Click here to switch to the map view.

The map label for this gene is mcbR [H]

Identifier: 162148069

GI number: 162148069

Start: 2344954

End: 2345610

Strand: Reverse

Name: mcbR [H]

Synonym: GDI_2286

Alternate gene names: 162148069

Gene position: 2345610-2344954 (Counterclockwise)

Preceding gene: 162148070

Following gene: 162148064

Centisome position: 59.47

GC content: 68.95

Gene sequence:

>657_bases
ATGACGAGCGACGGCGGGCGCAAGATGCTGGGCATCGACGCCTACAATCATATCCGCACCGGGCTGATCCGCAGCCGCTA
CCGCGTGGGTCAGCGGCTGGTGCTGCGTCCCCTGGCGGCCGAACTGGGCCTGAGTCCCACCCCGGTCCGCGAAGCGCTGC
TGCGTCTCGTGTCCGAACAGGCGCTGGAACTGAACGATCGCAATACGGCGGTGGTGCCCGAGATCACGCCCGCCAGCTTT
CGGGAAATCCATACGCTGCGGGTCGATCTGGAAAGCCGGCTGGTCCAGGCGGCGGCGCTGCGCGCCACGTCCGCCGAACT
GGACGCGCTTGCCGAACTGGATGACGATTTCCAGGAGGCCTGCACCCGTCACGACGATGCCCGGATGGCGGCCAGCAACG
CCGATTTCCACGCCATGCTGGCCGCGATCGCGGACCTGCCGCTGACGGCGAATATCCTGCGCAGCCTGTGGGCCCGGATC
GGCCCGCTCTACGCGCTGGCACGGACCGGTCCGCCCACCCGGCCGCCCGGTGTCCCGCACCCGCATGACGAATTGCTGCG
CGCATTGCGCGCCCGCGACCCCATCGCGGCCAGCCTCGCCCTGGTCCATGACGCGGAATATGCGCGCACCTGGATCGAGC
CCCTGATGCCTTTCTAG

Upstream 100 bases:

>100_bases
CGTGCAGGACGGCCCCGTCGCGCTGCTGGAGCGCAGCGTGGGCCCGTATCGCGATTTCCTGGAGCGTCAGTCGATGACCG
TGGAAGGCGCGGTATAAGGC

Downstream 100 bases:

>100_bases
GAGGCGGCTTCAAAAGTCTCCGAGCCTGGGGGCGCCTATTTGATGGCGGCCGCCGCCTGCGGCGCCGCGCGGGCCTTCAG
CTCCGGGACCTCGATGTCGA

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MTSDGGRKMLGIDAYNHIRTGLIRSRYRVGQRLVLRPLAAELGLSPTPVREALLRLVSEQALELNDRNTAVVPEITPASF
REIHTLRVDLESRLVQAAALRATSAELDALAELDDDFQEACTRHDDARMAASNADFHAMLAAIADLPLTANILRSLWARI
GPLYALARTGPPTRPPGVPHPHDELLRALRARDPIAASLALVHDAEYARTWIEPLMPF

Sequences:

>Translated_218_residues
MTSDGGRKMLGIDAYNHIRTGLIRSRYRVGQRLVLRPLAAELGLSPTPVREALLRLVSEQALELNDRNTAVVPEITPASF
REIHTLRVDLESRLVQAAALRATSAELDALAELDDDFQEACTRHDDARMAASNADFHAMLAAIADLPLTANILRSLWARI
GPLYALARTGPPTRPPGVPHPHDELLRALRARDPIAASLALVHDAEYARTWIEPLMPF
>Mature_217_residues
TSDGGRKMLGIDAYNHIRTGLIRSRYRVGQRLVLRPLAAELGLSPTPVREALLRLVSEQALELNDRNTAVVPEITPASFR
EIHTLRVDLESRLVQAAALRATSAELDALAELDDDFQEACTRHDDARMAASNADFHAMLAAIADLPLTANILRSLWARIG
PLYALARTGPPTRPPGVPHPHDELLRALRARDPIAASLALVHDAEYARTWIEPLMPF

Specific function: Important for biofilm formation. Represses expression of mcbA by binding to its promoter region, which prevents colanic acid overproduction and mucoidy [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081914, Length=185, Percent_Identity=32.4324324324324, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 24028; Mature: 23897

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSDGGRKMLGIDAYNHIRTGLIRSRYRVGQRLVLRPLAAELGLSPTPVREALLRLVSEQ
CCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
ALELNDRNTAVVPEITPASFREIHTLRVDLESRLVQAAALRATSAELDALAELDDDFQEA
HHHCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
CTRHDDARMAASNADFHAMLAAIADLPLTANILRSLWARIGPLYALARTGPPTRPPGVPH
HHCCHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
PHDELLRALRARDPIAASLALVHDAEYARTWIEPLMPF
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TSDGGRKMLGIDAYNHIRTGLIRSRYRVGQRLVLRPLAAELGLSPTPVREALLRLVSEQ
CCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
ALELNDRNTAVVPEITPASFREIHTLRVDLESRLVQAAALRATSAELDALAELDDDFQEA
HHHCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
CTRHDDARMAASNADFHAMLAAIADLPLTANILRSLWARIGPLYALARTGPPTRPPGVPH
HHCCHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
PHDELLRALRARDPIAASLALVHDAEYARTWIEPLMPF
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]