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The map label for this gene is nuoB

Identifier: 162148810

GI number: 162148810

Start: 3130619

End: 3131194

Strand: Reverse

Name: nuoB

Synonym: GDI_3039

Alternate gene names: 162148810

Gene position: 3131194-3130619 (Counterclockwise)

Preceding gene: 162148811

Following gene: 162148809

Centisome position: 79.39

GC content: 65.62

Gene sequence:

>576_bases
ATGAGTGCCGCGAACGCGACGGGCGAGGGCCAGGACGCCATCGCCTGGAACAGGGAGATGCTGCCTCCGGGGCCGGAGCA
GGATGCCGTCATCAAGGGCATCACCGGCGAAATCACCGAGAAGGGCTTCGTCGTCGCCAGCCTGGACAAGCTGGTGAACT
GGGGCCGAACCGGCAGCCTGTGGCCGATGTCCTTCGGTCTGGCCTGCTGCGCCGTTGAAATGATCCACGCCTACATGCCG
CGCTACGACCTGGACCGGATGGGCATCATCCCCCGCGGGTCGCCGCGCCAGTCGGACGTGATGATCGTGGCGGGCACCCT
GACCAACAAGATGGCGCCGGCCCTGCGCCGGGTCTATGACCAGATGGCCGAGCCCCGCTGGGTCATCTCGATGGGCTCCT
GCGCCAATGGCGGGGGATACTACCATTATTCCTATTCCGTGGTGCGCGGCTGCGACCGGATCGTGCCGGTGGACGTCTAC
GTCCCCGGCTGCCCGCCGACGGCCGAGGCCCTGATCTACGGCATCTTTCAACTGCAGAAGAAAATTCGCAGGACAGGGAC
GATTCTCCGTGGCTGA

Upstream 100 bases:

>100_bases
TCGATGATGGGTTTCCTGGGCATCCTGACGGTCGGGTTCATTTACGAATGGTGCAAGGGCGCGCTGGACTGGGATTGATG
TACGGCAAGGAGACACGGGC

Downstream 100 bases:

>100_bases
CACCGGAACGGATCTGATGGCCCGGACGACCCGGGCATTCGGGGCCCTGGGGTCCCTGGCGTTCGCACTCTCCACCTCGG
TGCCGGGCATCCAGTCCGCG

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B 2; NDH-1 subunit B 2

Number of amino acids: Translated: 191; Mature: 190

Protein sequence:

>191_residues
MSAANATGEGQDAIAWNREMLPPGPEQDAVIKGITGEITEKGFVVASLDKLVNWGRTGSLWPMSFGLACCAVEMIHAYMP
RYDLDRMGIIPRGSPRQSDVMIVAGTLTNKMAPALRRVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDVY
VPGCPPTAEALIYGIFQLQKKIRRTGTILRG

Sequences:

>Translated_191_residues
MSAANATGEGQDAIAWNREMLPPGPEQDAVIKGITGEITEKGFVVASLDKLVNWGRTGSLWPMSFGLACCAVEMIHAYMP
RYDLDRMGIIPRGSPRQSDVMIVAGTLTNKMAPALRRVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDVY
VPGCPPTAEALIYGIFQLQKKIRRTGTILRG
>Mature_190_residues
SAANATGEGQDAIAWNREMLPPGPEQDAVIKGITGEITEKGFVVASLDKLVNWGRTGSLWPMSFGLACCAVEMIHAYMPR
YDLDRMGIIPRGSPRQSDVMIVAGTLTNKMAPALRRVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDVYV
PGCPPTAEALIYGIFQLQKKIRRTGTILRG

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=165, Percent_Identity=72.7272727272727, Blast_Score=256, Evalue=1e-68,
Organism=Escherichia coli, GI1788624, Length=161, Percent_Identity=49.6894409937888, Blast_Score=171, Evalue=2e-44,
Organism=Escherichia coli, GI1789074, Length=121, Percent_Identity=39.6694214876033, Blast_Score=97, Evalue=5e-22,
Organism=Escherichia coli, GI1788834, Length=122, Percent_Identity=39.344262295082, Blast_Score=94, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17509685, Length=146, Percent_Identity=68.4931506849315, Blast_Score=237, Evalue=3e-63,
Organism=Drosophila melanogaster, GI18859983, Length=154, Percent_Identity=73.3766233766234, Blast_Score=257, Evalue=4e-69,
Organism=Drosophila melanogaster, GI24642371, Length=154, Percent_Identity=73.3766233766234, Blast_Score=257, Evalue=4e-69,
Organism=Drosophila melanogaster, GI24651058, Length=146, Percent_Identity=77.3972602739726, Blast_Score=252, Evalue=1e-67,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOB2_GLUDA (A9HRU3)

Other databases:

- EMBL:   AM889285
- EMBL:   CP001189
- RefSeq:   YP_001603271.1
- RefSeq:   YP_002277671.1
- ProteinModelPortal:   A9HRU3
- SMR:   A9HRU3
- GeneID:   5791864
- GeneID:   6976772
- GenomeReviews:   AM889285_GR
- GenomeReviews:   CP001189_GR
- KEGG:   gdj:Gdia_3329
- HOGENOM:   HBG553221
- OMA:   GKPIGSN
- ProtClustDB:   PRK06411
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: =1.6.99.5

Molecular weight: Translated: 20905; Mature: 20773

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
7.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
7.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAANATGEGQDAIAWNREMLPPGPEQDAVIKGITGEITEKGFVVASLDKLVNWGRTGSL
CCCCCCCCCCCCCEEECCCCCCCCCCCCCEEECCCCCCCCCCEEEEEHHHHHCCCCCCCC
WPMSFGLACCAVEMIHAYMPRYDLDRMGIIPRGSPRQSDVMIVAGTLTNKMAPALRRVYD
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHH
QMAEPRWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALIYGIFQLQK
HHCCCEEEEEECCCCCCCCEEEEEHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHH
KIRRTGTILRG
HHHHHCCCCCC
>Mature Secondary Structure 
SAANATGEGQDAIAWNREMLPPGPEQDAVIKGITGEITEKGFVVASLDKLVNWGRTGSL
CCCCCCCCCCCCEEECCCCCCCCCCCCCEEECCCCCCCCCCEEEEEHHHHHCCCCCCCC
WPMSFGLACCAVEMIHAYMPRYDLDRMGIIPRGSPRQSDVMIVAGTLTNKMAPALRRVYD
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHH
QMAEPRWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALIYGIFQLQK
HHCCCEEEEEECCCCCCCCEEEEEHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHH
KIRRTGTILRG
HHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA