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The map label for this gene is metF [H]

Identifier: 162148358

GI number: 162148358

Start: 2669715

End: 2670467

Strand: Direct

Name: metF [H]

Synonym: GDI_2575

Alternate gene names: 162148358

Gene position: 2669715-2670467 (Clockwise)

Preceding gene: 162148357

Following gene: 162148359

Centisome position: 67.69

GC content: 69.06

Gene sequence:

>753_bases
GTGCCCCTTGCACCGCGTTTCATGTCGGTCACCTATGGCGCCGGGGGATCGACGCGCGACCGCACGCTGGACATCGTCGC
GTCGCTGGTTCGCGAAACCACCGTGCCGGTGGCTGGACACCTGACCTGCGTCGACGCCTCGCGCGATCAGGTCGATGCGG
TGGCGCGCGCTTATTGGGACGCGGGCGTGCGGCATATCGTGGCCCTGCGGGGCGACCCGGCCGAGGCGGGTGCCGCGTAC
GTACCGCATCCCGACGGATATGCGAACGCGGCGGCGCTGGTCGCCGGCCTGCACCGCATCGCGCCGTTCGAGATTTCGGT
CGCCGCCTATCCCGAAACCCATCCCGCCGCCGTCAGCGCCGCCGCCGACCTGGACAATCTGAAGGCCAAGATCGATGCCG
GCGCCACGCGGGCGATCACGCAATTCTTCTTCGAGTCCGAAACGTTCCTGCGCTTCCGCGACCATGCCGCTTCCGCCGGG
ATCACGGCCGAAATCGTGCCCGGCATCCTGCCCGTGGGAAATATCGCCCAGGCCTATCGCTTTGCCGACATGTGCGGCGC
ACGCGTTCCGGACTGGATGCGGCGCCTGTTCGACGGGCTGGACGACCAGCCCGGCGCCCGTGCCATCGTGACGACGACGG
TCGCGACCGAACTGTGCCGGCGCCTGCGCGAGGAGGGGGTCGATCATTTCCATATCTACACCCTCAACCGGGCCGACGCC
TCGCTGGCGCTGTGCCGGCTGCTGGGCCGGTAA

Upstream 100 bases:

>100_bases
GTCCGCCGACACACCGTCCCCCTTGGGAATGTCGTTCGAATTCTTCCCGCCCAGGACTGACAGGATGGCGGAGACGCTGC
GCCAGACCGTGGCCAGCCTG

Downstream 100 bases:

>100_bases
CGGAAAAAAACGCGGCGGGCCTGCAACTTGGGGTGGGTGTCGGGCGCTTGATCATGCGATGGCCTCCCCGATCCGCCCTG
CCATGAAAGTCCCCGACATG

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MPLAPRFMSVTYGAGGSTRDRTLDIVASLVRETTVPVAGHLTCVDASRDQVDAVARAYWDAGVRHIVALRGDPAEAGAAY
VPHPDGYANAAALVAGLHRIAPFEISVAAYPETHPAAVSAAADLDNLKAKIDAGATRAITQFFFESETFLRFRDHAASAG
ITAEIVPGILPVGNIAQAYRFADMCGARVPDWMRRLFDGLDDQPGARAIVTTTVATELCRRLREEGVDHFHIYTLNRADA
SLALCRLLGR

Sequences:

>Translated_250_residues
MPLAPRFMSVTYGAGGSTRDRTLDIVASLVRETTVPVAGHLTCVDASRDQVDAVARAYWDAGVRHIVALRGDPAEAGAAY
VPHPDGYANAAALVAGLHRIAPFEISVAAYPETHPAAVSAAADLDNLKAKIDAGATRAITQFFFESETFLRFRDHAASAG
ITAEIVPGILPVGNIAQAYRFADMCGARVPDWMRRLFDGLDDQPGARAIVTTTVATELCRRLREEGVDHFHIYTLNRADA
SLALCRLLGR
>Mature_249_residues
PLAPRFMSVTYGAGGSTRDRTLDIVASLVRETTVPVAGHLTCVDASRDQVDAVARAYWDAGVRHIVALRGDPAEAGAAYV
PHPDGYANAAALVAGLHRIAPFEISVAAYPETHPAAVSAAADLDNLKAKIDAGATRAITQFFFESETFLRFRDHAASAGI
TAEIVPGILPVGNIAQAYRFADMCGARVPDWMRRLFDGLDDQPGARAIVTTTVATELCRRLREEGVDHFHIYTLNRADAS
LALCRLLGR

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=253, Percent_Identity=32.4110671936759, Blast_Score=122, Evalue=4e-28,
Organism=Escherichia coli, GI1790377, Length=248, Percent_Identity=54.0322580645161, Blast_Score=268, Evalue=3e-73,
Organism=Caenorhabditis elegans, GI25147436, Length=229, Percent_Identity=33.1877729257642, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI25147441, Length=229, Percent_Identity=33.1877729257642, Blast_Score=105, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6321313, Length=250, Percent_Identity=28, Blast_Score=112, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6325234, Length=256, Percent_Identity=33.203125, Blast_Score=106, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 26828; Mature: 26696

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLAPRFMSVTYGAGGSTRDRTLDIVASLVRETTVPVAGHLTCVDASRDQVDAVARAYWD
CCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHH
AGVRHIVALRGDPAEAGAAYVPHPDGYANAAALVAGLHRIAPFEISVAAYPETHPAAVSA
HHHHEEEEECCCCHHCCCEECCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHH
AADLDNLKAKIDAGATRAITQFFFESETFLRFRDHAASAGITAEIVPGILPVGNIAQAYR
HHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHCCCCCCCHHHHHHH
FADMCGARVPDWMRRLFDGLDDQPGARAIVTTTVATELCRRLREEGVDHFHIYTLNRADA
HHHHHCCCCHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCEEEEEEECCCHH
SLALCRLLGR
HHHHHHHHCC
>Mature Secondary Structure 
PLAPRFMSVTYGAGGSTRDRTLDIVASLVRETTVPVAGHLTCVDASRDQVDAVARAYWD
CCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHH
AGVRHIVALRGDPAEAGAAYVPHPDGYANAAALVAGLHRIAPFEISVAAYPETHPAAVSA
HHHHEEEEECCCCHHCCCEECCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHH
AADLDNLKAKIDAGATRAITQFFFESETFLRFRDHAASAGITAEIVPGILPVGNIAQAYR
HHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHCCCCCCCHHHHHHH
FADMCGARVPDWMRRLFDGLDDQPGARAIVTTTVATELCRRLREEGVDHFHIYTLNRADA
HHHHHCCCCHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCEEEEEEECCCHH
SLALCRLLGR
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA