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The map label for this gene is aspS [H]

Identifier: 162148016

GI number: 162148016

Start: 2287057

End: 2288898

Strand: Reverse

Name: aspS [H]

Synonym: GDI_2232

Alternate gene names: 162148016

Gene position: 2288898-2287057 (Counterclockwise)

Preceding gene: 162148017

Following gene: 162148015

Centisome position: 58.03

GC content: 67.97

Gene sequence:

>1842_bases
ATGGCGTCCCGTCCCCTGCCTTTGGTGACACAGGACCAGACCATGCATCCCTATCGTACCCATAGCTGCGCGGCCCTTCG
CGCCAGCGATGCCGGGCAGACGGCCCGCCTCTCCGGCTGGGTGCACAGCAAGCGCGATCATGGCGGCCTGCTGTTCATCG
ACCTGCGCGATCATTTCGGCATGACCCAGATCGTCATTCCCGCCGGTTCCCCGGTGCTGGAGGCCGTCGAGCGCGTTCGC
GTGGAAAGCGTGCTGACCATCACGGGCGAAGTCGTGCTGCGCGACGCGGCGACGAAGAACCCGAACCTGCCGACCGGCGA
GATCGAACTGCGCGCGCAGCAGGTCGACATCCAGTCCTCGGCCGACGTGCTGCCCTTCCAGGTCGCGGGGCACGAGCATT
ACCCCGAGGATCTGCGCCTGACCTACCGCTACATCGACCTGCGGCGCGACAAGGTCCATCGCAACATGATGCTGCGCGCC
CAGGTGATCGCCAGCCTGCGCCGCCGCATGACCGACCAGGGCTTCGTCGAATTCCAGACCCCGATCCTGACCGCGTCCTC
GCCCGAGGGCGCGCGGGACTTCCTGGTGCCGGCCCGCATGCATCCGGGCAAGTTCTATGCGCTGCCCCAGGCGCCGCAGC
AGTTCAAGCAACTGGCGATGGTCGCGGGCTTCGACCGCTATTTCCAGATCGCCCCGTGCTTCCGGGACGAGGCCGCGCGC
GCCGACCGCTCGCCGGGCGAATTCTACCAGCTCGACTTCGAAATGGCGTTCGCGACCCAGGAAGACGTGTTCGCGACCCT
GGAGCCGGTGATGGAAGGCGTGTTCCGCGAATTCGGCGGCGGCCGCACGGTCAGCGCGGCCCCCTTCGAGCGGATTCCCT
ACGCCACGGCGATGAAGCTGTACGGCAGCGACAAGCCCGACCTGCGCAACCCCCTGCTGCTGAGCGACGTGACCGAATCG
TTCGCCAACTCCGGCTTCGGCCTGTTCGCGCGCATCGTCGCCGACGGGGGCGAGGTCCGCGCGATTCCGGCGCCGGGGGC
GGGCGATCGTCCGCGCGCCTTCTATGACAAGCTGAATGCCTGGGCGCGCGAAGCCGGGGCCGGCGGCCTGGGCTACATCA
TCTTCGACGAAGAGGGTGGCAAGGGCCCGATCGCCAAGAACCTGGAGCCCGCGCGCGTCGCCGCGATTCGCGAGGCCACA
GGCCTGAAGGCCGGTGACGCGGTCTTCTTCGCGGCCGGCAAGGGCGACGAGGTCGTGAAATTCTCCGGCCTGGTGCGCAC
GCGCATCGCGACCGAGCTGGACCTGATCGAACAGGACTCCTTCCGCTTCTGCTGGATCACCGATTTCCCGATGTACGAGC
GCAACGAGGAAACCGGGCAGATCGACTTCTCGCACAACCCGTTCTCGATGCCCCAGGGCGGGCTGGAGGCGCTGAACGGC
CAGGACCCGCTGACGATCACGGCCTATCAGTACGATATCGTGTGCAACGGCGTCGAACTGTCCTCGGGCGCCATCCGCAA
CCATCGCCCCGACGTGATGATCCGTGCGTTCGAACTGGCCGGCTACCCGGCCAGCGAGGTCGAGGCCCGTTTCGGCGGCA
TGCTCAACGCCTTCCGCTACGGCGCGCCGCCGCATGGCGGCTCGGCACCGGGCGTGGACCGCATCGTCATGCTGCTGGCC
GACGAGCCGAACATCCGCGAGGTCATCCTCTTCCCGCTGAACCAGCAGGGCGAGGACCTGATGATGGGCGCGCCGGCCCC
CGTGCCCGCCGAACGGCTGAAGGAACTGTCGCTGGCGCTGAACCTGCCGCGGCCGAAGCCCGGCGTGAAGGCCGGGGAAT
AA

Upstream 100 bases:

>100_bases
CCGAACACGCATGACGCCACGCGGCCGGCCGACCCGTCTCGGCAACCCTTGACAGGGGCGGCCCGGCCCGGCTTCCTCCG
CCTTCGATCGCGCGGGGCGC

Downstream 100 bases:

>100_bases
GGACCGCGCGTCATTGTGCGATTGCGCGGCGTATCGCGATGGGGTCATGCTGGCGCCATGACATGGTGGCCTCTTCTTCG
CGGGATCGTGGCGCGCGCCG

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 613; Mature: 612

Protein sequence:

>613_residues
MASRPLPLVTQDQTMHPYRTHSCAALRASDAGQTARLSGWVHSKRDHGGLLFIDLRDHFGMTQIVIPAGSPVLEAVERVR
VESVLTITGEVVLRDAATKNPNLPTGEIELRAQQVDIQSSADVLPFQVAGHEHYPEDLRLTYRYIDLRRDKVHRNMMLRA
QVIASLRRRMTDQGFVEFQTPILTASSPEGARDFLVPARMHPGKFYALPQAPQQFKQLAMVAGFDRYFQIAPCFRDEAAR
ADRSPGEFYQLDFEMAFATQEDVFATLEPVMEGVFREFGGGRTVSAAPFERIPYATAMKLYGSDKPDLRNPLLLSDVTES
FANSGFGLFARIVADGGEVRAIPAPGAGDRPRAFYDKLNAWAREAGAGGLGYIIFDEEGGKGPIAKNLEPARVAAIREAT
GLKAGDAVFFAAGKGDEVVKFSGLVRTRIATELDLIEQDSFRFCWITDFPMYERNEETGQIDFSHNPFSMPQGGLEALNG
QDPLTITAYQYDIVCNGVELSSGAIRNHRPDVMIRAFELAGYPASEVEARFGGMLNAFRYGAPPHGGSAPGVDRIVMLLA
DEPNIREVILFPLNQQGEDLMMGAPAPVPAERLKELSLALNLPRPKPGVKAGE

Sequences:

>Translated_613_residues
MASRPLPLVTQDQTMHPYRTHSCAALRASDAGQTARLSGWVHSKRDHGGLLFIDLRDHFGMTQIVIPAGSPVLEAVERVR
VESVLTITGEVVLRDAATKNPNLPTGEIELRAQQVDIQSSADVLPFQVAGHEHYPEDLRLTYRYIDLRRDKVHRNMMLRA
QVIASLRRRMTDQGFVEFQTPILTASSPEGARDFLVPARMHPGKFYALPQAPQQFKQLAMVAGFDRYFQIAPCFRDEAAR
ADRSPGEFYQLDFEMAFATQEDVFATLEPVMEGVFREFGGGRTVSAAPFERIPYATAMKLYGSDKPDLRNPLLLSDVTES
FANSGFGLFARIVADGGEVRAIPAPGAGDRPRAFYDKLNAWAREAGAGGLGYIIFDEEGGKGPIAKNLEPARVAAIREAT
GLKAGDAVFFAAGKGDEVVKFSGLVRTRIATELDLIEQDSFRFCWITDFPMYERNEETGQIDFSHNPFSMPQGGLEALNG
QDPLTITAYQYDIVCNGVELSSGAIRNHRPDVMIRAFELAGYPASEVEARFGGMLNAFRYGAPPHGGSAPGVDRIVMLLA
DEPNIREVILFPLNQQGEDLMMGAPAPVPAERLKELSLALNLPRPKPGVKAGE
>Mature_612_residues
ASRPLPLVTQDQTMHPYRTHSCAALRASDAGQTARLSGWVHSKRDHGGLLFIDLRDHFGMTQIVIPAGSPVLEAVERVRV
ESVLTITGEVVLRDAATKNPNLPTGEIELRAQQVDIQSSADVLPFQVAGHEHYPEDLRLTYRYIDLRRDKVHRNMMLRAQ
VIASLRRRMTDQGFVEFQTPILTASSPEGARDFLVPARMHPGKFYALPQAPQQFKQLAMVAGFDRYFQIAPCFRDEAARA
DRSPGEFYQLDFEMAFATQEDVFATLEPVMEGVFREFGGGRTVSAAPFERIPYATAMKLYGSDKPDLRNPLLLSDVTESF
ANSGFGLFARIVADGGEVRAIPAPGAGDRPRAFYDKLNAWAREAGAGGLGYIIFDEEGGKGPIAKNLEPARVAAIREATG
LKAGDAVFFAAGKGDEVVKFSGLVRTRIATELDLIEQDSFRFCWITDFPMYERNEETGQIDFSHNPFSMPQGGLEALNGQ
DPLTITAYQYDIVCNGVELSSGAIRNHRPDVMIRAFELAGYPASEVEARFGGMLNAFRYGAPPHGGSAPGVDRIVMLLAD
EPNIREVILFPLNQQGEDLMMGAPAPVPAERLKELSLALNLPRPKPGVKAGE

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=599, Percent_Identity=34.7245409015025, Blast_Score=355, Evalue=8e-98,
Organism=Homo sapiens, GI45439306, Length=325, Percent_Identity=24.9230769230769, Blast_Score=93, Evalue=7e-19,
Organism=Homo sapiens, GI226491502, Length=261, Percent_Identity=24.5210727969349, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI5031815, Length=263, Percent_Identity=25.4752851711027, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI194272210, Length=263, Percent_Identity=25.4752851711027, Blast_Score=77, Evalue=4e-14,
Organism=Escherichia coli, GI1788173, Length=589, Percent_Identity=40.0679117147708, Blast_Score=417, Evalue=1e-118,
Organism=Escherichia coli, GI1789256, Length=289, Percent_Identity=26.2975778546713, Blast_Score=76, Evalue=8e-15,
Organism=Escherichia coli, GI1790571, Length=219, Percent_Identity=27.8538812785388, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI32566633, Length=605, Percent_Identity=34.3801652892562, Blast_Score=305, Evalue=4e-83,
Organism=Caenorhabditis elegans, GI17551876, Length=278, Percent_Identity=29.136690647482, Blast_Score=92, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71994340, Length=269, Percent_Identity=25.6505576208178, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17535927, Length=270, Percent_Identity=26.2962962962963, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17535925, Length=270, Percent_Identity=26.2962962962963, Blast_Score=80, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6325153, Length=348, Percent_Identity=34.4827586206897, Blast_Score=164, Evalue=5e-41,
Organism=Saccharomyces cerevisiae, GI6323011, Length=271, Percent_Identity=28.0442804428044, Blast_Score=84, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6321807, Length=299, Percent_Identity=23.7458193979933, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24584738, Length=602, Percent_Identity=35.3820598006645, Blast_Score=319, Evalue=3e-87,
Organism=Drosophila melanogaster, GI17136276, Length=257, Percent_Identity=26.4591439688716, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24640851, Length=277, Percent_Identity=24.5487364620939, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24640849, Length=277, Percent_Identity=24.5487364620939, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 67413; Mature: 67282

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASRPLPLVTQDQTMHPYRTHSCAALRASDAGQTARLSGWVHSKRDHGGLLFIDLRDHFG
CCCCCCCEEECCCCCCCCCCCCEEEEECCCCCCEEEEECCEECCCCCCCEEEEEECCCCC
MTQIVIPAGSPVLEAVERVRVESVLTITGEVVLRDAATKNPNLPTGEIELRAQQVDIQSS
CEEEEEECCCHHHHHHHHHHHHHEEEEECEEEEEECCCCCCCCCCCEEEEEEEEECCCCC
ADVLPFQVAGHEHYPEDLRLTYRYIDLRRDKVHRNMMLRAQVIASLRRRMTDQGFVEFQT
CCCCEEEECCCCCCCHHHEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEC
PILTASSPEGARDFLVPARMHPGKFYALPQAPQQFKQLAMVAGFDRYFQIAPCFRDEAAR
CEEECCCCCCCHHCEEECCCCCCEEEECCCCHHHHHHHHHHHCCCHHEEECCCHHHHHHH
ADRSPGEFYQLDFEMAFATQEDVFATLEPVMEGVFREFGGGRTVSAAPFERIPYATAMKL
CCCCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCEECCCCHHHCCHHHHHHH
YGSDKPDLRNPLLLSDVTESFANSGFGLFARIVADGGEVRAIPAPGAGDRPRAFYDKLNA
HCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCEEEECCCCCCCCCHHHHHHHHH
WAREAGAGGLGYIIFDEEGGKGPIAKNLEPARVAAIREATGLKAGDAVFFAAGKGDEVVK
HHHHCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCEEE
FSGLVRTRIATELDLIEQDSFRFCWITDFPMYERNEETGQIDFSHNPFSMPQGGLEALNG
EHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCEEEECCCCCCCCCCCHHHCCC
QDPLTITAYQYDIVCNGVELSSGAIRNHRPDVMIRAFELAGYPASEVEARFGGMLNAFRY
CCCEEEEEEEEEEEECCEEECCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHC
GAPPHGGSAPGVDRIVMLLADEPNIREVILFPLNQQGEDLMMGAPAPVPAERLKELSLAL
CCCCCCCCCCCCCCEEEEEECCCCCEEEEEEECCCCCCCEEECCCCCCCHHHHHHHHHEE
NLPRPKPGVKAGE
ECCCCCCCCCCCC
>Mature Secondary Structure 
ASRPLPLVTQDQTMHPYRTHSCAALRASDAGQTARLSGWVHSKRDHGGLLFIDLRDHFG
CCCCCCEEECCCCCCCCCCCCEEEEECCCCCCEEEEECCEECCCCCCCEEEEEECCCCC
MTQIVIPAGSPVLEAVERVRVESVLTITGEVVLRDAATKNPNLPTGEIELRAQQVDIQSS
CEEEEEECCCHHHHHHHHHHHHHEEEEECEEEEEECCCCCCCCCCCEEEEEEEEECCCCC
ADVLPFQVAGHEHYPEDLRLTYRYIDLRRDKVHRNMMLRAQVIASLRRRMTDQGFVEFQT
CCCCEEEECCCCCCCHHHEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEC
PILTASSPEGARDFLVPARMHPGKFYALPQAPQQFKQLAMVAGFDRYFQIAPCFRDEAAR
CEEECCCCCCCHHCEEECCCCCCEEEECCCCHHHHHHHHHHHCCCHHEEECCCHHHHHHH
ADRSPGEFYQLDFEMAFATQEDVFATLEPVMEGVFREFGGGRTVSAAPFERIPYATAMKL
CCCCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCEECCCCHHHCCHHHHHHH
YGSDKPDLRNPLLLSDVTESFANSGFGLFARIVADGGEVRAIPAPGAGDRPRAFYDKLNA
HCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCEEEECCCCCCCCCHHHHHHHHH
WAREAGAGGLGYIIFDEEGGKGPIAKNLEPARVAAIREATGLKAGDAVFFAAGKGDEVVK
HHHHCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCEEE
FSGLVRTRIATELDLIEQDSFRFCWITDFPMYERNEETGQIDFSHNPFSMPQGGLEALNG
EHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCEEEECCCCCCCCCCCHHHCCC
QDPLTITAYQYDIVCNGVELSSGAIRNHRPDVMIRAFELAGYPASEVEARFGGMLNAFRY
CCCEEEEEEEEEEEECCEEECCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHC
GAPPHGGSAPGVDRIVMLLADEPNIREVILFPLNQQGEDLMMGAPAPVPAERLKELSLAL
CCCCCCCCCCCCCCEEEEEECCCCCEEEEEEECCCCCCCEEECCCCCCCHHHHHHHHHEE
NLPRPKPGVKAGE
ECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA