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The map label for this gene is npdA [H]

Identifier: 162147826

GI number: 162147826

Start: 2086714

End: 2087544

Strand: Direct

Name: npdA [H]

Synonym: GDI_2042

Alternate gene names: 162147826

Gene position: 2086714-2087544 (Clockwise)

Preceding gene: 162147825

Following gene: 162147827

Centisome position: 52.91

GC content: 67.51

Gene sequence:

>831_bases
TTGCCGCCGCGGGGCCCTACCCCGCGGCGGTTTTCTTATGGGCCCGATCTGGAGCACAATAGGGCCATCCCGCCGAGGAG
GAGATCGCCCATGGAACGCCCGCTGGAACGCCCGCTGGAACGCCCCTGGGAACGTATCGTCGTCCTGACCGGCGCCGGCA
TCAGCCGGGAATCGGGCCTGGAAACATTCCGTGACGGCGACGGACTCTGGAGTCGCTACGCCATCGAGGATGTGTGCACG
CCCCACGCCTTTCACCGCGACCCGTTGCTGGTGGACCGCTTCTATAACGACAGGCGTGCCGAATTGTGGCGCGTGCGGCC
CAACGACGCGCATCTGGCCCTGGCCCGGCTGGAGCACGCGGCCCGGGCGGGGGAATGGGACGGCGAGATCCTGATCGTCA
CGCAGAATATCGACGATCTGCATGAACGGGCCGGCAGCCGGAACGTGGTGCATATGCACGGCGAATTGCTGCGCGTGCGC
TGTACCGCCTGCGGCGCGACGCCTGACTGGCAGGAAAACTGCCTGCCGCAGACCCCATGCCCGATATGCCATCGCCCGGC
GCTGCGTCCCGATGTCGTATGGTTCGGCGAAATTCCGCTGCACATGGAGCGGATTCAGGCCGCGCTGGACCGATGCGACC
GCTTCGTGTCCATCGGCACGTCCGGCACGGTCTATCCGGCCGCCGGTTTCGTGGCGCAGGTGCGCGACCATGCCGACACA
CTGGACATCAATCTGGAGGCCCCCGCCGTTCCGACGGCGTTCGAGCGCAGCATGGTGGGCGCCGCCACCCGGATGGTGCC
GCGCTGGGTCGACGAAGTCCTGGCCGGATGA

Upstream 100 bases:

>100_bases
AGCCCTACGGCGCCGGGTGGACCTTCGCCCCGCAGACCCATTCCGGAGTCGACCAGGCGCAGTAACGCCACGCCGGTCAT
CGGGTAGCACAAGACGCTTC

Downstream 100 bases:

>100_bases
CCGGGAACGAGGACGATTTGGACGCGCTGTTGTGTCAGGTGCGGGCCTGCCGGGCCTGCGCCGACAGCCTGCCCCTGGGG
CCGCGGCCGATCCTGCACGT

Product: NAD-dependent deacetylase

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog [H]

Number of amino acids: Translated: 276; Mature: 275

Protein sequence:

>276_residues
MPPRGPTPRRFSYGPDLEHNRAIPPRRRSPMERPLERPLERPWERIVVLTGAGISRESGLETFRDGDGLWSRYAIEDVCT
PHAFHRDPLLVDRFYNDRRAELWRVRPNDAHLALARLEHAARAGEWDGEILIVTQNIDDLHERAGSRNVVHMHGELLRVR
CTACGATPDWQENCLPQTPCPICHRPALRPDVVWFGEIPLHMERIQAALDRCDRFVSIGTSGTVYPAAGFVAQVRDHADT
LDINLEAPAVPTAFERSMVGAATRMVPRWVDEVLAG

Sequences:

>Translated_276_residues
MPPRGPTPRRFSYGPDLEHNRAIPPRRRSPMERPLERPLERPWERIVVLTGAGISRESGLETFRDGDGLWSRYAIEDVCT
PHAFHRDPLLVDRFYNDRRAELWRVRPNDAHLALARLEHAARAGEWDGEILIVTQNIDDLHERAGSRNVVHMHGELLRVR
CTACGATPDWQENCLPQTPCPICHRPALRPDVVWFGEIPLHMERIQAALDRCDRFVSIGTSGTVYPAAGFVAQVRDHADT
LDINLEAPAVPTAFERSMVGAATRMVPRWVDEVLAG
>Mature_275_residues
PPRGPTPRRFSYGPDLEHNRAIPPRRRSPMERPLERPLERPWERIVVLTGAGISRESGLETFRDGDGLWSRYAIEDVCTP
HAFHRDPLLVDRFYNDRRAELWRVRPNDAHLALARLEHAARAGEWDGEILIVTQNIDDLHERAGSRNVVHMHGELLRVRC
TACGATPDWQENCLPQTPCPICHRPALRPDVVWFGEIPLHMERIQAALDRCDRFVSIGTSGTVYPAAGFVAQVRDHADTL
DINLEAPAVPTAFERSMVGAATRMVPRWVDEVLAG

Specific function: Modulates the activities of several enzymes which are inactive in their acetylated form [H]

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain [H]

Homologues:

Organism=Homo sapiens, GI300795542, Length=198, Percent_Identity=39.3939393939394, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI13787215, Length=216, Percent_Identity=37.5, Blast_Score=146, Evalue=2e-35,
Organism=Homo sapiens, GI6912664, Length=216, Percent_Identity=37.5, Blast_Score=146, Evalue=2e-35,
Organism=Homo sapiens, GI6912660, Length=224, Percent_Identity=32.5892857142857, Blast_Score=76, Evalue=3e-14,
Organism=Escherichia coli, GI308199517, Length=242, Percent_Identity=52.0661157024793, Blast_Score=243, Evalue=1e-65,
Organism=Caenorhabditis elegans, GI71990482, Length=234, Percent_Identity=29.4871794871795, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17567771, Length=262, Percent_Identity=26.7175572519084, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71990487, Length=236, Percent_Identity=29.2372881355932, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI28571445, Length=132, Percent_Identity=39.3939393939394, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003000 [H]

Pfam domain/function: PF02146 SIR2 [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 31260; Mature: 31129

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS50305 SIRTUIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPRGPTPRRFSYGPDLEHNRAIPPRRRSPMERPLERPLERPWERIVVLTGAGISRESGL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCHHHEEEEEECCCCCCHHCC
ETFRDGDGLWSRYAIEDVCTPHAFHRDPLLVDRFYNDRRAELWRVRPNDAHLALARLEHA
HHHHCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCEEEEECCCCHHHHHHHHHHH
ARAGEWDGEILIVTQNIDDLHERAGSRNVVHMHGELLRVRCTACGATPDWQENCLPQTPC
HHCCCCCCEEEEEECCHHHHHHHCCCCCEEEECCCEEEEEEEECCCCCCHHHCCCCCCCC
PICHRPALRPDVVWFGEIPLHMERIQAALDRCDRFVSIGTSGTVYPAAGFVAQVRDHADT
CCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHCCCE
LDINLEAPAVPTAFERSMVGAATRMVPRWVDEVLAG
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PPRGPTPRRFSYGPDLEHNRAIPPRRRSPMERPLERPLERPWERIVVLTGAGISRESGL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCHHHEEEEEECCCCCCHHCC
ETFRDGDGLWSRYAIEDVCTPHAFHRDPLLVDRFYNDRRAELWRVRPNDAHLALARLEHA
HHHHCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCEEEEECCCCHHHHHHHHHHH
ARAGEWDGEILIVTQNIDDLHERAGSRNVVHMHGELLRVRCTACGATPDWQENCLPQTPC
HHCCCCCCEEEEEECCHHHHHHHCCCCCEEEECCCEEEEEEEECCCCCCHHHCCCCCCCC
PICHRPALRPDVVWFGEIPLHMERIQAALDRCDRFVSIGTSGTVYPAAGFVAQVRDHADT
CCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHCCCE
LDINLEAPAVPTAFERSMVGAATRMVPRWVDEVLAG
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]