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The map label for this gene is recA [H]

Identifier: 162148079

GI number: 162148079

Start: 2357205

End: 2358350

Strand: Reverse

Name: recA [H]

Synonym: GDI_2296

Alternate gene names: 162148079

Gene position: 2358350-2357205 (Counterclockwise)

Preceding gene: 162148080

Following gene: 162148078

Centisome position: 59.79

GC content: 64.66

Gene sequence:

>1146_bases
ATGCGGCCCCGGTGCCGATCCTGGAGGCGAGCCCAGGCGGGCGGAGCGGGCAGGACCTGGGTATTTCATGATAGTGGAGC
GGGGTTAAGGGCGATGGCGCAGGCACCGGGCATTGACAAGAGCAAGGCGCTGGAAGGCGCGCTCAGCCAGATCGAGCGGG
CATTCGGCAAGGGGTCCGTGATGCGCCTGGGCCAGCGCGCCAACGAGGCGGTGGATGTCATTTCCACCGGCTCGCTGGGT
CTGGATATCGCGCTGGGAATCGGCGGCCTGCCGCGTGGCCGTATCGTCGAGATCTACGGTCCCGAAAGCTCGGGCAAGAC
GACGCTGGCGCTGCATGCGATCGCCGAGGCCCAGAAGAAGGGCGGCGTGTGCGCCTTCATCGACGCCGAGCACGCGCTGG
ACCCCGGCTATGCCCGCAAGCTGGGGGTCGATGTCGACAACCTGCTGATCAGCCAGCCCGACGCGGGCGAGCAGGCGCTG
GAAATCGCGGATACGCTGGTGCGGTCGGGCGCGATCGACGTGCTGGTGGTGGACAGCGTCGCGGCCCTGGTGCCCCGGGC
GGAACTGGAAGGCGACATGGGCGACAGCCATGTCGGCCTGCATGCCCGGCTGATGAGCCAGGCGCTGCGCAAGCTGACGG
GCACGGTGTCGCGCTCCAACACCATGATGATCTTCCTGAACCAGATCCGGTTGAAGATCGGCGTGATGTTCGGCAGCCCC
GAGACGACGACGGGCGGCAACGCGCTGAAGTTCTATGCCTCGGTGCGGATGGATATCCGCCGGATCGGGCAGATCAAGGA
CAAGGACGAGGTCGTGGGCAACCAGACCCGCGTGAAGGTCGTCAAGAACAAGATGGCCCCCCCCTTCCGCCAGGTCGAAT
TCGACATCGTGTATGGCGAAGGGATCAGCAAGATGGGCGAACTGATCGATCTGGGCGTCAAGGCAGGCATTGTCGAGAAA
TCGGGGGCCTGGTTTTCCTATGACAGCCAGCGGATCGGGCAGGGGCGGGAGAATTCCAAGCAATATCTGCGCGAACATCC
CGAAATGGCGGCCGATATCGAACGGCGGGTCCGCGAACAGGCTGGCGTAGTGGCCGAAGCGATGCTGGTATCCCCCGGTG
AACAGGATCTTGCCGAAATCGAATAA

Upstream 100 bases:

>100_bases
CGGGGCTGGCGGTCGGGTCGGATTAAACCCCCGGCCGGCAAGGGGGTCGCTTGTGGGCGATCATGTTTTGTTCTAGTCTT
GGCGAACAGGGATTCGCGTC

Downstream 100 bases:

>100_bases
CCGAAACGGCCCGCCTTTCGGTGTGATGCTGATGCTGCTGGCCGGCCTGGCGGCGTGTACGGTGAAGCGGCCGGAAGGGG
ATTCGATCGACGTTCCGCCG

Product: protein recA (recombinase A)

Products: NA

Alternate protein names: Recombinase A [H]

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MRPRCRSWRRAQAGGAGRTWVFHDSGAGLRAMAQAPGIDKSKALEGALSQIERAFGKGSVMRLGQRANEAVDVISTGSLG
LDIALGIGGLPRGRIVEIYGPESSGKTTLALHAIAEAQKKGGVCAFIDAEHALDPGYARKLGVDVDNLLISQPDAGEQAL
EIADTLVRSGAIDVLVVDSVAALVPRAELEGDMGDSHVGLHARLMSQALRKLTGTVSRSNTMMIFLNQIRLKIGVMFGSP
ETTTGGNALKFYASVRMDIRRIGQIKDKDEVVGNQTRVKVVKNKMAPPFRQVEFDIVYGEGISKMGELIDLGVKAGIVEK
SGAWFSYDSQRIGQGRENSKQYLREHPEMAADIERRVREQAGVVAEAMLVSPGEQDLAEIE

Sequences:

>Translated_381_residues
MRPRCRSWRRAQAGGAGRTWVFHDSGAGLRAMAQAPGIDKSKALEGALSQIERAFGKGSVMRLGQRANEAVDVISTGSLG
LDIALGIGGLPRGRIVEIYGPESSGKTTLALHAIAEAQKKGGVCAFIDAEHALDPGYARKLGVDVDNLLISQPDAGEQAL
EIADTLVRSGAIDVLVVDSVAALVPRAELEGDMGDSHVGLHARLMSQALRKLTGTVSRSNTMMIFLNQIRLKIGVMFGSP
ETTTGGNALKFYASVRMDIRRIGQIKDKDEVVGNQTRVKVVKNKMAPPFRQVEFDIVYGEGISKMGELIDLGVKAGIVEK
SGAWFSYDSQRIGQGRENSKQYLREHPEMAADIERRVREQAGVVAEAMLVSPGEQDLAEIE
>Mature_381_residues
MRPRCRSWRRAQAGGAGRTWVFHDSGAGLRAMAQAPGIDKSKALEGALSQIERAFGKGSVMRLGQRANEAVDVISTGSLG
LDIALGIGGLPRGRIVEIYGPESSGKTTLALHAIAEAQKKGGVCAFIDAEHALDPGYARKLGVDVDNLLISQPDAGEQAL
EIADTLVRSGAIDVLVVDSVAALVPRAELEGDMGDSHVGLHARLMSQALRKLTGTVSRSNTMMIFLNQIRLKIGVMFGSP
ETTTGGNALKFYASVRMDIRRIGQIKDKDEVVGNQTRVKVVKNKMAPPFRQVEFDIVYGEGISKMGELIDLGVKAGIVEK
SGAWFSYDSQRIGQGRENSKQYLREHPEMAADIERRVREQAGVVAEAMLVSPGEQDLAEIE

Specific function: Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with lexA causing its activation

COG id: COG0468

COG function: function code L; RecA/RadA recombinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family [H]

Homologues:

Organism=Escherichia coli, GI1789051, Length=321, Percent_Identity=69.4704049844237, Blast_Score=475, Evalue=1e-135,

Paralogues:

None

Copy number: 1548 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800-1200 (L-broth) 40,000-60,000 (L-broth + Nalidixate) 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013765
- InterPro:   IPR020584
- InterPro:   IPR020588
- InterPro:   IPR020587 [H]

Pfam domain/function: PF00154 RecA [H]

EC number: NA

Molecular weight: Translated: 41020; Mature: 41020

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: PS00321 RECA_1 ; PS50162 RECA_2 ; PS50163 RECA_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPRCRSWRRAQAGGAGRTWVFHDSGAGLRAMAQAPGIDKSKALEGALSQIERAFGKGSV
CCCCCHHHHHHCCCCCCCEEEEECCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHH
MRLGQRANEAVDVISTGSLGLDIALGIGGLPRGRIVEIYGPESSGKTTLALHAIAEAQKK
HHHHHHHHHHHHEEECCCCCEEEEECCCCCCCCCEEEEECCCCCCCEEEEHHHHHHHHHC
GGVCAFIDAEHALDPGYARKLGVDVDNLLISQPDAGEQALEIADTLVRSGAIDVLVVDSV
CCEEEEEECHHCCCCCHHHHHCCCHHHHEECCCCCCHHHHHHHHHHHHCCCCEEEEHHHH
AALVPRAELEGDMGDSHVGLHARLMSQALRKLTGTVSRSNTMMIFLNQIRLKIGVMFGSP
HHHCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEEEEEEEECCC
ETTTGGNALKFYASVRMDIRRIGQIKDKDEVVGNQTRVKVVKNKMAPPFRQVEFDIVYGE
CCCCCCCCEEEHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHCCCCHHHEEEEEEECC
GISKMGELIDLGVKAGIVEKSGAWFSYDSQRIGQGRENSKQYLREHPEMAADIERRVREQ
CHHHHHHHHHHHHHCCEEECCCCEEECCHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHH
AGVVAEAMLVSPGEQDLAEIE
HCHHHHHHHCCCCHHHHHHCC
>Mature Secondary Structure
MRPRCRSWRRAQAGGAGRTWVFHDSGAGLRAMAQAPGIDKSKALEGALSQIERAFGKGSV
CCCCCHHHHHHCCCCCCCEEEEECCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHH
MRLGQRANEAVDVISTGSLGLDIALGIGGLPRGRIVEIYGPESSGKTTLALHAIAEAQKK
HHHHHHHHHHHHEEECCCCCEEEEECCCCCCCCCEEEEECCCCCCCEEEEHHHHHHHHHC
GGVCAFIDAEHALDPGYARKLGVDVDNLLISQPDAGEQALEIADTLVRSGAIDVLVVDSV
CCEEEEEECHHCCCCCHHHHHCCCHHHHEECCCCCCHHHHHHHHHHHHCCCCEEEEHHHH
AALVPRAELEGDMGDSHVGLHARLMSQALRKLTGTVSRSNTMMIFLNQIRLKIGVMFGSP
HHHCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEEEEEEEECCC
ETTTGGNALKFYASVRMDIRRIGQIKDKDEVVGNQTRVKVVKNKMAPPFRQVEFDIVYGE
CCCCCCCCEEEHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHCCCCHHHEEEEEEECC
GISKMGELIDLGVKAGIVEKSGAWFSYDSQRIGQGRENSKQYLREHPEMAADIERRVREQ
CHHHHHHHHHHHHHCCEEECCCCEEECCHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHH
AGVVAEAMLVSPGEQDLAEIE
HCHHHHHHHCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8486287 [H]