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The map label for this gene is mraW

Identifier: 162148953

GI number: 162148953

Start: 3268424

End: 3269440

Strand: Direct

Name: mraW

Synonym: GDI_3183

Alternate gene names: 162148953

Gene position: 3268424-3269440 (Clockwise)

Preceding gene: 162148952

Following gene: 162148954

Centisome position: 82.87

GC content: 70.6

Gene sequence:

>1017_bases
ATGAGCACCATCCCTACCTCGGGCCACATCCCGGTCATGCTGGCCGAAGTGCTGGAGATGCTGTGCCCGCGCGACGGCGC
GACCTATCTGGACGGCACGTTCGGCGGTGGCGGCTATGCCCGCGCCATCCTGGGGGCCGCGCGCTGCACCCTGTGGGCGA
TCGACCGCGACCCTGCCGCCATCCGGCGCGGCCAGGCGCTGCAGGCCGAATTGCGCGACGCCGAGGGCGGGATGCGCCTG
CACATGCTCCAGGGCGGGTTCGGCGACATGCAGGGCCTGCTGGCCGAAAGCGGGGTGCACAGCCTGGACGGCGTGGTGCT
GGATCTGGGCGTGTCGTCCTTCCAGCTCGACGAGGTCGAACGCGGCTTTTCCTTCCGCACCGACGGCCCGCTGGACATGC
GCATGGGCGATACCGGCCCCACGGCGGCCGACATCGTGAATACGATGGGCGAATCCGACCTGGCCGACATCATCTATGAA
TATGGCGAGGAACGGATGTCCCGCCGGGTCGCGCGCGCCATCGTCGCCGCCCGCGCGCAGGCGCCGATCACGACCACATT
TCAACTGGCCGACATCATCCGCTCGGTCGTGCCGGGCGACCGTTCGGGCATCGACCCGGCCACGCGTTCGTTCCAAGGCA
TCCGCATCCACGTCAATGACGAGCTGGGGCAGATCGTAACGGGACTGGAACAGGCGCTGGCGCTGCTGGCCCCCGGCGGC
CGGCTGGTCGTGGTGTCGTTCCACTCATTGGAAGACCGGCTGGTCAAGCAGGCGCTGAACCGCGCCGCCGGCCGCATGCC
CGGCCCCTCGCGCCACGATCCCCGCTCCATGCGGGCCGAGCAGGCGGCGCCGGACTTCCGGCTGCTGACCAGCCGCGCCC
TGCGCCCCGGCGAGACGGAATGCCGCGCCAACCCCCGCGCCCGCAGCGCGCGTCTGCGCGCGGCGGAGCGCCTTGCGGTC
CCGGACACCCAGAACAGCACCCATAATAATACCAGCCCCCAGGAACGCCGCCCATGA

Upstream 100 bases:

>100_bases
GGGAACCCGCCGCCGCCGAGCGCCGCAGGGCGGAGGCCCGCACCCGCTCGCGCCAGCTCGCCCTGCCCGCCCAGGGCCGC
CGCCAGGGGGGCGCGGACGC

Downstream 100 bases:

>100_bases
TGCGATCCTTCACAATCCTCTGCGCCATGATGGCCGGCCTGTCCGGGCTGTACCTGTATTCCACGAAGCACCAGACCACG
CTTCTGGACCAGCAGATCTC

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MSTIPTSGHIPVMLAEVLEMLCPRDGATYLDGTFGGGGYARAILGAARCTLWAIDRDPAAIRRGQALQAELRDAEGGMRL
HMLQGGFGDMQGLLAESGVHSLDGVVLDLGVSSFQLDEVERGFSFRTDGPLDMRMGDTGPTAADIVNTMGESDLADIIYE
YGEERMSRRVARAIVAARAQAPITTTFQLADIIRSVVPGDRSGIDPATRSFQGIRIHVNDELGQIVTGLEQALALLAPGG
RLVVVSFHSLEDRLVKQALNRAAGRMPGPSRHDPRSMRAEQAAPDFRLLTSRALRPGETECRANPRARSARLRAAERLAV
PDTQNSTHNNTSPQERRP

Sequences:

>Translated_338_residues
MSTIPTSGHIPVMLAEVLEMLCPRDGATYLDGTFGGGGYARAILGAARCTLWAIDRDPAAIRRGQALQAELRDAEGGMRL
HMLQGGFGDMQGLLAESGVHSLDGVVLDLGVSSFQLDEVERGFSFRTDGPLDMRMGDTGPTAADIVNTMGESDLADIIYE
YGEERMSRRVARAIVAARAQAPITTTFQLADIIRSVVPGDRSGIDPATRSFQGIRIHVNDELGQIVTGLEQALALLAPGG
RLVVVSFHSLEDRLVKQALNRAAGRMPGPSRHDPRSMRAEQAAPDFRLLTSRALRPGETECRANPRARSARLRAAERLAV
PDTQNSTHNNTSPQERRP
>Mature_337_residues
STIPTSGHIPVMLAEVLEMLCPRDGATYLDGTFGGGGYARAILGAARCTLWAIDRDPAAIRRGQALQAELRDAEGGMRLH
MLQGGFGDMQGLLAESGVHSLDGVVLDLGVSSFQLDEVERGFSFRTDGPLDMRMGDTGPTAADIVNTMGESDLADIIYEY
GEERMSRRVARAIVAARAQAPITTTFQLADIIRSVVPGDRSGIDPATRSFQGIRIHVNDELGQIVTGLEQALALLAPGGR
LVVVSFHSLEDRLVKQALNRAAGRMPGPSRHDPRSMRAEQAAPDFRLLTSRALRPGETECRANPRARSARLRAAERLAVP
DTQNSTHNNTSPQERRP

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=342, Percent_Identity=40.9356725146199, Blast_Score=228, Evalue=4e-60,
Organism=Homo sapiens, GI165377202, Length=193, Percent_Identity=47.1502590673575, Blast_Score=157, Evalue=2e-38,
Organism=Escherichia coli, GI1786270, Length=319, Percent_Identity=44.5141065830721, Blast_Score=220, Evalue=9e-59,
Organism=Drosophila melanogaster, GI62472493, Length=334, Percent_Identity=39.8203592814371, Blast_Score=199, Evalue=2e-51,
Organism=Drosophila melanogaster, GI28571637, Length=334, Percent_Identity=39.8203592814371, Blast_Score=199, Evalue=2e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_GLUDA (A9H0G9)

Other databases:

- EMBL:   CP001189
- EMBL:   AM889285
- RefSeq:   YP_001603414.1
- RefSeq:   YP_002277523.1
- ProteinModelPortal:   A9H0G9
- SMR:   A9H0G9
- GeneID:   5790600
- GeneID:   6976616
- GenomeReviews:   AM889285_GR
- GenomeReviews:   CP001189_GR
- KEGG:   gdj:Gdia_3178
- HOGENOM:   HBG302779
- OMA:   RPINSTL
- ProtClustDB:   PRK00050
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 36501; Mature: 36370

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: BINDING 55-55 BINDING 87-87 BINDING 108-108 BINDING 115-115

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTIPTSGHIPVMLAEVLEMLCPRDGATYLDGTFGGGGYARAILGAARCTLWAIDRDPAA
CCCCCCCCCHHHHHHHHHHHHCCCCCCCEECCCCCCCHHHHHHHHHHHHEEEEECCCHHH
IRRGQALQAELRDAEGGMRLHMLQGGFGDMQGLLAESGVHSLDGVVLDLGVSSFQLDEVE
HHCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCHHHCCEEEECCCCHHHHHHHH
RGFSFRTDGPLDMRMGDTGPTAADIVNTMGESDLADIIYEYGEERMSRRVARAIVAARAQ
CCCCCCCCCCCEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
APITTTFQLADIIRSVVPGDRSGIDPATRSFQGIRIHVNDELGQIVTGLEQALALLAPGG
CCCCHHHHHHHHHHHHCCCCCCCCCHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHCCCC
RLVVVSFHSLEDRLVKQALNRAAGRMPGPSRHDPRSMRAEQAAPDFRLLTSRALRPGETE
CEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCHHHHHHHCCCCCCHH
CRANPRARSARLRAAERLAVPDTQNSTHNNTSPQERRP
HCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
STIPTSGHIPVMLAEVLEMLCPRDGATYLDGTFGGGGYARAILGAARCTLWAIDRDPAA
CCCCCCCCHHHHHHHHHHHHCCCCCCCEECCCCCCCHHHHHHHHHHHHEEEEECCCHHH
IRRGQALQAELRDAEGGMRLHMLQGGFGDMQGLLAESGVHSLDGVVLDLGVSSFQLDEVE
HHCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCHHHCCEEEECCCCHHHHHHHH
RGFSFRTDGPLDMRMGDTGPTAADIVNTMGESDLADIIYEYGEERMSRRVARAIVAARAQ
CCCCCCCCCCCEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
APITTTFQLADIIRSVVPGDRSGIDPATRSFQGIRIHVNDELGQIVTGLEQALALLAPGG
CCCCHHHHHHHHHHHHCCCCCCCCCHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHCCCC
RLVVVSFHSLEDRLVKQALNRAAGRMPGPSRHDPRSMRAEQAAPDFRLLTSRALRPGETE
CEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCHHHHHHHCCCCCCHH
CRANPRARSARLRAAERLAVPDTQNSTHNNTSPQERRP
HCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA