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The map label for this gene is hup [H]

Identifier: 162148812

GI number: 162148812

Start: 3132183

End: 3132467

Strand: Reverse

Name: hup [H]

Synonym: GDI_3041

Alternate gene names: 162148812

Gene position: 3132467-3132183 (Counterclockwise)

Preceding gene: 162148813

Following gene: 162148811

Centisome position: 79.42

GC content: 62.11

Gene sequence:

>285_bases
ATGGAGAAGCCACTCAACAAGCAGGAACTCGTCGCCGCCGTGGCCGACGACGCTGATCTGCCGAAGGCAAAGGCCGGAGA
AGTGGTCGATGCCGTGTTCAGCGCTATCGAGAAGGCCCTGTCGAAGAAGCAGGAAGTGCGTCTGGTGGGTTTCGGCACGT
TCGTGACGGCGACGCGCAAGGCAGCCAAGGGTCGCAACCCCCGTACGGGCGAGGAAATCGACATTCCGGCGTCCACCTCG
GTTCGTTTCAAGCCGGGCAAGAACCTGAAGGAGGCCGTGAGCTGA

Upstream 100 bases:

>100_bases
GGTCGGTTGACGCCGCGAAAAACGGCTGCATAGTGCGGCCCAATGCGGCGTAGGCAATCATATCGGTCCTGGCGCGCGCG
CAGTGAAAGAAAGGCGTAAA

Downstream 100 bases:

>100_bases
TCACGCGCTGACTTTGGCAGGGGCCTGGCCGGCCGCGCGTCGTGCCGTGGCCCCTTTTATTTTTTTCCGTCTTTGGGGCG
ATTAGCTCAGCGGTAGAGCG

Product: DNA-binding protein HU

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 94; Mature: 94

Protein sequence:

>94_residues
MEKPLNKQELVAAVADDADLPKAKAGEVVDAVFSAIEKALSKKQEVRLVGFGTFVTATRKAAKGRNPRTGEEIDIPASTS
VRFKPGKNLKEAVS

Sequences:

>Translated_94_residues
MEKPLNKQELVAAVADDADLPKAKAGEVVDAVFSAIEKALSKKQEVRLVGFGTFVTATRKAAKGRNPRTGEEIDIPASTS
VRFKPGKNLKEAVS
>Mature_94_residues
MEKPLNKQELVAAVADDADLPKAKAGEVVDAVFSAIEKALSKKQEVRLVGFGTFVTATRKAAKGRNPRTGEEIDIPASTS
VRFKPGKNLKEAVS

Specific function: Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions [H]

COG id: COG0776

COG function: function code L; Bacterial nucleoid DNA-binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial histone-like protein family [H]

Homologues:

Organism=Escherichia coli, GI1786644, Length=90, Percent_Identity=44.4444444444444, Blast_Score=80, Evalue=3e-17,
Organism=Escherichia coli, GI1790433, Length=89, Percent_Identity=41.5730337078652, Blast_Score=70, Evalue=4e-14,
Organism=Escherichia coli, GI1788005, Length=89, Percent_Identity=40.4494382022472, Blast_Score=67, Evalue=3e-13,

Paralogues:

None

Copy number: 860 Molecules/Cell In: Growth-Phase, Minimal Media (Based on E. coli). 2040 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000119
- InterPro:   IPR020816
- InterPro:   IPR010992 [H]

Pfam domain/function: PF00216 Bac_DNA_binding [H]

EC number: NA

Molecular weight: Translated: 10038; Mature: 10038

Theoretical pI: Translated: 10.32; Mature: 10.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKPLNKQELVAAVADDADLPKAKAGEVVDAVFSAIEKALSKKQEVRLVGFGTFVTATRK
CCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCEEEECCHHHHHHHHH
AAKGRNPRTGEEIDIPASTSVRFKPGKNLKEAVS
HHCCCCCCCCCEECCCCCCCEEECCCCCHHHHCC
>Mature Secondary Structure
MEKPLNKQELVAAVADDADLPKAKAGEVVDAVFSAIEKALSKKQEVRLVGFGTFVTATRK
CCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCEEEECCHHHHHHHHH
AAKGRNPRTGEEIDIPASTSVRFKPGKNLKEAVS
HHCCCCCCCCCEECCCCCCCEEECCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA