Click here to switch to the map view.

The map label for this gene is tyrS [H]

Identifier: 162148158

GI number: 162148158

Start: 2449446

End: 2450687

Strand: Direct

Name: tyrS [H]

Synonym: GDI_2375

Alternate gene names: 162148158

Gene position: 2449446-2450687 (Clockwise)

Preceding gene: 162148157

Following gene: 162148159

Centisome position: 62.1

GC content: 67.95

Gene sequence:

>1242_bases
ATGCCTAAAAGCGAATTCCTCCAGGAAGCCGAAGCGCGCGGCTTCATCTTCCAGTGCACGGACATCGACGCCCTGGATGC
CGCCCTGCAGGCGGGGCCGGTGGCGGGCTATATCGGATTCGACCCCACGGCCGACAGCCTGCATGTCGGCAGCCTGATCC
AGATCATGATGCTGCGCCTGATGCAGAAGCACGGGCATCGGCCCGTGGCCCTGATGGGCGGGGGCACAGCGAAGATCGGC
GATCCCTCGTTCCGCGAGGAAGCCCGCCAGTTGATGACGGACGAGACGATCGCGGCGAACCTGCGCGGGATCGAGGGCTG
CCTGCGCCAGTTCCTGCAATTCGACGGCGCGGCCGGGGCCAGGCTGGCCAACAATGCCGACTGGCTGGACAGGCTGTCCT
ATATCGACCTGCTGCGCGACGTGGGCGTGCATTTTTCGGTCAACCGCATGCTGGCCTTCGATTCGGTCCGGTCGCGGCTG
GAGCGCGAACAGGGGCTGACCTTTCTGGAATTCAACTATTCCATCCTGCAATCCTACGATTTCCGTGAACTGAGCCGCAC
GCAGGGCGTCACCCTGCAGATGGGCGGATCGGACCAGTGGGGCAACATCGTATCGGGCATCGACCTGGTGCGCCGGACGG
ACCAGAAGCAGGTCTTCGGCCTGACGACGCCGCTGCTGACCACCGCGTCCGGGGCCAAGATGGGCAAGTCCGCGCGCGGC
GCGATCTGGCTGTCGGCGTCCCGGCTGCCGATCTTCGAATACTGGCAGTTCTGGCGCAACGTCGATGACGCGGATGTCGG
CCGGTTCCTGCGCCTGTTCACCGACCTGCCCATCGCGGAATGCGACCGGCTGGCGGCCCTGGGCGGCACCGAGATCAACG
AGGCGAAGAAGGTCCTGGCGACCGAGGCCACCGCCCTGTGCCACGGCCGCGCCGAAGCCGATGCCGCCGCCGAGGCCGCT
CGCCGCACGTTCGAGGACGGGGCCGCGCCCGCCGCCCTGCCCGGCACGACGATCGCCGCCGACCTGCTGGCGGCGGGCAT
TCCCGCCTTCCGCCTGTTCGTCGAAAGCGGCATGGCCGCCAGCAATGGCGAGGCGCGGCGCCTGATTCGCGGCGGCGGCG
CGCGGATCAACGACGTCGCGGTGCAGGATGAAGGCCAGGTGATCACCACGACGGACCTGGCGGACGGGGCCATCAAGCTG
TCGTCGGGACGCAAGCACCATATCCTGGTGCGGCCCGCGTGA

Upstream 100 bases:

>100_bases
TCATCTCGCAAGGCGGGCATGCTTCATCTCGCAAGGCGGGCATGCTAAGGCCTGTCGGGACATGTCCCCGACAGGCCGGC
CCGCCAGAGAGTACCCGACT

Downstream 100 bases:

>100_bases
TGACGCCCGCCTGATGGCTGCCGCGGCCGGGCCCGACGACGCCGCGCACCCGCCTGCGCCGCCCCCGCCCGTGCCGGACG
CGCTGTGGCAGCGCCTTGCG

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MPKSEFLQEAEARGFIFQCTDIDALDAALQAGPVAGYIGFDPTADSLHVGSLIQIMMLRLMQKHGHRPVALMGGGTAKIG
DPSFREEARQLMTDETIAANLRGIEGCLRQFLQFDGAAGARLANNADWLDRLSYIDLLRDVGVHFSVNRMLAFDSVRSRL
EREQGLTFLEFNYSILQSYDFRELSRTQGVTLQMGGSDQWGNIVSGIDLVRRTDQKQVFGLTTPLLTTASGAKMGKSARG
AIWLSASRLPIFEYWQFWRNVDDADVGRFLRLFTDLPIAECDRLAALGGTEINEAKKVLATEATALCHGRAEADAAAEAA
RRTFEDGAAPAALPGTTIAADLLAAGIPAFRLFVESGMAASNGEARRLIRGGGARINDVAVQDEGQVITTTDLADGAIKL
SSGRKHHILVRPA

Sequences:

>Translated_413_residues
MPKSEFLQEAEARGFIFQCTDIDALDAALQAGPVAGYIGFDPTADSLHVGSLIQIMMLRLMQKHGHRPVALMGGGTAKIG
DPSFREEARQLMTDETIAANLRGIEGCLRQFLQFDGAAGARLANNADWLDRLSYIDLLRDVGVHFSVNRMLAFDSVRSRL
EREQGLTFLEFNYSILQSYDFRELSRTQGVTLQMGGSDQWGNIVSGIDLVRRTDQKQVFGLTTPLLTTASGAKMGKSARG
AIWLSASRLPIFEYWQFWRNVDDADVGRFLRLFTDLPIAECDRLAALGGTEINEAKKVLATEATALCHGRAEADAAAEAA
RRTFEDGAAPAALPGTTIAADLLAAGIPAFRLFVESGMAASNGEARRLIRGGGARINDVAVQDEGQVITTTDLADGAIKL
SSGRKHHILVRPA
>Mature_412_residues
PKSEFLQEAEARGFIFQCTDIDALDAALQAGPVAGYIGFDPTADSLHVGSLIQIMMLRLMQKHGHRPVALMGGGTAKIGD
PSFREEARQLMTDETIAANLRGIEGCLRQFLQFDGAAGARLANNADWLDRLSYIDLLRDVGVHFSVNRMLAFDSVRSRLE
REQGLTFLEFNYSILQSYDFRELSRTQGVTLQMGGSDQWGNIVSGIDLVRRTDQKQVFGLTTPLLTTASGAKMGKSARGA
IWLSASRLPIFEYWQFWRNVDDADVGRFLRLFTDLPIAECDRLAALGGTEINEAKKVLATEATALCHGRAEADAAAEAAR
RTFEDGAAPAALPGTTIAADLLAAGIPAFRLFVESGMAASNGEARRLIRGGGARINDVAVQDEGQVITTTDLADGAIKLS
SGRKHHILVRPA

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=412, Percent_Identity=33.252427184466, Blast_Score=213, Evalue=3e-55,
Organism=Escherichia coli, GI1787925, Length=424, Percent_Identity=41.0377358490566, Blast_Score=307, Evalue=7e-85,
Organism=Caenorhabditis elegans, GI25145802, Length=339, Percent_Identity=32.4483775811209, Blast_Score=170, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI25145805, Length=232, Percent_Identity=31.4655172413793, Blast_Score=114, Evalue=9e-26,
Organism=Saccharomyces cerevisiae, GI6325160, Length=352, Percent_Identity=35.7954545454545, Blast_Score=211, Evalue=1e-55,
Organism=Drosophila melanogaster, GI20130383, Length=435, Percent_Identity=36.7816091954023, Blast_Score=253, Evalue=2e-67,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 44771; Mature: 44640

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS50889 S4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKSEFLQEAEARGFIFQCTDIDALDAALQAGPVAGYIGFDPTADSLHVGSLIQIMMLRL
CCHHHHHHHHHHCCEEEEECCCHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHH
MQKHGHRPVALMGGGTAKIGDPSFREEARQLMTDETIAANLRGIEGCLRQFLQFDGAAGA
HHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
RLANNADWLDRLSYIDLLRDVGVHFSVNRMLAFDSVRSRLEREQGLTFLEFNYSILQSYD
CCCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHCCEEEEECHHHHHCCC
FRELSRTQGVTLQMGGSDQWGNIVSGIDLVRRTDQKQVFGLTTPLLTTASGAKMGKSARG
HHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHCCCHHHHCCCHHHHHCCCCHHCCCCCCC
AIWLSASRLPIFEYWQFWRNVDDADVGRFLRLFTDLPIAECDRLAALGGTEINEAKKVLA
EEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHH
TEATALCHGRAEADAAAEAARRTFEDGAAPAALPGTTIAADLLAAGIPAFRLFVESGMAA
HHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCCC
SNGEARRLIRGGGARINDVAVQDEGQVITTTDLADGAIKLSSGRKHHILVRPA
CCCHHHHHHHCCCCEEEEEEEECCCCEEEEECCCCCEEEECCCCCEEEEEECC
>Mature Secondary Structure 
PKSEFLQEAEARGFIFQCTDIDALDAALQAGPVAGYIGFDPTADSLHVGSLIQIMMLRL
CHHHHHHHHHHCCEEEEECCCHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHH
MQKHGHRPVALMGGGTAKIGDPSFREEARQLMTDETIAANLRGIEGCLRQFLQFDGAAGA
HHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
RLANNADWLDRLSYIDLLRDVGVHFSVNRMLAFDSVRSRLEREQGLTFLEFNYSILQSYD
CCCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHCCEEEEECHHHHHCCC
FRELSRTQGVTLQMGGSDQWGNIVSGIDLVRRTDQKQVFGLTTPLLTTASGAKMGKSARG
HHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHCCCHHHHCCCHHHHHCCCCHHCCCCCCC
AIWLSASRLPIFEYWQFWRNVDDADVGRFLRLFTDLPIAECDRLAALGGTEINEAKKVLA
EEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHH
TEATALCHGRAEADAAAEAARRTFEDGAAPAALPGTTIAADLLAAGIPAFRLFVESGMAA
HHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCCC
SNGEARRLIRGGGARINDVAVQDEGQVITTTDLADGAIKLSSGRKHHILVRPA
CCCHHHHHHHCCCCEEEEEEEECCCCEEEEECCCCCEEEECCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA