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The map label for this gene is fabI [H]

Identifier: 162149001

GI number: 162149001

Start: 3322486

End: 3323526

Strand: Reverse

Name: fabI [H]

Synonym: GDI_3231

Alternate gene names: 162149001

Gene position: 3323526-3322486 (Counterclockwise)

Preceding gene: 162149004

Following gene: 162149000

Centisome position: 84.26

GC content: 66.19

Gene sequence:

>1041_bases
ATGGGCCTGCGGGCCGAGGTCGCTTTGCCCAGGGTCGCCGTGCGGGGTCGTCATGCCATCCGTTCCATTCCTGCCTGGCC
CGCTTGCCACGATGCCCGCGAAACAGGGTACCTAGTCATGCTCGGATCTTGTCCCACGCGCCTGCTTGTGCAACCACGGC
GCCAATCCGATCATGCCATATTCCATTCTGAGGATCCTATCATGTCAGACGGCACGCCCAAAGCCCCGCTCACCGGCACC
CTCATGAAGGGCAAGCGCGGGCTTGTGATGGGTGTCGCCAATGACCATTCGATCGCCTGGGGGATCGCCAGCGCGGTTGC
GGCACAGGGCGGGGAACTGGCCTTCACCTATCAGGGCGAGGCGCTGGGCAAGCGGGTCCGCCCGCTGGCCGAACGCGTGG
GATCGTCGCTGGTGCTGCCCTGCGACGTGGGCGACGACACCGCGATCGATGCGACCTTCGCCCGGATCGAGGAAGAGTGG
GGGGGGCTGGACTTCCTGGTCCACGCCATCGGCTGGGCGGACAAGCAGTATCTGCGCGGGCGCTATGTCGATACCCCGCG
CGATGCCTTCCTGACGGCGCTGGACATCTCCTGCTATTCCTTCACCGCCGTGGCGCAGCGGGCGGCGCGGCTGATGAAGG
CGGGCGGATCGATGCTGACCCTGTCCTATCTGGGGGCGGAACGGGTCATGCCGCACTACAACGTCATGGGCGTGGCCAAG
GCGGCGCTGGAGGCCTCGGTGCGCTACATGGCCGCCGACCTGGGCCGCGACGGGATTCGCGTGAACGGCATTTCGGCCGG
ACCGATGAAGACGCTGGCCGCCAACGGGATCGGCGATTTCCGCTACATCCTGAGGTGGAACCAGTACAACTCGCCGCTGG
AACGTAACGTGGCGCTGGACGAGGTCGGGGGCGCGGGGCTGTATATGCTGTCCGACCTGTCGGGCGGCGTGACCGGCGAA
ATCCACCACGTCGATTCCGGCTACCATATCGTGGGCATGAAGAACCCCACGGCGCCCGACATCAGCGTTTCGGGCGACTG
A

Upstream 100 bases:

>100_bases
CCATCGCGTTGGGATCGACCGGTTCCGCGGCCTCGGCGTCGCGCATCCATGCCGCGAACAGGGCGAAGGGGTCCGCGCCC
AGATCGATCAGCGGCCCCAG

Downstream 100 bases:

>100_bases
GAAGGCACCCGGGGCGCCGGGGAAAAGCGGGGAGCGTCGGGTCATGTCGCACAATAGTTTCGGCCATCTGTTTCGCGTCA
CGACCTGGGGCGAGAGCCAC

Product: enoyl-[acyl-carrier-protein] reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase 1 [H]

Number of amino acids: Translated: 346; Mature: 345

Protein sequence:

>346_residues
MGLRAEVALPRVAVRGRHAIRSIPAWPACHDARETGYLVMLGSCPTRLLVQPRRQSDHAIFHSEDPIMSDGTPKAPLTGT
LMKGKRGLVMGVANDHSIAWGIASAVAAQGGELAFTYQGEALGKRVRPLAERVGSSLVLPCDVGDDTAIDATFARIEEEW
GGLDFLVHAIGWADKQYLRGRYVDTPRDAFLTALDISCYSFTAVAQRAARLMKAGGSMLTLSYLGAERVMPHYNVMGVAK
AALEASVRYMAADLGRDGIRVNGISAGPMKTLAANGIGDFRYILRWNQYNSPLERNVALDEVGGAGLYMLSDLSGGVTGE
IHHVDSGYHIVGMKNPTAPDISVSGD

Sequences:

>Translated_346_residues
MGLRAEVALPRVAVRGRHAIRSIPAWPACHDARETGYLVMLGSCPTRLLVQPRRQSDHAIFHSEDPIMSDGTPKAPLTGT
LMKGKRGLVMGVANDHSIAWGIASAVAAQGGELAFTYQGEALGKRVRPLAERVGSSLVLPCDVGDDTAIDATFARIEEEW
GGLDFLVHAIGWADKQYLRGRYVDTPRDAFLTALDISCYSFTAVAQRAARLMKAGGSMLTLSYLGAERVMPHYNVMGVAK
AALEASVRYMAADLGRDGIRVNGISAGPMKTLAANGIGDFRYILRWNQYNSPLERNVALDEVGGAGLYMLSDLSGGVTGE
IHHVDSGYHIVGMKNPTAPDISVSGD
>Mature_345_residues
GLRAEVALPRVAVRGRHAIRSIPAWPACHDARETGYLVMLGSCPTRLLVQPRRQSDHAIFHSEDPIMSDGTPKAPLTGTL
MKGKRGLVMGVANDHSIAWGIASAVAAQGGELAFTYQGEALGKRVRPLAERVGSSLVLPCDVGDDTAIDATFARIEEEWG
GLDFLVHAIGWADKQYLRGRYVDTPRDAFLTALDISCYSFTAVAQRAARLMKAGGSMLTLSYLGAERVMPHYNVMGVAKA
ALEASVRYMAADLGRDGIRVNGISAGPMKTLAANGIGDFRYILRWNQYNSPLERNVALDEVGGAGLYMLSDLSGGVTGEI
HHVDSGYHIVGMKNPTAPDISVSGD

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787545, Length=255, Percent_Identity=49.8039215686275, Blast_Score=253, Evalue=1e-68,
Organism=Escherichia coli, GI1789378, Length=253, Percent_Identity=27.6679841897233, Blast_Score=79, Evalue=4e-16,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 37094; Mature: 36963

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLRAEVALPRVAVRGRHAIRSIPAWPACHDARETGYLVMLGSCPTRLLVQPRRQSDHAI
CCCCCCCCCCHHHHCCCHHHHCCCCCCCCCCCCCCCEEEEECCCCCEEEECCCCCCCCEE
FHSEDPIMSDGTPKAPLTGTLMKGKRGLVMGVANDHSIAWGIASAVAAQGGELAFTYQGE
EECCCCCCCCCCCCCCCEEEEECCCCEEEEEECCCCCHHHHHHHHHHCCCCCEEEEECCH
ALGKRVRPLAERVGSSLVLPCDVGDDTAIDATFARIEEEWGGLDFLVHAIGWADKQYLRG
HHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHCC
RYVDTPRDAFLTALDISCYSFTAVAQRAARLMKAGGSMLTLSYLGAERVMPHYNVMGVAK
CCCCCCHHHEEEEEECCHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHCCCCCCHHHHHH
AALEASVRYMAADLGRDGIRVNGISAGPMKTLAANGIGDFRYILRWNQYNSPLERNVALD
HHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHCCCCCEEEEEEECCCCCCHHHCCCCC
EVGGAGLYMLSDLSGGVTGEIHHVDSGYHIVGMKNPTAPDISVSGD
CCCCCCEEEEECCCCCCCEEEEEECCCEEEEEECCCCCCCEEECCC
>Mature Secondary Structure 
GLRAEVALPRVAVRGRHAIRSIPAWPACHDARETGYLVMLGSCPTRLLVQPRRQSDHAI
CCCCCCCCCHHHHCCCHHHHCCCCCCCCCCCCCCCEEEEECCCCCEEEECCCCCCCCEE
FHSEDPIMSDGTPKAPLTGTLMKGKRGLVMGVANDHSIAWGIASAVAAQGGELAFTYQGE
EECCCCCCCCCCCCCCCEEEEECCCCEEEEEECCCCCHHHHHHHHHHCCCCCEEEEECCH
ALGKRVRPLAERVGSSLVLPCDVGDDTAIDATFARIEEEWGGLDFLVHAIGWADKQYLRG
HHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHCC
RYVDTPRDAFLTALDISCYSFTAVAQRAARLMKAGGSMLTLSYLGAERVMPHYNVMGVAK
CCCCCCHHHEEEEEECCHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHCCCCCCHHHHHH
AALEASVRYMAADLGRDGIRVNGISAGPMKTLAANGIGDFRYILRWNQYNSPLERNVALD
HHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHCCCCCEEEEEEECCCCCCHHHCCCCC
EVGGAGLYMLSDLSGGVTGEIHHVDSGYHIVGMKNPTAPDISVSGD
CCCCCCEEEEECCCCCCCEEEEEECCCEEEEEECCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]