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The map label for this gene is aroKB [H]

Identifier: 162147968

GI number: 162147968

Start: 2233680

End: 2235455

Strand: Direct

Name: aroKB [H]

Synonym: GDI_2184

Alternate gene names: 162147968

Gene position: 2233680-2235455 (Clockwise)

Preceding gene: 162147964

Following gene: 162147969

Centisome position: 56.63

GC content: 70.1

Gene sequence:

>1776_bases
ATGTCACGTCCGATCGAACCGCCAGCCCCCGAGAGTACCGACCTGTCCCCCGCCGGGGGGCGCGACGCCCCGTTCCTGGC
GTTCGACCTCGATGCCCTGGTTGCCGCCGACAGGCCCGACAGGCCGGGCCCGCGCGCCCCGGGACCGCACGAGCGCAGCG
TCGTGCTGGTCGGGCTGATGGGGGCGGGCAAGACGACGATCGGCCGGCGGATCGCGGCACGGCTGGGCATGCCCTTCGTC
GATGCCGACGTGGAAATCGAGCGCGCGGCCGGATGTTCCATCGCCGACCTGTTCCGCCGGTATGGCGAGGCCGAATTCCG
CAAGGGCGAGCACCGCGTCATCCGCCGCATCCTGAGCGGCCACCCGCTGGTGCTGGCGACGGGCGGCGGCGCGTTCATGG
ACCCGGTGACCCGCGCGGTCATCCGCGACCGCGCGACCTCGGTCTGGCTGCGGTGCCCGCTGCCGGTGCTGGTGCGCCGC
GTCCAGGGGCGGACCCACCGGCCCCTGCTGAACGAGGGCAACCCGCGCGACATCCTGGCGGCCCTGATGGAAATCCGGCA
TCCGGTCTATGCCGAAGCGAACATCACCGTGGATTGTGGAGAAGAAAGCGTGGACCAGAGTGCCGCCACCGTCATCAGCG
CCCTGACCCTGGCGAAGCCGCCGCGACTCGTCCCCGTGATCCTGGAACGCTGGCGCTACGACGTGACCATCGGCGAGGAC
CTGCTGCGCCATGCCGGAATCCTGCTGGCCCCGGTCCTGCCGCAAAAGCGTGTGGTGGTCGTGACCGATTCGACCGTCGC
GACGCTGCACCTGCCGCGCCTGCTGGCCGGGCTGGCCGAGGGCGCGATCCGGGCGGAAACGATCGTCGTCCCGCCCGGGG
AAGGGTCGAAGACAATGGCCGAATACGAGCGCGTGACCAACGCGCTGCTGGACATGGGGGTCGAGCGCGGCACCACGGTG
ATCGCCCTGGGCGGCGGCGTGGTGGGCGACCTGGCGGGATTTGCCGCCGCCACCACCCTGCGCGGCCTGCCCTTCGTGCA
GATCCCGACGACGCTGCTGTCGCAGGTCGATTCGTCGGTGGGCGGCAAGACGGGGATCAACACCCCGTTCGGCAAGAACC
TGCTGGGCGCCTTCCATCAGCCGCTGGCGGTGCTGGTGGATACCACGACGCTGGCCAGCCTGCCGGCGCGCGAGGTCCGC
GCCGGCTATGCCGAGATCGTCAAATCCGGCCTGATCGGCGATGCCGCCCTGTTCGAATGGTGCGAAGCCAACGGCCAGGC
CGTACTGGACGGTGACGCCGATATCCGGGCCGAGGCCGTCCGCCAGGCCTGCGCGTTCAAGGCCCATGTCGTCGGCGACG
ACGAGCGGGAAGAAAAGAAATCGGACGGCCGCGCACTACTCAACCTGGGCCATACGTTCGGCCACGCGCTGGAGGCCGAA
CTGGGCTATGACGGCCGCCTGCTGCATGGCGAGGCCGTGTCGATCGGCCTGCGCCTGGCGTTCCTGGCGTCGGTCCGGAT
GGGGTTCTGCGACCGCACCGACCTGAACCGCGTCACCGCCCATCTGGAGCGGCTGGGCATGCCGGCGCGGATCAGCGACG
TTGGCGAAACGTTCTCGGCCGACCGGTTGATCGCGCATATGCAGCGGGACAAGAAAATGCGCGACGGACGCCTGTCCTTC
GTCCTGGTGCGCGGGATCGGGCAGGCCTTCACCTGCCGCGACGTCCCGGACGCGGTGGTCCGCGATATTCTTTTGGCGGA
AGGATGCGCGGCCTGA

Upstream 100 bases:

>100_bases
CCAACCCCGACGCGATGACGCCACGCGGCGGCGCGAGCGGGCAGGACCGTTCGCGAACCGTCATGGCTTGGTGCCTCTGC
TGTATGTTAGGTTTGCGATC

Downstream 100 bases:

>100_bases
ACAGGCCGCCTTCCTGATTGCGGCTTCTCTGTGGCGGCGGGACCACATATGTCGCGATTACCGCACACCGGCGTGGCCTG
CCCGCGGTCGACCACCGCCG

Product: bifunctional protein: shikimate kinase and 3-dehydroquinate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 591; Mature: 590

Protein sequence:

>591_residues
MSRPIEPPAPESTDLSPAGGRDAPFLAFDLDALVAADRPDRPGPRAPGPHERSVVLVGLMGAGKTTIGRRIAARLGMPFV
DADVEIERAAGCSIADLFRRYGEAEFRKGEHRVIRRILSGHPLVLATGGGAFMDPVTRAVIRDRATSVWLRCPLPVLVRR
VQGRTHRPLLNEGNPRDILAALMEIRHPVYAEANITVDCGEESVDQSAATVISALTLAKPPRLVPVILERWRYDVTIGED
LLRHAGILLAPVLPQKRVVVVTDSTVATLHLPRLLAGLAEGAIRAETIVVPPGEGSKTMAEYERVTNALLDMGVERGTTV
IALGGGVVGDLAGFAAATTLRGLPFVQIPTTLLSQVDSSVGGKTGINTPFGKNLLGAFHQPLAVLVDTTTLASLPAREVR
AGYAEIVKSGLIGDAALFEWCEANGQAVLDGDADIRAEAVRQACAFKAHVVGDDEREEKKSDGRALLNLGHTFGHALEAE
LGYDGRLLHGEAVSIGLRLAFLASVRMGFCDRTDLNRVTAHLERLGMPARISDVGETFSADRLIAHMQRDKKMRDGRLSF
VLVRGIGQAFTCRDVPDAVVRDILLAEGCAA

Sequences:

>Translated_591_residues
MSRPIEPPAPESTDLSPAGGRDAPFLAFDLDALVAADRPDRPGPRAPGPHERSVVLVGLMGAGKTTIGRRIAARLGMPFV
DADVEIERAAGCSIADLFRRYGEAEFRKGEHRVIRRILSGHPLVLATGGGAFMDPVTRAVIRDRATSVWLRCPLPVLVRR
VQGRTHRPLLNEGNPRDILAALMEIRHPVYAEANITVDCGEESVDQSAATVISALTLAKPPRLVPVILERWRYDVTIGED
LLRHAGILLAPVLPQKRVVVVTDSTVATLHLPRLLAGLAEGAIRAETIVVPPGEGSKTMAEYERVTNALLDMGVERGTTV
IALGGGVVGDLAGFAAATTLRGLPFVQIPTTLLSQVDSSVGGKTGINTPFGKNLLGAFHQPLAVLVDTTTLASLPAREVR
AGYAEIVKSGLIGDAALFEWCEANGQAVLDGDADIRAEAVRQACAFKAHVVGDDEREEKKSDGRALLNLGHTFGHALEAE
LGYDGRLLHGEAVSIGLRLAFLASVRMGFCDRTDLNRVTAHLERLGMPARISDVGETFSADRLIAHMQRDKKMRDGRLSF
VLVRGIGQAFTCRDVPDAVVRDILLAEGCAA
>Mature_590_residues
SRPIEPPAPESTDLSPAGGRDAPFLAFDLDALVAADRPDRPGPRAPGPHERSVVLVGLMGAGKTTIGRRIAARLGMPFVD
ADVEIERAAGCSIADLFRRYGEAEFRKGEHRVIRRILSGHPLVLATGGGAFMDPVTRAVIRDRATSVWLRCPLPVLVRRV
QGRTHRPLLNEGNPRDILAALMEIRHPVYAEANITVDCGEESVDQSAATVISALTLAKPPRLVPVILERWRYDVTIGEDL
LRHAGILLAPVLPQKRVVVVTDSTVATLHLPRLLAGLAEGAIRAETIVVPPGEGSKTMAEYERVTNALLDMGVERGTTVI
ALGGGVVGDLAGFAAATTLRGLPFVQIPTTLLSQVDSSVGGKTGINTPFGKNLLGAFHQPLAVLVDTTTLASLPAREVRA
GYAEIVKSGLIGDAALFEWCEANGQAVLDGDADIRAEAVRQACAFKAHVVGDDEREEKKSDGRALLNLGHTFGHALEAEL
GYDGRLLHGEAVSIGLRLAFLASVRMGFCDRTDLNRVTAHLERLGMPARISDVGETFSADRLIAHMQRDKKMRDGRLSFV
LVRGIGQAFTCRDVPDAVVRDILLAEGCAA

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; second step. [C]

COG id: COG0337

COG function: function code E; 3-dehydroquinate synthetase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dehydroquinate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1789791, Length=348, Percent_Identity=44.8275862068966, Blast_Score=266, Evalue=4e-72,
Organism=Escherichia coli, GI87082255, Length=168, Percent_Identity=38.6904761904762, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI1786587, Length=153, Percent_Identity=30.0653594771242, Blast_Score=64, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6320332, Length=394, Percent_Identity=34.2639593908629, Blast_Score=186, Evalue=7e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016303
- InterPro:   IPR002658
- InterPro:   IPR016037 [H]

Pfam domain/function: PF01761 DHQ_synthase [H]

EC number: =4.2.3.4 [H]

Molecular weight: Translated: 63250; Mature: 63118

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS01128 SHIKIMATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRPIEPPAPESTDLSPAGGRDAPFLAFDLDALVAADRPDRPGPRAPGPHERSVVLVGLM
CCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEC
GAGKTTIGRRIAARLGMPFVDADVEIERAAGCSIADLFRRYGEAEFRKGEHRVIRRILSG
CCCCHHHHHHHHHHHCCCEECCCCEEHHHCCCCHHHHHHHHCCHHHCCCHHHHHHHHHCC
HPLVLATGGGAFMDPVTRAVIRDRATSVWLRCPLPVLVRRVQGRTHRPLLNEGNPRDILA
CCEEEEECCCHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHCCCCCCCCCCCCCHHHHHH
ALMEIRHPVYAEANITVDCGEESVDQSAATVISALTLAKPPRLVPVILERWRYDVTIGED
HHHHHCCCCEECCEEEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHH
LLRHAGILLAPVLPQKRVVVVTDSTVATLHLPRLLAGLAEGAIRAETIVVPPGEGSKTMA
HHHHCCEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHH
EYERVTNALLDMGVERGTTVIALGGGVVGDLAGFAAATTLRGLPFVQIPTTLLSQVDSSV
HHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHC
GGKTGINTPFGKNLLGAFHQPLAVLVDTTTLASLPAREVRAGYAEIVKSGLIGDAALFEW
CCCCCCCCCCCHHHHHHHHCCCEEEEECHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHH
CEANGQAVLDGDADIRAEAVRQACAFKAHVVGDDEREEKKSDGRALLNLGHTFGHALEAE
HHCCCCEEECCCCCHHHHHHHHHHHHEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHHHC
LGYDGRLLHGEAVSIGLRLAFLASVRMGFCDRTDLNRVTAHLERLGMPARISDVGETFSA
CCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHH
DRLIAHMQRDKKMRDGRLSFVLVRGIGQAFTCRDVPDAVVRDILLAEGCAA
HHHHHHHHHHHHHCCCCEEEEEEECCCCCEECCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SRPIEPPAPESTDLSPAGGRDAPFLAFDLDALVAADRPDRPGPRAPGPHERSVVLVGLM
CCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEC
GAGKTTIGRRIAARLGMPFVDADVEIERAAGCSIADLFRRYGEAEFRKGEHRVIRRILSG
CCCCHHHHHHHHHHHCCCEECCCCEEHHHCCCCHHHHHHHHCCHHHCCCHHHHHHHHHCC
HPLVLATGGGAFMDPVTRAVIRDRATSVWLRCPLPVLVRRVQGRTHRPLLNEGNPRDILA
CCEEEEECCCHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHCCCCCCCCCCCCCHHHHHH
ALMEIRHPVYAEANITVDCGEESVDQSAATVISALTLAKPPRLVPVILERWRYDVTIGED
HHHHHCCCCEECCEEEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHH
LLRHAGILLAPVLPQKRVVVVTDSTVATLHLPRLLAGLAEGAIRAETIVVPPGEGSKTMA
HHHHCCEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHH
EYERVTNALLDMGVERGTTVIALGGGVVGDLAGFAAATTLRGLPFVQIPTTLLSQVDSSV
HHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHC
GGKTGINTPFGKNLLGAFHQPLAVLVDTTTLASLPAREVRAGYAEIVKSGLIGDAALFEW
CCCCCCCCCCCHHHHHHHHCCCEEEEECHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHH
CEANGQAVLDGDADIRAEAVRQACAFKAHVVGDDEREEKKSDGRALLNLGHTFGHALEAE
HHCCCCEEECCCCCHHHHHHHHHHHHEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHHHC
LGYDGRLLHGEAVSIGLRLAFLASVRMGFCDRTDLNRVTAHLERLGMPARISDVGETFSA
CCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHH
DRLIAHMQRDKKMRDGRLSFVLVRGIGQAFTCRDVPDAVVRDILLAEGCAA
HHHHHHHHHHHHHCCCCEEEEEEECCCCCEECCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA