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The map label for this gene is mtfA [H]

Identifier: 162148880

GI number: 162148880

Start: 3203855

End: 3204862

Strand: Reverse

Name: mtfA [H]

Synonym: GDI_3110

Alternate gene names: 162148880

Gene position: 3204862-3203855 (Counterclockwise)

Preceding gene: 162148881

Following gene: 162148879

Centisome position: 81.26

GC content: 70.83

Gene sequence:

>1008_bases
ATGACTGAAGACGTGGCCGGCGTGCCGGTGGAGCTGCCGCATGCCGTGGGCGCGGCCTATCTGGCCGCCGAGGGGTTCGA
GGACGCCCTGTCCGAGGAACTGCGGCGCAAGGGCGTGGGCATCATGGCCTGGCACGGGCGGCTGGCGCTGTCCGCCGAAC
CGCCGGTCGCGGCGGCCTGGTCGCTGGACACCTGGCTCGAGCCCGAACGCCATCCCGTGGCCTCGATCGGTGCCGCGGCG
GCGTTGCTGCGCGCGCGGCAGCGCAACTGGGCGTGCCATGCCGTGCGGCATCACCGCCGGGCGACGCTGATCACCGACCG
GCTGCCTCCGGTCGCCGCCCGACCGCTGACCTTCCCGGCCGCCGCGCCGGGCGGGCATCTGGGGGCATGGACGCTGCTGG
CGGCGGACAGCATGCTGCTGTCCACGCGCAAATCCTCGCCCTTCGTGAACGGCATCGTCCAGTTCGTCGAGGACCGGGTC
GGGCCGCCGTCGCGGGCCTACCTGAAATTATGGGAGGCCTGTGCCCGCCTGGGCGTCTGGCCAGTACCGGGCGAGCGCTG
CGTCGACCTGGGGGCATCGCCGGGCGGATGGACGTGGGCGATCGCGCAATGCGGGGCGGACGTGGTGGCCGTGGACCGCG
CCGACCTGGACCCGGCCGTGGCCGCGATGCCAGGAGTGACGCTGCGCCGGGAGAGCGCGTTTGGGATCGACCCCGCCGAT
ATCGGCCCGGTGGATTGGCTGTTTTCGGACATCATCGCCTATCCGCCGCGCCTGCTGGCGCTGGCCCGGCGATGGATCGC
GGAGGGCGCCGCGCGCCGGATCGTGATGACCATCAAGTTCCAGGGCGAAACCGATCACGAGACCGCCGAGGCCTTCGCCG
CCATCCCGGGCGGCCGCGTGCTGCACCTGTGGCACAACAAGCATGAACTGACCTTCTTCTGGAATCGCGAGGCGGTCGAG
GCCTATGTGTTTGGTCCCGGGATTTGGTGGTGCATTATTTTCACATGA

Upstream 100 bases:

>100_bases
GGCCTGCATCCCGGCCCGGTGGCGCCGACAGGGGGCAGCCTGCTGATCCTGGCGTGGCTGCTGCTGGCCGTGGATGGAAT
GCGGCGTTGAGGGCGGACGA

Downstream 100 bases:

>100_bases
GTGGAAGGATGCGCTATGGGCCAGGTTCACCACGGAAGCGCCACGACGACAGCGGCAGTCCGTCGAGCGATACAACATAG
TCAAGAGAGCCTGAGGGTTC

Product: RNA 2'-O-ribose methyltransferase mtfA

Products: NA

Alternate protein names: 23S rRNA 2'-O-ribose methyltransferase rlmM [H]

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MTEDVAGVPVELPHAVGAAYLAAEGFEDALSEELRRKGVGIMAWHGRLALSAEPPVAAAWSLDTWLEPERHPVASIGAAA
ALLRARQRNWACHAVRHHRRATLITDRLPPVAARPLTFPAAAPGGHLGAWTLLAADSMLLSTRKSSPFVNGIVQFVEDRV
GPPSRAYLKLWEACARLGVWPVPGERCVDLGASPGGWTWAIAQCGADVVAVDRADLDPAVAAMPGVTLRRESAFGIDPAD
IGPVDWLFSDIIAYPPRLLALARRWIAEGAARRIVMTIKFQGETDHETAEAFAAIPGGRVLHLWHNKHELTFFWNREAVE
AYVFGPGIWWCIIFT

Sequences:

>Translated_335_residues
MTEDVAGVPVELPHAVGAAYLAAEGFEDALSEELRRKGVGIMAWHGRLALSAEPPVAAAWSLDTWLEPERHPVASIGAAA
ALLRARQRNWACHAVRHHRRATLITDRLPPVAARPLTFPAAAPGGHLGAWTLLAADSMLLSTRKSSPFVNGIVQFVEDRV
GPPSRAYLKLWEACARLGVWPVPGERCVDLGASPGGWTWAIAQCGADVVAVDRADLDPAVAAMPGVTLRRESAFGIDPAD
IGPVDWLFSDIIAYPPRLLALARRWIAEGAARRIVMTIKFQGETDHETAEAFAAIPGGRVLHLWHNKHELTFFWNREAVE
AYVFGPGIWWCIIFT
>Mature_334_residues
TEDVAGVPVELPHAVGAAYLAAEGFEDALSEELRRKGVGIMAWHGRLALSAEPPVAAAWSLDTWLEPERHPVASIGAAAA
LLRARQRNWACHAVRHHRRATLITDRLPPVAARPLTFPAAAPGGHLGAWTLLAADSMLLSTRKSSPFVNGIVQFVEDRVG
PPSRAYLKLWEACARLGVWPVPGERCVDLGASPGGWTWAIAQCGADVVAVDRADLDPAVAAMPGVTLRRESAFGIDPADI
GPVDWLFSDIIAYPPRLLALARRWIAEGAARRIVMTIKFQGETDHETAEAFAAIPGGRVLHLWHNKHELTFFWNREAVEA
YVFGPGIWWCIIFT

Specific function: Catalyzes the 2'-O-methylation at nucleotide C2498 in 23S rRNA [H]

COG id: COG2933

COG function: function code R; Predicted SAM-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family. RlmM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789171, Length=195, Percent_Identity=28.2051282051282, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011224
- InterPro:   IPR002877 [H]

Pfam domain/function: PF01728 FtsJ [H]

EC number: NA

Molecular weight: Translated: 36466; Mature: 36335

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEDVAGVPVELPHAVGAAYLAAEGFEDALSEELRRKGVGIMAWHGRLALSAEPPVAAAW
CCCCCCCCCEECCHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEECCEEEECCCCCCEEEE
SLDTWLEPERHPVASIGAAAALLRARQRNWACHAVRHHRRATLITDRLPPVAARPLTFPA
CCHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCHHEEEECCCCCCCCCCCCCCC
AAPGGHLGAWTLLAADSMLLSTRKSSPFVNGIVQFVEDRVGPPSRAYLKLWEACARLGVW
CCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
PVPGERCVDLGASPGGWTWAIAQCGADVVAVDRADLDPAVAAMPGVTLRRESAFGIDPAD
CCCCHHHHCCCCCCCCCEEEHHHCCCCEEEEECCCCCHHHHHCCCCEEECCCCCCCCCCC
IGPVDWLFSDIIAYPPRLLALARRWIAEGAARRIVMTIKFQGETDHETAEAFAAIPGGRV
CCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHCCCCCCE
LHLWHNKHELTFFWNREAVEAYVFGPGIWWCIIFT
EEEEECCEEEEEEECCCEEEEEEECCCEEEEEEEC
>Mature Secondary Structure 
TEDVAGVPVELPHAVGAAYLAAEGFEDALSEELRRKGVGIMAWHGRLALSAEPPVAAAW
CCCCCCCCEECCHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEECCEEEECCCCCCEEEE
SLDTWLEPERHPVASIGAAAALLRARQRNWACHAVRHHRRATLITDRLPPVAARPLTFPA
CCHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCHHEEEECCCCCCCCCCCCCCC
AAPGGHLGAWTLLAADSMLLSTRKSSPFVNGIVQFVEDRVGPPSRAYLKLWEACARLGVW
CCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
PVPGERCVDLGASPGGWTWAIAQCGADVVAVDRADLDPAVAAMPGVTLRRESAFGIDPAD
CCCCHHHHCCCCCCCCCEEEHHHCCCCEEEEECCCCCHHHHHCCCCEEECCCCCCCCCCC
IGPVDWLFSDIIAYPPRLLALARRWIAEGAARRIVMTIKFQGETDHETAEAFAAIPGGRV
CCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHCCCCCCE
LHLWHNKHELTFFWNREAVEAYVFGPGIWWCIIFT
EEEEECCEEEEEEECCCEEEEEEECCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA