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The map label for this gene is ubiH [H]

Identifier: 162148133

GI number: 162148133

Start: 2421686

End: 2422948

Strand: Reverse

Name: ubiH [H]

Synonym: GDI_2350

Alternate gene names: 162148133

Gene position: 2422948-2421686 (Counterclockwise)

Preceding gene: 162148137

Following gene: 162148132

Centisome position: 61.43

GC content: 70.78

Gene sequence:

>1263_bases
ATGACCGCAGGCACGCAGCCCTTTCCCCGGACCGGATTTGCCGATGCCCGTGACGTCCCGCCTGACATCCCCGGTGATGT
CGATGTCTGCATCGTGGGCGCGGGGCCGGTCGGCGCCACGCTGGCCTGCCGCTTGGCGATCGGGGGGCTGTCCGTGGCCA
TTGTCGACCGCGCACCCCTCTCGCCCATGGAAGATCCGGCCTTCGACGGCCGCGCATACGCCATCGCTGCCGGATCGCGC
CGGCTGCTGGATGCGGCCGGGGTATGGGACCGCCTGCCGCTGCCGGCCTGCCCGATCGAGGAAATCCGCGTCTCCGACGG
ACGCCCGGGCGAGGCCCCGTCGCCCCTGTTCCTGGAATTCGGACGGCAGGACGCCGACCAGCCCTTCGGCTGGATGATCG
AGGCCCGGGCGTTGCGCGTGGCGCTGAACGCGTCACTGAGTGCCCATGAGACGATCACCGTGCTGGCGCCGGAGGAAGTC
ACCGTGACACGCACGGAATCGGGTGCGCGAATCCGCACCCGCGCGGGCCACGAATTCGGCGCGCGGCTTGTGGTCGCGGC
GGAAGGGCGGCGCAGCACCCTGCGCGACCAGGCCCGCATCGCCGTCACGCGCGTGCCGTACCATACCAGCGGCATCGTCT
GCAGCGTGGCGCATGAACGCCCGCACCAGAATCGGGCGCTGGAACATTTCCTGCCCGCCGGTCCGTTCGCGCAACTGCCG
ATGGCCCCCACCCCGGATTACCCGAACCTGTCGGCCATCGTCTGGTCGGAAAGCGATGCCGTGGCCCAGCGTATGGTCGC
CCTGCCGCCTGATGCCTTCGCGCACGAAATCCAGCGCCGGATGGGCGACTGGCTGGGCCGCGTGACCTCGGTCGGGCAGC
GCTGGACCTATCCGCTGTCGCTTCAGTATGCCCAGCGCTATTTCGATACGCGGCTGGCGCTGGCGGGCGACGCCGCCCAC
GGCATGCACCCGATCGCGGGGCAGGGCCTGAATGTGGGCTTCCGCGACGTCATCGCGCTGTCCGACCTGCTGATCGCGGC
CCATGCGCGGGGCGAGGACCCGGGCAGCCCGACGCTGCTGCGCCGCTACCAGGCCCGCTGCCGCCCGTCCAACATGGTGA
TGCTGGCCGCGACCGACATGCTGGAACGCCTGTTCGGCAACGACAATCCCATCCTGCGCGGCGTCCGCGACCTGGGCCTG
GCGGGGGTGCATCGCCTGCCCCGGCTGCGCCGGGCCTTCGTGAAACAGGCCATGGGGCTATAG

Upstream 100 bases:

>100_bases
GACCCGGGGGATTCTGCCTGATTTATGGGCTGGCAGCACCGGAACGGATCACGATTGTCGCGGGTCGTCCGATCCGCATG
GCCACCACAGGACCCCACGC

Downstream 100 bases:

>100_bases
CTCTTCCGCACCTCCCTCTGGTCAGGGCGGGCGCGGATTACCATATCGTCATTCTCGTCCATTCATCAGGTTCACCATGC
CCGATACGTCCCTGATCTCG

Product: 2-octaprenyl-6-methoxyphenol hydroxylase

Products: H2O; 2-octaprenyl-6-methoxy-1,4-benzoquinone [C]

Alternate protein names: NA

Number of amino acids: Translated: 420; Mature: 419

Protein sequence:

>420_residues
MTAGTQPFPRTGFADARDVPPDIPGDVDVCIVGAGPVGATLACRLAIGGLSVAIVDRAPLSPMEDPAFDGRAYAIAAGSR
RLLDAAGVWDRLPLPACPIEEIRVSDGRPGEAPSPLFLEFGRQDADQPFGWMIEARALRVALNASLSAHETITVLAPEEV
TVTRTESGARIRTRAGHEFGARLVVAAEGRRSTLRDQARIAVTRVPYHTSGIVCSVAHERPHQNRALEHFLPAGPFAQLP
MAPTPDYPNLSAIVWSESDAVAQRMVALPPDAFAHEIQRRMGDWLGRVTSVGQRWTYPLSLQYAQRYFDTRLALAGDAAH
GMHPIAGQGLNVGFRDVIALSDLLIAAHARGEDPGSPTLLRRYQARCRPSNMVMLAATDMLERLFGNDNPILRGVRDLGL
AGVHRLPRLRRAFVKQAMGL

Sequences:

>Translated_420_residues
MTAGTQPFPRTGFADARDVPPDIPGDVDVCIVGAGPVGATLACRLAIGGLSVAIVDRAPLSPMEDPAFDGRAYAIAAGSR
RLLDAAGVWDRLPLPACPIEEIRVSDGRPGEAPSPLFLEFGRQDADQPFGWMIEARALRVALNASLSAHETITVLAPEEV
TVTRTESGARIRTRAGHEFGARLVVAAEGRRSTLRDQARIAVTRVPYHTSGIVCSVAHERPHQNRALEHFLPAGPFAQLP
MAPTPDYPNLSAIVWSESDAVAQRMVALPPDAFAHEIQRRMGDWLGRVTSVGQRWTYPLSLQYAQRYFDTRLALAGDAAH
GMHPIAGQGLNVGFRDVIALSDLLIAAHARGEDPGSPTLLRRYQARCRPSNMVMLAATDMLERLFGNDNPILRGVRDLGL
AGVHRLPRLRRAFVKQAMGL
>Mature_419_residues
TAGTQPFPRTGFADARDVPPDIPGDVDVCIVGAGPVGATLACRLAIGGLSVAIVDRAPLSPMEDPAFDGRAYAIAAGSRR
LLDAAGVWDRLPLPACPIEEIRVSDGRPGEAPSPLFLEFGRQDADQPFGWMIEARALRVALNASLSAHETITVLAPEEVT
VTRTESGARIRTRAGHEFGARLVVAAEGRRSTLRDQARIAVTRVPYHTSGIVCSVAHERPHQNRALEHFLPAGPFAQLPM
APTPDYPNLSAIVWSESDAVAQRMVALPPDAFAHEIQRRMGDWLGRVTSVGQRWTYPLSLQYAQRYFDTRLALAGDAAHG
MHPIAGQGLNVGFRDVIALSDLLIAAHARGEDPGSPTLLRRYQARCRPSNMVMLAATDMLERLFGNDNPILRGVRDLGLA
GVHRLPRLRRAFVKQAMGL

Specific function: Oxygenase that introduces the hydroxyl group at carbon four of 2-octaprenyl-6-methoxyphenol resulting in the formation of 2-octaprenyl-6-methoxy-1,4-benzoquinone [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=424, Percent_Identity=26.6509433962264, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI32967307, Length=346, Percent_Identity=28.0346820809249, Blast_Score=116, Evalue=3e-26,
Organism=Escherichia coli, GI1789274, Length=400, Percent_Identity=33.5, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI1789273, Length=400, Percent_Identity=35.25, Blast_Score=188, Evalue=4e-49,
Organism=Escherichia coli, GI1786883, Length=397, Percent_Identity=33.2493702770781, Blast_Score=187, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI25146631, Length=429, Percent_Identity=27.972027972028, Blast_Score=144, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6321694, Length=482, Percent_Identity=26.9709543568465, Blast_Score=113, Evalue=6e-26,
Organism=Drosophila melanogaster, GI19920760, Length=428, Percent_Identity=25.4672897196262, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971
- InterPro:   IPR011295 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 45461; Mature: 45329

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: PS01304 UBIH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAGTQPFPRTGFADARDVPPDIPGDVDVCIVGAGPVGATLACRLAIGGLSVAIVDRAPL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHEECCCEEEEEEECCCC
SPMEDPAFDGRAYAIAAGSRRLLDAAGVWDRLPLPACPIEEIRVSDGRPGEAPSPLFLEF
CCCCCCCCCCCEEEEECCCCHHHHHCCCHHCCCCCCCCHHHHCCCCCCCCCCCCCHHEEC
GRQDADQPFGWMIEARALRVALNASLSAHETITVLAPEEVTVTRTESGARIRTRAGHEFG
CCCCCCCCCCEEEEHHEEEEEECCCCCCCCEEEEECCCCEEEEECCCCCEEEECCCCCCC
ARLVVAAEGRRSTLRDQARIAVTRVPYHTSGIVCSVAHERPHQNRALEHFLPAGPFAQLP
CEEEEEECCCHHHHHHHHEEEEEECCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCC
MAPTPDYPNLSAIVWSESDAVAQRMVALPPDAFAHEIQRRMGDWLGRVTSVGQRWTYPLS
CCCCCCCCCCEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
LQYAQRYFDTRLALAGDAAHGMHPIAGQGLNVGFRDVIALSDLLIAAHARGEDPGSPTLL
HHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHH
RRYQARCRPSNMVMLAATDMLERLFGNDNPILRGVRDLGLAGVHRLPRLRRAFVKQAMGL
HHHHHHCCCCCEEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TAGTQPFPRTGFADARDVPPDIPGDVDVCIVGAGPVGATLACRLAIGGLSVAIVDRAPL
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHEECCCEEEEEEECCCC
SPMEDPAFDGRAYAIAAGSRRLLDAAGVWDRLPLPACPIEEIRVSDGRPGEAPSPLFLEF
CCCCCCCCCCCEEEEECCCCHHHHHCCCHHCCCCCCCCHHHHCCCCCCCCCCCCCHHEEC
GRQDADQPFGWMIEARALRVALNASLSAHETITVLAPEEVTVTRTESGARIRTRAGHEFG
CCCCCCCCCCEEEEHHEEEEEECCCCCCCCEEEEECCCCEEEEECCCCCEEEECCCCCCC
ARLVVAAEGRRSTLRDQARIAVTRVPYHTSGIVCSVAHERPHQNRALEHFLPAGPFAQLP
CEEEEEECCCHHHHHHHHEEEEEECCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCC
MAPTPDYPNLSAIVWSESDAVAQRMVALPPDAFAHEIQRRMGDWLGRVTSVGQRWTYPLS
CCCCCCCCCCEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
LQYAQRYFDTRLALAGDAAHGMHPIAGQGLNVGFRDVIALSDLLIAAHARGEDPGSPTLL
HHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHH
RRYQARCRPSNMVMLAATDMLERLFGNDNPILRGVRDLGLAGVHRLPRLRRAFVKQAMGL
HHHHHHCCCCCEEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-octaprenyl-6-methoxyphenol; O2 [C]

Specific reaction: 2-octaprenyl-6-methoxyphenol + O2 = H2O + 2-octaprenyl-6-methoxy-1,4-benzoquinone [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1339425; 9278503 [H]