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The map label for this gene is dht [C]

Identifier: 162148496

GI number: 162148496

Start: 2813653

End: 2815047

Strand: Direct

Name: dht [C]

Synonym: GDI_2717

Alternate gene names: 162148496

Gene position: 2813653-2815047 (Clockwise)

Preceding gene: 162148495

Following gene: 162148500

Centisome position: 71.34

GC content: 62.8

Gene sequence:

>1395_bases
ATGACCTTCGACATCGTCATCCGCAACGGCACCATCGTGACCGCCAGCGACGTGTTTACCGCCGATATCGGCATTACCGG
CGAAACGATCGACACGATCGGGCACGGTCTGACCGGCCACCATGTCGTGGACGCCACCGGGCTGCTGGTCATGCCGGGCG
GCATCGACGGTCATTGCCATATCGAGCAGGAAGAGGCCGACGGCACGGTGCATGAGGACGATTTCGCTTCGGCAAGCGCG
TCCGCACTGCTGGGCGGCACGACGACGGTGGTGTGTTTCGCGTCCCATGTGCCCGGACGCAACATCGTCACACAGTTCGA
GGATTATCTCCGGCGGGGGCAGAATTCGCGCATCGATTTCGCCGTCCACCAGATCGTCACGCGTACCGACGCGGATACGA
TCGAAAAGGGTATTCCCGAAATCGCCCGCCGGGGCGTGGCGGGGCTGAAGGTCTTCATGACCTACGACGATTTCCATCTG
ACGGACGGACAGTTCCTACGCGTTCTCGACGCGGCGAAGGCCAGCGGCCTCGTGGTTTCGGTCCATTGCGAAAATTACGA
CGCGATCCGCCACATGATCGAAGCCCATCTCGCCGCCGGACGAACCGACGCCCACGCCCACGCCACCTCCCGCCCCCCCT
GCCTGGAACGCGAGGCCACCTATCGCGCCATGACCCTGGCCGAACTCGTCGACCACGACATCCAGATCTTCCATGTTTCA
TGCCCGGAAGTCGCCGAGGAAATCGCGCGCGCCCGTCACCGCGGCGTCAAGGTCTCCGCGGAAACCTGCCCGCATTATCT
TTCCCTCACGGAAGCCGATCTGAAGCGGAACGGATTCGAGGGCGCAAAATATATCTGCAGCCCCCCTCTGCGCACCCAGG
CCGACCAGGACGGCATGTGGTCGTATCTGCGCGATGGAGTGATCGGCATCGTATCGTCCGATCATTGCGGCTACAGCTTG
GAAGGAACGTCCGGCAAAGCACGCCATGGCCGCGACGCGGATTTTTCACTGATCCCCAACGGGATGCCCGGGCTGGGAAC
CAGAATGGCCGTCCTGTTCGATGCCGGTGTAAACAGCCAGAAGATCGGCCTGACGGATTTCGTCCGCCTGACGGCCACCG
CACCGGCCCAGCGATATGGCCTTTATCCCCGCAAGGGCACGATCGCCCCGGGCAGCGACGCGGACCTGGTCCTCTGGGAC
CGGACACAGCAGGTCAGGATCACCAATGACCTGTTCCAAAGCAGGATCGACTACACACCATTCGAGGGACGCCTCGTCAC
CGGTTGGCCCGCCATGGTGCTTGCACGCGGCAGGATAGCCGTCGAGAACGGCCAGGTACTCCGCAAACCCACGATGGGCC
GTTTCCTTAAAGCCCGTTCGGGACCGCAGGATTGA

Upstream 100 bases:

>100_bases
CCATGCTGACCCACCTGCGCAACGTTGCGACAGACTGCATGGACATCCTTCGCGGCGGCTAGCGCTCTGCCCCGACCTTT
CCCACGCCAGGAATCTTCCA

Downstream 100 bases:

>100_bases
ACATGCCTGCCGCCCCTGCCCCAATGTGCCCTGGGTTCCGAATTAAGGTTACGGCTTGCTCCCGAAGGTCGAACCGCCGA
ATGGTAGAGTTTCCGACCTG

Product: dihydropyrimidinase

Products: 3-ureidopropanoate

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MTFDIVIRNGTIVTASDVFTADIGITGETIDTIGHGLTGHHVVDATGLLVMPGGIDGHCHIEQEEADGTVHEDDFASASA
SALLGGTTTVVCFASHVPGRNIVTQFEDYLRRGQNSRIDFAVHQIVTRTDADTIEKGIPEIARRGVAGLKVFMTYDDFHL
TDGQFLRVLDAAKASGLVVSVHCENYDAIRHMIEAHLAAGRTDAHAHATSRPPCLEREATYRAMTLAELVDHDIQIFHVS
CPEVAEEIARARHRGVKVSAETCPHYLSLTEADLKRNGFEGAKYICSPPLRTQADQDGMWSYLRDGVIGIVSSDHCGYSL
EGTSGKARHGRDADFSLIPNGMPGLGTRMAVLFDAGVNSQKIGLTDFVRLTATAPAQRYGLYPRKGTIAPGSDADLVLWD
RTQQVRITNDLFQSRIDYTPFEGRLVTGWPAMVLARGRIAVENGQVLRKPTMGRFLKARSGPQD

Sequences:

>Translated_464_residues
MTFDIVIRNGTIVTASDVFTADIGITGETIDTIGHGLTGHHVVDATGLLVMPGGIDGHCHIEQEEADGTVHEDDFASASA
SALLGGTTTVVCFASHVPGRNIVTQFEDYLRRGQNSRIDFAVHQIVTRTDADTIEKGIPEIARRGVAGLKVFMTYDDFHL
TDGQFLRVLDAAKASGLVVSVHCENYDAIRHMIEAHLAAGRTDAHAHATSRPPCLEREATYRAMTLAELVDHDIQIFHVS
CPEVAEEIARARHRGVKVSAETCPHYLSLTEADLKRNGFEGAKYICSPPLRTQADQDGMWSYLRDGVIGIVSSDHCGYSL
EGTSGKARHGRDADFSLIPNGMPGLGTRMAVLFDAGVNSQKIGLTDFVRLTATAPAQRYGLYPRKGTIAPGSDADLVLWD
RTQQVRITNDLFQSRIDYTPFEGRLVTGWPAMVLARGRIAVENGQVLRKPTMGRFLKARSGPQD
>Mature_463_residues
TFDIVIRNGTIVTASDVFTADIGITGETIDTIGHGLTGHHVVDATGLLVMPGGIDGHCHIEQEEADGTVHEDDFASASAS
ALLGGTTTVVCFASHVPGRNIVTQFEDYLRRGQNSRIDFAVHQIVTRTDADTIEKGIPEIARRGVAGLKVFMTYDDFHLT
DGQFLRVLDAAKASGLVVSVHCENYDAIRHMIEAHLAAGRTDAHAHATSRPPCLEREATYRAMTLAELVDHDIQIFHVSC
PEVAEEIARARHRGVKVSAETCPHYLSLTEADLKRNGFEGAKYICSPPLRTQADQDGMWSYLRDGVIGIVSSDHCGYSLE
GTSGKARHGRDADFSLIPNGMPGLGTRMAVLFDAGVNSQKIGLTDFVRLTATAPAQRYGLYPRKGTIAPGSDADLVLWDR
TQQVRITNDLFQSRIDYTPFEGRLVTGWPAMVLARGRIAVENGQVLRKPTMGRFLKARSGPQD

Specific function: Catalyzes the stereospecific hydrolysis of the cyclic amide bond of D-hydantoin. Has no activity on dihydropyrimidines [H]

COG id: COG0044

COG function: function code F; Dihydroorotase and related cyclic amidohydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4503051, Length=473, Percent_Identity=33.1923890063425, Blast_Score=230, Evalue=2e-60,
Organism=Homo sapiens, GI62422571, Length=473, Percent_Identity=33.1923890063425, Blast_Score=229, Evalue=6e-60,
Organism=Homo sapiens, GI190194363, Length=472, Percent_Identity=31.1440677966102, Blast_Score=223, Evalue=4e-58,
Organism=Homo sapiens, GI4503377, Length=478, Percent_Identity=33.0543933054393, Blast_Score=215, Evalue=9e-56,
Organism=Homo sapiens, GI4503375, Length=464, Percent_Identity=32.5431034482759, Blast_Score=214, Evalue=1e-55,
Organism=Homo sapiens, GI4503379, Length=460, Percent_Identity=31.5217391304348, Blast_Score=207, Evalue=2e-53,
Organism=Homo sapiens, GI19923821, Length=467, Percent_Identity=32.3340471092077, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI18105007, Length=422, Percent_Identity=27.0142180094787, Blast_Score=108, Evalue=9e-24,
Organism=Escherichia coli, GI87082175, Length=465, Percent_Identity=34.4086021505376, Blast_Score=241, Evalue=9e-65,
Organism=Escherichia coli, GI1786722, Length=470, Percent_Identity=31.2765957446809, Blast_Score=198, Evalue=7e-52,
Organism=Caenorhabditis elegans, GI17539558, Length=464, Percent_Identity=32.3275862068966, Blast_Score=209, Evalue=3e-54,
Organism=Caenorhabditis elegans, GI71989490, Length=468, Percent_Identity=30.7692307692308, Blast_Score=202, Evalue=4e-52,
Organism=Caenorhabditis elegans, GI86575075, Length=461, Percent_Identity=27.765726681128, Blast_Score=145, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI193204318, Length=462, Percent_Identity=24.2424242424242, Blast_Score=106, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6322218, Length=476, Percent_Identity=24.7899159663866, Blast_Score=124, Evalue=4e-29,
Organism=Drosophila melanogaster, GI221377917, Length=465, Percent_Identity=32.0430107526882, Blast_Score=209, Evalue=4e-54,
Organism=Drosophila melanogaster, GI17137462, Length=463, Percent_Identity=31.317494600432, Blast_Score=195, Evalue=6e-50,
Organism=Drosophila melanogaster, GI24642586, Length=426, Percent_Identity=27.6995305164319, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24644287, Length=298, Percent_Identity=31.2080536912752, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24644289, Length=234, Percent_Identity=30.7692307692308, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI18859883, Length=256, Percent_Identity=28.515625, Blast_Score=96, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR011778
- InterPro:   IPR011059 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: 3.5.2.2

Molecular weight: Translated: 50491; Mature: 50359

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFDIVIRNGTIVTASDVFTADIGITGETIDTIGHGLTGHHVVDATGLLVMPGGIDGHCH
CEEEEEEECCEEEEECCEEEEECCCCCCHHHHHCCCCCCCEEEECCCEEEECCCCCCEEE
IEQEEADGTVHEDDFASASASALLGGTTTVVCFASHVPGRNIVTQFEDYLRRGQNSRIDF
EEHHCCCCCCCCCHHCCCCCHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCHH
AVHQIVTRTDADTIEKGIPEIARRGVAGLKVFMTYDDFHLTDGQFLRVLDAAKASGLVVS
HHHHHHHHCCHHHHHHCCHHHHHCCCCEEEEEEEECCEECCCCHHHHHHHHHCCCCEEEE
VHCENYDAIRHMIEAHLAAGRTDAHAHATSRPPCLEREATYRAMTLAELVDHDIQIFHVS
EEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEC
CPEVAEEIARARHRGVKVSAETCPHYLSLTEADLKRNGFEGAKYICSPPLRTQADQDGMW
CHHHHHHHHHHHHCCCEEEHHHCCCHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCHHH
SYLRDGVIGIVSSDHCGYSLEGTSGKARHGRDADFSLIPNGMPGLGTRMAVLFDAGVNSQ
HHHHCCEEEEEECCCCCEEEECCCCCCCCCCCCCCEECCCCCCCCCCEEEEEEECCCCCC
KIGLTDFVRLTATAPAQRYGLYPRKGTIAPGSDADLVLWDRTQQVRITNDLFQSRIDYTP
CCCHHHHEEEEECCCHHHHCCCCCCCCCCCCCCCCEEEECCCCEEEEHHHHHHHHCCCCC
FEGRLVTGWPAMVLARGRIAVENGQVLRKPTMGRFLKARSGPQD
CCCEEEECCHHHHHCCCEEEEECCCEEECCCHHHHHHCCCCCCC
>Mature Secondary Structure 
TFDIVIRNGTIVTASDVFTADIGITGETIDTIGHGLTGHHVVDATGLLVMPGGIDGHCH
EEEEEEECCEEEEECCEEEEECCCCCCHHHHHCCCCCCCEEEECCCEEEECCCCCCEEE
IEQEEADGTVHEDDFASASASALLGGTTTVVCFASHVPGRNIVTQFEDYLRRGQNSRIDF
EEHHCCCCCCCCCHHCCCCCHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCHH
AVHQIVTRTDADTIEKGIPEIARRGVAGLKVFMTYDDFHLTDGQFLRVLDAAKASGLVVS
HHHHHHHHCCHHHHHHCCHHHHHCCCCEEEEEEEECCEECCCCHHHHHHHHHCCCCEEEE
VHCENYDAIRHMIEAHLAAGRTDAHAHATSRPPCLEREATYRAMTLAELVDHDIQIFHVS
EEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEC
CPEVAEEIARARHRGVKVSAETCPHYLSLTEADLKRNGFEGAKYICSPPLRTQADQDGMW
CHHHHHHHHHHHHCCCEEEHHHCCCHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCHHH
SYLRDGVIGIVSSDHCGYSLEGTSGKARHGRDADFSLIPNGMPGLGTRMAVLFDAGVNSQ
HHHHCCEEEEEECCCCCEEEECCCCCCCCCCCCCCEECCCCCCCCCCEEEEEEECCCCCC
KIGLTDFVRLTATAPAQRYGLYPRKGTIAPGSDADLVLWDRTQQVRITNDLFQSRIDYTP
CCCHHHHEEEEECCCHHHHCCCCCCCCCCCCCCCCEEEECCCCEEEEHHHHHHHHCCCCC
FEGRLVTGWPAMVLARGRIAVENGQVLRKPTMGRFLKARSGPQD
CCCEEEECCHHHHHCCCEEEEECCCEEECCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 5,6-dihydrouracil; H2O

Specific reaction: 5,6-dihydrouracil + H2O = 3-ureidopropanoate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7765480 [H]