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The map label for this gene is iscS [H]

Identifier: 162148154

GI number: 162148154

Start: 2445633

End: 2446859

Strand: Reverse

Name: iscS [H]

Synonym: GDI_2371

Alternate gene names: 162148154

Gene position: 2446859-2445633 (Counterclockwise)

Preceding gene: 162148155

Following gene: 162148153

Centisome position: 62.04

GC content: 72.37

Gene sequence:

>1227_bases
ATGACGGACGGGCGCGAACTCTATCTGGACTACCAGGCCACCACGCCGTGCGATCCGGCGGTCGTCGCGGCGATGATGCC
GTGGTTCGCCGAATCGTTCGGCAATCCCCACAGCGCCGATCACGCGCTGGGCCGCCGGGCCTGGGACGCGGGCGAGCAGG
CGCGGGCCGAGGTCGGCGCCCTGCTGGGTGCCGACGCGCGGGAAATCGTCTTCACCTCCGGGGCGACCGAGGTCAACAAC
ATCGCCATCAAGGGGGCGGTGCGCCATCTGGCGGCCCGGGGCGACGCCCGGCGGCGCGTCACTACCGTGGCCACGGAACA
TAAATGCGTGCTGGAATCGGTGCGCGACCTGGCGGCGGAAGGGTTCGATCCGGTGGTGCTGCCGGTCGCGCCCGATGGGC
GGCTGGACCCCGGGACGCTGCGCGCGGCGCTGGCGGTGCCCACCCTGCTGGTCAGCATCATGGCGGCGAACAACGAGACC
GGGGTGCTGCACGACATGGCCGCCCTGGCCCCCATTGTGCGGGCGGCGGGCGCGCTGCTGCACAGCGACGTGGCGCAGGC
GGCGGGCAAGGTGGCGCTGGATGTCCGGGCGATGGACCTGGACCTGGCATCGGTGTCGGCGCACAAGCTGTACGGGCCGA
AGGGGGTGGGCGCGCTGTATGTCCGGCGGCGGCCCCGGGTGCGGCTGGCGCCGCTGTTCTCGGGCGGCGGCCAGGAACGC
GGACTGCGCTCGGGCACGCTGCCCACGCCGCTGCTGGTGGGGTTCGGCGCCGCGTGCCGCATCGCGCGGACGGTCAGGGT
CGAGGAGGCACGGCGGATGGCGGCGCTGCGCGACGGATTCCTGGAGCGCATGGAGGAGGGCGTTCCCGGGATCGTGGTCA
ACGGGTCGATGGCGCACCGCCTGCCGGCCAACCTGAACCTGCGCTTTCCCGACGTGCGGGCGCTGGACGTGATCGCGCAT
GCGCCGGAACTGTGCGTATCCACCGGATCGGCGTGCTCGTCGGCCGAACTGGTGCCGTCCTATGTGCTGACGGCGATGGG
GCTGAGGGCCGAGGACGCGGCACGAAGCTTGCGTCTGGCGGTGGGACGCTATACCTCAGCCGCCGATATGGATCGTGCGG
CCGCGATCCTGTGCCGGGCTGTAGCGGATGCGCGCCGGGCTGAACGGGGGGCGGCCGCGCGCGCCGCCCACGACGATACG
GCCAGGACAGCAGAATGCCGCATATGA

Upstream 100 bases:

>100_bases
CGGTGCGCGAGGATGACGTGACGCGCTTCATCGCCGCCTGGCGTGCGATGGCGGCCCGGCTGCCGGCGCGTGCGAGATCG
GAACGAACGGAGGTCCACGT

Downstream 100 bases:

>100_bases
TTTTCATCGAGAGTGACGGAACGCGCCGCGAGGTCGACGCGCCGGTCGGTCTGTCGGTCCTCGAGATCGCGCACAAGCAC
GGGGTGGACCTGGAAGGCGC

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 408; Mature: 407

Protein sequence:

>408_residues
MTDGRELYLDYQATTPCDPAVVAAMMPWFAESFGNPHSADHALGRRAWDAGEQARAEVGALLGADAREIVFTSGATEVNN
IAIKGAVRHLAARGDARRRVTTVATEHKCVLESVRDLAAEGFDPVVLPVAPDGRLDPGTLRAALAVPTLLVSIMAANNET
GVLHDMAALAPIVRAAGALLHSDVAQAAGKVALDVRAMDLDLASVSAHKLYGPKGVGALYVRRRPRVRLAPLFSGGGQER
GLRSGTLPTPLLVGFGAACRIARTVRVEEARRMAALRDGFLERMEEGVPGIVVNGSMAHRLPANLNLRFPDVRALDVIAH
APELCVSTGSACSSAELVPSYVLTAMGLRAEDAARSLRLAVGRYTSAADMDRAAAILCRAVADARRAERGAAARAAHDDT
ARTAECRI

Sequences:

>Translated_408_residues
MTDGRELYLDYQATTPCDPAVVAAMMPWFAESFGNPHSADHALGRRAWDAGEQARAEVGALLGADAREIVFTSGATEVNN
IAIKGAVRHLAARGDARRRVTTVATEHKCVLESVRDLAAEGFDPVVLPVAPDGRLDPGTLRAALAVPTLLVSIMAANNET
GVLHDMAALAPIVRAAGALLHSDVAQAAGKVALDVRAMDLDLASVSAHKLYGPKGVGALYVRRRPRVRLAPLFSGGGQER
GLRSGTLPTPLLVGFGAACRIARTVRVEEARRMAALRDGFLERMEEGVPGIVVNGSMAHRLPANLNLRFPDVRALDVIAH
APELCVSTGSACSSAELVPSYVLTAMGLRAEDAARSLRLAVGRYTSAADMDRAAAILCRAVADARRAERGAAARAAHDDT
ARTAECRI
>Mature_407_residues
TDGRELYLDYQATTPCDPAVVAAMMPWFAESFGNPHSADHALGRRAWDAGEQARAEVGALLGADAREIVFTSGATEVNNI
AIKGAVRHLAARGDARRRVTTVATEHKCVLESVRDLAAEGFDPVVLPVAPDGRLDPGTLRAALAVPTLLVSIMAANNETG
VLHDMAALAPIVRAAGALLHSDVAQAAGKVALDVRAMDLDLASVSAHKLYGPKGVGALYVRRRPRVRLAPLFSGGGQERG
LRSGTLPTPLLVGFGAACRIARTVRVEEARRMAALRDGFLERMEEGVPGIVVNGSMAHRLPANLNLRFPDVRALDVIAHA
PELCVSTGSACSSAELVPSYVLTAMGLRAEDAARSLRLAVGRYTSAADMDRAAAILCRAVADARRAERGAAARAAHDDTA
RTAECRI

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=367, Percent_Identity=48.2288828337875, Blast_Score=358, Evalue=6e-99,
Organism=Homo sapiens, GI156713448, Length=414, Percent_Identity=34.2995169082126, Blast_Score=195, Evalue=8e-50,
Organism=Escherichia coli, GI48994898, Length=381, Percent_Identity=44.0944881889764, Blast_Score=319, Evalue=2e-88,
Organism=Escherichia coli, GI1789175, Length=232, Percent_Identity=31.8965517241379, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787970, Length=232, Percent_Identity=29.7413793103448, Blast_Score=86, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI25143064, Length=373, Percent_Identity=45.0402144772118, Blast_Score=328, Evalue=3e-90,
Organism=Caenorhabditis elegans, GI17533177, Length=322, Percent_Identity=32.9192546583851, Blast_Score=152, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6319831, Length=379, Percent_Identity=43.5356200527704, Blast_Score=318, Evalue=1e-87,
Organism=Drosophila melanogaster, GI20129463, Length=374, Percent_Identity=48.1283422459893, Blast_Score=349, Evalue=2e-96,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 43021; Mature: 42890

Theoretical pI: Translated: 8.30; Mature: 8.30

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDGRELYLDYQATTPCDPAVVAAMMPWFAESFGNPHSADHALGRRAWDAGEQARAEVGA
CCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHH
LLGADAREIVFTSGATEVNNIAIKGAVRHLAARGDARRRVTTVATEHKCVLESVRDLAAE
HHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHC
GFDPVVLPVAPDGRLDPGTLRAALAVPTLLVSIMAANNETGVLHDMAALAPIVRAAGALL
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
HSDVAQAAGKVALDVRAMDLDLASVSAHKLYGPKGVGALYVRRRPRVRLAPLFSGGGQER
HHHHHHHCCCEEEEEEEEECCHHHHHHHHCCCCCCCCEEEEECCCCEEEEEEECCCCCCC
GLRSGTLPTPLLVGFGAACRIARTVRVEEARRMAALRDGFLERMEEGVPGIVVNGSMAHR
CCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHC
LPANLNLRFPDVRALDVIAHAPELCVSTGSACSSAELVPSYVLTAMGLRAEDAARSLRLA
CCCCCEEECCCCHHHHHHHHCHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHH
VGRYTSAADMDRAAAILCRAVADARRAERGAAARAAHDDTARTAECRI
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TDGRELYLDYQATTPCDPAVVAAMMPWFAESFGNPHSADHALGRRAWDAGEQARAEVGA
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHH
LLGADAREIVFTSGATEVNNIAIKGAVRHLAARGDARRRVTTVATEHKCVLESVRDLAAE
HHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHC
GFDPVVLPVAPDGRLDPGTLRAALAVPTLLVSIMAANNETGVLHDMAALAPIVRAAGALL
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
HSDVAQAAGKVALDVRAMDLDLASVSAHKLYGPKGVGALYVRRRPRVRLAPLFSGGGQER
HHHHHHHCCCEEEEEEEEECCHHHHHHHHCCCCCCCCEEEEECCCCEEEEEEECCCCCCC
GLRSGTLPTPLLVGFGAACRIARTVRVEEARRMAALRDGFLERMEEGVPGIVVNGSMAHR
CCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHC
LPANLNLRFPDVRALDVIAHAPELCVSTGSACSSAELVPSYVLTAMGLRAEDAARSLRLA
CCCCCEEECCCCHHHHHHHHCHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHH
VGRYTSAADMDRAAAILCRAVADARRAERGAAARAAHDDTARTAECRI
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA