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The map label for this gene is tpiA

Identifier: 162147716

GI number: 162147716

Start: 1965696

End: 1966439

Strand: Reverse

Name: tpiA

Synonym: GDI_1932

Alternate gene names: 162147716

Gene position: 1966439-1965696 (Counterclockwise)

Preceding gene: 162147730

Following gene: 162147715

Centisome position: 49.86

GC content: 68.28

Gene sequence:

>744_bases
ATGAGGCAGATGATCGTCGGCAACTGGAAGATGAACGGACTGGGCGCCCCTTCGCGCGACCTGGTCGGGGAGATCGCGGA
GGGCCTGGCCACCATCCCCTCGCCTCCGCAGGTCGTGGTCTGTCCCCCGTTCACCCAACTGGCCGGGATCGGCCCCCTCC
TGAAGGGCAGCGGCATCGCACTGGGGGCGCAGGACTGCCATCAGGCGGCGTCGGGCGCGCATACCGGCGACATATCCGCC
GCCATGCTTGCCGACCTTGGCGTGGAATATGTCGTGCTGGGTCATTCCGAACGGCGCCGCGACCACGGCGAGCTGGACGA
GACGGTGCGCGAGAAGACGCAGACCGCCCTGGCCGCCGGACTGACCCCGATCGTCTGCATCGGAGAGACCGGCGACCAGA
AGGCGTCCGGCGAAAGCCGCGACGCGATCGGCTGGCAGATCCAGGGATCGCTGCCCGACGGTTTCAGCGGCGTCGTGGCC
TACGAGCCCGTATGGGCGATCGGATCGGGCAACCCGGCCGCCAGCCAGGACATCGCGGACATGATGGGCTTCATCCGCGC
CGAGCTGGTGCGTCAGTTCGGTGCGGCTGGAAAAACGATTCGGATCCTTTATGGTGGATCGGTCAACGGGCGCGACGCGG
CGTCCATCCTGCCGATCGCCGAGGTCGGCGGCGCGCTGGTCGGCAGCGCAAGCCTGCAGGCCGACACGTTTCTGCCGATT
GTGCGGGCCGCCGTGGACCTGTAA

Upstream 100 bases:

>100_bases
TGTAGGATGTTATAACCCGGTCGGCAGTACCCCTGAAAAAAACACAAGATCGCCCGAGACCCGACCGAAGGGCGGCGCGG
AAACACAGGGATCGAGACAC

Downstream 100 bases:

>100_bases
ACGGTCCTTTTGCTGTGCCTTGCTTGGGCGGCGCCTTACCTGGAAGCGAGTATGATTACCGTCCTTCTTTTTCTGCATCT
GTTCGTAACCCTGGCCCTGA

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MRQMIVGNWKMNGLGAPSRDLVGEIAEGLATIPSPPQVVVCPPFTQLAGIGPLLKGSGIALGAQDCHQAASGAHTGDISA
AMLADLGVEYVVLGHSERRRDHGELDETVREKTQTALAAGLTPIVCIGETGDQKASGESRDAIGWQIQGSLPDGFSGVVA
YEPVWAIGSGNPAASQDIADMMGFIRAELVRQFGAAGKTIRILYGGSVNGRDAASILPIAEVGGALVGSASLQADTFLPI
VRAAVDL

Sequences:

>Translated_247_residues
MRQMIVGNWKMNGLGAPSRDLVGEIAEGLATIPSPPQVVVCPPFTQLAGIGPLLKGSGIALGAQDCHQAASGAHTGDISA
AMLADLGVEYVVLGHSERRRDHGELDETVREKTQTALAAGLTPIVCIGETGDQKASGESRDAIGWQIQGSLPDGFSGVVA
YEPVWAIGSGNPAASQDIADMMGFIRAELVRQFGAAGKTIRILYGGSVNGRDAASILPIAEVGGALVGSASLQADTFLPI
VRAAVDL
>Mature_247_residues
MRQMIVGNWKMNGLGAPSRDLVGEIAEGLATIPSPPQVVVCPPFTQLAGIGPLLKGSGIALGAQDCHQAASGAHTGDISA
AMLADLGVEYVVLGHSERRRDHGELDETVREKTQTALAAGLTPIVCIGETGDQKASGESRDAIGWQIQGSLPDGFSGVVA
YEPVWAIGSGNPAASQDIADMMGFIRAELVRQFGAAGKTIRILYGGSVNGRDAASILPIAEVGGALVGSASLQADTFLPI
VRAAVDL

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI4507645, Length=247, Percent_Identity=38.8663967611336, Blast_Score=148, Evalue=4e-36,
Organism=Homo sapiens, GI226529917, Length=247, Percent_Identity=38.8663967611336, Blast_Score=148, Evalue=5e-36,
Organism=Escherichia coli, GI1790353, Length=250, Percent_Identity=40, Blast_Score=169, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17536593, Length=247, Percent_Identity=41.2955465587045, Blast_Score=162, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6320255, Length=243, Percent_Identity=41.9753086419753, Blast_Score=170, Evalue=2e-43,
Organism=Drosophila melanogaster, GI28572008, Length=249, Percent_Identity=38.1526104417671, Blast_Score=146, Evalue=1e-35,
Organism=Drosophila melanogaster, GI28572006, Length=249, Percent_Identity=38.1526104417671, Blast_Score=146, Evalue=1e-35,
Organism=Drosophila melanogaster, GI28572004, Length=249, Percent_Identity=38.1526104417671, Blast_Score=146, Evalue=1e-35,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_GLUDA (A9HJ86)

Other databases:

- EMBL:   AM889285
- EMBL:   CP001189
- RefSeq:   YP_001602177.1
- RefSeq:   YP_002274569.1
- ProteinModelPortal:   A9HJ86
- SMR:   A9HJ86
- GeneID:   5790186
- GeneID:   6973546
- GenomeReviews:   AM889285_GR
- GenomeReviews:   CP001189_GR
- KEGG:   gdj:Gdia_0154
- HOGENOM:   HBG708281
- OMA:   HANTSPE
- ProtClustDB:   PRK00042
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 25366; Mature: 25366

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 95-95 ACT_SITE 162-162 BINDING 8-8 BINDING 10-10

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQMIVGNWKMNGLGAPSRDLVGEIAEGLATIPSPPQVVVCPPFTQLAGIGPLLKGSGIA
CCCEEEECEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHCCCCCCCCCCCE
LGAQDCHQAASGAHTGDISAAMLADLGVEYVVLGHSERRRDHGELDETVREKTQTALAAG
ECHHHHHHHHCCCCCCCHHHHHHHHCCCEEEEECCCHHCCCCCHHHHHHHHHHHHHHHHC
LTPIVCIGETGDQKASGESRDAIGWQIQGSLPDGFSGVVAYEPVWAIGSGNPAASQDIAD
CCEEEEECCCCCCCCCCCCCCCCCEEEECCCCCCCCCEEEECCEEEECCCCCCCHHHHHH
MMGFIRAELVRQFGAAGKTIRILYGGSVNGRDAASILPIAEVGGALVGSASLQADTFLPI
HHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHCCCHHHHCCHHCCCCCCCCHHHHHH
VRAAVDL
HHHHHCC
>Mature Secondary Structure
MRQMIVGNWKMNGLGAPSRDLVGEIAEGLATIPSPPQVVVCPPFTQLAGIGPLLKGSGIA
CCCEEEECEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHCCCCCCCCCCCE
LGAQDCHQAASGAHTGDISAAMLADLGVEYVVLGHSERRRDHGELDETVREKTQTALAAG
ECHHHHHHHHCCCCCCCHHHHHHHHCCCEEEEECCCHHCCCCCHHHHHHHHHHHHHHHHC
LTPIVCIGETGDQKASGESRDAIGWQIQGSLPDGFSGVVAYEPVWAIGSGNPAASQDIAD
CCEEEEECCCCCCCCCCCCCCCCCEEEECCCCCCCCCEEEECCEEEECCCCCCCHHHHHH
MMGFIRAELVRQFGAAGKTIRILYGGSVNGRDAASILPIAEVGGALVGSASLQADTFLPI
HHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHCCCHHHHCCHHCCCCCCCCHHHHHH
VRAAVDL
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA