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The map label for this gene is rpoN [H]

Identifier: 162147903

GI number: 162147903

Start: 2173760

End: 2175136

Strand: Direct

Name: rpoN [H]

Synonym: GDI_2119

Alternate gene names: 162147903

Gene position: 2173760-2175136 (Clockwise)

Preceding gene: 162147902

Following gene: 162147904

Centisome position: 55.11

GC content: 68.12

Gene sequence:

>1377_bases
ATGACGATCGGCCCCAGGCTCGCGCTCCGGCAGGCCCAGACCCTCGTCATGACGCCGCAATTGCGGCAGGCGATCCACCT
TCTGCAATTGTCCAACCTCGACGCCTGTTCGTTCATCGAACAGGAACTGGAACGCAATCCGCTGCTGGAGCGCGCGAACC
TGGCGGGGCTCGAGGAACTCCGGCCCAAGGTCGTGGCCCACGGTGCGCGCCATGACCCTCGCCTGGAACGGGTCCCCGAC
CAGGACTGGGCCGCCACGGCGGGGACCCTGCCCGGCCCGGCCCCCTCGCTGCACGAACGGCTGGCGCAACAGGTGCGGCT
GTCGTGGCGCGATGCCGGCGACCGGCTGGTAGGGGCGTATCTGATCACCCTGCTGGACGCCGACGGGCGGCTCGTCCCCC
CGACGGCGGTCATCGCCCAGTCCCTGGGCACCACGTTCGACCATGTCGAGCGTGTGCGCCAGACCATGATGCAGTTCGAG
CCGACGGGCCTGTTCGCCCACGATCTGGGCGAATGCCTGGCCGCCCAGTTGCGCGAACGCGACCGGCTGGACCCGGCGAT
GGCCGTCCTGCTGCGGCACCTGGACCTGCTGGCCCGGCGCGACCTGCGCCGGCTGCAGGGTCTGTGCGGCGTCGATGCCG
AGGACCTGGCGGACATGATTGCCGAGGTCCGCACCCTGTCCCCCCGCCCGGCCCGGGATTTCGGCGATCCGGCGGCCTGT
TCCGTCATCCCGGACGTCCTGTTGCAGCCCGCGCCGGACGGGGACTGGATGCTGGAACTCAACCCCGAGACCATGCCGAG
CGTGGTGGTGAATTCCGCATTGAGCACCCGGGTGGCGCTGCGGGCCCGGCGCGAGGACCGGCCGTTCCTGAACGAGCGCC
TGGCCAGCGCCAACTGGCTGGTGCGCGCCCTGCAGCAGCGGGCCGACACGATCATCCGCGTCGCGACCGCGATCATGCGC
CGGCAGCGCGGCTTCCTGGATCACGGGATCGGCCACCTGCGCCCGCTGGTGCTGCGCGATATCGCCGAAACGGTCGGGCT
GCACGAAAGCACCGTCAGCCGGGTGACGGCGAACAAGTACATCGCCACCCCGCGCGGCCTGTTCGAGCTGAAATATTTCT
TCACCACCGCGATCCCGGGCCGCGCGGGCGGCGCCGACCACAGCGCCGAGGCCATCCGCCATCGGATCAAGACCTTGATT
TCACAGGAATCTGATAGAAAAATCCTGTCCGACGATGCAATCGTGACCCGGTTACGCAAGGAAGGAATAGACATTTCTCG
CCGAACCGTGGCCAAATACCGGGATGCGCTGAGGATACCCAACTCGACGCAGAGAAAACGGGACAAGGCCCTCATCGGCC
TGAAACCGGAAGCTTGA

Upstream 100 bases:

>100_bases
GGCCAGGTGCTGATGGAAGGCCGCCCCGAGGAAATCGTCGCCAACGAGGACGTCCGACGGGTGTACCTTGGGGAGAATTT
CTCCCTCTGACCGTCTGTCC

Downstream 100 bases:

>100_bases
GGCCCGAATCCCGACGCCATGACCGGCAGTTTGGAGCGCCGGCATGCATCGTCCGGTTTCGGACGCTCCTCCTCCATGCA
AGGGGTGATGTCACATGCAG

Product: RNA polymerase factor sigma-54

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MTIGPRLALRQAQTLVMTPQLRQAIHLLQLSNLDACSFIEQELERNPLLERANLAGLEELRPKVVAHGARHDPRLERVPD
QDWAATAGTLPGPAPSLHERLAQQVRLSWRDAGDRLVGAYLITLLDADGRLVPPTAVIAQSLGTTFDHVERVRQTMMQFE
PTGLFAHDLGECLAAQLRERDRLDPAMAVLLRHLDLLARRDLRRLQGLCGVDAEDLADMIAEVRTLSPRPARDFGDPAAC
SVIPDVLLQPAPDGDWMLELNPETMPSVVVNSALSTRVALRARREDRPFLNERLASANWLVRALQQRADTIIRVATAIMR
RQRGFLDHGIGHLRPLVLRDIAETVGLHESTVSRVTANKYIATPRGLFELKYFFTTAIPGRAGGADHSAEAIRHRIKTLI
SQESDRKILSDDAIVTRLRKEGIDISRRTVAKYRDALRIPNSTQRKRDKALIGLKPEA

Sequences:

>Translated_458_residues
MTIGPRLALRQAQTLVMTPQLRQAIHLLQLSNLDACSFIEQELERNPLLERANLAGLEELRPKVVAHGARHDPRLERVPD
QDWAATAGTLPGPAPSLHERLAQQVRLSWRDAGDRLVGAYLITLLDADGRLVPPTAVIAQSLGTTFDHVERVRQTMMQFE
PTGLFAHDLGECLAAQLRERDRLDPAMAVLLRHLDLLARRDLRRLQGLCGVDAEDLADMIAEVRTLSPRPARDFGDPAAC
SVIPDVLLQPAPDGDWMLELNPETMPSVVVNSALSTRVALRARREDRPFLNERLASANWLVRALQQRADTIIRVATAIMR
RQRGFLDHGIGHLRPLVLRDIAETVGLHESTVSRVTANKYIATPRGLFELKYFFTTAIPGRAGGADHSAEAIRHRIKTLI
SQESDRKILSDDAIVTRLRKEGIDISRRTVAKYRDALRIPNSTQRKRDKALIGLKPEA
>Mature_457_residues
TIGPRLALRQAQTLVMTPQLRQAIHLLQLSNLDACSFIEQELERNPLLERANLAGLEELRPKVVAHGARHDPRLERVPDQ
DWAATAGTLPGPAPSLHERLAQQVRLSWRDAGDRLVGAYLITLLDADGRLVPPTAVIAQSLGTTFDHVERVRQTMMQFEP
TGLFAHDLGECLAAQLRERDRLDPAMAVLLRHLDLLARRDLRRLQGLCGVDAEDLADMIAEVRTLSPRPARDFGDPAACS
VIPDVLLQPAPDGDWMLELNPETMPSVVVNSALSTRVALRARREDRPFLNERLASANWLVRALQQRADTIIRVATAIMRR
QRGFLDHGIGHLRPLVLRDIAETVGLHESTVSRVTANKYIATPRGLFELKYFFTTAIPGRAGGADHSAEAIRHRIKTLIS
QESDRKILSDDAIVTRLRKEGIDISRRTVAKYRDALRIPNSTQRKRDKALIGLKPEA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of the nitrogen fixation genes [H]

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=475, Percent_Identity=36.2105263157895, Blast_Score=276, Evalue=2e-75,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 51122; Mature: 50990

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIGPRLALRQAQTLVMTPQLRQAIHLLQLSNLDACSFIEQELERNPLLERANLAGLEEL
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHCCCCHHHH
RPKVVAHGARHDPRLERVPDQDWAATAGTLPGPAPSLHERLAQQVRLSWRDAGDRLVGAY
HHHHHHCCCCCCCCCCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LITLLDADGRLVPPTAVIAQSLGTTFDHVERVRQTMMQFEPTGLFAHDLGECLAAQLRER
HHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
DRLDPAMAVLLRHLDLLARRDLRRLQGLCGVDAEDLADMIAEVRTLSPRPARDFGDPAAC
HCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHH
SVIPDVLLQPAPDGDWMLELNPETMPSVVVNSALSTRVALRARREDRPFLNERLASANWL
HHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
VRALQQRADTIIRVATAIMRRQRGFLDHGIGHLRPLVLRDIAETVGLHESTVSRVTANKY
HHHHHHHHHHHHHHHHHHHHHHHCHHHHCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
IATPRGLFELKYFFTTAIPGRAGGADHSAEAIRHRIKTLISQESDRKILSDDAIVTRLRK
CCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
EGIDISRRTVAKYRDALRIPNSTQRKRDKALIGLKPEA
HCCCHHHHHHHHHHHHHCCCCHHHHHHHCCEECCCCCC
>Mature Secondary Structure 
TIGPRLALRQAQTLVMTPQLRQAIHLLQLSNLDACSFIEQELERNPLLERANLAGLEEL
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHCCCCHHHH
RPKVVAHGARHDPRLERVPDQDWAATAGTLPGPAPSLHERLAQQVRLSWRDAGDRLVGAY
HHHHHHCCCCCCCCCCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LITLLDADGRLVPPTAVIAQSLGTTFDHVERVRQTMMQFEPTGLFAHDLGECLAAQLRER
HHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
DRLDPAMAVLLRHLDLLARRDLRRLQGLCGVDAEDLADMIAEVRTLSPRPARDFGDPAAC
HCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHH
SVIPDVLLQPAPDGDWMLELNPETMPSVVVNSALSTRVALRARREDRPFLNERLASANWL
HHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
VRALQQRADTIIRVATAIMRRQRGFLDHGIGHLRPLVLRDIAETVGLHESTVSRVTANKY
HHHHHHHHHHHHHHHHHHHHHHHCHHHHCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
IATPRGLFELKYFFTTAIPGRAGGADHSAEAIRHRIKTLISQESDRKILSDDAIVTRLRK
CCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
EGIDISRRTVAKYRDALRIPNSTQRKRDKALIGLKPEA
HCCCHHHHHHHHHHHHHCCCCHHHHHHHCCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1991712; 12597275 [H]