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The map label for this gene is proS [H]

Identifier: 162148795

GI number: 162148795

Start: 3113959

End: 3115389

Strand: Reverse

Name: proS [H]

Synonym: GDI_3024

Alternate gene names: 162148795

Gene position: 3115389-3113959 (Counterclockwise)

Preceding gene: 162148796

Following gene: 162148794

Centisome position: 78.99

GC content: 66.25

Gene sequence:

>1431_bases
GTGGACGGATACCTGCCGGTGGGGCGTGGCGGCGGCCCGGATTCTGGCGTATTCGTCTTCTCCTGTTTCACGATCCGCCC
GGCGTCCGCCGGTTTTTCCGAGGTTGATAACGGTATGCGTCTGTCCCGCGGCTTCCTGCCCACTCTCAAGGAGAATCCTG
CCGAGGCGCAGGTCATCTCGCACCGGCTCATGCTGCGGGCCGGTCTGATCCGCCAGACGGCGGCGGGGATTTATGCGTGG
CTGCCGGCCGGCTGGCGCGTGCTGCAGAACATCGCCCGGATCGTGCGCGAGGAACAGGACGCGATCGGCGCGCAGGAACT
GCTGATGCCCACCGTCCAGTCGGCGGAACTGTGGAAGCGGTCGGGCCGCTATGACGCCTACGGCCCCGAAATGCTGCGCA
TCCAGGACCGGCACGAGCGCGAACTGCTGTACGGCCCGACCAACGAGGAAATGATCACCGACCTGTTCGGCCAGGTGGTC
AAGTCGTACCGCGAACTGCCGCAGGTCCTGTACCATATCCAGTGGAAATTCCGTGACGAGGTCCGTCCGCGCTTCGGCGT
GATGCGCGGCCGCGAGTTCCTGATGAAGGACGCCTACAGCTTCGACCTCGATTACGCGTCGGCCGTCGATTCGTACCGGC
GGATGATGCTGGCCTACCTGCGCACCTTCCAGCGCCTGGGTGTCCGGGCCATCCCGATGGTGGCCGATACCGGCCCGATC
GGTGGCGAACTGAGCCACGAATTCCTGATCCTGGCGCCCACGGGCGAAAGCGGCGTGTTCTTCGACGGCGCGTTCGAGGA
ACAGGACTGGCTGTCCGACCCGGTGGATATCGACGACCGCGAATCGCTGGATGCGTTCTTCACCCGCATGACCTCGTTCT
ATGCCGCGACCGACGAAAAGCATGACGAGGCCGCCTGGGCCGACGTCCCCGCCGAGCGCCGGCGCGAGGGCAGGGGCATC
GAAGTCGGGCACATCTTCCATTTCGGCCGCAAATACACCGAATCGATGGATATCACCGTCAGCGGTCCGGACGGCGCGCC
GCTCTATCCGGAAATGGGCTCGTACGGCATCGGCGTGTCGCGCCTAGTCGCCGCGATCATCGAGGCCAGCCATGACGATA
ACGGCATCATCTGGCCGGACAGCGTGGCCCCCTTCCGCGCCGCGATCCTGAACCTGAAGACGGGGGATGCGGCCTGCGAC
GCGATCTGCGAACAGGTGTACGCCGCCGCCCCCGAATCCTTCCTGTATGACGACCGGGGCGAGCGCGCGGGCGTGAAGTT
CGCCGATGCCGACCTGATGGGCCATCCGTGGCAGGTCATCGTGGGCCCCAGGGGCGCCAAGGAGGGCAAGGTGGAACTGA
AGCGCCGCGCCGACGGCGCGCGGTTCGAACTGTCGGTGGATGACGCCCTGGCCCGAATCCTCGGGGGCTGA

Upstream 100 bases:

>100_bases
CGACCGGATCAGACGGGCGGGACTGTAGGAGGCTGCGGCGGGCCTGTCACCTGCCTCACGCAGTTGTTGAACGGGCCGGG
TTTCACGCCTGGGTGTAGGG

Downstream 100 bases:

>100_bases
TCGATGTTCGGTCCGTTCGAACGGGCGGTTGCCGGCCGGTACCTGCGTGCCCGCAAGGGTGAGCGGTTCGTGTCCGTCAT
CGCCATTTTCTCGCTGGTCG

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MDGYLPVGRGGGPDSGVFVFSCFTIRPASAGFSEVDNGMRLSRGFLPTLKENPAEAQVISHRLMLRAGLIRQTAAGIYAW
LPAGWRVLQNIARIVREEQDAIGAQELLMPTVQSAELWKRSGRYDAYGPEMLRIQDRHERELLYGPTNEEMITDLFGQVV
KSYRELPQVLYHIQWKFRDEVRPRFGVMRGREFLMKDAYSFDLDYASAVDSYRRMMLAYLRTFQRLGVRAIPMVADTGPI
GGELSHEFLILAPTGESGVFFDGAFEEQDWLSDPVDIDDRESLDAFFTRMTSFYAATDEKHDEAAWADVPAERRREGRGI
EVGHIFHFGRKYTESMDITVSGPDGAPLYPEMGSYGIGVSRLVAAIIEASHDDNGIIWPDSVAPFRAAILNLKTGDAACD
AICEQVYAAAPESFLYDDRGERAGVKFADADLMGHPWQVIVGPRGAKEGKVELKRRADGARFELSVDDALARILGG

Sequences:

>Translated_476_residues
MDGYLPVGRGGGPDSGVFVFSCFTIRPASAGFSEVDNGMRLSRGFLPTLKENPAEAQVISHRLMLRAGLIRQTAAGIYAW
LPAGWRVLQNIARIVREEQDAIGAQELLMPTVQSAELWKRSGRYDAYGPEMLRIQDRHERELLYGPTNEEMITDLFGQVV
KSYRELPQVLYHIQWKFRDEVRPRFGVMRGREFLMKDAYSFDLDYASAVDSYRRMMLAYLRTFQRLGVRAIPMVADTGPI
GGELSHEFLILAPTGESGVFFDGAFEEQDWLSDPVDIDDRESLDAFFTRMTSFYAATDEKHDEAAWADVPAERRREGRGI
EVGHIFHFGRKYTESMDITVSGPDGAPLYPEMGSYGIGVSRLVAAIIEASHDDNGIIWPDSVAPFRAAILNLKTGDAACD
AICEQVYAAAPESFLYDDRGERAGVKFADADLMGHPWQVIVGPRGAKEGKVELKRRADGARFELSVDDALARILGG
>Mature_476_residues
MDGYLPVGRGGGPDSGVFVFSCFTIRPASAGFSEVDNGMRLSRGFLPTLKENPAEAQVISHRLMLRAGLIRQTAAGIYAW
LPAGWRVLQNIARIVREEQDAIGAQELLMPTVQSAELWKRSGRYDAYGPEMLRIQDRHERELLYGPTNEEMITDLFGQVV
KSYRELPQVLYHIQWKFRDEVRPRFGVMRGREFLMKDAYSFDLDYASAVDSYRRMMLAYLRTFQRLGVRAIPMVADTGPI
GGELSHEFLILAPTGESGVFFDGAFEEQDWLSDPVDIDDRESLDAFFTRMTSFYAATDEKHDEAAWADVPAERRREGRGI
EVGHIFHFGRKYTESMDITVSGPDGAPLYPEMGSYGIGVSRLVAAIIEASHDDNGIIWPDSVAPFRAAILNLKTGDAACD
AICEQVYAAAPESFLYDDRGERAGVKFADADLMGHPWQVIVGPRGAKEGKVELKRRADGARFELSVDDALARILGG

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro) [H]

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=415, Percent_Identity=36.144578313253, Blast_Score=249, Evalue=4e-66,
Organism=Escherichia coli, GI1786392, Length=223, Percent_Identity=56.9506726457399, Blast_Score=258, Evalue=7e-70,
Organism=Caenorhabditis elegans, GI115532348, Length=398, Percent_Identity=32.6633165829146, Blast_Score=201, Evalue=6e-52,
Organism=Caenorhabditis elegans, GI193203271, Length=88, Percent_Identity=36.3636363636364, Blast_Score=78, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320931, Length=213, Percent_Identity=34.2723004694836, Blast_Score=147, Evalue=4e-36,
Organism=Drosophila melanogaster, GI24656200, Length=452, Percent_Identity=29.4247787610619, Blast_Score=213, Evalue=2e-55,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 53099; Mature: 53099

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGYLPVGRGGGPDSGVFVFSCFTIRPASAGFSEVDNGMRLSRGFLPTLKENPAEAQVIS
CCCCCCCCCCCCCCCCEEEEEEEEECCCCCCHHHHCCCCCHHCCCCCCCCCCCHHHHHHH
HRLMLRAGLIRQTAAGIYAWLPAGWRVLQNIARIVREEQDAIGAQELLMPTVQSAELWKR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
SGRYDAYGPEMLRIQDRHERELLYGPTNEEMITDLFGQVVKSYRELPQVLYHIQWKFRDE
CCCCCCCCCCEEEECCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VRPRFGVMRGREFLMKDAYSFDLDYASAVDSYRRMMLAYLRTFQRLGVRAIPMVADTGPI
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCC
GGELSHEFLILAPTGESGVFFDGAFEEQDWLSDPVDIDDRESLDAFFTRMTSFYAATDEK
CCCCCCCEEEEEECCCCCEEECCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
HDEAAWADVPAERRREGRGIEVGHIFHFGRKYTESMDITVSGPDGAPLYPEMGSYGIGVS
CCCCHHCCCCHHHHHCCCCCEEHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCHH
RLVAAIIEASHDDNGIIWPDSVAPFRAAILNLKTGDAACDAICEQVYAAAPESFLYDDRG
HHHHHHHHCCCCCCCEECCCCCCHHHHHHEECCCCHHHHHHHHHHHHHHCCHHHHCCCCC
ERAGVKFADADLMGHPWQVIVGPRGAKEGKVELKRRADGARFELSVDDALARILGG
CCCCCEEECCCCCCCCCEEEECCCCCCCCCHHHHHCCCCCEEEEEHHHHHHHHHCC
>Mature Secondary Structure
MDGYLPVGRGGGPDSGVFVFSCFTIRPASAGFSEVDNGMRLSRGFLPTLKENPAEAQVIS
CCCCCCCCCCCCCCCCEEEEEEEEECCCCCCHHHHCCCCCHHCCCCCCCCCCCHHHHHHH
HRLMLRAGLIRQTAAGIYAWLPAGWRVLQNIARIVREEQDAIGAQELLMPTVQSAELWKR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
SGRYDAYGPEMLRIQDRHERELLYGPTNEEMITDLFGQVVKSYRELPQVLYHIQWKFRDE
CCCCCCCCCCEEEECCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VRPRFGVMRGREFLMKDAYSFDLDYASAVDSYRRMMLAYLRTFQRLGVRAIPMVADTGPI
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCC
GGELSHEFLILAPTGESGVFFDGAFEEQDWLSDPVDIDDRESLDAFFTRMTSFYAATDEK
CCCCCCCEEEEEECCCCCEEECCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
HDEAAWADVPAERRREGRGIEVGHIFHFGRKYTESMDITVSGPDGAPLYPEMGSYGIGVS
CCCCHHCCCCHHHHHCCCCCEEHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCHH
RLVAAIIEASHDDNGIIWPDSVAPFRAAILNLKTGDAACDAICEQVYAAAPESFLYDDRG
HHHHHHHHCCCCCCCEECCCCCCHHHHHHEECCCCHHHHHHHHHHHHHHCCHHHHCCCCC
ERAGVKFADADLMGHPWQVIVGPRGAKEGKVELKRRADGARFELSVDDALARILGG
CCCCCEEECCCCCCCCCEEEECCCCCCCCCHHHHHCCCCCEEEEEHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA