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The map label for this gene is nuoF [H]

Identifier: 162148250

GI number: 162148250

Start: 2557945

End: 2559231

Strand: Reverse

Name: nuoF [H]

Synonym: GDI_2467

Alternate gene names: 162148250

Gene position: 2559231-2557945 (Counterclockwise)

Preceding gene: 162148251

Following gene: 162148249

Centisome position: 64.89

GC content: 71.17

Gene sequence:

>1287_bases
ATGAGCGAACGCCCCCTGACCGCCATGATCGACGCCGCCGGCCCGCCGGACTGCGCCGCCTATGGCCGCGCCGGCGGCTG
GCAGGGGCTGCGGCGCGCGCTGCGCGACCTGACGCCGGAGGCCGTCATTGCCGAGGTCACGGATTCACGCCTGCGCGGGC
GCGGCGGAGCCGGGTTCGGCACCGGGCAGAAATGGAGCTTCGTACCCAGGACCCCGGCCGGGGACCAGCGCAAATACCTG
GTCGCCAATGCCGACGAGATGGAGCCCGGCACCTTCAAGGACCGCATCCTGCTGGAGGGCAATCCCCTGCAACTGATCGA
GGGGATGATCGTGGCGGGATATGCCATCCAGGCCGGCGAGGGCGTGATCTTCCTGCGCGGGGAATATCACCTGGCGCTGG
ACCGCCTGACCCGCGCGATCGACGAGGCCCGGGCCGTCGGCTGGCTGGGGCGGGACATCGCGGGCTCGGGCTTCGATTTC
GACATCCATGTCCATGCCAGCGGCGGCCGCTATATCTGCGGCGAGGAAACGGCGCTGCTGACGGCGCTGGAGGGCAGCCG
CGCGGTGCCGCGCAGCAAGCCGCCCTTTCCGCAGGTCGGCGGCCTGTGGGGCGCGCCCTCGGTCGTCAACAATGTCGAAA
CCCTGTGCAACGTGCCACACATCCTGGCCAATGGCGCCGCGTGGTTCCGCGCGCTGGCCATCGGGCCGGATGGCGGGACC
AAGCTGTTCGGCGTCAGCGGCCGGGTGAAGCGGCCGGGCGCGTGGGAACTGCCGATGGGCACCCCGCTGCGTGAGATCAT
CGAGGAGCATGCCGGCGGCATGCGCGACGGCTACGGCCTGCGCGCGCTGCTGCCGGGCGGGGCCTCCACCGCCTTTCTCG
ACGGCGGGGCGATCGACATCGCCATGGACTACGCCACCGTCGAGAAGGCCGGCAGCCGGCTGGGCACGGGGCTTGCGATC
CTGCTGGACGACCGGACCTGTCCGGTCGGGATGCTGCGCAACCTGGAACATTTCTTCGCCCAGGAATCCTGCGGCTGGTG
CACGCCCTGCCGCGACGGGCTGCCCTGGGTGGAACGGCTGCTGGACGCGATCGAGGAGGGCAGGGGCGAGGATGGCGACC
TGGACCTGCTGGCCCATCATGCCCGCATGATCGGCCCGATGGGCCGCACCTTCTGCGCGCATGCCCCCGGCGCCATCGCG
CCGCTGTCCAGTGGACTGAACCTGTTCCGGGCGGAGTTCGAGGCGCATCTGCGCCTCGGCCGCTGCCCGCATCGCCACGC
GGCATAA

Upstream 100 bases:

>100_bases
GCGACCACGCGCCGGCCCTGCTGGTGGACCGGACGCTGCATGGCGATGTGGACGAGGCCGGGATCGACCGCATCGCCGAC
AACGTGCGGGGGCACCCGGC

Downstream 100 bases:

>100_bases
GGGGACGCCATGGCCACGATCCGGATCGACGGACGAGACTGCCCCGCCCGGCTGGATGAAAACCTGCTGCAGGCGTGCCT
GGAAAGCGGCGCGGACCTGC

Product: NADH-quinone oxidoreductase subunit F

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F; NDH-1 subunit F; NUO6 [H]

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MSERPLTAMIDAAGPPDCAAYGRAGGWQGLRRALRDLTPEAVIAEVTDSRLRGRGGAGFGTGQKWSFVPRTPAGDQRKYL
VANADEMEPGTFKDRILLEGNPLQLIEGMIVAGYAIQAGEGVIFLRGEYHLALDRLTRAIDEARAVGWLGRDIAGSGFDF
DIHVHASGGRYICGEETALLTALEGSRAVPRSKPPFPQVGGLWGAPSVVNNVETLCNVPHILANGAAWFRALAIGPDGGT
KLFGVSGRVKRPGAWELPMGTPLREIIEEHAGGMRDGYGLRALLPGGASTAFLDGGAIDIAMDYATVEKAGSRLGTGLAI
LLDDRTCPVGMLRNLEHFFAQESCGWCTPCRDGLPWVERLLDAIEEGRGEDGDLDLLAHHARMIGPMGRTFCAHAPGAIA
PLSSGLNLFRAEFEAHLRLGRCPHRHAA

Sequences:

>Translated_428_residues
MSERPLTAMIDAAGPPDCAAYGRAGGWQGLRRALRDLTPEAVIAEVTDSRLRGRGGAGFGTGQKWSFVPRTPAGDQRKYL
VANADEMEPGTFKDRILLEGNPLQLIEGMIVAGYAIQAGEGVIFLRGEYHLALDRLTRAIDEARAVGWLGRDIAGSGFDF
DIHVHASGGRYICGEETALLTALEGSRAVPRSKPPFPQVGGLWGAPSVVNNVETLCNVPHILANGAAWFRALAIGPDGGT
KLFGVSGRVKRPGAWELPMGTPLREIIEEHAGGMRDGYGLRALLPGGASTAFLDGGAIDIAMDYATVEKAGSRLGTGLAI
LLDDRTCPVGMLRNLEHFFAQESCGWCTPCRDGLPWVERLLDAIEEGRGEDGDLDLLAHHARMIGPMGRTFCAHAPGAIA
PLSSGLNLFRAEFEAHLRLGRCPHRHAA
>Mature_427_residues
SERPLTAMIDAAGPPDCAAYGRAGGWQGLRRALRDLTPEAVIAEVTDSRLRGRGGAGFGTGQKWSFVPRTPAGDQRKYLV
ANADEMEPGTFKDRILLEGNPLQLIEGMIVAGYAIQAGEGVIFLRGEYHLALDRLTRAIDEARAVGWLGRDIAGSGFDFD
IHVHASGGRYICGEETALLTALEGSRAVPRSKPPFPQVGGLWGAPSVVNNVETLCNVPHILANGAAWFRALAIGPDGGTK
LFGVSGRVKRPGAWELPMGTPLREIIEEHAGGMRDGYGLRALLPGGASTAFLDGGAIDIAMDYATVEKAGSRLGTGLAIL
LDDRTCPVGMLRNLEHFFAQESCGWCTPCRDGLPWVERLLDAIEEGRGEDGDLDLLAHHARMIGPMGRTFCAHAPGAIAP
LSSGLNLFRAEFEAHLRLGRCPHRHAA

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI20149568, Length=382, Percent_Identity=41.6230366492147, Blast_Score=291, Evalue=1e-78,
Organism=Homo sapiens, GI260656005, Length=382, Percent_Identity=41.6230366492147, Blast_Score=290, Evalue=1e-78,
Organism=Escherichia coli, GI1788620, Length=415, Percent_Identity=54.9397590361446, Blast_Score=466, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI17531727, Length=378, Percent_Identity=40.7407407407407, Blast_Score=289, Evalue=2e-78,
Organism=Drosophila melanogaster, GI19920794, Length=380, Percent_Identity=40.7894736842105, Blast_Score=298, Evalue=7e-81,
Organism=Drosophila melanogaster, GI45550452, Length=379, Percent_Identity=42.4802110817942, Blast_Score=294, Evalue=7e-80,
Organism=Drosophila melanogaster, GI24653819, Length=384, Percent_Identity=35.6770833333333, Blast_Score=241, Evalue=5e-64,
Organism=Drosophila melanogaster, GI24653817, Length=360, Percent_Identity=33.8888888888889, Blast_Score=215, Evalue=6e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 45698; Mature: 45566

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSERPLTAMIDAAGPPDCAAYGRAGGWQGLRRALRDLTPEAVIAEVTDSRLRGRGGAGFG
CCCCCCEEEEECCCCCCHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCC
TGQKWSFVPRTPAGDQRKYLVANADEMEPGTFKDRILLEGNPLQLIEGMIVAGYAIQAGE
CCCCCEECCCCCCCCCCEEEEECCCCCCCCCCCCEEEECCCHHHHHHHHHHCCEEEECCC
GVIFLRGEYHLALDRLTRAIDEARAVGWLGRDIAGSGFDFDIHVHASGGRYICGEETALL
CEEEEECCHHHHHHHHHHHHHHHHHHHHHCHHCCCCCCEEEEEEECCCCEEEECCCCEEE
TALEGSRAVPRSKPPFPQVGGLWGAPSVVNNVETLCNVPHILANGAAWFRALAIGPDGGT
EEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHCCHHHHEEEEECCCCCC
KLFGVSGRVKRPGAWELPMGTPLREIIEEHAGGMRDGYGLRALLPGGASTAFLDGGAIDI
EEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCEEEECCCEEEE
AMDYATVEKAGSRLGTGLAILLDDRTCPVGMLRNLEHFFAQESCGWCTPCRDGLPWVERL
EEHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHH
LDAIEEGRGEDGDLDLLAHHARMIGPMGRTFCAHAPGAIAPLSSGLNLFRAEFEAHLRLG
HHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHCHHHHHHHHHHHHHHC
RCPHRHAA
CCCCCCCC
>Mature Secondary Structure 
SERPLTAMIDAAGPPDCAAYGRAGGWQGLRRALRDLTPEAVIAEVTDSRLRGRGGAGFG
CCCCCEEEEECCCCCCHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCC
TGQKWSFVPRTPAGDQRKYLVANADEMEPGTFKDRILLEGNPLQLIEGMIVAGYAIQAGE
CCCCCEECCCCCCCCCCEEEEECCCCCCCCCCCCEEEECCCHHHHHHHHHHCCEEEECCC
GVIFLRGEYHLALDRLTRAIDEARAVGWLGRDIAGSGFDFDIHVHASGGRYICGEETALL
CEEEEECCHHHHHHHHHHHHHHHHHHHHHCHHCCCCCCEEEEEEECCCCEEEECCCCEEE
TALEGSRAVPRSKPPFPQVGGLWGAPSVVNNVETLCNVPHILANGAAWFRALAIGPDGGT
EEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHCCHHHHEEEEECCCCCC
KLFGVSGRVKRPGAWELPMGTPLREIIEEHAGGMRDGYGLRALLPGGASTAFLDGGAIDI
EEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCEEEECCCEEEE
AMDYATVEKAGSRLGTGLAILLDDRTCPVGMLRNLEHFFAQESCGWCTPCRDGLPWVERL
EEHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHH
LDAIEEGRGEDGDLDLLAHHARMIGPMGRTFCAHAPGAIAPLSSGLNLFRAEFEAHLRLG
HHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHCHHHHHHHHHHHHHHC
RCPHRHAA
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7690854; 8157582; 9205837; 9278503; 8366049 [H]