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The map label for this gene is dkgB [H]

Identifier: 162148940

GI number: 162148940

Start: 3257981

End: 3258760

Strand: Reverse

Name: dkgB [H]

Synonym: GDI_3170

Alternate gene names: 162148940

Gene position: 3258760-3257981 (Counterclockwise)

Preceding gene: 162148945

Following gene: 162148938

Centisome position: 82.62

GC content: 65.38

Gene sequence:

>780_bases
GTGGCCAGTTTAGCAACAATCGTTCTTGCCGGCTTCGATCCCATGACGCCGGCGGCCTCAATCGCCCGCCACGTTGGCAT
GAATGCGCCGGAACGTTCCGGCCCGCGTTTGCCCCTCCTCGACAGCGAAACCCGCCAAAATCTCCGCGTCGTCCCGCCGC
AGCCGCTCGGGATCGGGCCGCAGCCATTCCGTTCTTCGGGGAGGCAATGCACGACGCAGGGGGCGTGTCATGGCAACCCG
CCGGATTACGACCTCTATTACATCCACTGGCCCAATCCGTCGCAGGATCGCTATGTCGAGGCATGGCGCGCCCTGATCGA
GGCGAAGAAGCGCGGGATGATCCGCTCGATCGCGGTCTGCAACTTCCTGCCGGACTACATCCAGCGCCTGATCGACGAAA
CCGGCGTCGCGCCCAGCATCAACCAGGTCGAGCTGTCGCCCTATTTCCCGCAGGACGACCTGCGCGCGTGGCACGCCGCG
CATGGCATCGCCACCCAGGCCTGGAGCCCGCTGGGACGCGCCAACAGGCTGATTTCCGACCCGCTGCTGGGAAAAATCGC
CGCTCGCCTGGACAGGTGGATCCCGCAAGTGGTGCTGCGCTGGCATCATCAGGTCGGTGCGGTCCCGATCCCCAAGTTGG
CGTCGCGCGCGCGGCAGATCGAGAACATGTCGATCTTTGATTTCGCGCTGACCGACGAAGACATGGCATCCATCGCGACC
CTGGCCCGTCCCGACGGGCGGACCTTCGACCAGGACCCGGCCCGGCACGAGGAATTCTGA

Upstream 100 bases:

>100_bases
CGGAGCGGTCGATCGGGGCGGTCTCGAAAACCGTTGTGCGTGCAAGCGTACCGTGGGTTCGAATCCCACCCTCTCCGCCA
CGTTGAAACGCCTTTGGCGT

Downstream 100 bases:

>100_bases
CCCCGCAAGGGACTCGGATCTGCGGTCATTTCGGAAACGTCGACGTTGTGTTAATTGGTCGGAACCACCTGCAGTCGCAT
TTCAGCCCATGACATAACTC

Product: 2,5-diketo-D-gluconic acid reductase

Products: NA

Alternate protein names: 2,5-DKG reductase B; 2,5-DKGR B; 25DKGR-B; AKR5D [H]

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MASLATIVLAGFDPMTPAASIARHVGMNAPERSGPRLPLLDSETRQNLRVVPPQPLGIGPQPFRSSGRQCTTQGACHGNP
PDYDLYYIHWPNPSQDRYVEAWRALIEAKKRGMIRSIAVCNFLPDYIQRLIDETGVAPSINQVELSPYFPQDDLRAWHAA
HGIATQAWSPLGRANRLISDPLLGKIAARLDRWIPQVVLRWHHQVGAVPIPKLASRARQIENMSIFDFALTDEDMASIAT
LARPDGRTFDQDPARHEEF

Sequences:

>Translated_259_residues
MASLATIVLAGFDPMTPAASIARHVGMNAPERSGPRLPLLDSETRQNLRVVPPQPLGIGPQPFRSSGRQCTTQGACHGNP
PDYDLYYIHWPNPSQDRYVEAWRALIEAKKRGMIRSIAVCNFLPDYIQRLIDETGVAPSINQVELSPYFPQDDLRAWHAA
HGIATQAWSPLGRANRLISDPLLGKIAARLDRWIPQVVLRWHHQVGAVPIPKLASRARQIENMSIFDFALTDEDMASIAT
LARPDGRTFDQDPARHEEF
>Mature_258_residues
ASLATIVLAGFDPMTPAASIARHVGMNAPERSGPRLPLLDSETRQNLRVVPPQPLGIGPQPFRSSGRQCTTQGACHGNPP
DYDLYYIHWPNPSQDRYVEAWRALIEAKKRGMIRSIAVCNFLPDYIQRLIDETGVAPSINQVELSPYFPQDDLRAWHAAH
GIATQAWSPLGRANRLISDPLLGKIAARLDRWIPQVVLRWHHQVGAVPIPKLASRARQIENMSIFDFALTDEDMASIATL
ARPDGRTFDQDPARHEEF

Specific function: Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG). 25DKGR-B has higher catalytic efficiency than 25DKGR-A [H]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI291291012, Length=204, Percent_Identity=33.8235294117647, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI223468663, Length=189, Percent_Identity=33.8624338624339, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI24497577, Length=187, Percent_Identity=29.4117647058824, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI5174391, Length=187, Percent_Identity=29.4117647058824, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI4502049, Length=184, Percent_Identity=30.4347826086957, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI5174695, Length=155, Percent_Identity=34.8387096774194, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI93277124, Length=196, Percent_Identity=28.0612244897959, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI310109922, Length=175, Percent_Identity=31.4285714285714, Blast_Score=81, Evalue=8e-16,
Organism=Homo sapiens, GI310109920, Length=193, Percent_Identity=29.5336787564767, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI45446745, Length=193, Percent_Identity=29.5336787564767, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI4503285, Length=193, Percent_Identity=29.5336787564767, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI5453543, Length=193, Percent_Identity=29.5336787564767, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI24497583, Length=193, Percent_Identity=30.5699481865285, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI300116271, Length=112, Percent_Identity=37.5, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI300116273, Length=137, Percent_Identity=32.1167883211679, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI310109924, Length=149, Percent_Identity=30.8724832214765, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI24497585, Length=155, Percent_Identity=29.6774193548387, Blast_Score=72, Evalue=3e-13,
Organism=Escherichia coli, GI87082198, Length=174, Percent_Identity=44.8275862068966, Blast_Score=157, Evalue=9e-40,
Organism=Escherichia coli, GI1786400, Length=163, Percent_Identity=34.9693251533742, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17564128, Length=190, Percent_Identity=31.0526315789474, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71998625, Length=185, Percent_Identity=30.2702702702703, Blast_Score=98, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17537077, Length=188, Percent_Identity=32.4468085106383, Blast_Score=98, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17537075, Length=188, Percent_Identity=32.9787234042553, Blast_Score=97, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17561298, Length=174, Percent_Identity=32.7586206896552, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17552492, Length=178, Percent_Identity=29.7752808988764, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17538386, Length=168, Percent_Identity=30.3571428571429, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17537079, Length=191, Percent_Identity=27.7486910994764, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17550248, Length=196, Percent_Identity=29.0816326530612, Blast_Score=74, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17561300, Length=169, Percent_Identity=28.4023668639053, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322556, Length=154, Percent_Identity=33.1168831168831, Blast_Score=97, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6324694, Length=196, Percent_Identity=29.0816326530612, Blast_Score=86, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6320576, Length=195, Percent_Identity=28.7179487179487, Blast_Score=84, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6320079, Length=175, Percent_Identity=28.5714285714286, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6321896, Length=204, Percent_Identity=28.921568627451, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319625, Length=100, Percent_Identity=36, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24657054, Length=182, Percent_Identity=34.0659340659341, Blast_Score=98, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24663317, Length=147, Percent_Identity=34.0136054421769, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI45553081, Length=168, Percent_Identity=32.7380952380952, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI281366140, Length=168, Percent_Identity=32.7380952380952, Blast_Score=94, Evalue=7e-20,
Organism=Drosophila melanogaster, GI21356425, Length=179, Percent_Identity=31.2849162011173, Blast_Score=91, Evalue=9e-19,
Organism=Drosophila melanogaster, GI20129731, Length=147, Percent_Identity=32.6530612244898, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24644950, Length=217, Percent_Identity=29.9539170506912, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI221378297, Length=176, Percent_Identity=31.8181818181818, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24662789, Length=188, Percent_Identity=28.1914893617021, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24662785, Length=143, Percent_Identity=30.7692307692308, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24662781, Length=143, Percent_Identity=30.7692307692308, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: =1.1.1.274 [H]

Molecular weight: Translated: 28923; Mature: 28792

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASLATIVLAGFDPMTPAASIARHVGMNAPERSGPRLPLLDSETRQNLRVVPPQPLGIGP
CCCHHEEHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCCEECCCCCCCCCC
QPFRSSGRQCTTQGACHGNPPDYDLYYIHWPNPSQDRYVEAWRALIEAKKRGMIRSIAVC
CHHHHCCCEECCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCHHHHHHHH
NFLPDYIQRLIDETGVAPSINQVELSPYFPQDDLRAWHAAHGIATQAWSPLGRANRLISD
HHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHC
PLLGKIAARLDRWIPQVVLRWHHQVGAVPIPKLASRARQIENMSIFDFALTDEDMASIAT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEEEECCHHHHHHHH
LARPDGRTFDQDPARHEEF
HHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ASLATIVLAGFDPMTPAASIARHVGMNAPERSGPRLPLLDSETRQNLRVVPPQPLGIGP
CCHHEEHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCCEECCCCCCCCCC
QPFRSSGRQCTTQGACHGNPPDYDLYYIHWPNPSQDRYVEAWRALIEAKKRGMIRSIAVC
CHHHHCCCEECCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCHHHHHHHH
NFLPDYIQRLIDETGVAPSINQVELSPYFPQDDLRAWHAAHGIATQAWSPLGRANRLISD
HHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHC
PLLGKIAARLDRWIPQVVLRWHHQVGAVPIPKLASRARQIENMSIFDFALTDEDMASIAT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEEEECCHHHHHHHH
LARPDGRTFDQDPARHEEF
HHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA