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The map label for this gene is yjbJ [H]

Identifier: 162148568

GI number: 162148568

Start: 2879708

End: 2880379

Strand: Reverse

Name: yjbJ [H]

Synonym: GDI_2794

Alternate gene names: 162148568

Gene position: 2880379-2879708 (Counterclockwise)

Preceding gene: 162148569

Following gene: 162148563

Centisome position: 73.03

GC content: 61.46

Gene sequence:

>672_bases
ATGAGCCGCCGGTTTTTCATGTCCGTCGGCATGCTTGCCATGCTCGCGCTGCCGCTCTCGCCAGCCATCGCCCGCGCGCA
GGATTTCGACGATCTGGTGCGTCAAGCGGCGGAGCGGAACGCGATCTCGCCAGCATGGGTGAGGGCCGTCCTCCATGCCG
AAAGTGCTGGCGACCCGCACGCAGTTTCCGGCGCCGGTGCGATGGGGCTGATGCAGCTCATGCCCGGCACATGGAAGGAT
GTCCGCCGCACGCTCAATCTGGGTGCCGATCCCTTCGATCCGCACGACAATATCGCGGCTGGTGCAGCCTATCTGCGATG
GCTGCACGACCGCTATGGCGATGCGGGATTTCTTGCCGCCTACAATGCCGGACCTGGTCGCTATGACGATCATCTGGCGA
CTGGTCGGCCATTGCCTGCCGAGACGATTTCTTACGTGGAATTCGTCACACGGCTGATGAAAAACCGATCGTCTGACATT
CATCTGCACGTTTCCTTTTTGTCCTTTCCAGCTCGCTCCTGGGTGACGGCCGCGCTCTTCGTTTCAGGCCAGCCCGATGC
GTTTCGGGGCACCACGTTGCGTCATGTTTCTGCTCCCGAGAGCCTTTTCGCAGGGCATGCCGGAGGTAGGTCCGACATGG
TCCCGTTGAGGGGATGGGATCAGATCCAGTGA

Upstream 100 bases:

>100_bases
TCATGCAACCGATCCGCGCCACGCTGTTCCAGGCCGGGCGCGGCGGGCGTGAGTGGCAACTGGTCTGGAACCGGCCTGCA
AAACGTCCGGGAGGTCGGGA

Downstream 100 bases:

>100_bases
GGCGTGAAAACCCCGGAAAGCCAACAGAAAAGGGAGTGCCAGCCGTAATCGGGCCTGAGCGGTCGTCGTCGATTGGCGGT
CTCCCGGGCCGGAAAGAAGG

Product: lytic transglycosylase, catalytic

Products: N-Acetylmuramic Acid Residues; N-Acetylglucosamine Residues [C]

Alternate protein names: NA

Number of amino acids: Translated: 223; Mature: 222

Protein sequence:

>223_residues
MSRRFFMSVGMLAMLALPLSPAIARAQDFDDLVRQAAERNAISPAWVRAVLHAESAGDPHAVSGAGAMGLMQLMPGTWKD
VRRTLNLGADPFDPHDNIAAGAAYLRWLHDRYGDAGFLAAYNAGPGRYDDHLATGRPLPAETISYVEFVTRLMKNRSSDI
HLHVSFLSFPARSWVTAALFVSGQPDAFRGTTLRHVSAPESLFAGHAGGRSDMVPLRGWDQIQ

Sequences:

>Translated_223_residues
MSRRFFMSVGMLAMLALPLSPAIARAQDFDDLVRQAAERNAISPAWVRAVLHAESAGDPHAVSGAGAMGLMQLMPGTWKD
VRRTLNLGADPFDPHDNIAAGAAYLRWLHDRYGDAGFLAAYNAGPGRYDDHLATGRPLPAETISYVEFVTRLMKNRSSDI
HLHVSFLSFPARSWVTAALFVSGQPDAFRGTTLRHVSAPESLFAGHAGGRSDMVPLRGWDQIQ
>Mature_222_residues
SRRFFMSVGMLAMLALPLSPAIARAQDFDDLVRQAAERNAISPAWVRAVLHAESAGDPHAVSGAGAMGLMQLMPGTWKDV
RRTLNLGADPFDPHDNIAAGAAYLRWLHDRYGDAGFLAAYNAGPGRYDDHLATGRPLPAETISYVEFVTRLMKNRSSDIH
LHVSFLSFPARSWVTAALFVSGQPDAFRGTTLRHVSAPESLFAGHAGGRSDMVPLRGWDQIQ

Specific function: Murein-Degrading Enzyme. May Play A Role In Recycling Of Muropeptides During Cell Elongation And/Or Cell Division (By Similarity). [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Attached To The Membrane By A Lipid Anchor [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=109, Percent_Identity=34.8623853211009, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 24298; Mature: 24167

Theoretical pI: Translated: 8.03; Mature: 8.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRFFMSVGMLAMLALPLSPAIARAQDFDDLVRQAAERNAISPAWVRAVLHAESAGDPH
CCCHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCC
AVSGAGAMGLMQLMPGTWKDVRRTLNLGADPFDPHDNIAAGAAYLRWLHDRYGDAGFLAA
CCCCCCHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEE
YNAGPGRYDDHLATGRPLPAETISYVEFVTRLMKNRSSDIHLHVSFLSFPARSWVTAALF
ECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEHHCCHHHHHEEHEE
VSGQPDAFRGTTLRHVSAPESLFAGHAGGRSDMVPLRGWDQIQ
ECCCCCCCCCCEEEECCCCHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SRRFFMSVGMLAMLALPLSPAIARAQDFDDLVRQAAERNAISPAWVRAVLHAESAGDPH
CCHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCC
AVSGAGAMGLMQLMPGTWKDVRRTLNLGADPFDPHDNIAAGAAYLRWLHDRYGDAGFLAA
CCCCCCHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEE
YNAGPGRYDDHLATGRPLPAETISYVEFVTRLMKNRSSDIHLHVSFLSFPARSWVTAALF
ECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEHHCCHHHHHEEHEE
VSGQPDAFRGTTLRHVSAPESLFAGHAGGRSDMVPLRGWDQIQ
ECCCCCCCCCCEEEECCCCHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]