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The map label for this gene is gloA [H]

Identifier: 162147923

GI number: 162147923

Start: 2191925

End: 2192314

Strand: Reverse

Name: gloA [H]

Synonym: GDI_2139

Alternate gene names: 162147923

Gene position: 2192314-2191925 (Counterclockwise)

Preceding gene: 162147924

Following gene: 162147921

Centisome position: 55.58

GC content: 63.08

Gene sequence:

>390_bases
ATGGGATCGTATCTGCACACGATGGTTCGCGTCCGCAATCTCGACGCCAGCATTGCCTTCTATAAATTGCTGGGCATGCA
CGAATTGCGCCGGCGCGAGGTGCCCGAAGGCAAATACACCCTTGTCTTCATCGGCTATGCCGACAACGCGGCCGGCCAGG
CGGAAATCGAACTGACCTATAACTGGGGCCAGGATGACGGGTACGAGGTCGGGACCGGATTCGGTCATTTCGCCGTAGGC
GTGCCCGACGTGGCGGCGGTGGCCGAGGCCGTGCGCGCGGGCGGCGGCAAGGTGACGCGCGAGGCCGGGCCCGTGAAATT
CGGCACCACGATCATCGCCTTCGTGGAAGACCCGGACGGGTACAAGATCGAATTGATCCAGCGCCCGTAG

Upstream 100 bases:

>100_bases
CTTCCAGGCCGGTGCCACCCTGTTGACCGATCCGGACGGCTGCGTCGCGGCCGCGAATGAAGCCGGCCTGTTCCTGGCCG
GGATCGAATAGGAGAGAAAG

Downstream 100 bases:

>100_bases
GCGCCCGGTCATTTTTTGGGCTGTCCAAGCGCCTTCAGCCGGCTTACGGCCTGCTCGATGGACTGGATGATCTGTTCGGC
GGCGGCGCGGACCTGCGGGT

Product: lactoylglutathione lyase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 129; Mature: 128

Protein sequence:

>129_residues
MGSYLHTMVRVRNLDASIAFYKLLGMHELRRREVPEGKYTLVFIGYADNAAGQAEIELTYNWGQDDGYEVGTGFGHFAVG
VPDVAAVAEAVRAGGGKVTREAGPVKFGTTIIAFVEDPDGYKIELIQRP

Sequences:

>Translated_129_residues
MGSYLHTMVRVRNLDASIAFYKLLGMHELRRREVPEGKYTLVFIGYADNAAGQAEIELTYNWGQDDGYEVGTGFGHFAVG
VPDVAAVAEAVRAGGGKVTREAGPVKFGTTIIAFVEDPDGYKIELIQRP
>Mature_128_residues
GSYLHTMVRVRNLDASIAFYKLLGMHELRRREVPEGKYTLVFIGYADNAAGQAEIELTYNWGQDDGYEVGTGFGHFAVGV
PDVAAVAEAVRAGGGKVTREAGPVKFGTTIIAFVEDPDGYKIELIQRP

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=145, Percent_Identity=31.7241379310345, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI1787940, Length=124, Percent_Identity=61.2903225806452, Blast_Score=143, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6323639, Length=140, Percent_Identity=32.1428571428571, Blast_Score=76, Evalue=1e-15,
Organism=Drosophila melanogaster, GI20129743, Length=147, Percent_Identity=32.6530612244898, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 14067; Mature: 13936

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS00934 GLYOXALASE_I_1 ; PS00935 GLYOXALASE_I_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSYLHTMVRVRNLDASIAFYKLLGMHELRRREVPEGKYTLVFIGYADNAAGQAEIELTY
CCHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHCCCCCCEEEEEEEECCCCCCEEEEEEEE
NWGQDDGYEVGTGFGHFAVGVPDVAAVAEAVRAGGGKVTREAGPVKFGTTIIAFVEDPDG
ECCCCCCEEECCCCCEEEECCCCHHHHHHHHHCCCCEEECCCCCEEECCEEEEEEECCCC
YKIELIQRP
CEEEEEECC
>Mature Secondary Structure 
GSYLHTMVRVRNLDASIAFYKLLGMHELRRREVPEGKYTLVFIGYADNAAGQAEIELTY
CHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHCCCCCCEEEEEEEECCCCCCEEEEEEEE
NWGQDDGYEVGTGFGHFAVGVPDVAAVAEAVRAGGGKVTREAGPVKFGTTIIAFVEDPDG
ECCCCCCEEECCCCCEEEECCCCHHHHHHHHHCCCCEEECCCCCEEECCEEEEEEECCCC
YKIELIQRP
CEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 9719565 [H]