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The map label for this gene is pucL [H]

Identifier: 162147776

GI number: 162147776

Start: 2026399

End: 2027277

Strand: Reverse

Name: pucL [H]

Synonym: GDI_1992

Alternate gene names: 162147776

Gene position: 2027277-2026399 (Counterclockwise)

Preceding gene: 162147777

Following gene: 162147771

Centisome position: 51.4

GC content: 68.94

Gene sequence:

>879_bases
ATGACCATCGGCATCATGGACCGGGTCAACCGGCTGGACCCGGCGGATTTCGTCGCCCTGTTCGGCGCGCTGTACGAACA
TTCGCCCTGGGTCGCGGCACGGGCGGCGTCCCTGCGGCCTTTTCCGGACCCGGACGCGATGCTGGCGGCCATGAACCAGG
TGCTGAATCGGGCCACCGACGCCGAGAAGCTGGCGCTGGTGCGCGCGCATCCCGAACTGGCGCGGCGGGCCGGGGTGGAC
CCGACCCTGACACAGGCCTCGGCCGCCGAGCAGGCATCCGCCGGGCTGGACCGTCTGTCGCCCGAGGAATACGCCCGGTT
CAACCGCCTGAACGACGCCTACGCCGCGCGCTTCGCCATGCCGTTCGTGATCTGCGTGCGGTTGTCGGACAAGGATTTCA
TCCTGTCCGAGATGGAACGCCGCGTCGTCCACACGCCGGAGCAGGAGGTGCGGACCGCGATCGTGGAAATCGGCAAGATC
GCCAGGCTGCGCCTGGCCGACGCCCTGGCGCGGCTGGAAAAGGAAGCCGTGATCACCCTGTCCAGCCATGTGCTGGACCT
GGTCTCCGGCCGGCCGGCCGCCGGGATGGACATCGCGTTGTGGTCGGGCGCCACACGCCTGTTCAGCGGGCGAACCAACG
GGGATGGGCGTTGTCCGGACCTGGCCGGGATCGGTGCCCTGGCACCGGGCGCCTATCGTCTGGAATTCGGGGTTGCGGCG
TATTTCCGGGGGCAGGGCGTGGCGCTGAGCGACCCGCCGTTCCTGGATATCGTGCCGATCGCCTTCGGCATCGCCCCGCC
TGTGGACGGGAAGGGCGGGCACTATCATGTGCCGCTGCTGGTCTCCCCGTACGGGTTTTCGACCTATCGGGGAAGCTGA

Upstream 100 bases:

>100_bases
GGGCGGTGGCGCGCTTCCTGGACCATGTCATGGCGCATGATCGCGTCTGGGTCGCCACCCGGCTGGATATAGCCCGCCAT
TGGCTGGAGGTTCACCCCGC

Downstream 100 bases:

>100_bases
GAGAGGTTGCCGGCCCGGGAAAACCGGGCCGGCATTCCGGGTCAGACCAGCTTCTGCTTCAGCGCCGCGGTCAGTTCGCG
CGTGCCGGCGGTGCCGCCCA

Product: hypothetical protein

Products: NA

Alternate protein names: 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase; OHCU decarboxylase; Uricase; Urate oxidase [H]

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MTIGIMDRVNRLDPADFVALFGALYEHSPWVAARAASLRPFPDPDAMLAAMNQVLNRATDAEKLALVRAHPELARRAGVD
PTLTQASAAEQASAGLDRLSPEEYARFNRLNDAYAARFAMPFVICVRLSDKDFILSEMERRVVHTPEQEVRTAIVEIGKI
ARLRLADALARLEKEAVITLSSHVLDLVSGRPAAGMDIALWSGATRLFSGRTNGDGRCPDLAGIGALAPGAYRLEFGVAA
YFRGQGVALSDPPFLDIVPIAFGIAPPVDGKGGHYHVPLLVSPYGFSTYRGS

Sequences:

>Translated_292_residues
MTIGIMDRVNRLDPADFVALFGALYEHSPWVAARAASLRPFPDPDAMLAAMNQVLNRATDAEKLALVRAHPELARRAGVD
PTLTQASAAEQASAGLDRLSPEEYARFNRLNDAYAARFAMPFVICVRLSDKDFILSEMERRVVHTPEQEVRTAIVEIGKI
ARLRLADALARLEKEAVITLSSHVLDLVSGRPAAGMDIALWSGATRLFSGRTNGDGRCPDLAGIGALAPGAYRLEFGVAA
YFRGQGVALSDPPFLDIVPIAFGIAPPVDGKGGHYHVPLLVSPYGFSTYRGS
>Mature_291_residues
TIGIMDRVNRLDPADFVALFGALYEHSPWVAARAASLRPFPDPDAMLAAMNQVLNRATDAEKLALVRAHPELARRAGVDP
TLTQASAAEQASAGLDRLSPEEYARFNRLNDAYAARFAMPFVICVRLSDKDFILSEMERRVVHTPEQEVRTAIVEIGKIA
RLRLADALARLEKEAVITLSSHVLDLVSGRPAAGMDIALWSGATRLFSGRTNGDGRCPDLAGIGALAPGAYRLEFGVAAY
FRGQGVALSDPPFLDIVPIAFGIAPPVDGKGGHYHVPLLVSPYGFSTYRGS

Specific function: Catalyzes two steps in the degradation of uric acid, i.e. the oxidation of uric acid to 5-hydroxyisourate (HIU) and the stereoselective decarboxylation of 2-oxo-4-hydroxy-4-carboxy-5- ureidoimidazoline (OHCU) to (S)-allantoin [H]

COG id: COG3195

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the uricase family [H]

Homologues:

Organism=Homo sapiens, GI157743280, Length=164, Percent_Identity=37.8048780487805, Blast_Score=106, Evalue=3e-23,
Organism=Escherichia coli, GI1788281, Length=117, Percent_Identity=36.7521367521368, Blast_Score=76, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI115532920, Length=134, Percent_Identity=35.0746268656716, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI115534555, Length=118, Percent_Identity=38.135593220339, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI115532922, Length=118, Percent_Identity=35.5932203389831, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018020
- InterPro:   IPR017580
- InterPro:   IPR002042
- InterPro:   IPR019842 [H]

Pfam domain/function: PF09349 OHCU_decarbox; PF01014 Uricase [H]

EC number: =1.7.3.3 [H]

Molecular weight: Translated: 31496; Mature: 31365

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00092 N6_MTASE ; PS00768 TRANSTHYRETIN_1 ; PS00769 TRANSTHYRETIN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIGIMDRVNRLDPADFVALFGALYEHSPWVAARAASLRPFPDPDAMLAAMNQVLNRATD
CEECHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHCCC
AEKLALVRAHPELARRAGVDPTLTQASAAEQASAGLDRLSPEEYARFNRLNDAYAARFAM
HHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHCCHHCCCHHHHHHHHHCCHHHHHHHHC
PFVICVRLSDKDFILSEMERRVVHTPEQEVRTAIVEIGKIARLRLADALARLEKEAVITL
CEEEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSHVLDLVSGRPAAGMDIALWSGATRLFSGRTNGDGRCPDLAGIGALAPGAYRLEFGVAA
HHHHHHHHCCCCCCCCEEEEECCCHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEECEEE
YFRGQGVALSDPPFLDIVPIAFGIAPPVDGKGGHYHVPLLVSPYGFSTYRGS
EECCCCCEECCCCCEEHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
TIGIMDRVNRLDPADFVALFGALYEHSPWVAARAASLRPFPDPDAMLAAMNQVLNRATD
EECHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHCCC
AEKLALVRAHPELARRAGVDPTLTQASAAEQASAGLDRLSPEEYARFNRLNDAYAARFAM
HHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHCCHHCCCHHHHHHHHHCCHHHHHHHHC
PFVICVRLSDKDFILSEMERRVVHTPEQEVRTAIVEIGKIARLRLADALARLEKEAVITL
CEEEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSHVLDLVSGRPAAGMDIALWSGATRLFSGRTNGDGRCPDLAGIGALAPGAYRLEFGVAA
HHHHHHHHCCCCCCCCEEEEECCCHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEECEEE
YFRGQGVALSDPPFLDIVPIAFGIAPPVDGKGGHYHVPLLVSPYGFSTYRGS
EECCCCCEECCCCCEEHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377; 11344136 [H]