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The map label for this gene is gatA [H]

Identifier: 162147781

GI number: 162147781

Start: 2032326

End: 2033717

Strand: Reverse

Name: gatA [H]

Synonym: GDI_1997

Alternate gene names: 162147781

Gene position: 2033717-2032326 (Counterclockwise)

Preceding gene: 162147782

Following gene: 162147780

Centisome position: 51.56

GC content: 73.06

Gene sequence:

>1392_bases
GTGCGAACCCGCTTTCGAATACCGCCCATGAGCGCGCCCGTTGCGGCCGGTCCGGCCGATGCGCTGGCCATCGCGGCCGA
AATCCGCCAGGGGCGGCGCAATGCCGTATCCTTCGTCACCGCCGCCATCGCGGCGATCGAGGTCCGTGACACGCGGGTCA
ACAGCGTAACCCGCATCTTCGGCCCCCGCGCGGTGGCCGAGGCCGAGAAGATCGACCGCCGCATCGCGGCCGGCGAAGAC
CCCGGCCCGCTGGCCGGCGTGCCCTTCGGCGTCAAGGACCTGTTCGATGTCGAGGGCGAGGTCACGACCGCAGGGTCCGT
CGTCCTGCGCGAGGCGCGTCCGGCGGTGCAGGACGCCACCGTGATCGCGCGGCTGCGCGCGGCGGGGGCCATCCCGCTGG
CCAGCCTGAACATGGACGAATTCGCCTACGGCTTCGCCACCGACAACGCGCATTACGGCATCACCCGCAACCCGCATGAT
CCGGCGCGGCTGGCCGGCGGGTCGTCGGGCGGGTCCGCCGCCGCCGTGGCGGCCGGCTTCCTGCCGTTCACCCTGGGGTC
GGACACCAACGGGTCGATCCGCGTTCCGGCGTCGCTGTGCGGCGTGTGGGGGCTGAAGCCCACCTTCGGCACCGTCCCGC
GAGAAGGGGCGTATCCCTTCGCCGCCAGCCTGGACGTGGTGGGACCGTTCGCGGGTACGCTGGCCGACCTGCGCGTGGTC
CATGAAGCGATGAATGGCGCCCCGATGCCGGCCCTGCCGGTCCTGTCCGACCTGCGGGTGGCGCGGTTGGGCGGCTGGTT
CGCCGAAAACGTGTCCGCCCCGCTGGCGGCGGCCATCGACGCCGTGGCGGGCCATCTGGGCCATCTTCCGGTGGTGGAAC
TGCCGGAGGTCGCGCGGGCGCGTGCGGCGTCGTTCCTGATCACGGCGGCGGAAGGCGGCATGCTGCACCTGCCGCGCCTG
CGCCGCCGCGCCCTGCAATACGACCCGGCGACGCGCGACCGGCTGATCGCCGGCGCCCTGCTGCCCGCCGCGACGGTCCT
GCAGGCCCATCGCTTCCGTGCGTGGTTCCGCGAACAGATCCACGCCGCGTTCCGCACGGCCGATGTGCTGATCGCCCCCG
CCACGGTCGGCGAGGCCCCGCTGATCGACCAGCCGACCATCGTCGTGGACGGGCAGGCCGTTTCCGCCCGCGCCAACCTG
GGCCTGTATACCCAGCCGCTCAGCCTGGCCGGGGTGCCGATCCTCAGCGTTCCGCTGCTGGGCACCGGGGGTTTGCCGCT
CGGCCTGCAATTGATCGCAGCCCCGGGGGGCGAGCCCGCGTTGTTTGCCGTGGCCGCGGCGCTGGAAGCCGCCGGCGTGG
CCGGGAAGGTTCCGCTTCCGAAGGGACGATAG

Upstream 100 bases:

>100_bases
GCCTGACCATCGCCGACGTCTGCATGCCGGGCGTGCTGGCCAACCGGGCCCTGCTGCGGCGCTATGCCGACCTGGTCCAT
GGCTTCGCGCTGCCGGACAC

Downstream 100 bases:

>100_bases
ACCATGCTGCATACGCCACGTTCGCAGCGCGGGATGGTGACGTCCCCGCATCATCTGGCCAGCCAGGCCGGACTGGACGT
GCTGCGCGACGGCGGCACGG

Product: glutamyl-tRNA(Gln) amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MRTRFRIPPMSAPVAAGPADALAIAAEIRQGRRNAVSFVTAAIAAIEVRDTRVNSVTRIFGPRAVAEAEKIDRRIAAGED
PGPLAGVPFGVKDLFDVEGEVTTAGSVVLREARPAVQDATVIARLRAAGAIPLASLNMDEFAYGFATDNAHYGITRNPHD
PARLAGGSSGGSAAAVAAGFLPFTLGSDTNGSIRVPASLCGVWGLKPTFGTVPREGAYPFAASLDVVGPFAGTLADLRVV
HEAMNGAPMPALPVLSDLRVARLGGWFAENVSAPLAAAIDAVAGHLGHLPVVELPEVARARAASFLITAAEGGMLHLPRL
RRRALQYDPATRDRLIAGALLPAATVLQAHRFRAWFREQIHAAFRTADVLIAPATVGEAPLIDQPTIVVDGQAVSARANL
GLYTQPLSLAGVPILSVPLLGTGGLPLGLQLIAAPGGEPALFAVAAALEAAGVAGKVPLPKGR

Sequences:

>Translated_463_residues
MRTRFRIPPMSAPVAAGPADALAIAAEIRQGRRNAVSFVTAAIAAIEVRDTRVNSVTRIFGPRAVAEAEKIDRRIAAGED
PGPLAGVPFGVKDLFDVEGEVTTAGSVVLREARPAVQDATVIARLRAAGAIPLASLNMDEFAYGFATDNAHYGITRNPHD
PARLAGGSSGGSAAAVAAGFLPFTLGSDTNGSIRVPASLCGVWGLKPTFGTVPREGAYPFAASLDVVGPFAGTLADLRVV
HEAMNGAPMPALPVLSDLRVARLGGWFAENVSAPLAAAIDAVAGHLGHLPVVELPEVARARAASFLITAAEGGMLHLPRL
RRRALQYDPATRDRLIAGALLPAATVLQAHRFRAWFREQIHAAFRTADVLIAPATVGEAPLIDQPTIVVDGQAVSARANL
GLYTQPLSLAGVPILSVPLLGTGGLPLGLQLIAAPGGEPALFAVAAALEAAGVAGKVPLPKGR
>Mature_463_residues
MRTRFRIPPMSAPVAAGPADALAIAAEIRQGRRNAVSFVTAAIAAIEVRDTRVNSVTRIFGPRAVAEAEKIDRRIAAGED
PGPLAGVPFGVKDLFDVEGEVTTAGSVVLREARPAVQDATVIARLRAAGAIPLASLNMDEFAYGFATDNAHYGITRNPHD
PARLAGGSSGGSAAAVAAGFLPFTLGSDTNGSIRVPASLCGVWGLKPTFGTVPREGAYPFAASLDVVGPFAGTLADLRVV
HEAMNGAPMPALPVLSDLRVARLGGWFAENVSAPLAAAIDAVAGHLGHLPVVELPEVARARAASFLITAAEGGMLHLPRL
RRRALQYDPATRDRLIAGALLPAATVLQAHRFRAWFREQIHAAFRTADVLIAPATVGEAPLIDQPTIVVDGQAVSARANL
GLYTQPLSLAGVPILSVPLLGTGGLPLGLQLIAAPGGEPALFAVAAALEAAGVAGKVPLPKGR

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=260, Percent_Identity=31.9230769230769, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI222831590, Length=497, Percent_Identity=26.9617706237425, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI166795287, Length=135, Percent_Identity=38.5185185185185, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17543272, Length=432, Percent_Identity=27.3148148148148, Blast_Score=138, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI17537465, Length=257, Percent_Identity=33.852140077821, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17556264, Length=285, Percent_Identity=30.1754385964912, Blast_Score=107, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI17538252, Length=194, Percent_Identity=30.9278350515464, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17556276, Length=190, Percent_Identity=35.7894736842105, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17556278, Length=190, Percent_Identity=35.7894736842105, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71990152, Length=134, Percent_Identity=36.5671641791045, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17538254, Length=178, Percent_Identity=34.2696629213483, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319685, Length=154, Percent_Identity=44.8051948051948, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6323950, Length=433, Percent_Identity=25.635103926097, Blast_Score=87, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6320448, Length=210, Percent_Identity=30.4761904761905, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24652985, Length=495, Percent_Identity=29.0909090909091, Blast_Score=138, Evalue=7e-33,
Organism=Drosophila melanogaster, GI19922090, Length=495, Percent_Identity=29.0909090909091, Blast_Score=138, Evalue=7e-33,
Organism=Drosophila melanogaster, GI24652981, Length=495, Percent_Identity=29.0909090909091, Blast_Score=138, Evalue=7e-33,
Organism=Drosophila melanogaster, GI24652983, Length=495, Percent_Identity=29.0909090909091, Blast_Score=138, Evalue=7e-33,
Organism=Drosophila melanogaster, GI24648113, Length=461, Percent_Identity=31.236442516269, Blast_Score=138, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24644968, Length=481, Percent_Identity=28.0665280665281, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI21356731, Length=490, Percent_Identity=27.3469387755102, Blast_Score=125, Evalue=8e-29,
Organism=Drosophila melanogaster, GI24648435, Length=487, Percent_Identity=25.0513347022587, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24648437, Length=487, Percent_Identity=25.0513347022587, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI45550774, Length=487, Percent_Identity=25.0513347022587, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI161078093, Length=506, Percent_Identity=27.2727272727273, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24648441, Length=409, Percent_Identity=24.6943765281174, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24648439, Length=409, Percent_Identity=24.6943765281174, Blast_Score=99, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 47689; Mature: 47689

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTRFRIPPMSAPVAAGPADALAIAAEIRQGRRNAVSFVTAAIAAIEVRDTRVNSVTRIF
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHEEEECCHHHHHHHHH
GPRAVAEAEKIDRRIAAGEDPGPLAGVPFGVKDLFDVEGEVTTAGSVVLREARPAVQDAT
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCCEECCCHHHHHHCCCCHHHHH
VIARLRAAGAIPLASLNMDEFAYGFATDNAHYGITRNPHDPARLAGGSSGGSAAAVAAGF
HHHHHHHCCCCEEEECCHHHHEEEEECCCCCCCCCCCCCCHHHHCCCCCCCCHHHHHHCC
LPFTLGSDTNGSIRVPASLCGVWGLKPTFGTVPREGAYPFAASLDVVGPFAGTLADLRVV
EEEEECCCCCCCEECCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
HEAMNGAPMPALPVLSDLRVARLGGWFAENVSAPLAAAIDAVAGHLGHLPVVELPEVARA
HHHHCCCCCCHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHH
RAASFLITAAEGGMLHLPRLRRRALQYDPATRDRLIAGALLPAATVLQAHRFRAWFREQI
HHHEEEEEECCCCEEECHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HAAFRTADVLIAPATVGEAPLIDQPTIVVDGQAVSARANLGLYTQPLSLAGVPILSVPLL
HHHHHHHCEEEECCCCCCCCCCCCCEEEECCCEEEEECCCCEEECCHHHCCCCEEEECEE
GTGGLPLGLQLIAAPGGEPALFAVAAALEAAGVAGKVPLPKGR
CCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MRTRFRIPPMSAPVAAGPADALAIAAEIRQGRRNAVSFVTAAIAAIEVRDTRVNSVTRIF
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHEEEECCHHHHHHHHH
GPRAVAEAEKIDRRIAAGEDPGPLAGVPFGVKDLFDVEGEVTTAGSVVLREARPAVQDAT
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCCEECCCHHHHHHCCCCHHHHH
VIARLRAAGAIPLASLNMDEFAYGFATDNAHYGITRNPHDPARLAGGSSGGSAAAVAAGF
HHHHHHHCCCCEEEECCHHHHEEEEECCCCCCCCCCCCCCHHHHCCCCCCCCHHHHHHCC
LPFTLGSDTNGSIRVPASLCGVWGLKPTFGTVPREGAYPFAASLDVVGPFAGTLADLRVV
EEEEECCCCCCCEECCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
HEAMNGAPMPALPVLSDLRVARLGGWFAENVSAPLAAAIDAVAGHLGHLPVVELPEVARA
HHHHCCCCCCHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHH
RAASFLITAAEGGMLHLPRLRRRALQYDPATRDRLIAGALLPAATVLQAHRFRAWFREQI
HHHEEEEEECCCCEEECHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HAAFRTADVLIAPATVGEAPLIDQPTIVVDGQAVSARANLGLYTQPLSLAGVPILSVPLL
HHHHHHHCEEEECCCCCCCCCCCCCEEEECCCEEEEECCCCEEECCHHHCCCCEEEECEE
GTGGLPLGLQLIAAPGGEPALFAVAAALEAAGVAGKVPLPKGR
CCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA