVDELVDPRVHAEILRQWSTLHPEFSFLPRKFK
HHCCCCEEEECCCCEEECCCHHHHCCHHHHHHHCCHHHHHHHHHHHHCCCCHHCCCCEEE
IAISGSPNDRVAARFHDIGLVAKPGPDGRTVFDVFVGGGMGRTPIIGVHLRDDLPEEDLL
EEECCCCCCCHHHHHHHCCEEECCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCHHHHH
AYLEAIIRVYNEYGRRDNIYKARIKILVQALGVEGYRARVDAEFAAMDRALYRLPPETVA
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHCCHHHHH
AIRARFGTPDLDAPADAAGHLDRARRADPAFDRWVRTNTHPHRTAGYISAVISLKPDGGI
HHHHHCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHEEEEEECCCCCC
PGDATSAQMDVIADLADRYAFGEIRITHMQNLVLGHVRQDALHALWQALRAAGLATANIG
CCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
LIGDIIACPGLDYCSLANARSIPIAQKLSARFANPELQESIGDLQIKISGCINACGHHHA
HHHHHHHCCCCCHHHCCCCCCCCHHHHHHHHCCCCHHHHCCCCEEEEEHHHHHHCCCCCC
GHIGILGVDKKGEEFFQLTLGGSAGSDAAIGEILGPALPEDATVDAIARLVDAYLVHRTG
CEEEEEECCCCCCEEEEEEECCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCC
EERFLDTYRRLGAALFKDAVYATA
HHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA